Olfactory ensheathing cells promote collateral axonal branching in the injured adult rat spinal cord
In recent years, injection of olfactory ensheathing cells (ECs) into the spinal cord has been used as an experimental strategy to promote regeneration of injured axons. In this study, we have compared the effects of transplanting encapsulated ECs with those injected directly into the spinal cord. Th...
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| Veröffentlicht in: | Experimental neurology 2004, Vol.185 (1), p.15-25 |
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| creator | Chuah, M.I. Choi-Lundberg, D. Weston, S. Vincent, A.J. Chung, R.S. Vickers, J.C. West, A.K. |
| description | In recent years, injection of olfactory ensheathing cells (ECs) into the spinal cord has been used as an experimental strategy to promote regeneration of injured axons. In this study, we have compared the effects of transplanting encapsulated ECs with those injected directly into the spinal cord. The dorsal columns of adult rats were cut at T
8–9 and rats in experimental groups received either EC-filled porous polymer capsules or culture medium (CM)-filled capsules with ECs injected at the injury site. Control rats were in three groups: (1) uninjured, (2) lesion with transplantation of CM-filled capsules and (3) lesion with transplantation of CM-filled capsules and injections of CM. Three weeks after injury, Fluororuby was injected into the hindlimb motor and somatosensory cortex to label corticospinal neurons. Observations indicated that there were a few regenerating fibres, up to 10, in the EC-treated groups. In rats that received encapsulated ECs, regenerating fibres were present in close association with the capsule. Rats that received EC injections demonstrated a significant increase in the number of collateral branches from the intact ventral corticospinal tract (vCST) compared with the corresponding control, CM-injected group (
P = 0.003), while a trend for increased collateral branches was observed in rats that received encapsulated ECs (
P = 0.07). |
| doi_str_mv | 10.1016/j.expneurol.2003.09.008 |
| format | Article |
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8–9 and rats in experimental groups received either EC-filled porous polymer capsules or culture medium (CM)-filled capsules with ECs injected at the injury site. Control rats were in three groups: (1) uninjured, (2) lesion with transplantation of CM-filled capsules and (3) lesion with transplantation of CM-filled capsules and injections of CM. Three weeks after injury, Fluororuby was injected into the hindlimb motor and somatosensory cortex to label corticospinal neurons. Observations indicated that there were a few regenerating fibres, up to 10, in the EC-treated groups. In rats that received encapsulated ECs, regenerating fibres were present in close association with the capsule. Rats that received EC injections demonstrated a significant increase in the number of collateral branches from the intact ventral corticospinal tract (vCST) compared with the corresponding control, CM-injected group (
P = 0.003), while a trend for increased collateral branches was observed in rats that received encapsulated ECs (
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8–9 and rats in experimental groups received either EC-filled porous polymer capsules or culture medium (CM)-filled capsules with ECs injected at the injury site. Control rats were in three groups: (1) uninjured, (2) lesion with transplantation of CM-filled capsules and (3) lesion with transplantation of CM-filled capsules and injections of CM. Three weeks after injury, Fluororuby was injected into the hindlimb motor and somatosensory cortex to label corticospinal neurons. Observations indicated that there were a few regenerating fibres, up to 10, in the EC-treated groups. In rats that received encapsulated ECs, regenerating fibres were present in close association with the capsule. Rats that received EC injections demonstrated a significant increase in the number of collateral branches from the intact ventral corticospinal tract (vCST) compared with the corresponding control, CM-injected group (
P = 0.003), while a trend for increased collateral branches was observed in rats that received encapsulated ECs (
P = 0.07).</description><subject>Animals</subject><subject>Axons - physiology</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>Collateral sprouting</subject><subject>Corticospinal tract</subject><subject>Development. Senescence. Regeneration. Transplantation</subject><subject>Dextrans</subject><subject>Disease Models, Animal</subject><subject>Fluorescent Dyes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hindlimb - innervation</subject><subject>Hindlimb - physiopathology</subject><subject>Immunohistochemistry</subject><subject>Injuries of the nervous system and the skull. Diseases due to physical agents</subject><subject>Medical sciences</subject><subject>Nerve Regeneration - physiology</subject><subject>Olfactory ensheathing cells</subject><subject>Olfactory Mucosa - innervation</subject><subject>Olfactory Nerve - cytology</subject><subject>Olfactory Nerve - transplantation</subject><subject>Pyramidal Tracts - cytology</subject><subject>Pyramidal Tracts - injuries</subject><subject>Pyramidal Tracts - physiopathology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Regeneration</subject><subject>Rhodamines</subject><subject>Spinal cord</subject><subject>Spinal Cord Injuries - pathology</subject><subject>Spinal Cord Injuries - therapy</subject><subject>Time Factors</subject><subject>Traumas. Diseases due to physical agents</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0014-4886</issn><issn>1090-2430</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu1DAQhi1URJfCK7S-lFvCOIkT-1hVLSBV6gXOlmOPWa-88WI7qPv2JOyKHjn9h_nG8_sj5IZBzYD1n3c1vhwmnFMMdQPQ1iBrAPGGbBhIqJquhQuyAWBd1QnRX5L3Oe8AQHbN8I5csq6XQ8v4htjn4LQpMR0pTnmLumz99JMaDCHTQ4r7WJCaGIIumHSg-iVOS4xJT-Yv6SdatrjEbk5oqbZzKDTpQvPBr6SJyX4gb50OGT-e84r8eHz4fv-1enr-8u3-7qkynDWlctaAFCAHProGG-F6OUrZWTTcjQ3jfBkyPQjBWtcOoLVowWgueoPQSM7aK_Lp9O5S_NeMuai9z-tX9IRxzkoADD2XKzicQJNizgmdOiS_1-moGKhVsNqpf4LVKliBVIvgZfP6fGIe92hf985GF-D2DOhsdHCrKJ9fOd6Jjg1rhbsTh4uQ3x6TysbjZND6hKYoG_1_y_wBQJyfMw</recordid><startdate>2004</startdate><enddate>2004</enddate><creator>Chuah, M.I.</creator><creator>Choi-Lundberg, D.</creator><creator>Weston, S.</creator><creator>Vincent, A.J.</creator><creator>Chung, R.S.</creator><creator>Vickers, J.C.</creator><creator>West, A.K.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2004</creationdate><title>Olfactory ensheathing cells promote collateral axonal branching in the injured adult rat spinal cord</title><author>Chuah, M.I. ; Choi-Lundberg, D. ; Weston, S. ; Vincent, A.J. ; Chung, R.S. ; Vickers, J.C. ; West, A.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-fdc0980975bf2e28f69b994dec5fb21559801a78813f370aa830ca586ce029513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Axons - physiology</topic><topic>Biological and medical sciences</topic><topic>Cell Count</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Survival</topic><topic>Cells, Cultured</topic><topic>Collateral sprouting</topic><topic>Corticospinal tract</topic><topic>Development. Senescence. Regeneration. Transplantation</topic><topic>Dextrans</topic><topic>Disease Models, Animal</topic><topic>Fluorescent Dyes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hindlimb - innervation</topic><topic>Hindlimb - physiopathology</topic><topic>Immunohistochemistry</topic><topic>Injuries of the nervous system and the skull. Diseases due to physical agents</topic><topic>Medical sciences</topic><topic>Nerve Regeneration - physiology</topic><topic>Olfactory ensheathing cells</topic><topic>Olfactory Mucosa - innervation</topic><topic>Olfactory Nerve - cytology</topic><topic>Olfactory Nerve - transplantation</topic><topic>Pyramidal Tracts - cytology</topic><topic>Pyramidal Tracts - injuries</topic><topic>Pyramidal Tracts - physiopathology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Regeneration</topic><topic>Rhodamines</topic><topic>Spinal cord</topic><topic>Spinal Cord Injuries - pathology</topic><topic>Spinal Cord Injuries - therapy</topic><topic>Time Factors</topic><topic>Traumas. Diseases due to physical agents</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chuah, M.I.</creatorcontrib><creatorcontrib>Choi-Lundberg, D.</creatorcontrib><creatorcontrib>Weston, S.</creatorcontrib><creatorcontrib>Vincent, A.J.</creatorcontrib><creatorcontrib>Chung, R.S.</creatorcontrib><creatorcontrib>Vickers, J.C.</creatorcontrib><creatorcontrib>West, A.K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chuah, M.I.</au><au>Choi-Lundberg, D.</au><au>Weston, S.</au><au>Vincent, A.J.</au><au>Chung, R.S.</au><au>Vickers, J.C.</au><au>West, A.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Olfactory ensheathing cells promote collateral axonal branching in the injured adult rat spinal cord</atitle><jtitle>Experimental neurology</jtitle><addtitle>Exp Neurol</addtitle><date>2004</date><risdate>2004</risdate><volume>185</volume><issue>1</issue><spage>15</spage><epage>25</epage><pages>15-25</pages><issn>0014-4886</issn><eissn>1090-2430</eissn><coden>EXNEAC</coden><abstract>In recent years, injection of olfactory ensheathing cells (ECs) into the spinal cord has been used as an experimental strategy to promote regeneration of injured axons. In this study, we have compared the effects of transplanting encapsulated ECs with those injected directly into the spinal cord. The dorsal columns of adult rats were cut at T
8–9 and rats in experimental groups received either EC-filled porous polymer capsules or culture medium (CM)-filled capsules with ECs injected at the injury site. Control rats were in three groups: (1) uninjured, (2) lesion with transplantation of CM-filled capsules and (3) lesion with transplantation of CM-filled capsules and injections of CM. Three weeks after injury, Fluororuby was injected into the hindlimb motor and somatosensory cortex to label corticospinal neurons. Observations indicated that there were a few regenerating fibres, up to 10, in the EC-treated groups. In rats that received encapsulated ECs, regenerating fibres were present in close association with the capsule. Rats that received EC injections demonstrated a significant increase in the number of collateral branches from the intact ventral corticospinal tract (vCST) compared with the corresponding control, CM-injected group (
P = 0.003), while a trend for increased collateral branches was observed in rats that received encapsulated ECs (
P = 0.07).</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>14697315</pmid><doi>10.1016/j.expneurol.2003.09.008</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Axons - physiology Biological and medical sciences Cell Count Cell Differentiation - physiology Cell Survival Cells, Cultured Collateral sprouting Corticospinal tract Development. Senescence. Regeneration. Transplantation Dextrans Disease Models, Animal Fluorescent Dyes Fundamental and applied biological sciences. Psychology Hindlimb - innervation Hindlimb - physiopathology Immunohistochemistry Injuries of the nervous system and the skull. Diseases due to physical agents Medical sciences Nerve Regeneration - physiology Olfactory ensheathing cells Olfactory Mucosa - innervation Olfactory Nerve - cytology Olfactory Nerve - transplantation Pyramidal Tracts - cytology Pyramidal Tracts - injuries Pyramidal Tracts - physiopathology Rats Rats, Wistar Regeneration Rhodamines Spinal cord Spinal Cord Injuries - pathology Spinal Cord Injuries - therapy Time Factors Traumas. Diseases due to physical agents Vertebrates: nervous system and sense organs |
| title | Olfactory ensheathing cells promote collateral axonal branching in the injured adult rat spinal cord |
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