Guideline to reference gene selection for quantitative real-time PCR

Today, quantitative real-time PCR is the method of choice for rapid and reliable quantification of mRNA transcription. However, for an exact comparison of mRNA transcription in different samples or tissues it is crucial to choose the appropriate reference gene. Recently glyceraldehyde 3-phosphate de...

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Veröffentlicht in:	Biochemical and biophysical research communications 2004-01, Vol.313 (4), p.856-862
Hauptverfasser:	Radonić, Aleksandar, Thulke, Stefanie, Mackay, Ian M, Landt, Olfert, Siegert, Wolfgang, Nitsche, Andreas
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Cell Line DNA Primers - genetics DNA Probes - genetics Housekeeping genes Humans Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards Quantitative real-time RCR Reference genes RNA polymerase II RNA Stability RNA, Messenger - genetics Transcription analysis Transcription, Genetic
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