Content and contractile effect of arginine vasopressin in rat urinary bladder
The contractile response of normal male rat urinary bladders to exogenous arginine vasopressin (AVP) and the AVP content of normal and denervated bladders were investigated, In isolated detrusor strips, the maximal response to AVP was about 12% of the contraction elicited by KC1 (124 mM), and the EC...
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| Veröffentlicht in: | European journal of pharmacology 1990-07, Vol.182 (3), p.549-554 |
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| creator | Uvelius, Bengt Lundin, Stefan Andersson, Karl-Erik |
| description | The contractile response of normal male rat urinary bladders to exogenous arginine vasopressin (AVP) and the AVP content of normal and denervated bladders were investigated, In isolated detrusor strips, the maximal response to AVP was about 12% of the contraction elicited by KC1 (124 mM), and the EC
50 value was 1.03 ± 0.13 × 10
−8 M. The response to transmural nerve stimulation was not affected by the presence of AVP. Addition of an AVP receptor antagonist strongly reduced the response to exogenous AVP, but did not affect contractions in response to nerve stimulation. In normal bladders, the concentration of immunoreactive (ir) AVP was 29 ± 6.0 × 10
−15 mol/g. Three days after denervation the bladders had increased 2.4-fold in weight. At this time, the concentration of irAVP was not different from the control value, but the total content had increased significantly. Characterization of bladder irAVP by reverse-phase HPLC revealed that 66.5% of the total immunoreactivity eluted in the position of synthetic AVP. The results suggest a non-neuronal localization of bladder irAVP. |
| doi_str_mv | 10.1016/0014-2999(90)90054-A |
| format | Article |
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50 value was 1.03 ± 0.13 × 10
−8 M. The response to transmural nerve stimulation was not affected by the presence of AVP. Addition of an AVP receptor antagonist strongly reduced the response to exogenous AVP, but did not affect contractions in response to nerve stimulation. In normal bladders, the concentration of immunoreactive (ir) AVP was 29 ± 6.0 × 10
−15 mol/g. Three days after denervation the bladders had increased 2.4-fold in weight. At this time, the concentration of irAVP was not different from the control value, but the total content had increased significantly. Characterization of bladder irAVP by reverse-phase HPLC revealed that 66.5% of the total immunoreactivity eluted in the position of synthetic AVP. The results suggest a non-neuronal localization of bladder irAVP.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/0014-2999(90)90054-A</identifier><identifier>PMID: 2226623</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Arginine Vasopressin - analogs & derivatives ; Arginine Vasopressin - metabolism ; Arginine Vasopressin - pharmacology ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; Denervation ; Electric Stimulation ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Male ; Muscle Contraction - drug effects ; Muscle Denervation ; Muscle, Smooth - drug effects ; Muscle, Smooth - metabolism ; Radioimmunoassay ; Rat ; Rats ; Rats, Inbred Strains ; Urinary bladder ; Urinary Bladder - drug effects ; Urinary Bladder - metabolism ; Vasopressin ; Vertebrates: urinary system</subject><ispartof>European journal of pharmacology, 1990-07, Vol.182 (3), p.549-554</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-9511946ba6abffab005662681f3ecdcaa24e49ca57eddeb42d05aa86b1f71db63</citedby><cites>FETCH-LOGICAL-c438t-9511946ba6abffab005662681f3ecdcaa24e49ca57eddeb42d05aa86b1f71db63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/001429999090054A$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19328983$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2226623$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Uvelius, Bengt</creatorcontrib><creatorcontrib>Lundin, Stefan</creatorcontrib><creatorcontrib>Andersson, Karl-Erik</creatorcontrib><title>Content and contractile effect of arginine vasopressin in rat urinary bladder</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>The contractile response of normal male rat urinary bladders to exogenous arginine vasopressin (AVP) and the AVP content of normal and denervated bladders were investigated, In isolated detrusor strips, the maximal response to AVP was about 12% of the contraction elicited by KC1 (124 mM), and the EC
50 value was 1.03 ± 0.13 × 10
−8 M. The response to transmural nerve stimulation was not affected by the presence of AVP. Addition of an AVP receptor antagonist strongly reduced the response to exogenous AVP, but did not affect contractions in response to nerve stimulation. In normal bladders, the concentration of immunoreactive (ir) AVP was 29 ± 6.0 × 10
−15 mol/g. Three days after denervation the bladders had increased 2.4-fold in weight. At this time, the concentration of irAVP was not different from the control value, but the total content had increased significantly. Characterization of bladder irAVP by reverse-phase HPLC revealed that 66.5% of the total immunoreactivity eluted in the position of synthetic AVP. The results suggest a non-neuronal localization of bladder irAVP.</description><subject>Animals</subject><subject>Arginine Vasopressin - analogs & derivatives</subject><subject>Arginine Vasopressin - metabolism</subject><subject>Arginine Vasopressin - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Denervation</subject><subject>Electric Stimulation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle Denervation</subject><subject>Muscle, Smooth - drug effects</subject><subject>Muscle, Smooth - metabolism</subject><subject>Radioimmunoassay</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Urinary bladder</subject><subject>Urinary Bladder - drug effects</subject><subject>Urinary Bladder - metabolism</subject><subject>Vasopressin</subject><subject>Vertebrates: urinary system</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFqGzEQhkVpcFw3b9CCLgnpYVNJq9WuLgFj0ibg0EtzFrPSqKistY60NuTtI9cmuRUEI5hvfn4-Qr5wdsMZV98Z47ISWutrzb5pxhpZLT-QOe9aXbGWi49k_oack085_2UF0qKZkZkQQilRz8njaowTxolCdNSWfwI7hQEpeo92oqOnkP6EGCLSPeRxmzDnEGl5CSa6SyFCeqH9AM5h-kzOPAwZL05zQZ5-3P1e3VfrXz8fVst1ZWXdTZVuONdS9aCg9x76Uqu0UR33NVpnAYREqS00LZbUXgrHGoBO9dy33PWqXpCrY-42jc87zJPZhGxxGCDiuMumY6xtVSsLKI-gTWPOCb3ZprApjQ1n5mDRHBSZgyKjmfln0SzL2ddT_q7foHs7Omkr-8vTHrKFwSeINuT3bF2LTncH7vbIYZGxD5hMtgGjRRdSsWvcGP5f5BU-4Y8x</recordid><startdate>19900717</startdate><enddate>19900717</enddate><creator>Uvelius, Bengt</creator><creator>Lundin, Stefan</creator><creator>Andersson, Karl-Erik</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900717</creationdate><title>Content and contractile effect of arginine vasopressin in rat urinary bladder</title><author>Uvelius, Bengt ; Lundin, Stefan ; Andersson, Karl-Erik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-9511946ba6abffab005662681f3ecdcaa24e49ca57eddeb42d05aa86b1f71db63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Arginine Vasopressin - analogs & derivatives</topic><topic>Arginine Vasopressin - metabolism</topic><topic>Arginine Vasopressin - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Denervation</topic><topic>Electric Stimulation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle Denervation</topic><topic>Muscle, Smooth - drug effects</topic><topic>Muscle, Smooth - metabolism</topic><topic>Radioimmunoassay</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Urinary bladder</topic><topic>Urinary Bladder - drug effects</topic><topic>Urinary Bladder - metabolism</topic><topic>Vasopressin</topic><topic>Vertebrates: urinary system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Uvelius, Bengt</creatorcontrib><creatorcontrib>Lundin, Stefan</creatorcontrib><creatorcontrib>Andersson, Karl-Erik</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Uvelius, Bengt</au><au>Lundin, Stefan</au><au>Andersson, Karl-Erik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Content and contractile effect of arginine vasopressin in rat urinary bladder</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1990-07-17</date><risdate>1990</risdate><volume>182</volume><issue>3</issue><spage>549</spage><epage>554</epage><pages>549-554</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>The contractile response of normal male rat urinary bladders to exogenous arginine vasopressin (AVP) and the AVP content of normal and denervated bladders were investigated, In isolated detrusor strips, the maximal response to AVP was about 12% of the contraction elicited by KC1 (124 mM), and the EC
50 value was 1.03 ± 0.13 × 10
−8 M. The response to transmural nerve stimulation was not affected by the presence of AVP. Addition of an AVP receptor antagonist strongly reduced the response to exogenous AVP, but did not affect contractions in response to nerve stimulation. In normal bladders, the concentration of immunoreactive (ir) AVP was 29 ± 6.0 × 10
−15 mol/g. Three days after denervation the bladders had increased 2.4-fold in weight. At this time, the concentration of irAVP was not different from the control value, but the total content had increased significantly. Characterization of bladder irAVP by reverse-phase HPLC revealed that 66.5% of the total immunoreactivity eluted in the position of synthetic AVP. The results suggest a non-neuronal localization of bladder irAVP.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2226623</pmid><doi>10.1016/0014-2999(90)90054-A</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Arginine Vasopressin - analogs & derivatives Arginine Vasopressin - metabolism Arginine Vasopressin - pharmacology Biological and medical sciences Chromatography, High Pressure Liquid Denervation Electric Stimulation Fundamental and applied biological sciences. Psychology In Vitro Techniques Male Muscle Contraction - drug effects Muscle Denervation Muscle, Smooth - drug effects Muscle, Smooth - metabolism Radioimmunoassay Rat Rats Rats, Inbred Strains Urinary bladder Urinary Bladder - drug effects Urinary Bladder - metabolism Vasopressin Vertebrates: urinary system |
| title | Content and contractile effect of arginine vasopressin in rat urinary bladder |
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