Improved embryo development with decreased apoptosis in blastomeres after the treatment of cloned bovine embryos with β-mercaptoethanol and hemoglobin
In preliminary experiments, the treatment of donor somatic cells with β‐mercaptoethanol (ME) or hemoglobin (Hb) improved in vitro‐development of bovine cloned embryos. This study was subsequently evaluated whether the exposure to Hb and/or ME during in vitro‐maturation or embryo culture could furthe...
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| Veröffentlicht in: | Molecular reproduction and development 2004-02, Vol.67 (2), p.200-206 |
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| creator | Park, Eul Soon Hwang, Woo Suk Kang, Sung Keun Lee, Byeong Chun Han, Jae Yong Lim, Jeong Mook |
| description | In preliminary experiments, the treatment of donor somatic cells with β‐mercaptoethanol (ME) or hemoglobin (Hb) improved in vitro‐development of bovine cloned embryos. This study was subsequently evaluated whether the exposure to Hb and/or ME during in vitro‐maturation or embryo culture could further promote the development of embryos cloned with ME‐treated donor cells. A prospective, randomized study was conducted and, embryo development, cell number, and apoptosis in blastocysts were monitored. A significant (P |
| doi_str_mv | 10.1002/mrd.10392 |
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This study was subsequently evaluated whether the exposure to Hb and/or ME during in vitro‐maturation or embryo culture could further promote the development of embryos cloned with ME‐treated donor cells. A prospective, randomized study was conducted and, embryo development, cell number, and apoptosis in blastocysts were monitored. A significant (P < 0.05) effect was found after the combined treatment of cloned embryos with Hb (1 μg/ml) and ME (10 μM); the development of morulae (53 vs. 35%) was greatly improved, which resulted in enhanced blastocyst formation (38%). However, cell number and apoptosis in blastocysts were predominantly affected by ME rather than Hb; a significant increase in total cell number of blastomeres (142–154 vs. 123 cells/embryo), inner cell mass (ICM) (39–41 vs. 27), and trophectoderm (TE) (103–114 vs. 98), and the ratio of ICM to TE cell number (0.26–0.27 vs. 0.22) was found. Also, the apoptosis index indicating the ratio of apoptotic cell to normal blastomere number was greatly reduced after ME treatments (0.85 vs. 0.056–0.069). When embryos cloned with ME‐treated cells were cultured in Hb + ME‐containing medium, any of the treatments to recipient oocytes before enucleation did not further promote the development. In conclusion, combined treatment of cloned embryos with Hb + ME not only improved in vitro‐development but also decreased blastomere apoptosis. The use of ME‐treated donor cells and the culture of cloned embryos in Hb + ME‐containing medium yielded the optimal results for promoting the production of blastocysts with improved quality. Mol. Reprod. Dev. 67:200–206, 2004. © 2004 Wiley‐Liss, Inc.</description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/mrd.10392</identifier><identifier>PMID: 14694436</identifier><identifier>CODEN: MREDEE</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; apoptosis ; Apoptosis - drug effects ; Biological and medical sciences ; Blastocyst - cytology ; Blastocyst - drug effects ; Blastomeres - cytology ; Blastomeres - drug effects ; Cattle ; Cloning, Organism ; Embryology: invertebrates and vertebrates. Teratology ; Embryonic Development - drug effects ; Female ; Fundamental and applied biological sciences. Psychology ; hemoglobin ; Hemoglobins - pharmacology ; In Vitro Techniques ; Mercaptoethanol - pharmacology ; Molecular embryology ; Nuclear Transfer Techniques ; preimplantation development ; somatic cell nuclear transfer ; β-mercaptoethanol</subject><ispartof>Molecular reproduction and development, 2004-02, Vol.67 (2), p.200-206</ispartof><rights>Copyright © 2004 Wiley‐Liss, Inc.</rights><rights>Copyright 2004 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3892-15bf0c524b252942050df7b514edb5867369f9bb74d28076dea1ce967fa06d113</citedby><cites>FETCH-LOGICAL-c3892-15bf0c524b252942050df7b514edb5867369f9bb74d28076dea1ce967fa06d113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmrd.10392$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmrd.10392$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15758735$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14694436$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Eul Soon</creatorcontrib><creatorcontrib>Hwang, Woo Suk</creatorcontrib><creatorcontrib>Kang, Sung Keun</creatorcontrib><creatorcontrib>Lee, Byeong Chun</creatorcontrib><creatorcontrib>Han, Jae Yong</creatorcontrib><creatorcontrib>Lim, Jeong Mook</creatorcontrib><title>Improved embryo development with decreased apoptosis in blastomeres after the treatment of cloned bovine embryos with β-mercaptoethanol and hemoglobin</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description>In preliminary experiments, the treatment of donor somatic cells with β‐mercaptoethanol (ME) or hemoglobin (Hb) improved in vitro‐development of bovine cloned embryos. This study was subsequently evaluated whether the exposure to Hb and/or ME during in vitro‐maturation or embryo culture could further promote the development of embryos cloned with ME‐treated donor cells. A prospective, randomized study was conducted and, embryo development, cell number, and apoptosis in blastocysts were monitored. A significant (P < 0.05) effect was found after the combined treatment of cloned embryos with Hb (1 μg/ml) and ME (10 μM); the development of morulae (53 vs. 35%) was greatly improved, which resulted in enhanced blastocyst formation (38%). However, cell number and apoptosis in blastocysts were predominantly affected by ME rather than Hb; a significant increase in total cell number of blastomeres (142–154 vs. 123 cells/embryo), inner cell mass (ICM) (39–41 vs. 27), and trophectoderm (TE) (103–114 vs. 98), and the ratio of ICM to TE cell number (0.26–0.27 vs. 0.22) was found. Also, the apoptosis index indicating the ratio of apoptotic cell to normal blastomere number was greatly reduced after ME treatments (0.85 vs. 0.056–0.069). When embryos cloned with ME‐treated cells were cultured in Hb + ME‐containing medium, any of the treatments to recipient oocytes before enucleation did not further promote the development. In conclusion, combined treatment of cloned embryos with Hb + ME not only improved in vitro‐development but also decreased blastomere apoptosis. The use of ME‐treated donor cells and the culture of cloned embryos in Hb + ME‐containing medium yielded the optimal results for promoting the production of blastocysts with improved quality. Mol. Reprod. Dev. 67:200–206, 2004. © 2004 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - cytology</subject><subject>Blastocyst - drug effects</subject><subject>Blastomeres - cytology</subject><subject>Blastomeres - drug effects</subject><subject>Cattle</subject><subject>Cloning, Organism</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Embryonic Development - drug effects</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hemoglobin</subject><subject>Hemoglobins - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Mercaptoethanol - pharmacology</subject><subject>Molecular embryology</subject><subject>Nuclear Transfer Techniques</subject><subject>preimplantation development</subject><subject>somatic cell nuclear transfer</subject><subject>β-mercaptoethanol</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU2O1DAQhSMEYn5gwQWQNyDNIoydxHG8RAPzIxqQYBCIjeWfCm1w4mC7e-iTcA8OwpnwTAKzYlWl0vdeSe8VxSOCnxGMq-MhmLzUvLpT7BPMu7JinN693htcNrT6tFccxPgVY8x5h-8Xe6RpedPU7X7x82KYgt-CQTCosPPIwBacnwYYE7qyaZ0POoCMmZCTn5KPNiI7IuVkTH6AABHJPkFAaQ0oZTTdaH2PtPNjlim_tSMs_nE2_f2rzFItsx-ktRy9Q3I0aA2D_-K8suOD4l4vXYSHyzwsPpy-vDw5L1dvzy5Onq9KXXe8KglVPda0alRFK95UmGLTM0VJA0bRrmV1y3uuFGtM1WHWGpBEA29ZL3FrCKkPi6ezb07h-wZiEoONGpyTI_hNFB3GDNe0yeDRDOrgYwzQiynYQYadIFhctyByC-Kmhcw-Xkw3agBzSy6xZ-DJAsiopeuDHLWNtxxltGM1zdzxzF1ZB7v_fxSv3734-7qcFTYm-PFPIcM3kcNgVHx8cybq1fklfv_5VLyq_wC5V7He</recordid><startdate>200402</startdate><enddate>200402</enddate><creator>Park, Eul Soon</creator><creator>Hwang, Woo Suk</creator><creator>Kang, Sung Keun</creator><creator>Lee, Byeong Chun</creator><creator>Han, Jae Yong</creator><creator>Lim, Jeong Mook</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200402</creationdate><title>Improved embryo development with decreased apoptosis in blastomeres after the treatment of cloned bovine embryos with β-mercaptoethanol and hemoglobin</title><author>Park, Eul Soon ; Hwang, Woo Suk ; Kang, Sung Keun ; Lee, Byeong Chun ; Han, Jae Yong ; Lim, Jeong Mook</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3892-15bf0c524b252942050df7b514edb5867369f9bb74d28076dea1ce967fa06d113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Biological and medical sciences</topic><topic>Blastocyst - cytology</topic><topic>Blastocyst - drug effects</topic><topic>Blastomeres - cytology</topic><topic>Blastomeres - drug effects</topic><topic>Cattle</topic><topic>Cloning, Organism</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Embryonic Development - drug effects</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hemoglobin</topic><topic>Hemoglobins - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Mercaptoethanol - pharmacology</topic><topic>Molecular embryology</topic><topic>Nuclear Transfer Techniques</topic><topic>preimplantation development</topic><topic>somatic cell nuclear transfer</topic><topic>β-mercaptoethanol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Eul Soon</creatorcontrib><creatorcontrib>Hwang, Woo Suk</creatorcontrib><creatorcontrib>Kang, Sung Keun</creatorcontrib><creatorcontrib>Lee, Byeong Chun</creatorcontrib><creatorcontrib>Han, Jae Yong</creatorcontrib><creatorcontrib>Lim, Jeong Mook</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Eul Soon</au><au>Hwang, Woo Suk</au><au>Kang, Sung Keun</au><au>Lee, Byeong Chun</au><au>Han, Jae Yong</au><au>Lim, Jeong Mook</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved embryo development with decreased apoptosis in blastomeres after the treatment of cloned bovine embryos with β-mercaptoethanol and hemoglobin</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>2004-02</date><risdate>2004</risdate><volume>67</volume><issue>2</issue><spage>200</spage><epage>206</epage><pages>200-206</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>In preliminary experiments, the treatment of donor somatic cells with β‐mercaptoethanol (ME) or hemoglobin (Hb) improved in vitro‐development of bovine cloned embryos. This study was subsequently evaluated whether the exposure to Hb and/or ME during in vitro‐maturation or embryo culture could further promote the development of embryos cloned with ME‐treated donor cells. A prospective, randomized study was conducted and, embryo development, cell number, and apoptosis in blastocysts were monitored. A significant (P < 0.05) effect was found after the combined treatment of cloned embryos with Hb (1 μg/ml) and ME (10 μM); the development of morulae (53 vs. 35%) was greatly improved, which resulted in enhanced blastocyst formation (38%). However, cell number and apoptosis in blastocysts were predominantly affected by ME rather than Hb; a significant increase in total cell number of blastomeres (142–154 vs. 123 cells/embryo), inner cell mass (ICM) (39–41 vs. 27), and trophectoderm (TE) (103–114 vs. 98), and the ratio of ICM to TE cell number (0.26–0.27 vs. 0.22) was found. Also, the apoptosis index indicating the ratio of apoptotic cell to normal blastomere number was greatly reduced after ME treatments (0.85 vs. 0.056–0.069). When embryos cloned with ME‐treated cells were cultured in Hb + ME‐containing medium, any of the treatments to recipient oocytes before enucleation did not further promote the development. In conclusion, combined treatment of cloned embryos with Hb + ME not only improved in vitro‐development but also decreased blastomere apoptosis. The use of ME‐treated donor cells and the culture of cloned embryos in Hb + ME‐containing medium yielded the optimal results for promoting the production of blastocysts with improved quality. Mol. Reprod. Dev. 67:200–206, 2004. © 2004 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>14694436</pmid><doi>10.1002/mrd.10392</doi><tpages>7</tpages></addata></record> |
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| ispartof | Molecular reproduction and development, 2004-02, Vol.67 (2), p.200-206 |
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| subjects | Animals apoptosis Apoptosis - drug effects Biological and medical sciences Blastocyst - cytology Blastocyst - drug effects Blastomeres - cytology Blastomeres - drug effects Cattle Cloning, Organism Embryology: invertebrates and vertebrates. Teratology Embryonic Development - drug effects Female Fundamental and applied biological sciences. Psychology hemoglobin Hemoglobins - pharmacology In Vitro Techniques Mercaptoethanol - pharmacology Molecular embryology Nuclear Transfer Techniques preimplantation development somatic cell nuclear transfer β-mercaptoethanol |
| title | Improved embryo development with decreased apoptosis in blastomeres after the treatment of cloned bovine embryos with β-mercaptoethanol and hemoglobin |
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