The effect of melanin bleaching on immunohistochemical staining in heavily pigmented melanocytic neoplasms
The accumulation of excessive amounts of melanin in melanocytic lesions can obscure cellular morphology and can further hinder immunocytochemical procedures. We have used a modification of the potassium permanganate/oxalic acid melanin-bleaching technique, involving much reduced bleaching times, in...
Gespeichert in:
| Veröffentlicht in: | The American journal of dermatopathology 1998-08, Vol.20 (4), p.357-361 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 361 |
|---|---|
| container_issue | 4 |
| container_start_page | 357 |
| container_title | The American journal of dermatopathology |
| container_volume | 20 |
| creator | ORCHARD, G. E CALONJE, E |
| description | The accumulation of excessive amounts of melanin in melanocytic lesions can obscure cellular morphology and can further hinder immunocytochemical procedures. We have used a modification of the potassium permanganate/oxalic acid melanin-bleaching technique, involving much reduced bleaching times, in order to remove melanin granules prior to incubation with primary antibody. We have assessed a panel of antibodies applicable to the evaluation of melanocytic lesions and in addition have also assessed antibodies that may be more useful in research. The study attempts to determine which antigens may be affected by bleaching and which are not. Antigens S100, HMB 45, NKIC3, CD34, and L26 are relatively unaffected by this procedure. Factor-VIII-related antigen and vimentin and CD68 antigens produced enhanced staining. In contrast, antigens CD3, CD31, and CD45RO were abolished. In addition, smooth muscle actin and desmin antigens demonstrated considerable nonspecific background staining and were not reliable in this study. This technique demonstrates that a fairly wide range of antigens are preserved after bleaching and that distinction between melanocytes and melanophages can reliably be performed using the conventional immunocytochemical chromogen 3,3-diaminobenzidine and without the need for elaborate counterstaining. |
| doi_str_mv | 10.1097/00000372-199808000-00006 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80069204</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>80069204</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-dc706be60cc890dc05123c378d77515dce7ece1e5408375b0de1d10f669021e23</originalsourceid><addsrcrecordid>eNo9kE9PwzAMxSMEGmPwEZByQNwKTrM27REh_kmTuIxzlbnumqlJRtMh7duTsrFcrPi9Z1s_xriABwGleoTxSZUmoiwLKOInGTv5GZuKTMoEpBDnbAqilEkMiEt2FcIGQKQFZBM2KdUYl1O2WbbEqWkIB-4bbqnTzji-6khja9yae8eNtTvnWxMGjy1Zg7rjYdDGjXo0t6R_TLfnW7O25AaqD2M87geD3JHfdjrYcM0uGt0FujnWGft6fVk-vyeLz7eP56dFgnPIhqRGBfmKckAsSqgRMpFKlKqolcpEViMpQhKUzaGQKltBTaIW0OR5CamgVM7Y_WHutvffOwpDZU1A6uJJ5HehirTyMoV5NBYHI_Y-hJ6aatsbq_t9JaAaMVf_mKsT5r9WHqO3xx27laX6FDxyjfrdUdch4mp67dCEky2VMleQyl9vyIY6</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80069204</pqid></control><display><type>article</type><title>The effect of melanin bleaching on immunohistochemical staining in heavily pigmented melanocytic neoplasms</title><source>MEDLINE</source><source>Journals@Ovid Complete</source><creator>ORCHARD, G. E ; CALONJE, E</creator><creatorcontrib>ORCHARD, G. E ; CALONJE, E</creatorcontrib><description>The accumulation of excessive amounts of melanin in melanocytic lesions can obscure cellular morphology and can further hinder immunocytochemical procedures. We have used a modification of the potassium permanganate/oxalic acid melanin-bleaching technique, involving much reduced bleaching times, in order to remove melanin granules prior to incubation with primary antibody. We have assessed a panel of antibodies applicable to the evaluation of melanocytic lesions and in addition have also assessed antibodies that may be more useful in research. The study attempts to determine which antigens may be affected by bleaching and which are not. Antigens S100, HMB 45, NKIC3, CD34, and L26 are relatively unaffected by this procedure. Factor-VIII-related antigen and vimentin and CD68 antigens produced enhanced staining. In contrast, antigens CD3, CD31, and CD45RO were abolished. In addition, smooth muscle actin and desmin antigens demonstrated considerable nonspecific background staining and were not reliable in this study. This technique demonstrates that a fairly wide range of antigens are preserved after bleaching and that distinction between melanocytes and melanophages can reliably be performed using the conventional immunocytochemical chromogen 3,3-diaminobenzidine and without the need for elaborate counterstaining.</description><identifier>ISSN: 0193-1091</identifier><identifier>EISSN: 1533-0311</identifier><identifier>DOI: 10.1097/00000372-199808000-00006</identifier><identifier>PMID: 9700373</identifier><identifier>CODEN: AJODDB</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>Actins - drug effects ; Actins - metabolism ; Antigens, CD - drug effects ; Antigens, CD - metabolism ; Antigens, CD20 - drug effects ; Antigens, CD20 - metabolism ; Antigens, CD34 - drug effects ; Antigens, CD34 - metabolism ; Antigens, Differentiation, Myelomonocytic - drug effects ; Antigens, Differentiation, Myelomonocytic - metabolism ; Antigens, Neoplasm - drug effects ; Antigens, Neoplasm - immunology ; Antigens, Neoplasm - metabolism ; Biological and medical sciences ; CD3 Complex - drug effects ; CD3 Complex - metabolism ; Desmin - drug effects ; Desmin - metabolism ; Humans ; Immunohistochemistry - methods ; Investigative techniques, diagnostic techniques (general aspects) ; Leukocyte Common Antigens - drug effects ; Leukocyte Common Antigens - metabolism ; Medical sciences ; Melanins - metabolism ; Melanoma - chemistry ; Melanoma - immunology ; Melanoma-Specific Antigens ; Miscellaneous. Technology ; Neoplasm Proteins - drug effects ; Neoplasm Proteins - metabolism ; Oxalates - pharmacology ; Oxidation-Reduction ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Pigmentation - drug effects ; Platelet Endothelial Cell Adhesion Molecule-1 - drug effects ; Platelet Endothelial Cell Adhesion Molecule-1 - metabolism ; Potassium Permanganate - pharmacology ; Reproducibility of Results ; S100 Proteins - drug effects ; S100 Proteins - metabolism ; Sensitivity and Specificity ; Skin - chemistry ; Skin - drug effects ; Skin - immunology ; Skin Neoplasms - chemistry ; Skin Neoplasms - immunology ; Vimentin - drug effects ; Vimentin - metabolism ; von Willebrand Factor - drug effects ; von Willebrand Factor - metabolism</subject><ispartof>The American journal of dermatopathology, 1998-08, Vol.20 (4), p.357-361</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-dc706be60cc890dc05123c378d77515dce7ece1e5408375b0de1d10f669021e23</citedby><cites>FETCH-LOGICAL-c405t-dc706be60cc890dc05123c378d77515dce7ece1e5408375b0de1d10f669021e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2336702$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9700373$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ORCHARD, G. E</creatorcontrib><creatorcontrib>CALONJE, E</creatorcontrib><title>The effect of melanin bleaching on immunohistochemical staining in heavily pigmented melanocytic neoplasms</title><title>The American journal of dermatopathology</title><addtitle>Am J Dermatopathol</addtitle><description>The accumulation of excessive amounts of melanin in melanocytic lesions can obscure cellular morphology and can further hinder immunocytochemical procedures. We have used a modification of the potassium permanganate/oxalic acid melanin-bleaching technique, involving much reduced bleaching times, in order to remove melanin granules prior to incubation with primary antibody. We have assessed a panel of antibodies applicable to the evaluation of melanocytic lesions and in addition have also assessed antibodies that may be more useful in research. The study attempts to determine which antigens may be affected by bleaching and which are not. Antigens S100, HMB 45, NKIC3, CD34, and L26 are relatively unaffected by this procedure. Factor-VIII-related antigen and vimentin and CD68 antigens produced enhanced staining. In contrast, antigens CD3, CD31, and CD45RO were abolished. In addition, smooth muscle actin and desmin antigens demonstrated considerable nonspecific background staining and were not reliable in this study. This technique demonstrates that a fairly wide range of antigens are preserved after bleaching and that distinction between melanocytes and melanophages can reliably be performed using the conventional immunocytochemical chromogen 3,3-diaminobenzidine and without the need for elaborate counterstaining.</description><subject>Actins - drug effects</subject><subject>Actins - metabolism</subject><subject>Antigens, CD - drug effects</subject><subject>Antigens, CD - metabolism</subject><subject>Antigens, CD20 - drug effects</subject><subject>Antigens, CD20 - metabolism</subject><subject>Antigens, CD34 - drug effects</subject><subject>Antigens, CD34 - metabolism</subject><subject>Antigens, Differentiation, Myelomonocytic - drug effects</subject><subject>Antigens, Differentiation, Myelomonocytic - metabolism</subject><subject>Antigens, Neoplasm - drug effects</subject><subject>Antigens, Neoplasm - immunology</subject><subject>Antigens, Neoplasm - metabolism</subject><subject>Biological and medical sciences</subject><subject>CD3 Complex - drug effects</subject><subject>CD3 Complex - metabolism</subject><subject>Desmin - drug effects</subject><subject>Desmin - metabolism</subject><subject>Humans</subject><subject>Immunohistochemistry - methods</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Leukocyte Common Antigens - drug effects</subject><subject>Leukocyte Common Antigens - metabolism</subject><subject>Medical sciences</subject><subject>Melanins - metabolism</subject><subject>Melanoma - chemistry</subject><subject>Melanoma - immunology</subject><subject>Melanoma-Specific Antigens</subject><subject>Miscellaneous. Technology</subject><subject>Neoplasm Proteins - drug effects</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Oxalates - pharmacology</subject><subject>Oxidation-Reduction</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Pigmentation - drug effects</subject><subject>Platelet Endothelial Cell Adhesion Molecule-1 - drug effects</subject><subject>Platelet Endothelial Cell Adhesion Molecule-1 - metabolism</subject><subject>Potassium Permanganate - pharmacology</subject><subject>Reproducibility of Results</subject><subject>S100 Proteins - drug effects</subject><subject>S100 Proteins - metabolism</subject><subject>Sensitivity and Specificity</subject><subject>Skin - chemistry</subject><subject>Skin - drug effects</subject><subject>Skin - immunology</subject><subject>Skin Neoplasms - chemistry</subject><subject>Skin Neoplasms - immunology</subject><subject>Vimentin - drug effects</subject><subject>Vimentin - metabolism</subject><subject>von Willebrand Factor - drug effects</subject><subject>von Willebrand Factor - metabolism</subject><issn>0193-1091</issn><issn>1533-0311</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE9PwzAMxSMEGmPwEZByQNwKTrM27REh_kmTuIxzlbnumqlJRtMh7duTsrFcrPi9Z1s_xriABwGleoTxSZUmoiwLKOInGTv5GZuKTMoEpBDnbAqilEkMiEt2FcIGQKQFZBM2KdUYl1O2WbbEqWkIB-4bbqnTzji-6khja9yae8eNtTvnWxMGjy1Zg7rjYdDGjXo0t6R_TLfnW7O25AaqD2M87geD3JHfdjrYcM0uGt0FujnWGft6fVk-vyeLz7eP56dFgnPIhqRGBfmKckAsSqgRMpFKlKqolcpEViMpQhKUzaGQKltBTaIW0OR5CamgVM7Y_WHutvffOwpDZU1A6uJJ5HehirTyMoV5NBYHI_Y-hJ6aatsbq_t9JaAaMVf_mKsT5r9WHqO3xx27laX6FDxyjfrdUdch4mp67dCEky2VMleQyl9vyIY6</recordid><startdate>19980801</startdate><enddate>19980801</enddate><creator>ORCHARD, G. E</creator><creator>CALONJE, E</creator><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980801</creationdate><title>The effect of melanin bleaching on immunohistochemical staining in heavily pigmented melanocytic neoplasms</title><author>ORCHARD, G. E ; CALONJE, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-dc706be60cc890dc05123c378d77515dce7ece1e5408375b0de1d10f669021e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Actins - drug effects</topic><topic>Actins - metabolism</topic><topic>Antigens, CD - drug effects</topic><topic>Antigens, CD - metabolism</topic><topic>Antigens, CD20 - drug effects</topic><topic>Antigens, CD20 - metabolism</topic><topic>Antigens, CD34 - drug effects</topic><topic>Antigens, CD34 - metabolism</topic><topic>Antigens, Differentiation, Myelomonocytic - drug effects</topic><topic>Antigens, Differentiation, Myelomonocytic - metabolism</topic><topic>Antigens, Neoplasm - drug effects</topic><topic>Antigens, Neoplasm - immunology</topic><topic>Antigens, Neoplasm - metabolism</topic><topic>Biological and medical sciences</topic><topic>CD3 Complex - drug effects</topic><topic>CD3 Complex - metabolism</topic><topic>Desmin - drug effects</topic><topic>Desmin - metabolism</topic><topic>Humans</topic><topic>Immunohistochemistry - methods</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Leukocyte Common Antigens - drug effects</topic><topic>Leukocyte Common Antigens - metabolism</topic><topic>Medical sciences</topic><topic>Melanins - metabolism</topic><topic>Melanoma - chemistry</topic><topic>Melanoma - immunology</topic><topic>Melanoma-Specific Antigens</topic><topic>Miscellaneous. Technology</topic><topic>Neoplasm Proteins - drug effects</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Oxalates - pharmacology</topic><topic>Oxidation-Reduction</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Pigmentation - drug effects</topic><topic>Platelet Endothelial Cell Adhesion Molecule-1 - drug effects</topic><topic>Platelet Endothelial Cell Adhesion Molecule-1 - metabolism</topic><topic>Potassium Permanganate - pharmacology</topic><topic>Reproducibility of Results</topic><topic>S100 Proteins - drug effects</topic><topic>S100 Proteins - metabolism</topic><topic>Sensitivity and Specificity</topic><topic>Skin - chemistry</topic><topic>Skin - drug effects</topic><topic>Skin - immunology</topic><topic>Skin Neoplasms - chemistry</topic><topic>Skin Neoplasms - immunology</topic><topic>Vimentin - drug effects</topic><topic>Vimentin - metabolism</topic><topic>von Willebrand Factor - drug effects</topic><topic>von Willebrand Factor - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ORCHARD, G. E</creatorcontrib><creatorcontrib>CALONJE, E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of dermatopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ORCHARD, G. E</au><au>CALONJE, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of melanin bleaching on immunohistochemical staining in heavily pigmented melanocytic neoplasms</atitle><jtitle>The American journal of dermatopathology</jtitle><addtitle>Am J Dermatopathol</addtitle><date>1998-08-01</date><risdate>1998</risdate><volume>20</volume><issue>4</issue><spage>357</spage><epage>361</epage><pages>357-361</pages><issn>0193-1091</issn><eissn>1533-0311</eissn><coden>AJODDB</coden><abstract>The accumulation of excessive amounts of melanin in melanocytic lesions can obscure cellular morphology and can further hinder immunocytochemical procedures. We have used a modification of the potassium permanganate/oxalic acid melanin-bleaching technique, involving much reduced bleaching times, in order to remove melanin granules prior to incubation with primary antibody. We have assessed a panel of antibodies applicable to the evaluation of melanocytic lesions and in addition have also assessed antibodies that may be more useful in research. The study attempts to determine which antigens may be affected by bleaching and which are not. Antigens S100, HMB 45, NKIC3, CD34, and L26 are relatively unaffected by this procedure. Factor-VIII-related antigen and vimentin and CD68 antigens produced enhanced staining. In contrast, antigens CD3, CD31, and CD45RO were abolished. In addition, smooth muscle actin and desmin antigens demonstrated considerable nonspecific background staining and were not reliable in this study. This technique demonstrates that a fairly wide range of antigens are preserved after bleaching and that distinction between melanocytes and melanophages can reliably be performed using the conventional immunocytochemical chromogen 3,3-diaminobenzidine and without the need for elaborate counterstaining.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>9700373</pmid><doi>10.1097/00000372-199808000-00006</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0193-1091 |
| ispartof | The American journal of dermatopathology, 1998-08, Vol.20 (4), p.357-361 |
| issn | 0193-1091 1533-0311 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80069204 |
| source | MEDLINE; Journals@Ovid Complete |
| subjects | Actins - drug effects Actins - metabolism Antigens, CD - drug effects Antigens, CD - metabolism Antigens, CD20 - drug effects Antigens, CD20 - metabolism Antigens, CD34 - drug effects Antigens, CD34 - metabolism Antigens, Differentiation, Myelomonocytic - drug effects Antigens, Differentiation, Myelomonocytic - metabolism Antigens, Neoplasm - drug effects Antigens, Neoplasm - immunology Antigens, Neoplasm - metabolism Biological and medical sciences CD3 Complex - drug effects CD3 Complex - metabolism Desmin - drug effects Desmin - metabolism Humans Immunohistochemistry - methods Investigative techniques, diagnostic techniques (general aspects) Leukocyte Common Antigens - drug effects Leukocyte Common Antigens - metabolism Medical sciences Melanins - metabolism Melanoma - chemistry Melanoma - immunology Melanoma-Specific Antigens Miscellaneous. Technology Neoplasm Proteins - drug effects Neoplasm Proteins - metabolism Oxalates - pharmacology Oxidation-Reduction Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Pigmentation - drug effects Platelet Endothelial Cell Adhesion Molecule-1 - drug effects Platelet Endothelial Cell Adhesion Molecule-1 - metabolism Potassium Permanganate - pharmacology Reproducibility of Results S100 Proteins - drug effects S100 Proteins - metabolism Sensitivity and Specificity Skin - chemistry Skin - drug effects Skin - immunology Skin Neoplasms - chemistry Skin Neoplasms - immunology Vimentin - drug effects Vimentin - metabolism von Willebrand Factor - drug effects von Willebrand Factor - metabolism |
| title | The effect of melanin bleaching on immunohistochemical staining in heavily pigmented melanocytic neoplasms |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T21%3A04%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20effect%20of%20melanin%20bleaching%20on%20immunohistochemical%20staining%20in%20heavily%20pigmented%20melanocytic%20neoplasms&rft.jtitle=The%20American%20journal%20of%20dermatopathology&rft.au=ORCHARD,%20G.%20E&rft.date=1998-08-01&rft.volume=20&rft.issue=4&rft.spage=357&rft.epage=361&rft.pages=357-361&rft.issn=0193-1091&rft.eissn=1533-0311&rft.coden=AJODDB&rft_id=info:doi/10.1097/00000372-199808000-00006&rft_dat=%3Cproquest_cross%3E80069204%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=80069204&rft_id=info:pmid/9700373&rfr_iscdi=true |