Synthesis of the measles virus nucleoprotein in yeast Pichia pastoris and Saccharomyces cerevisiae

The development of a simple, efficient and cost-effective system for generation of measles virus nucleoprotein might help to upgrade reagents for measles serology. The gene encoding measles nucleoprotein was successfully expressed in two different yeast genera, Pichia pastoris and Saccharomyces cere...

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Veröffentlicht in:	Journal of biotechnology 2004-01, Vol.107 (2), p.115-124
Hauptverfasser:	Slibinskas, Rimantas, Samuel, Dhanraj, Gedvilaite, Alma, Staniulis, Juozas, Sasnauskas, Kestutis
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Blotting, Western Cell Compartmentation Electrophoresis, Polyacrylamide Gel Escherichia coli Expression Fundamental and applied biological sciences. Psychology Gene Dosage Green Fluorescent Proteins Humans Immunoglobulin M - blood Kinetics Luminescent Proteins Measles Measles - virology Measles virus Measles virus - chemistry Measles virus - immunology Measles virus - metabolism Nucleocapsid-like particles Nucleoproteins - biosynthesis Nucleoproteins - genetics Nucleoproteins - immunology Nucleoproteins - isolation & purification Nucleoproteins - metabolism Nucleoproteins - ultrastructure Pichia - metabolism Pichia pastoris Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - metabolism Spectrometry, Fluorescence
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