Position effect at S. cerevisiae telomeres: Reversible repression of Pol II transcription
S. cerevisiae chromosomes end with the telomeric repeat (TG 1–3) n. When any of four Pol II genes was placed immediately adjacent to the telomeric repeats, expression of the gene was reversibly repressed as demonstrated by phenotype and mRNA analyses. For example, cells bearing a telomere-linked cop...
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description | S. cerevisiae chromosomes end with the telomeric repeat (TG
1–3)
n. When any of four Pol II genes was placed immediately adjacent to the telomeric repeats, expression of the gene was reversibly repressed as demonstrated by phenotype and mRNA analyses. For example, cells bearing a telomere-linked copy of
ADE2 produced predominantly red colonies (a phenotype characteristic of
ade2
− cells) containing white sectors (characteristic of
ADE2
+ cells). Repression was due to proximity to the telomere itself since an 81 bp tract of (TG
1–3)
n positioned downstream of
URA3 when
URA3 was ∼20 kb from the end of chromosome VII did not alter expression of the gene. However, this internal tract of (TG
1–3)
n could spontaneously become telomeric, in which case expression of the
URA3 gene was repressed. These data demonstrate that yeast telomeres exert a position effect on the transcription of nearby genes, an effect that is under epigenetic control |
doi_str_mv | 10.1016/0092-8674(90)90141-Z |
format | Article |
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1–3)
n. When any of four Pol II genes was placed immediately adjacent to the telomeric repeats, expression of the gene was reversibly repressed as demonstrated by phenotype and mRNA analyses. For example, cells bearing a telomere-linked copy of
ADE2 produced predominantly red colonies (a phenotype characteristic of
ade2
− cells) containing white sectors (characteristic of
ADE2
+ cells). Repression was due to proximity to the telomere itself since an 81 bp tract of (TG
1–3)
n positioned downstream of
URA3 when
URA3 was ∼20 kb from the end of chromosome VII did not alter expression of the gene. However, this internal tract of (TG
1–3)
n could spontaneously become telomeric, in which case expression of the
URA3 gene was repressed. These data demonstrate that yeast telomeres exert a position effect on the transcription of nearby genes, an effect that is under epigenetic control</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/0092-8674(90)90141-Z</identifier><identifier>PMID: 2225075</identifier><identifier>CODEN: CELLB5</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Biological and medical sciences ; Chromatin. Chromosome ; Chromosome Mapping ; Chromosomes, Fungal - physiology ; DNA Polymerase II - biosynthesis ; DNA Polymerase II - genetics ; DNA, Fungal - genetics ; DNA, Fungal - isolation & purification ; Enzyme Repression ; Fundamental and applied biological sciences. Psychology ; Genes, Fungal ; Molecular and cellular biology ; Molecular genetics ; Plasmids ; Restriction Mapping ; RNA, Fungal - genetics ; RNA, Fungal - isolation & purification ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Transcription, Genetic ; Uracil - metabolism</subject><ispartof>Cell, 1990-11, Vol.63 (4), p.751-762</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-8eed671f072f1f72304dbd93ed080b606bdca4b10930a53ded7b1f9569df05b3</citedby><cites>FETCH-LOGICAL-c384t-8eed671f072f1f72304dbd93ed080b606bdca4b10930a53ded7b1f9569df05b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/009286749090141Z$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19398750$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2225075$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gottschling, Daniel E.</creatorcontrib><creatorcontrib>Aparicio, Oscar M.</creatorcontrib><creatorcontrib>Billington, Barbara L.</creatorcontrib><creatorcontrib>Zakian, Virginia A.</creatorcontrib><title>Position effect at S. cerevisiae telomeres: Reversible repression of Pol II transcription</title><title>Cell</title><addtitle>Cell</addtitle><description>S. cerevisiae chromosomes end with the telomeric repeat (TG
1–3)
n. When any of four Pol II genes was placed immediately adjacent to the telomeric repeats, expression of the gene was reversibly repressed as demonstrated by phenotype and mRNA analyses. For example, cells bearing a telomere-linked copy of
ADE2 produced predominantly red colonies (a phenotype characteristic of
ade2
− cells) containing white sectors (characteristic of
ADE2
+ cells). Repression was due to proximity to the telomere itself since an 81 bp tract of (TG
1–3)
n positioned downstream of
URA3 when
URA3 was ∼20 kb from the end of chromosome VII did not alter expression of the gene. However, this internal tract of (TG
1–3)
n could spontaneously become telomeric, in which case expression of the
URA3 gene was repressed. These data demonstrate that yeast telomeres exert a position effect on the transcription of nearby genes, an effect that is under epigenetic control</description><subject>Biological and medical sciences</subject><subject>Chromatin. Chromosome</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Fungal - physiology</subject><subject>DNA Polymerase II - biosynthesis</subject><subject>DNA Polymerase II - genetics</subject><subject>DNA, Fungal - genetics</subject><subject>DNA, Fungal - isolation & purification</subject><subject>Enzyme Repression</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Fungal</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Plasmids</subject><subject>Restriction Mapping</subject><subject>RNA, Fungal - genetics</subject><subject>RNA, Fungal - isolation & purification</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Transcription, Genetic</subject><subject>Uracil - metabolism</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9r3DAQxUVoSbdpvkECujS0B6cj27KkHAIl9M9CoKHNpbkIWRqBgne10XgX-u1rd5f01p7EaN57I_2GsTMBlwJE9wHA1JXuVPvOwHsDohXVwxFbCDCqaoWqX7DFs-QVe030CABaSnnMjuu6lqDkgv28y5TGlNccY0Q_cjfyH5fcY8FdouSQjzjk1VTSFf-OOyyU-gF5wc10RbMxR36XB75c8rG4NfmSNnPgG_YyuoHw9HCesPvPn-5vvla3374sbz7eVr7R7VhpxNApEUHVUURVN9CGPpgGA2joO-j64F3bT79qwMkmYFC9iEZ2JkSQfXPCLvaxm5KftkijXSXyOAxujXlLVgMo6Br4r1BIDUrrWdjuhb5kooLRbkpaufLLCrAzeTtjtTNWa8D-IW8fJtv5IX_brzA8mw6op_7bQ9-Rd0OcWPlEf7NNY7SS8_jrvQ4naLuExZJPuPYYUpkWZENO_37Ib8jTn9Y</recordid><startdate>19901116</startdate><enddate>19901116</enddate><creator>Gottschling, Daniel E.</creator><creator>Aparicio, Oscar M.</creator><creator>Billington, Barbara L.</creator><creator>Zakian, Virginia A.</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19901116</creationdate><title>Position effect at S. cerevisiae telomeres: Reversible repression of Pol II transcription</title><author>Gottschling, Daniel E. ; Aparicio, Oscar M. ; Billington, Barbara L. ; Zakian, Virginia A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-8eed671f072f1f72304dbd93ed080b606bdca4b10930a53ded7b1f9569df05b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Biological and medical sciences</topic><topic>Chromatin. Chromosome</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Fungal - physiology</topic><topic>DNA Polymerase II - biosynthesis</topic><topic>DNA Polymerase II - genetics</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Fungal - isolation & purification</topic><topic>Enzyme Repression</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Fungal</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Plasmids</topic><topic>Restriction Mapping</topic><topic>RNA, Fungal - genetics</topic><topic>RNA, Fungal - isolation & purification</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Transcription, Genetic</topic><topic>Uracil - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gottschling, Daniel E.</creatorcontrib><creatorcontrib>Aparicio, Oscar M.</creatorcontrib><creatorcontrib>Billington, Barbara L.</creatorcontrib><creatorcontrib>Zakian, Virginia A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gottschling, Daniel E.</au><au>Aparicio, Oscar M.</au><au>Billington, Barbara L.</au><au>Zakian, Virginia A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Position effect at S. cerevisiae telomeres: Reversible repression of Pol II transcription</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1990-11-16</date><risdate>1990</risdate><volume>63</volume><issue>4</issue><spage>751</spage><epage>762</epage><pages>751-762</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><coden>CELLB5</coden><abstract>S. cerevisiae chromosomes end with the telomeric repeat (TG
1–3)
n. When any of four Pol II genes was placed immediately adjacent to the telomeric repeats, expression of the gene was reversibly repressed as demonstrated by phenotype and mRNA analyses. For example, cells bearing a telomere-linked copy of
ADE2 produced predominantly red colonies (a phenotype characteristic of
ade2
− cells) containing white sectors (characteristic of
ADE2
+ cells). Repression was due to proximity to the telomere itself since an 81 bp tract of (TG
1–3)
n positioned downstream of
URA3 when
URA3 was ∼20 kb from the end of chromosome VII did not alter expression of the gene. However, this internal tract of (TG
1–3)
n could spontaneously become telomeric, in which case expression of the
URA3 gene was repressed. These data demonstrate that yeast telomeres exert a position effect on the transcription of nearby genes, an effect that is under epigenetic control</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>2225075</pmid><doi>10.1016/0092-8674(90)90141-Z</doi><tpages>12</tpages></addata></record> |
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subjects | Biological and medical sciences Chromatin. Chromosome Chromosome Mapping Chromosomes, Fungal - physiology DNA Polymerase II - biosynthesis DNA Polymerase II - genetics DNA, Fungal - genetics DNA, Fungal - isolation & purification Enzyme Repression Fundamental and applied biological sciences. Psychology Genes, Fungal Molecular and cellular biology Molecular genetics Plasmids Restriction Mapping RNA, Fungal - genetics RNA, Fungal - isolation & purification Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Transcription, Genetic Uracil - metabolism |
title | Position effect at S. cerevisiae telomeres: Reversible repression of Pol II transcription |
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