A fluorescence-based assay for multisubunit DNA-dependent RNA polymerases

The properties of DNA-dependent RNA polymerases have been studied since the 1960s, but considerable interest in probing RNA polymerase structure/function relationships, the roles of different classes of RNA polymerases in cellular processes, and the feasibility of using RNA polymerases as drug targe...

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Veröffentlicht in:	Analytical biochemistry 2004-01, Vol.324 (2), p.183-190
Hauptverfasser:	Kuhlman, Peter, Duff, Heidi L, Galant, Ashley
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA-Directed RNA Polymerases - analysis DNA-Directed RNA Polymerases - metabolism Fluorescence Fluorescent Dyes Kinetics Methods Microplate assay Non-radioactive detection RiboGreen RNA polymerase Solvents - pharmacology Transcription, Genetic
Online-Zugang:	Volltext
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