Gene expression at early stages of Brassica napus seed development as revealed by transcript profiling of seed-abundant cDNAs
Approximately 5,000 plaques derived from a Brassica napus L. (canola) seed-cDNA library representing 15 days after pollination (DAP) were differentially screened for highly expressed genes at the early stages of seed development. Analysis of 104 differentially expressed sequence tags revealed 54 uni...
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| Veröffentlicht in: | Planta 2004-01, Vol.218 (3), p.483-491 |
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| creator | Dong, Jinzhuo Keller, Wilf A. Yan, Wei Georges, Fawzy |
| description | Approximately 5,000 plaques derived from a Brassica napus L. (canola) seed-cDNA library representing 15 days after pollination (DAP) were differentially screened for highly expressed genes at the early stages of seed development. Analysis of 104 differentially expressed sequence tags revealed 54 unique genes, of which 33 had putative homologues described in Arabidopsis thaliana (L.) Heynh. or B. napus. These encoded diverse proteins, ranging from proteins of unknown function to metabolic enzymes and proteins associated with cell structure and development. Twenty-five genes were only expressed in seeds, and 11 of these started to express as early as 5 or 10 DAP. The majority of the seed-specific genes that are expressed at early stages of seed development encoded proteins with high similarity to hypothetical Arabidopsis proteins. Tissue-specificity determined by Northern analysis revealed that four seed-specific genes were expressed only in seed coats and another five in both embryos and seed coats. Analysis of transcript profiles of seed-abundant as well as seed-specific genes, and their expression patterns, implies that the B. napus seed is undergoing an active cell proliferation during 10—20 DAP, while establishing metabolic networks for subsequent seed maturation. |
| doi_str_mv | 10.1007/s00425-003-1124-2 |
| format | Article |
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(canola) seed-cDNA library representing 15 days after pollination (DAP) were differentially screened for highly expressed genes at the early stages of seed development. Analysis of 104 differentially expressed sequence tags revealed 54 unique genes, of which 33 had putative homologues described in Arabidopsis thaliana (L.) Heynh. or B. napus. These encoded diverse proteins, ranging from proteins of unknown function to metabolic enzymes and proteins associated with cell structure and development. Twenty-five genes were only expressed in seeds, and 11 of these started to express as early as 5 or 10 DAP. The majority of the seed-specific genes that are expressed at early stages of seed development encoded proteins with high similarity to hypothetical Arabidopsis proteins. Tissue-specificity determined by Northern analysis revealed that four seed-specific genes were expressed only in seed coats and another five in both embryos and seed coats. Analysis of transcript profiles of seed-abundant as well as seed-specific genes, and their expression patterns, implies that the B. napus seed is undergoing an active cell proliferation during 10—20 DAP, while establishing metabolic networks for subsequent seed maturation.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s00425-003-1124-2</identifier><identifier>PMID: 14574574</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>Agronomy. Soil science and plant productions ; Arabidopsis - genetics ; Arabidopsis Proteins - genetics ; Biological and medical sciences ; Blotting, Northern ; Brassica napus - genetics ; Brassica napus - growth & development ; Complementary DNA ; DNA, Complementary - genetics ; DNA, Plant - genetics ; Economic plant physiology ; Embryos ; Enzymes ; Enzymes - genetics ; Expressed Sequence Tags ; Fructification and ripening ; Fructification, ripening. Postharvest physiology ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Plant - genetics ; Genes ; Genetics ; Growth and development ; Messenger RNA ; Models, Genetic ; Plant cells ; Plant physiology and development ; Plant Proteins - genetics ; Proteins ; Seed development ; Seeds ; Seeds - genetics ; Testa ; Transcription, Genetic - genetics ; Vegetative and sexual reproduction, floral biology, fructification</subject><ispartof>Planta, 2004-01, Vol.218 (3), p.483-491</ispartof><rights>Springer-Verlag Berlin Heidelberg 2004</rights><rights>2004 INIST-CNRS</rights><rights>Springer-Verlag 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-93c5e0a6adf309e1786c66fc672a9795e30882443782e3099dfd425301688d323</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23388277$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23388277$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,777,781,800,4010,27904,27905,27906,57998,58231</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15433468$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14574574$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dong, Jinzhuo</creatorcontrib><creatorcontrib>Keller, Wilf A.</creatorcontrib><creatorcontrib>Yan, Wei</creatorcontrib><creatorcontrib>Georges, Fawzy</creatorcontrib><title>Gene expression at early stages of Brassica napus seed development as revealed by transcript profiling of seed-abundant cDNAs</title><title>Planta</title><addtitle>Planta</addtitle><description>Approximately 5,000 plaques derived from a Brassica napus L. (canola) seed-cDNA library representing 15 days after pollination (DAP) were differentially screened for highly expressed genes at the early stages of seed development. Analysis of 104 differentially expressed sequence tags revealed 54 unique genes, of which 33 had putative homologues described in Arabidopsis thaliana (L.) Heynh. or B. napus. These encoded diverse proteins, ranging from proteins of unknown function to metabolic enzymes and proteins associated with cell structure and development. Twenty-five genes were only expressed in seeds, and 11 of these started to express as early as 5 or 10 DAP. The majority of the seed-specific genes that are expressed at early stages of seed development encoded proteins with high similarity to hypothetical Arabidopsis proteins. Tissue-specificity determined by Northern analysis revealed that four seed-specific genes were expressed only in seed coats and another five in both embryos and seed coats. Analysis of transcript profiles of seed-abundant as well as seed-specific genes, and their expression patterns, implies that the B. napus seed is undergoing an active cell proliferation during 10—20 DAP, while establishing metabolic networks for subsequent seed maturation.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Brassica napus - genetics</subject><subject>Brassica napus - growth & development</subject><subject>Complementary DNA</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Plant - genetics</subject><subject>Economic plant physiology</subject><subject>Embryos</subject><subject>Enzymes</subject><subject>Enzymes - genetics</subject><subject>Expressed Sequence Tags</subject><subject>Fructification and ripening</subject><subject>Fructification, ripening. Postharvest physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>Genes</subject><subject>Genetics</subject><subject>Growth and development</subject><subject>Messenger RNA</subject><subject>Models, Genetic</subject><subject>Plant cells</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - genetics</subject><subject>Proteins</subject><subject>Seed development</subject><subject>Seeds</subject><subject>Seeds - genetics</subject><subject>Testa</subject><subject>Transcription, Genetic - genetics</subject><subject>Vegetative and sexual reproduction, floral biology, fructification</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkU1v1DAQhi0EotvCD-AAspDgFhh_JHaObSkFqYILnK1ZZ1JllU2CJ0Hsgf-Ow66ohGTJH-8zr-13hHih4J0CcO8ZwOqyADCFUtoW-pHYKGt0ocH6x2KTBV1Abcozcc68A8iic0_FmbKlW8dG_L6lgST9mhIxd-MgcZaEqT9InvGeWI6tvEqYtYhywGlhyUSNbOgn9eO0p2GWyDLlLfb5fHuQc8KBY-qmWU5pbLu-G-5Xm7WuwO0yNJiL4ocvl_xMPGmxZ3p-mi_E9483364_FXdfbz9fX94V0Vo1F7WJJQFW2LQGalLOV7Gq2lg5jbWrSzLgvbb5c17ndV03bZODMaAq7xujzYV4e_TND_qxEM9h33GkvseBxoWDB_DW_QVf_wfuxiUN-W3Ba_CVctZnSB2hmEbmRG2YUrfHdAgKwtqYcGxMyPmHtTFhNX51Ml62e2oeKk6dyMCbE4AcsW9ziLHjB660xthqvfzlkdvxPKZ_ujYmR-Cc-QN1YZ7R</recordid><startdate>20040101</startdate><enddate>20040101</enddate><creator>Dong, Jinzhuo</creator><creator>Keller, Wilf A.</creator><creator>Yan, Wei</creator><creator>Georges, Fawzy</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040101</creationdate><title>Gene expression at early stages of Brassica napus seed development as revealed by transcript profiling of seed-abundant cDNAs</title><author>Dong, Jinzhuo ; Keller, Wilf A. ; Yan, Wei ; Georges, Fawzy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-93c5e0a6adf309e1786c66fc672a9795e30882443782e3099dfd425301688d323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Brassica napus - genetics</topic><topic>Brassica napus - growth & development</topic><topic>Complementary DNA</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Plant - genetics</topic><topic>Economic plant physiology</topic><topic>Embryos</topic><topic>Enzymes</topic><topic>Enzymes - genetics</topic><topic>Expressed Sequence Tags</topic><topic>Fructification and ripening</topic><topic>Fructification, ripening. Postharvest physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant - genetics</topic><topic>Genes</topic><topic>Genetics</topic><topic>Growth and development</topic><topic>Messenger RNA</topic><topic>Models, Genetic</topic><topic>Plant cells</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - genetics</topic><topic>Proteins</topic><topic>Seed development</topic><topic>Seeds</topic><topic>Seeds - genetics</topic><topic>Testa</topic><topic>Transcription, Genetic - genetics</topic><topic>Vegetative and sexual reproduction, floral biology, fructification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dong, Jinzhuo</creatorcontrib><creatorcontrib>Keller, Wilf A.</creatorcontrib><creatorcontrib>Yan, Wei</creatorcontrib><creatorcontrib>Georges, Fawzy</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dong, Jinzhuo</au><au>Keller, Wilf A.</au><au>Yan, Wei</au><au>Georges, Fawzy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene expression at early stages of Brassica napus seed development as revealed by transcript profiling of seed-abundant cDNAs</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>2004-01-01</date><risdate>2004</risdate><volume>218</volume><issue>3</issue><spage>483</spage><epage>491</epage><pages>483-491</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>Approximately 5,000 plaques derived from a Brassica napus L. (canola) seed-cDNA library representing 15 days after pollination (DAP) were differentially screened for highly expressed genes at the early stages of seed development. Analysis of 104 differentially expressed sequence tags revealed 54 unique genes, of which 33 had putative homologues described in Arabidopsis thaliana (L.) Heynh. or B. napus. These encoded diverse proteins, ranging from proteins of unknown function to metabolic enzymes and proteins associated with cell structure and development. Twenty-five genes were only expressed in seeds, and 11 of these started to express as early as 5 or 10 DAP. The majority of the seed-specific genes that are expressed at early stages of seed development encoded proteins with high similarity to hypothetical Arabidopsis proteins. Tissue-specificity determined by Northern analysis revealed that four seed-specific genes were expressed only in seed coats and another five in both embryos and seed coats. Analysis of transcript profiles of seed-abundant as well as seed-specific genes, and their expression patterns, implies that the B. napus seed is undergoing an active cell proliferation during 10—20 DAP, while establishing metabolic networks for subsequent seed maturation.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><pmid>14574574</pmid><doi>10.1007/s00425-003-1124-2</doi><tpages>9</tpages></addata></record> |
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| subjects | Agronomy. Soil science and plant productions Arabidopsis - genetics Arabidopsis Proteins - genetics Biological and medical sciences Blotting, Northern Brassica napus - genetics Brassica napus - growth & development Complementary DNA DNA, Complementary - genetics DNA, Plant - genetics Economic plant physiology Embryos Enzymes Enzymes - genetics Expressed Sequence Tags Fructification and ripening Fructification, ripening. Postharvest physiology Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Profiling Gene Expression Regulation, Plant - genetics Genes Genetics Growth and development Messenger RNA Models, Genetic Plant cells Plant physiology and development Plant Proteins - genetics Proteins Seed development Seeds Seeds - genetics Testa Transcription, Genetic - genetics Vegetative and sexual reproduction, floral biology, fructification |
| title | Gene expression at early stages of Brassica napus seed development as revealed by transcript profiling of seed-abundant cDNAs |
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