Bio-fermentation of modified flavonoids: an example of in vivo diversification of secondary metabolites

A bio-fermentation technique was used for the in vivo diversification of flavonoid structures based on expression in Escherichia coli of six O-methyltransferases (OMTs) from Mentha x piperita and one O-glucosyltransferase (GT) each from Arabidopsis thaliana and Allium cepa. Enzymes were shown to be...

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Veröffentlicht in:	Phytochemistry (Oxford) 2004, Vol.65 (1), p.31-41
Hauptverfasser:	Willits, M.G, Giovanni, M, Prata, R.T.N, Kramer, C.M, Luca, V. de, Steffens, J.C, Graser, G
Format:	Artikel
Sprache:	eng
Schlagworte:	Allium cepa Amino Acid Sequence amino acid sequences Arabidopsis - enzymology Arabidopsis thaliana Biological and medical sciences chemical structure Cloning, Molecular Consensus Sequence enzyme substrates Enzymes Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Fermentation flavonoids Flavonoids - chemistry Flavonoids - metabolism Fundamental and applied biological sciences. Psychology glucosyltransferases Glucosyltransferases - chemistry Glucosyltransferases - genetics Glucosyltransferases - metabolism Mentha Mentha piperita - enzymology Mentha piperita nothosubsp. piperita Metabolism methyltransferases Methyltransferases - chemistry Methyltransferases - genetics Methyltransferases - metabolism mint Molecular Sequence Data nucleotide sequences Onions - enzymology Phylogeny Plant physiology and development plant proteins quercetin recombinant proteins Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism secondary metabolites Sequence Alignment sequence analysis Sequence Homology, Amino Acid Stereoisomerism Substrate Specificity
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