Position effect at S. cerevisiae telomeres: Reversible repression of Pol II transcription

S. cerevisiae chromosomes end with the telomeric repeat (TG 1–3) n. When any of four Pol II genes was placed immediately adjacent to the telomeric repeats, expression of the gene was reversibly repressed as demonstrated by phenotype and mRNA analyses. For example, cells bearing a telomere-linked cop...

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Veröffentlicht in:Cell 1990-11, Vol.63 (4), p.751-762
Hauptverfasser: Gottschling, Daniel E., Aparicio, Oscar M., Billington, Barbara L., Zakian, Virginia A.
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container_title Cell
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creator Gottschling, Daniel E.
Aparicio, Oscar M.
Billington, Barbara L.
Zakian, Virginia A.
description S. cerevisiae chromosomes end with the telomeric repeat (TG 1–3) n. When any of four Pol II genes was placed immediately adjacent to the telomeric repeats, expression of the gene was reversibly repressed as demonstrated by phenotype and mRNA analyses. For example, cells bearing a telomere-linked copy of ADE2 produced predominantly red colonies (a phenotype characteristic of ade2 − cells) containing white sectors (characteristic of ADE2 + cells). Repression was due to proximity to the telomere itself since an 81 bp tract of (TG 1–3) n positioned downstream of URA3 when URA3 was ∼20 kb from the end of chromosome VII did not alter expression of the gene. However, this internal tract of (TG 1–3) n could spontaneously become telomeric, in which case expression of the URA3 gene was repressed. These data demonstrate that yeast telomeres exert a position effect on the transcription of nearby genes, an effect that is under epigenetic control
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When any of four Pol II genes was placed immediately adjacent to the telomeric repeats, expression of the gene was reversibly repressed as demonstrated by phenotype and mRNA analyses. For example, cells bearing a telomere-linked copy of ADE2 produced predominantly red colonies (a phenotype characteristic of ade2 − cells) containing white sectors (characteristic of ADE2 + cells). Repression was due to proximity to the telomere itself since an 81 bp tract of (TG 1–3) n positioned downstream of URA3 when URA3 was ∼20 kb from the end of chromosome VII did not alter expression of the gene. However, this internal tract of (TG 1–3) n could spontaneously become telomeric, in which case expression of the URA3 gene was repressed. 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When any of four Pol II genes was placed immediately adjacent to the telomeric repeats, expression of the gene was reversibly repressed as demonstrated by phenotype and mRNA analyses. For example, cells bearing a telomere-linked copy of ADE2 produced predominantly red colonies (a phenotype characteristic of ade2 − cells) containing white sectors (characteristic of ADE2 + cells). Repression was due to proximity to the telomere itself since an 81 bp tract of (TG 1–3) n positioned downstream of URA3 when URA3 was ∼20 kb from the end of chromosome VII did not alter expression of the gene. However, this internal tract of (TG 1–3) n could spontaneously become telomeric, in which case expression of the URA3 gene was repressed. These data demonstrate that yeast telomeres exert a position effect on the transcription of nearby genes, an effect that is under epigenetic control</description><subject>Biological and medical sciences</subject><subject>Chromatin. 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When any of four Pol II genes was placed immediately adjacent to the telomeric repeats, expression of the gene was reversibly repressed as demonstrated by phenotype and mRNA analyses. For example, cells bearing a telomere-linked copy of ADE2 produced predominantly red colonies (a phenotype characteristic of ade2 − cells) containing white sectors (characteristic of ADE2 + cells). Repression was due to proximity to the telomere itself since an 81 bp tract of (TG 1–3) n positioned downstream of URA3 when URA3 was ∼20 kb from the end of chromosome VII did not alter expression of the gene. However, this internal tract of (TG 1–3) n could spontaneously become telomeric, in which case expression of the URA3 gene was repressed. 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source MEDLINE; Elsevier ScienceDirect Journals
subjects Biological and medical sciences
Chromatin. Chromosome
Chromosome Mapping
Chromosomes, Fungal - physiology
DNA Polymerase II - biosynthesis
DNA Polymerase II - genetics
DNA, Fungal - genetics
DNA, Fungal - isolation & purification
Enzyme Repression
Fundamental and applied biological sciences. Psychology
Genes, Fungal
Molecular and cellular biology
Molecular genetics
Plasmids
Restriction Mapping
RNA, Fungal - genetics
RNA, Fungal - isolation & purification
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Transcription, Genetic
Uracil - metabolism
title Position effect at S. cerevisiae telomeres: Reversible repression of Pol II transcription
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