A polymorphic tandem repeat potentially useful for typing in the chromosome of Yersinia enterocolitica
1 Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Avenida de Valdecilla s/n, 39008-Santander, Spain 2 Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Centro Asociado al CIB, CSIC, Cardenal Herrera Oria s/n, 39011, Santander, Spain Correspo...
Gespeichert in:
| Veröffentlicht in: | Microbiology (Society for General Microbiology) 2004-01, Vol.150 (1), p.199-204 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 204 |
|---|---|
| container_issue | 1 |
| container_start_page | 199 |
| container_title | Microbiology (Society for General Microbiology) |
| container_volume | 150 |
| creator | de Benito, Ines Cano, Maria Eliecer Aguero, Jesus Lobo, Juan M. Garcia |
| description | 1 Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Avenida de Valdecilla s/n, 39008-Santander, Spain
2 Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Centro Asociado al CIB, CSIC, Cardenal Herrera Oria s/n, 39011, Santander, Spain
Correspondence Juan M. García Lobo jmglobo{at}unican.es
The hexanucleotide CCAGCA was found repeated 15 times in tandem on the 5' side of the virginiamycin acetyl transferase gene of Yersinia enterocolitica strain Y56. The corresponding region was analysed by PCR from 54 clinical strains belonging to the same biotype and serotype, and others from this laboratory collection belonging to different biotypes and serotypes. Each strain produced a single amplification product whose size was variable among strains, revealing that the locus was polymorphic. Nucleotide sequence determination of selected PCR products showed that the polymorphism was due to the precise expansion or reduction in the number of hexanucleotide repeats. Analysis of this locus in a few strains showing the same PFGE pattern showed that it was also polymorphic. These results suggest that this method could be valuable to increase the discriminatory power of current Y. enterocolitica typing schemes.
Abbreviations: PA, proline-alanine; VNTR, variable number tandem repeats |
| doi_str_mv | 10.1099/mic.0.26642-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80078284</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>80078284</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-67920fa64e091983a7df0b8763d707a86cfee8df7e95d874e43a95910c54159d3</originalsourceid><addsrcrecordid>eNqF0b1v1DAUAHALUdFSGFmRFyqWHM-JHdtjVVFAqsRSBibL5zxfjJw42InQ_fe43EkdmWzp_d6H3iPkHYMdA60_TcHtYNf2PW8beEGuGO9F04KCl_XfCWhAyfaSvC7lF0ANAntFLhmX0HLWXRF_S5cUj1PKyxgcXe084EQzLmjXGllxXoON8Ui3gn6L1KdM1-MS5gMNM11HpG7MaUolTUiTpz8xlzAHS2si5uRSDGtw9g258DYWfHt-r8mP-8-Pd1-bh-9fvt3dPjSOt-3a9FK34G3PETTTqrNy8LBXsu8GCdKq3nlENXiJWgxKcuSd1UIzcIIzoYfumtyc6i45_d6wrGYKxWGMdsa0FaMApGoV_y9kCgTvO11hc4Iup1IyerPkMNl8NAzM0wVqojNg_l3AQPXvz4W3_YTDsz6vvIIPZ2CLs9FnO7tQnp0QkoMU1X08uTEcxj8hozngXHvltA_pqSkTdQDDtO7-Av6LnY4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18054639</pqid></control><display><type>article</type><title>A polymorphic tandem repeat potentially useful for typing in the chromosome of Yersinia enterocolitica</title><source>MEDLINE</source><source>PubMed Central</source><creator>de Benito, Ines ; Cano, Maria Eliecer ; Aguero, Jesus ; Lobo, Juan M. Garcia</creator><creatorcontrib>de Benito, Ines ; Cano, Maria Eliecer ; Aguero, Jesus ; Lobo, Juan M. Garcia</creatorcontrib><description>1 Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Avenida de Valdecilla s/n, 39008-Santander, Spain
2 Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Centro Asociado al CIB, CSIC, Cardenal Herrera Oria s/n, 39011, Santander, Spain
Correspondence Juan M. García Lobo jmglobo{at}unican.es
The hexanucleotide CCAGCA was found repeated 15 times in tandem on the 5' side of the virginiamycin acetyl transferase gene of Yersinia enterocolitica strain Y56. The corresponding region was analysed by PCR from 54 clinical strains belonging to the same biotype and serotype, and others from this laboratory collection belonging to different biotypes and serotypes. Each strain produced a single amplification product whose size was variable among strains, revealing that the locus was polymorphic. Nucleotide sequence determination of selected PCR products showed that the polymorphism was due to the precise expansion or reduction in the number of hexanucleotide repeats. Analysis of this locus in a few strains showing the same PFGE pattern showed that it was also polymorphic. These results suggest that this method could be valuable to increase the discriminatory power of current Y. enterocolitica typing schemes.
Abbreviations: PA, proline-alanine; VNTR, variable number tandem repeats</description><identifier>ISSN: 1350-0872</identifier><identifier>EISSN: 1465-2080</identifier><identifier>DOI: 10.1099/mic.0.26642-0</identifier><identifier>PMID: 14702413</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Amino Acid Sequence ; Bacterial Typing Techniques - methods ; Bacteriology ; Base Sequence ; Biological and medical sciences ; DNA, Bacterial - genetics ; Electrophoresis, Gel, Pulsed-Field ; Fundamental and applied biological sciences. Psychology ; Humans ; Microbiology ; Minisatellite Repeats ; Miscellaneous ; Molecular Sequence Data ; Open Reading Frames ; Polymorphism, Genetic ; Sequence Homology, Amino Acid ; Yersinia - classification ; Yersinia - genetics ; Yersinia enterocolitica ; Yersinia enterocolitica - classification ; Yersinia enterocolitica - genetics ; Yersinia enterocolitica - isolation & purification</subject><ispartof>Microbiology (Society for General Microbiology), 2004-01, Vol.150 (1), p.199-204</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-67920fa64e091983a7df0b8763d707a86cfee8df7e95d874e43a95910c54159d3</citedby><cites>FETCH-LOGICAL-c422t-67920fa64e091983a7df0b8763d707a86cfee8df7e95d874e43a95910c54159d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15574075$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14702413$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Benito, Ines</creatorcontrib><creatorcontrib>Cano, Maria Eliecer</creatorcontrib><creatorcontrib>Aguero, Jesus</creatorcontrib><creatorcontrib>Lobo, Juan M. Garcia</creatorcontrib><title>A polymorphic tandem repeat potentially useful for typing in the chromosome of Yersinia enterocolitica</title><title>Microbiology (Society for General Microbiology)</title><addtitle>Microbiology</addtitle><description>1 Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Avenida de Valdecilla s/n, 39008-Santander, Spain
2 Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Centro Asociado al CIB, CSIC, Cardenal Herrera Oria s/n, 39011, Santander, Spain
Correspondence Juan M. García Lobo jmglobo{at}unican.es
The hexanucleotide CCAGCA was found repeated 15 times in tandem on the 5' side of the virginiamycin acetyl transferase gene of Yersinia enterocolitica strain Y56. The corresponding region was analysed by PCR from 54 clinical strains belonging to the same biotype and serotype, and others from this laboratory collection belonging to different biotypes and serotypes. Each strain produced a single amplification product whose size was variable among strains, revealing that the locus was polymorphic. Nucleotide sequence determination of selected PCR products showed that the polymorphism was due to the precise expansion or reduction in the number of hexanucleotide repeats. Analysis of this locus in a few strains showing the same PFGE pattern showed that it was also polymorphic. These results suggest that this method could be valuable to increase the discriminatory power of current Y. enterocolitica typing schemes.
Abbreviations: PA, proline-alanine; VNTR, variable number tandem repeats</description><subject>Amino Acid Sequence</subject><subject>Bacterial Typing Techniques - methods</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA, Bacterial - genetics</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Minisatellite Repeats</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Open Reading Frames</subject><subject>Polymorphism, Genetic</subject><subject>Sequence Homology, Amino Acid</subject><subject>Yersinia - classification</subject><subject>Yersinia - genetics</subject><subject>Yersinia enterocolitica</subject><subject>Yersinia enterocolitica - classification</subject><subject>Yersinia enterocolitica - genetics</subject><subject>Yersinia enterocolitica - isolation & purification</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0b1v1DAUAHALUdFSGFmRFyqWHM-JHdtjVVFAqsRSBibL5zxfjJw42InQ_fe43EkdmWzp_d6H3iPkHYMdA60_TcHtYNf2PW8beEGuGO9F04KCl_XfCWhAyfaSvC7lF0ANAntFLhmX0HLWXRF_S5cUj1PKyxgcXe084EQzLmjXGllxXoON8Ui3gn6L1KdM1-MS5gMNM11HpG7MaUolTUiTpz8xlzAHS2si5uRSDGtw9g258DYWfHt-r8mP-8-Pd1-bh-9fvt3dPjSOt-3a9FK34G3PETTTqrNy8LBXsu8GCdKq3nlENXiJWgxKcuSd1UIzcIIzoYfumtyc6i45_d6wrGYKxWGMdsa0FaMApGoV_y9kCgTvO11hc4Iup1IyerPkMNl8NAzM0wVqojNg_l3AQPXvz4W3_YTDsz6vvIIPZ2CLs9FnO7tQnp0QkoMU1X08uTEcxj8hozngXHvltA_pqSkTdQDDtO7-Av6LnY4</recordid><startdate>20040101</startdate><enddate>20040101</enddate><creator>de Benito, Ines</creator><creator>Cano, Maria Eliecer</creator><creator>Aguero, Jesus</creator><creator>Lobo, Juan M. Garcia</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20040101</creationdate><title>A polymorphic tandem repeat potentially useful for typing in the chromosome of Yersinia enterocolitica</title><author>de Benito, Ines ; Cano, Maria Eliecer ; Aguero, Jesus ; Lobo, Juan M. Garcia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-67920fa64e091983a7df0b8763d707a86cfee8df7e95d874e43a95910c54159d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>Bacterial Typing Techniques - methods</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA, Bacterial - genetics</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Minisatellite Repeats</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Open Reading Frames</topic><topic>Polymorphism, Genetic</topic><topic>Sequence Homology, Amino Acid</topic><topic>Yersinia - classification</topic><topic>Yersinia - genetics</topic><topic>Yersinia enterocolitica</topic><topic>Yersinia enterocolitica - classification</topic><topic>Yersinia enterocolitica - genetics</topic><topic>Yersinia enterocolitica - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Benito, Ines</creatorcontrib><creatorcontrib>Cano, Maria Eliecer</creatorcontrib><creatorcontrib>Aguero, Jesus</creatorcontrib><creatorcontrib>Lobo, Juan M. Garcia</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Benito, Ines</au><au>Cano, Maria Eliecer</au><au>Aguero, Jesus</au><au>Lobo, Juan M. Garcia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A polymorphic tandem repeat potentially useful for typing in the chromosome of Yersinia enterocolitica</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>2004-01-01</date><risdate>2004</risdate><volume>150</volume><issue>1</issue><spage>199</spage><epage>204</epage><pages>199-204</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>1 Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Avenida de Valdecilla s/n, 39008-Santander, Spain
2 Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Centro Asociado al CIB, CSIC, Cardenal Herrera Oria s/n, 39011, Santander, Spain
Correspondence Juan M. García Lobo jmglobo{at}unican.es
The hexanucleotide CCAGCA was found repeated 15 times in tandem on the 5' side of the virginiamycin acetyl transferase gene of Yersinia enterocolitica strain Y56. The corresponding region was analysed by PCR from 54 clinical strains belonging to the same biotype and serotype, and others from this laboratory collection belonging to different biotypes and serotypes. Each strain produced a single amplification product whose size was variable among strains, revealing that the locus was polymorphic. Nucleotide sequence determination of selected PCR products showed that the polymorphism was due to the precise expansion or reduction in the number of hexanucleotide repeats. Analysis of this locus in a few strains showing the same PFGE pattern showed that it was also polymorphic. These results suggest that this method could be valuable to increase the discriminatory power of current Y. enterocolitica typing schemes.
Abbreviations: PA, proline-alanine; VNTR, variable number tandem repeats</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>14702413</pmid><doi>10.1099/mic.0.26642-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1350-0872 |
| ispartof | Microbiology (Society for General Microbiology), 2004-01, Vol.150 (1), p.199-204 |
| issn | 1350-0872 1465-2080 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80078284 |
| source | MEDLINE; PubMed Central |
| subjects | Amino Acid Sequence Bacterial Typing Techniques - methods Bacteriology Base Sequence Biological and medical sciences DNA, Bacterial - genetics Electrophoresis, Gel, Pulsed-Field Fundamental and applied biological sciences. Psychology Humans Microbiology Minisatellite Repeats Miscellaneous Molecular Sequence Data Open Reading Frames Polymorphism, Genetic Sequence Homology, Amino Acid Yersinia - classification Yersinia - genetics Yersinia enterocolitica Yersinia enterocolitica - classification Yersinia enterocolitica - genetics Yersinia enterocolitica - isolation & purification |
| title | A polymorphic tandem repeat potentially useful for typing in the chromosome of Yersinia enterocolitica |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T00%3A24%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20polymorphic%20tandem%20repeat%20potentially%20useful%20for%20typing%20in%20the%20chromosome%20of%20Yersinia%20enterocolitica&rft.jtitle=Microbiology%20(Society%20for%20General%20Microbiology)&rft.au=de%20Benito,%20Ines&rft.date=2004-01-01&rft.volume=150&rft.issue=1&rft.spage=199&rft.epage=204&rft.pages=199-204&rft.issn=1350-0872&rft.eissn=1465-2080&rft_id=info:doi/10.1099/mic.0.26642-0&rft_dat=%3Cproquest_cross%3E80078284%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18054639&rft_id=info:pmid/14702413&rfr_iscdi=true |