Effects of soybean Kunitz trypsin inhibitor on the cotton boll weevil (Anthonomus grandis)
The cotton boll weevil, Anthonomus grandis, is an economically important pest of cotton in tropical and subtropical areas of several countries in the Americas, causing severe losses due to their damage in cotton floral buds. Enzymatic assays using gut extracts from larval and adult boll weevil have...
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| Veröffentlicht in: | Phytochemistry (Oxford) 2004, Vol.65 (1), p.81-89 |
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| creator | Franco, O.L Dias, S.C Magalhaes, C.P Monteiro, A.C.S Bloch, C. Jr Melo, F.R Oliveira-Neto, O.B Monnerat, R.G Grossi-de-Sa, M.F |
| description | The cotton boll weevil, Anthonomus grandis, is an economically important pest of cotton in tropical and subtropical areas of several countries in the Americas, causing severe losses due to their damage in cotton floral buds. Enzymatic assays using gut extracts from larval and adult boll weevil have demonstrated the presence of digestive serine proteinase-like activities. Furthermore, in vitro assays showed that soybean Kunitz trypsin inhibitor (SKTI) was able to inhibit these enzymes. Previously, in vivo effects of black-eyed pea trypsin chymotrypsin inhibitor (BTCI) have been demonstrated towards the boll weevil pest. Here, when neonate larvae were reared on an artificial diet containing SKTI at three different concentrations, a reduction of larval weight of up to 64% was observed for highest SKTI concentration 500 micromolar. The presence of SKTI caused an increase in mortality and severe deformities of larvae, pupae and adult insects. This work therefore represents the first observation of a Kunitz trypsin inhibitor active in vivo and in vitro against A. grandis. Bioassays suggested that SKTI could be used as a tool in engineering crop plants, which might exhibit increased resistance against cotton boll weevil. |
| doi_str_mv | 10.1016/j.phytochem.2003.09.010 |
| format | Article |
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Jr ; Melo, F.R ; Oliveira-Neto, O.B ; Monnerat, R.G ; Grossi-de-Sa, M.F</creator><creatorcontrib>Franco, O.L ; Dias, S.C ; Magalhaes, C.P ; Monteiro, A.C.S ; Bloch, C. Jr ; Melo, F.R ; Oliveira-Neto, O.B ; Monnerat, R.G ; Grossi-de-Sa, M.F</creatorcontrib><description>The cotton boll weevil, Anthonomus grandis, is an economically important pest of cotton in tropical and subtropical areas of several countries in the Americas, causing severe losses due to their damage in cotton floral buds. Enzymatic assays using gut extracts from larval and adult boll weevil have demonstrated the presence of digestive serine proteinase-like activities. Furthermore, in vitro assays showed that soybean Kunitz trypsin inhibitor (SKTI) was able to inhibit these enzymes. Previously, in vivo effects of black-eyed pea trypsin chymotrypsin inhibitor (BTCI) have been demonstrated towards the boll weevil pest. Here, when neonate larvae were reared on an artificial diet containing SKTI at three different concentrations, a reduction of larval weight of up to 64% was observed for highest SKTI concentration 500 micromolar. The presence of SKTI caused an increase in mortality and severe deformities of larvae, pupae and adult insects. This work therefore represents the first observation of a Kunitz trypsin inhibitor active in vivo and in vitro against A. grandis. Bioassays suggested that SKTI could be used as a tool in engineering crop plants, which might exhibit increased resistance against cotton boll weevil.</description><identifier>ISSN: 0031-9422</identifier><identifier>EISSN: 1873-3700</identifier><identifier>DOI: 10.1016/j.phytochem.2003.09.010</identifier><identifier>PMID: 14697273</identifier><language>eng</language><publisher>Amsterdam: Elsevier</publisher><subject>Agronomy. Soil science and plant productions ; Animal Feed ; animal morphology ; animal proteins ; Animals ; Anthonomus grandis ; Biological and medical sciences ; bovine pancreatic trypsin ; Cattle ; Coleoptera - drug effects ; Coleoptera - enzymology ; cotton boll weevil ; Digestive System - enzymology ; Dose-Response Relationship, Drug ; enzyme inhibition ; Fundamental and applied biological sciences. Psychology ; Genetics and breeding of economic plants ; Glycine max ; grain crops ; insect pests ; Larva - drug effects ; Larva - growth & development ; midgut ; morphogenesis ; mortality ; Pest animals ; Pest resistance ; phytophagous insects ; plant pests ; plant proteins ; Pupa - drug effects ; Pupa - growth & development ; purification ; Serine Endopeptidases - metabolism ; serine proteinases ; soybean kunitz trypsin inhibitor ; soybeans ; Survival Analysis ; Trypsin - metabolism ; Trypsin Inhibitor, Kunitz Soybean - pharmacology ; trypsin inhibitors ; Trypsin Inhibitors - pharmacology ; Varietal selection. 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Jr</creatorcontrib><creatorcontrib>Melo, F.R</creatorcontrib><creatorcontrib>Oliveira-Neto, O.B</creatorcontrib><creatorcontrib>Monnerat, R.G</creatorcontrib><creatorcontrib>Grossi-de-Sa, M.F</creatorcontrib><title>Effects of soybean Kunitz trypsin inhibitor on the cotton boll weevil (Anthonomus grandis)</title><title>Phytochemistry (Oxford)</title><addtitle>Phytochemistry</addtitle><description>The cotton boll weevil, Anthonomus grandis, is an economically important pest of cotton in tropical and subtropical areas of several countries in the Americas, causing severe losses due to their damage in cotton floral buds. Enzymatic assays using gut extracts from larval and adult boll weevil have demonstrated the presence of digestive serine proteinase-like activities. Furthermore, in vitro assays showed that soybean Kunitz trypsin inhibitor (SKTI) was able to inhibit these enzymes. Previously, in vivo effects of black-eyed pea trypsin chymotrypsin inhibitor (BTCI) have been demonstrated towards the boll weevil pest. Here, when neonate larvae were reared on an artificial diet containing SKTI at three different concentrations, a reduction of larval weight of up to 64% was observed for highest SKTI concentration 500 micromolar. The presence of SKTI caused an increase in mortality and severe deformities of larvae, pupae and adult insects. This work therefore represents the first observation of a Kunitz trypsin inhibitor active in vivo and in vitro against A. grandis. Bioassays suggested that SKTI could be used as a tool in engineering crop plants, which might exhibit increased resistance against cotton boll weevil.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Animal Feed</subject><subject>animal morphology</subject><subject>animal proteins</subject><subject>Animals</subject><subject>Anthonomus grandis</subject><subject>Biological and medical sciences</subject><subject>bovine pancreatic trypsin</subject><subject>Cattle</subject><subject>Coleoptera - drug effects</subject><subject>Coleoptera - enzymology</subject><subject>cotton boll weevil</subject><subject>Digestive System - enzymology</subject><subject>Dose-Response Relationship, Drug</subject><subject>enzyme inhibition</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics and breeding of economic plants</subject><subject>Glycine max</subject><subject>grain crops</subject><subject>insect pests</subject><subject>Larva - drug effects</subject><subject>Larva - growth & development</subject><subject>midgut</subject><subject>morphogenesis</subject><subject>mortality</subject><subject>Pest animals</subject><subject>Pest resistance</subject><subject>phytophagous insects</subject><subject>plant pests</subject><subject>plant proteins</subject><subject>Pupa - drug effects</subject><subject>Pupa - growth & development</subject><subject>purification</subject><subject>Serine Endopeptidases - metabolism</subject><subject>serine proteinases</subject><subject>soybean kunitz trypsin inhibitor</subject><subject>soybeans</subject><subject>Survival Analysis</subject><subject>Trypsin - metabolism</subject><subject>Trypsin Inhibitor, Kunitz Soybean - pharmacology</subject><subject>trypsin inhibitors</subject><subject>Trypsin Inhibitors - pharmacology</subject><subject>Varietal selection. 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Jr</creator><creator>Melo, F.R</creator><creator>Oliveira-Neto, O.B</creator><creator>Monnerat, R.G</creator><creator>Grossi-de-Sa, M.F</creator><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2004</creationdate><title>Effects of soybean Kunitz trypsin inhibitor on the cotton boll weevil (Anthonomus grandis)</title><author>Franco, O.L ; Dias, S.C ; Magalhaes, C.P ; Monteiro, A.C.S ; Bloch, C. Jr ; Melo, F.R ; Oliveira-Neto, O.B ; Monnerat, R.G ; Grossi-de-Sa, M.F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c278t-88f5668e6dc3c4fdeb7084ed4e5aabdce6121f4c5cec96ddecce9c58f914a3f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Animal Feed</topic><topic>animal morphology</topic><topic>animal proteins</topic><topic>Animals</topic><topic>Anthonomus grandis</topic><topic>Biological and medical sciences</topic><topic>bovine pancreatic trypsin</topic><topic>Cattle</topic><topic>Coleoptera - drug effects</topic><topic>Coleoptera - enzymology</topic><topic>cotton boll weevil</topic><topic>Digestive System - enzymology</topic><topic>Dose-Response Relationship, Drug</topic><topic>enzyme inhibition</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics and breeding of economic plants</topic><topic>Glycine max</topic><topic>grain crops</topic><topic>insect pests</topic><topic>Larva - drug effects</topic><topic>Larva - growth & development</topic><topic>midgut</topic><topic>morphogenesis</topic><topic>mortality</topic><topic>Pest animals</topic><topic>Pest resistance</topic><topic>phytophagous insects</topic><topic>plant pests</topic><topic>plant proteins</topic><topic>Pupa - drug effects</topic><topic>Pupa - growth & development</topic><topic>purification</topic><topic>Serine Endopeptidases - metabolism</topic><topic>serine proteinases</topic><topic>soybean kunitz trypsin inhibitor</topic><topic>soybeans</topic><topic>Survival Analysis</topic><topic>Trypsin - metabolism</topic><topic>Trypsin Inhibitor, Kunitz Soybean - pharmacology</topic><topic>trypsin inhibitors</topic><topic>Trypsin Inhibitors - pharmacology</topic><topic>Varietal selection. 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Jr</au><au>Melo, F.R</au><au>Oliveira-Neto, O.B</au><au>Monnerat, R.G</au><au>Grossi-de-Sa, M.F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of soybean Kunitz trypsin inhibitor on the cotton boll weevil (Anthonomus grandis)</atitle><jtitle>Phytochemistry (Oxford)</jtitle><addtitle>Phytochemistry</addtitle><date>2004</date><risdate>2004</risdate><volume>65</volume><issue>1</issue><spage>81</spage><epage>89</epage><pages>81-89</pages><issn>0031-9422</issn><eissn>1873-3700</eissn><abstract>The cotton boll weevil, Anthonomus grandis, is an economically important pest of cotton in tropical and subtropical areas of several countries in the Americas, causing severe losses due to their damage in cotton floral buds. Enzymatic assays using gut extracts from larval and adult boll weevil have demonstrated the presence of digestive serine proteinase-like activities. Furthermore, in vitro assays showed that soybean Kunitz trypsin inhibitor (SKTI) was able to inhibit these enzymes. Previously, in vivo effects of black-eyed pea trypsin chymotrypsin inhibitor (BTCI) have been demonstrated towards the boll weevil pest. Here, when neonate larvae were reared on an artificial diet containing SKTI at three different concentrations, a reduction of larval weight of up to 64% was observed for highest SKTI concentration 500 micromolar. The presence of SKTI caused an increase in mortality and severe deformities of larvae, pupae and adult insects. This work therefore represents the first observation of a Kunitz trypsin inhibitor active in vivo and in vitro against A. grandis. 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| subjects | Agronomy. Soil science and plant productions Animal Feed animal morphology animal proteins Animals Anthonomus grandis Biological and medical sciences bovine pancreatic trypsin Cattle Coleoptera - drug effects Coleoptera - enzymology cotton boll weevil Digestive System - enzymology Dose-Response Relationship, Drug enzyme inhibition Fundamental and applied biological sciences. Psychology Genetics and breeding of economic plants Glycine max grain crops insect pests Larva - drug effects Larva - growth & development midgut morphogenesis mortality Pest animals Pest resistance phytophagous insects plant pests plant proteins Pupa - drug effects Pupa - growth & development purification Serine Endopeptidases - metabolism serine proteinases soybean kunitz trypsin inhibitor soybeans Survival Analysis Trypsin - metabolism Trypsin Inhibitor, Kunitz Soybean - pharmacology trypsin inhibitors Trypsin Inhibitors - pharmacology Varietal selection. Specialized plant breeding, plant breeding aims |
| title | Effects of soybean Kunitz trypsin inhibitor on the cotton boll weevil (Anthonomus grandis) |
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