Direct evidence for cyanide-insensitive quinol oxidase (alternative oxidase) in apicomplexan parasite Cryptosporidium parvum: phylogenetic and therapeutic implications
Cryptosporidium parvum is a parasitic protozoan that causes the diarrheal disease cryptosporidiosis, for which no satisfactory chemotherapy is currently available. Although the presence of mitochondria in this parasite has been suggested, its respiratory system is poorly understood due to difficulti...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 2004-01, Vol.313 (4), p.1044-1052 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1052 |
|---|---|
| container_issue | 4 |
| container_start_page | 1044 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 313 |
| creator | Suzuki, Takashi Hashimoto, Tetsuo Yabu, Yoshisada Kido, Yasutoshi Sakamoto, Kimitoshi Nihei, Coh-ichi Hato, Mariko Suzuki, Shu-ichi Amano, Yuko Nagai, Kazuo Hosokawa, Tomoyoshi Minagawa, Nobuko Ohta, Nobuo Kita, Kiyoshi |
| description | Cryptosporidium parvum is a parasitic protozoan that causes the diarrheal disease cryptosporidiosis, for which no satisfactory chemotherapy is currently available. Although the presence of mitochondria in this parasite has been suggested, its respiratory system is poorly understood due to difficulties in performing biochemical analyses. In order to better understand the respiratory chain of
C. parvum, we surveyed its genomic DNA database in GenBank and identified a partial sequence encoding cyanide-insensitive alternative oxidase (AOX). Based on this sequence, we cloned
C. parvum AOX (
CpAOX) cDNA from the phylum apicomplexa for the first time. The deduced amino acid sequence (335 a.a.) of CpAOX contains diiron coordination motifs (–E–, –EXXH–) that are conserved among AOXs. Phylogenetic analysis suggested that CpAOX is a mitochondrial-type AOX, possibly derived from mitochondrial endosymbiont gene transfer. The recombinant enzyme expressed in
Escherichia coli showed quinol oxidase activity. This activity was insensitive to cyanide and highly sensitive to ascofuranone, a specific inhibitor of trypanosome AOX. |
| doi_str_mv | 10.1016/j.bbrc.2003.12.038 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80083178</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X03026214</els_id><sourcerecordid>80083178</sourcerecordid><originalsourceid>FETCH-LOGICAL-c383t-3b740e97477c175c17d7f49b5ef673c8e43a6d1b6d25bc658a7049296f854baa3</originalsourceid><addsrcrecordid>eNqFkcFu1DAQhiMEokvhBTggnxAcsoxjx04QF7S0gFSJC0jcLMeeUK8SO7WTVfeJeE2c7krc4DAajef__5H8FcVLClsKVLzbb7summ0FwLa02gJrHhUbCi2UFQX-uNgAgCirlv68KJ6ltAeglIv2aXFBuQQheLMpfn9yEc1M8OAseoOkD5GYo_Z5LJ1P6JOb3QHJ3eJ8GEi4d1YnJG_0MGP0-mF3fnxLnCd6ciaM04D32pNJR539SHbxOM0hTSE665ZxXRyW8T2Zbo9D-IUeZ2eI9pbMtxj1hMs6uxzjTD4RfHpePOn1kPDFuV8WP66vvu--lDffPn_dfbwpDWvYXLJOcsBWcikNlXUuK3vedjX2QjLTIGdaWNoJW9WdEXWjJfC2akXf1LzTml0Wr0-5Uwx3C6ZZjS4ZHAbtMSxJNQANo7L5r5DKlnNgMgurk9DEkFLEXk3RjToeFQW1clR7tXJUK0dFK5U5ZtOrc_rSjWj_Ws7gsuDDSYD5Mw4Oo0rGrQDtA09lg_tX_h-TirPd</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17944037</pqid></control><display><type>article</type><title>Direct evidence for cyanide-insensitive quinol oxidase (alternative oxidase) in apicomplexan parasite Cryptosporidium parvum: phylogenetic and therapeutic implications</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Suzuki, Takashi ; Hashimoto, Tetsuo ; Yabu, Yoshisada ; Kido, Yasutoshi ; Sakamoto, Kimitoshi ; Nihei, Coh-ichi ; Hato, Mariko ; Suzuki, Shu-ichi ; Amano, Yuko ; Nagai, Kazuo ; Hosokawa, Tomoyoshi ; Minagawa, Nobuko ; Ohta, Nobuo ; Kita, Kiyoshi</creator><creatorcontrib>Suzuki, Takashi ; Hashimoto, Tetsuo ; Yabu, Yoshisada ; Kido, Yasutoshi ; Sakamoto, Kimitoshi ; Nihei, Coh-ichi ; Hato, Mariko ; Suzuki, Shu-ichi ; Amano, Yuko ; Nagai, Kazuo ; Hosokawa, Tomoyoshi ; Minagawa, Nobuko ; Ohta, Nobuo ; Kita, Kiyoshi</creatorcontrib><description>Cryptosporidium parvum is a parasitic protozoan that causes the diarrheal disease cryptosporidiosis, for which no satisfactory chemotherapy is currently available. Although the presence of mitochondria in this parasite has been suggested, its respiratory system is poorly understood due to difficulties in performing biochemical analyses. In order to better understand the respiratory chain of
C. parvum, we surveyed its genomic DNA database in GenBank and identified a partial sequence encoding cyanide-insensitive alternative oxidase (AOX). Based on this sequence, we cloned
C. parvum AOX (
CpAOX) cDNA from the phylum apicomplexa for the first time. The deduced amino acid sequence (335 a.a.) of CpAOX contains diiron coordination motifs (–E–, –EXXH–) that are conserved among AOXs. Phylogenetic analysis suggested that CpAOX is a mitochondrial-type AOX, possibly derived from mitochondrial endosymbiont gene transfer. The recombinant enzyme expressed in
Escherichia coli showed quinol oxidase activity. This activity was insensitive to cyanide and highly sensitive to ascofuranone, a specific inhibitor of trypanosome AOX.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2003.12.038</identifier><identifier>PMID: 14706648</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Alternative oxidase ; Amino Acid Sequence ; Animals ; Ascofuranone ; Base Sequence ; Cloning, Molecular ; Cryptosporidiosis - drug therapy ; Cryptosporidium parvum ; Cryptosporidium parvum - drug effects ; Cryptosporidium parvum - enzymology ; Cryptosporidium parvum - genetics ; DNA, Protozoan - genetics ; Enzyme Inhibitors - pharmacology ; Humans ; Mitochondria - enzymology ; Molecular Sequence Data ; Oxidoreductases - antagonists & inhibitors ; Oxidoreductases - chemistry ; Oxidoreductases - genetics ; Oxidoreductases - metabolism ; Phylogenetic analysis ; Phylogeny ; Recombinant Proteins - antagonists & inhibitors ; Recombinant Proteins - chemistry ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Sequence Homology, Amino Acid ; Sesquiterpenes - pharmacology</subject><ispartof>Biochemical and biophysical research communications, 2004-01, Vol.313 (4), p.1044-1052</ispartof><rights>2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-3b740e97477c175c17d7f49b5ef673c8e43a6d1b6d25bc658a7049296f854baa3</citedby><cites>FETCH-LOGICAL-c383t-3b740e97477c175c17d7f49b5ef673c8e43a6d1b6d25bc658a7049296f854baa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2003.12.038$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14706648$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suzuki, Takashi</creatorcontrib><creatorcontrib>Hashimoto, Tetsuo</creatorcontrib><creatorcontrib>Yabu, Yoshisada</creatorcontrib><creatorcontrib>Kido, Yasutoshi</creatorcontrib><creatorcontrib>Sakamoto, Kimitoshi</creatorcontrib><creatorcontrib>Nihei, Coh-ichi</creatorcontrib><creatorcontrib>Hato, Mariko</creatorcontrib><creatorcontrib>Suzuki, Shu-ichi</creatorcontrib><creatorcontrib>Amano, Yuko</creatorcontrib><creatorcontrib>Nagai, Kazuo</creatorcontrib><creatorcontrib>Hosokawa, Tomoyoshi</creatorcontrib><creatorcontrib>Minagawa, Nobuko</creatorcontrib><creatorcontrib>Ohta, Nobuo</creatorcontrib><creatorcontrib>Kita, Kiyoshi</creatorcontrib><title>Direct evidence for cyanide-insensitive quinol oxidase (alternative oxidase) in apicomplexan parasite Cryptosporidium parvum: phylogenetic and therapeutic implications</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Cryptosporidium parvum is a parasitic protozoan that causes the diarrheal disease cryptosporidiosis, for which no satisfactory chemotherapy is currently available. Although the presence of mitochondria in this parasite has been suggested, its respiratory system is poorly understood due to difficulties in performing biochemical analyses. In order to better understand the respiratory chain of
C. parvum, we surveyed its genomic DNA database in GenBank and identified a partial sequence encoding cyanide-insensitive alternative oxidase (AOX). Based on this sequence, we cloned
C. parvum AOX (
CpAOX) cDNA from the phylum apicomplexa for the first time. The deduced amino acid sequence (335 a.a.) of CpAOX contains diiron coordination motifs (–E–, –EXXH–) that are conserved among AOXs. Phylogenetic analysis suggested that CpAOX is a mitochondrial-type AOX, possibly derived from mitochondrial endosymbiont gene transfer. The recombinant enzyme expressed in
Escherichia coli showed quinol oxidase activity. This activity was insensitive to cyanide and highly sensitive to ascofuranone, a specific inhibitor of trypanosome AOX.</description><subject>Alternative oxidase</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Ascofuranone</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>Cryptosporidiosis - drug therapy</subject><subject>Cryptosporidium parvum</subject><subject>Cryptosporidium parvum - drug effects</subject><subject>Cryptosporidium parvum - enzymology</subject><subject>Cryptosporidium parvum - genetics</subject><subject>DNA, Protozoan - genetics</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Humans</subject><subject>Mitochondria - enzymology</subject><subject>Molecular Sequence Data</subject><subject>Oxidoreductases - antagonists & inhibitors</subject><subject>Oxidoreductases - chemistry</subject><subject>Oxidoreductases - genetics</subject><subject>Oxidoreductases - metabolism</subject><subject>Phylogenetic analysis</subject><subject>Phylogeny</subject><subject>Recombinant Proteins - antagonists & inhibitors</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sesquiterpenes - pharmacology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhiMEokvhBTggnxAcsoxjx04QF7S0gFSJC0jcLMeeUK8SO7WTVfeJeE2c7krc4DAajef__5H8FcVLClsKVLzbb7summ0FwLa02gJrHhUbCi2UFQX-uNgAgCirlv68KJ6ltAeglIv2aXFBuQQheLMpfn9yEc1M8OAseoOkD5GYo_Z5LJ1P6JOb3QHJ3eJ8GEi4d1YnJG_0MGP0-mF3fnxLnCd6ciaM04D32pNJR539SHbxOM0hTSE665ZxXRyW8T2Zbo9D-IUeZ2eI9pbMtxj1hMs6uxzjTD4RfHpePOn1kPDFuV8WP66vvu--lDffPn_dfbwpDWvYXLJOcsBWcikNlXUuK3vedjX2QjLTIGdaWNoJW9WdEXWjJfC2akXf1LzTml0Wr0-5Uwx3C6ZZjS4ZHAbtMSxJNQANo7L5r5DKlnNgMgurk9DEkFLEXk3RjToeFQW1clR7tXJUK0dFK5U5ZtOrc_rSjWj_Ws7gsuDDSYD5Mw4Oo0rGrQDtA09lg_tX_h-TirPd</recordid><startdate>20040123</startdate><enddate>20040123</enddate><creator>Suzuki, Takashi</creator><creator>Hashimoto, Tetsuo</creator><creator>Yabu, Yoshisada</creator><creator>Kido, Yasutoshi</creator><creator>Sakamoto, Kimitoshi</creator><creator>Nihei, Coh-ichi</creator><creator>Hato, Mariko</creator><creator>Suzuki, Shu-ichi</creator><creator>Amano, Yuko</creator><creator>Nagai, Kazuo</creator><creator>Hosokawa, Tomoyoshi</creator><creator>Minagawa, Nobuko</creator><creator>Ohta, Nobuo</creator><creator>Kita, Kiyoshi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20040123</creationdate><title>Direct evidence for cyanide-insensitive quinol oxidase (alternative oxidase) in apicomplexan parasite Cryptosporidium parvum: phylogenetic and therapeutic implications</title><author>Suzuki, Takashi ; Hashimoto, Tetsuo ; Yabu, Yoshisada ; Kido, Yasutoshi ; Sakamoto, Kimitoshi ; Nihei, Coh-ichi ; Hato, Mariko ; Suzuki, Shu-ichi ; Amano, Yuko ; Nagai, Kazuo ; Hosokawa, Tomoyoshi ; Minagawa, Nobuko ; Ohta, Nobuo ; Kita, Kiyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-3b740e97477c175c17d7f49b5ef673c8e43a6d1b6d25bc658a7049296f854baa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Alternative oxidase</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Ascofuranone</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>Cryptosporidiosis - drug therapy</topic><topic>Cryptosporidium parvum</topic><topic>Cryptosporidium parvum - drug effects</topic><topic>Cryptosporidium parvum - enzymology</topic><topic>Cryptosporidium parvum - genetics</topic><topic>DNA, Protozoan - genetics</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Humans</topic><topic>Mitochondria - enzymology</topic><topic>Molecular Sequence Data</topic><topic>Oxidoreductases - antagonists & inhibitors</topic><topic>Oxidoreductases - chemistry</topic><topic>Oxidoreductases - genetics</topic><topic>Oxidoreductases - metabolism</topic><topic>Phylogenetic analysis</topic><topic>Phylogeny</topic><topic>Recombinant Proteins - antagonists & inhibitors</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sesquiterpenes - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suzuki, Takashi</creatorcontrib><creatorcontrib>Hashimoto, Tetsuo</creatorcontrib><creatorcontrib>Yabu, Yoshisada</creatorcontrib><creatorcontrib>Kido, Yasutoshi</creatorcontrib><creatorcontrib>Sakamoto, Kimitoshi</creatorcontrib><creatorcontrib>Nihei, Coh-ichi</creatorcontrib><creatorcontrib>Hato, Mariko</creatorcontrib><creatorcontrib>Suzuki, Shu-ichi</creatorcontrib><creatorcontrib>Amano, Yuko</creatorcontrib><creatorcontrib>Nagai, Kazuo</creatorcontrib><creatorcontrib>Hosokawa, Tomoyoshi</creatorcontrib><creatorcontrib>Minagawa, Nobuko</creatorcontrib><creatorcontrib>Ohta, Nobuo</creatorcontrib><creatorcontrib>Kita, Kiyoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suzuki, Takashi</au><au>Hashimoto, Tetsuo</au><au>Yabu, Yoshisada</au><au>Kido, Yasutoshi</au><au>Sakamoto, Kimitoshi</au><au>Nihei, Coh-ichi</au><au>Hato, Mariko</au><au>Suzuki, Shu-ichi</au><au>Amano, Yuko</au><au>Nagai, Kazuo</au><au>Hosokawa, Tomoyoshi</au><au>Minagawa, Nobuko</au><au>Ohta, Nobuo</au><au>Kita, Kiyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct evidence for cyanide-insensitive quinol oxidase (alternative oxidase) in apicomplexan parasite Cryptosporidium parvum: phylogenetic and therapeutic implications</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2004-01-23</date><risdate>2004</risdate><volume>313</volume><issue>4</issue><spage>1044</spage><epage>1052</epage><pages>1044-1052</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Cryptosporidium parvum is a parasitic protozoan that causes the diarrheal disease cryptosporidiosis, for which no satisfactory chemotherapy is currently available. Although the presence of mitochondria in this parasite has been suggested, its respiratory system is poorly understood due to difficulties in performing biochemical analyses. In order to better understand the respiratory chain of
C. parvum, we surveyed its genomic DNA database in GenBank and identified a partial sequence encoding cyanide-insensitive alternative oxidase (AOX). Based on this sequence, we cloned
C. parvum AOX (
CpAOX) cDNA from the phylum apicomplexa for the first time. The deduced amino acid sequence (335 a.a.) of CpAOX contains diiron coordination motifs (–E–, –EXXH–) that are conserved among AOXs. Phylogenetic analysis suggested that CpAOX is a mitochondrial-type AOX, possibly derived from mitochondrial endosymbiont gene transfer. The recombinant enzyme expressed in
Escherichia coli showed quinol oxidase activity. This activity was insensitive to cyanide and highly sensitive to ascofuranone, a specific inhibitor of trypanosome AOX.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>14706648</pmid><doi>10.1016/j.bbrc.2003.12.038</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2004-01, Vol.313 (4), p.1044-1052 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80083178 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Alternative oxidase Amino Acid Sequence Animals Ascofuranone Base Sequence Cloning, Molecular Cryptosporidiosis - drug therapy Cryptosporidium parvum Cryptosporidium parvum - drug effects Cryptosporidium parvum - enzymology Cryptosporidium parvum - genetics DNA, Protozoan - genetics Enzyme Inhibitors - pharmacology Humans Mitochondria - enzymology Molecular Sequence Data Oxidoreductases - antagonists & inhibitors Oxidoreductases - chemistry Oxidoreductases - genetics Oxidoreductases - metabolism Phylogenetic analysis Phylogeny Recombinant Proteins - antagonists & inhibitors Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Sequence Homology, Amino Acid Sesquiterpenes - pharmacology |
| title | Direct evidence for cyanide-insensitive quinol oxidase (alternative oxidase) in apicomplexan parasite Cryptosporidium parvum: phylogenetic and therapeutic implications |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T17%3A13%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Direct%20evidence%20for%20cyanide-insensitive%20quinol%20oxidase%20(alternative%20oxidase)%20in%20apicomplexan%20parasite%20Cryptosporidium%20parvum:%20phylogenetic%20and%20therapeutic%20implications&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Suzuki,%20Takashi&rft.date=2004-01-23&rft.volume=313&rft.issue=4&rft.spage=1044&rft.epage=1052&rft.pages=1044-1052&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/j.bbrc.2003.12.038&rft_dat=%3Cproquest_cross%3E80083178%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17944037&rft_id=info:pmid/14706648&rft_els_id=S0006291X03026214&rfr_iscdi=true |