Enzyme-linked immunosorbent assay for circulating human resistin: resistin concentrations in normal subjects and patients with type 2 diabetes
Background: Resistin is a recently identified adipocyte-secreted hormone in rodents, and has been proposed to serve as a link between obesity and insulin resistance. The aim of this study was to develop a sensitive enzyme-linked immunosorbent assay (ELISA) for human resistin and evaluate serum resis...
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| Veröffentlicht in: | Clinica chimica acta 2004, Vol.339 (1), p.57-63 |
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| creator | Fujinami, Aya Obayashi, Hiroshi Ohta, Kiyoe Ichimura, Toshiko Nishimura, Masataka Matsui, Hidehito Kawahara, Yuji Yamazaki, Masahiro Ogata, Masakazu Hasegawa, Goji Nakamura, Naoto Yoshikawa, Toshikazu Nakano, Koji Ohta, Mitsuhiro |
| description | Background: Resistin is a recently identified adipocyte-secreted hormone in rodents, and has been proposed to serve as a link between obesity and insulin resistance. The aim of this study was to develop a sensitive enzyme-linked immunosorbent assay (ELISA) for human resistin and evaluate serum resistin concentrations in normal subjects and patients with type 2 diabetes.
Methods: Using ELISA developed by two polyclonal antibodies, resistin concentrations were measured in 90 patients with type 2 diabetes and compared to 74 healthy control subjects.
Results: This ELISA has high specificity and sensitivity over the concentration of range 0.5–100 ng/ml with good percentage recovery (97.1±4.7%) and reproducibility (within-day assay, CV=4.8–8.6%; between-day assay, CV=5.6–9.7%). The mean concentration of resistin in sera from type 2 diabetic patients was significantly higher than that in normal subjects (mean±S.E.: 20.8±0.7 vs. 14.9±0.5 ng/ml,
p |
| doi_str_mv | 10.1016/j.cccn.2003.09.009 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80066311</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0009898103004522</els_id><sourcerecordid>80066311</sourcerecordid><originalsourceid>FETCH-LOGICAL-c354t-6c9aa186f92345a1b6cf7f3844bd8304d15445258dce9cda4ae11dd25843e5d43</originalsourceid><addsrcrecordid>eNp9kcFu3CAURVHUKJmm-YEuKlbd2QWDPRB1U0VJWilSNu0aYXhumNow4dmNJh_Rbw6jGbW7ruBdzn0S9xLynrOaM9592tTOuVg3jIma6ZoxfUJWXK1FJaRu3pAVK1KltOLn5C3ipoySdfyMnHPZqbXSckX-3MSX3QTVGOIv8DRM0xITptxDnKlFtDs6pExdyG4Z7RziT_q4TDbSDBiwzFd_b9Sl6IotFyxFpEWJKU92pLj0G3AzUhs93ZbnQiF9DvMjnXdboA31wfYwA74jp4MdES6P5wX5cXvz_fprdf9w9-36y33lRCvnqnPaWq66QTdCtpb3nRvWg1BS9l4JJj1vpWybVnkH2nkrLXDufRGkgNZLcUE-HvZuc3paAGczBXQwjjZCWtAoxrpOcF7A5gC6nBAzDGabw2TzznBm9i2Yjdm3YPYtGKZNibyYPhy3L_0E_p_lGHsBPh8AKH_8HSAbdCUUBz7kEpTxKfxv_yvxqJym</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80066311</pqid></control><display><type>article</type><title>Enzyme-linked immunosorbent assay for circulating human resistin: resistin concentrations in normal subjects and patients with type 2 diabetes</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Fujinami, Aya ; Obayashi, Hiroshi ; Ohta, Kiyoe ; Ichimura, Toshiko ; Nishimura, Masataka ; Matsui, Hidehito ; Kawahara, Yuji ; Yamazaki, Masahiro ; Ogata, Masakazu ; Hasegawa, Goji ; Nakamura, Naoto ; Yoshikawa, Toshikazu ; Nakano, Koji ; Ohta, Mitsuhiro</creator><creatorcontrib>Fujinami, Aya ; Obayashi, Hiroshi ; Ohta, Kiyoe ; Ichimura, Toshiko ; Nishimura, Masataka ; Matsui, Hidehito ; Kawahara, Yuji ; Yamazaki, Masahiro ; Ogata, Masakazu ; Hasegawa, Goji ; Nakamura, Naoto ; Yoshikawa, Toshikazu ; Nakano, Koji ; Ohta, Mitsuhiro</creatorcontrib><description>Background: Resistin is a recently identified adipocyte-secreted hormone in rodents, and has been proposed to serve as a link between obesity and insulin resistance. The aim of this study was to develop a sensitive enzyme-linked immunosorbent assay (ELISA) for human resistin and evaluate serum resistin concentrations in normal subjects and patients with type 2 diabetes.
Methods: Using ELISA developed by two polyclonal antibodies, resistin concentrations were measured in 90 patients with type 2 diabetes and compared to 74 healthy control subjects.
Results: This ELISA has high specificity and sensitivity over the concentration of range 0.5–100 ng/ml with good percentage recovery (97.1±4.7%) and reproducibility (within-day assay, CV=4.8–8.6%; between-day assay, CV=5.6–9.7%). The mean concentration of resistin in sera from type 2 diabetic patients was significantly higher than that in normal subjects (mean±S.E.: 20.8±0.7 vs. 14.9±0.5 ng/ml,
p<0.001). A moderate positive correlation was observed between serum resistin levels and body mass indices in both normal subjects (
r=0.412,
p<0.0003) and patients with type 2 diabetes (
r=0.395,
p<0.0001).
Conclusions: Our ELISA will be useful to confirm the physiological and pathophysiological role of resistin in humans.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/j.cccn.2003.09.009</identifier><identifier>PMID: 14687894</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adipose Tissue - metabolism ; Anti-human resistin antiserum ; Antibodies - immunology ; Body mass index (BMI) ; Body Weight ; Diabetes Mellitus, Type 2 - blood ; Enzyme-linked immunosorbent assay (ELISA) ; Enzyme-Linked Immunosorbent Assay - methods ; Hormones, Ectopic - blood ; Hormones, Ectopic - immunology ; Humans ; Obesity ; Resistin ; Type 2 diabetes mellitus</subject><ispartof>Clinica chimica acta, 2004, Vol.339 (1), p.57-63</ispartof><rights>2003 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-6c9aa186f92345a1b6cf7f3844bd8304d15445258dce9cda4ae11dd25843e5d43</citedby><cites>FETCH-LOGICAL-c354t-6c9aa186f92345a1b6cf7f3844bd8304d15445258dce9cda4ae11dd25843e5d43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cccn.2003.09.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14687894$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujinami, Aya</creatorcontrib><creatorcontrib>Obayashi, Hiroshi</creatorcontrib><creatorcontrib>Ohta, Kiyoe</creatorcontrib><creatorcontrib>Ichimura, Toshiko</creatorcontrib><creatorcontrib>Nishimura, Masataka</creatorcontrib><creatorcontrib>Matsui, Hidehito</creatorcontrib><creatorcontrib>Kawahara, Yuji</creatorcontrib><creatorcontrib>Yamazaki, Masahiro</creatorcontrib><creatorcontrib>Ogata, Masakazu</creatorcontrib><creatorcontrib>Hasegawa, Goji</creatorcontrib><creatorcontrib>Nakamura, Naoto</creatorcontrib><creatorcontrib>Yoshikawa, Toshikazu</creatorcontrib><creatorcontrib>Nakano, Koji</creatorcontrib><creatorcontrib>Ohta, Mitsuhiro</creatorcontrib><title>Enzyme-linked immunosorbent assay for circulating human resistin: resistin concentrations in normal subjects and patients with type 2 diabetes</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Background: Resistin is a recently identified adipocyte-secreted hormone in rodents, and has been proposed to serve as a link between obesity and insulin resistance. The aim of this study was to develop a sensitive enzyme-linked immunosorbent assay (ELISA) for human resistin and evaluate serum resistin concentrations in normal subjects and patients with type 2 diabetes.
Methods: Using ELISA developed by two polyclonal antibodies, resistin concentrations were measured in 90 patients with type 2 diabetes and compared to 74 healthy control subjects.
Results: This ELISA has high specificity and sensitivity over the concentration of range 0.5–100 ng/ml with good percentage recovery (97.1±4.7%) and reproducibility (within-day assay, CV=4.8–8.6%; between-day assay, CV=5.6–9.7%). The mean concentration of resistin in sera from type 2 diabetic patients was significantly higher than that in normal subjects (mean±S.E.: 20.8±0.7 vs. 14.9±0.5 ng/ml,
p<0.001). A moderate positive correlation was observed between serum resistin levels and body mass indices in both normal subjects (
r=0.412,
p<0.0003) and patients with type 2 diabetes (
r=0.395,
p<0.0001).
Conclusions: Our ELISA will be useful to confirm the physiological and pathophysiological role of resistin in humans.</description><subject>Adipose Tissue - metabolism</subject><subject>Anti-human resistin antiserum</subject><subject>Antibodies - immunology</subject><subject>Body mass index (BMI)</subject><subject>Body Weight</subject><subject>Diabetes Mellitus, Type 2 - blood</subject><subject>Enzyme-linked immunosorbent assay (ELISA)</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Hormones, Ectopic - blood</subject><subject>Hormones, Ectopic - immunology</subject><subject>Humans</subject><subject>Obesity</subject><subject>Resistin</subject><subject>Type 2 diabetes mellitus</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu3CAURVHUKJmm-YEuKlbd2QWDPRB1U0VJWilSNu0aYXhumNow4dmNJh_Rbw6jGbW7ruBdzn0S9xLynrOaM9592tTOuVg3jIma6ZoxfUJWXK1FJaRu3pAVK1KltOLn5C3ipoySdfyMnHPZqbXSckX-3MSX3QTVGOIv8DRM0xITptxDnKlFtDs6pExdyG4Z7RziT_q4TDbSDBiwzFd_b9Sl6IotFyxFpEWJKU92pLj0G3AzUhs93ZbnQiF9DvMjnXdboA31wfYwA74jp4MdES6P5wX5cXvz_fprdf9w9-36y33lRCvnqnPaWq66QTdCtpb3nRvWg1BS9l4JJj1vpWybVnkH2nkrLXDufRGkgNZLcUE-HvZuc3paAGczBXQwjjZCWtAoxrpOcF7A5gC6nBAzDGabw2TzznBm9i2Yjdm3YPYtGKZNibyYPhy3L_0E_p_lGHsBPh8AKH_8HSAbdCUUBz7kEpTxKfxv_yvxqJym</recordid><startdate>2004</startdate><enddate>2004</enddate><creator>Fujinami, Aya</creator><creator>Obayashi, Hiroshi</creator><creator>Ohta, Kiyoe</creator><creator>Ichimura, Toshiko</creator><creator>Nishimura, Masataka</creator><creator>Matsui, Hidehito</creator><creator>Kawahara, Yuji</creator><creator>Yamazaki, Masahiro</creator><creator>Ogata, Masakazu</creator><creator>Hasegawa, Goji</creator><creator>Nakamura, Naoto</creator><creator>Yoshikawa, Toshikazu</creator><creator>Nakano, Koji</creator><creator>Ohta, Mitsuhiro</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2004</creationdate><title>Enzyme-linked immunosorbent assay for circulating human resistin: resistin concentrations in normal subjects and patients with type 2 diabetes</title><author>Fujinami, Aya ; Obayashi, Hiroshi ; Ohta, Kiyoe ; Ichimura, Toshiko ; Nishimura, Masataka ; Matsui, Hidehito ; Kawahara, Yuji ; Yamazaki, Masahiro ; Ogata, Masakazu ; Hasegawa, Goji ; Nakamura, Naoto ; Yoshikawa, Toshikazu ; Nakano, Koji ; Ohta, Mitsuhiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-6c9aa186f92345a1b6cf7f3844bd8304d15445258dce9cda4ae11dd25843e5d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adipose Tissue - metabolism</topic><topic>Anti-human resistin antiserum</topic><topic>Antibodies - immunology</topic><topic>Body mass index (BMI)</topic><topic>Body Weight</topic><topic>Diabetes Mellitus, Type 2 - blood</topic><topic>Enzyme-linked immunosorbent assay (ELISA)</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Hormones, Ectopic - blood</topic><topic>Hormones, Ectopic - immunology</topic><topic>Humans</topic><topic>Obesity</topic><topic>Resistin</topic><topic>Type 2 diabetes mellitus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujinami, Aya</creatorcontrib><creatorcontrib>Obayashi, Hiroshi</creatorcontrib><creatorcontrib>Ohta, Kiyoe</creatorcontrib><creatorcontrib>Ichimura, Toshiko</creatorcontrib><creatorcontrib>Nishimura, Masataka</creatorcontrib><creatorcontrib>Matsui, Hidehito</creatorcontrib><creatorcontrib>Kawahara, Yuji</creatorcontrib><creatorcontrib>Yamazaki, Masahiro</creatorcontrib><creatorcontrib>Ogata, Masakazu</creatorcontrib><creatorcontrib>Hasegawa, Goji</creatorcontrib><creatorcontrib>Nakamura, Naoto</creatorcontrib><creatorcontrib>Yoshikawa, Toshikazu</creatorcontrib><creatorcontrib>Nakano, Koji</creatorcontrib><creatorcontrib>Ohta, Mitsuhiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujinami, Aya</au><au>Obayashi, Hiroshi</au><au>Ohta, Kiyoe</au><au>Ichimura, Toshiko</au><au>Nishimura, Masataka</au><au>Matsui, Hidehito</au><au>Kawahara, Yuji</au><au>Yamazaki, Masahiro</au><au>Ogata, Masakazu</au><au>Hasegawa, Goji</au><au>Nakamura, Naoto</au><au>Yoshikawa, Toshikazu</au><au>Nakano, Koji</au><au>Ohta, Mitsuhiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzyme-linked immunosorbent assay for circulating human resistin: resistin concentrations in normal subjects and patients with type 2 diabetes</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>2004</date><risdate>2004</risdate><volume>339</volume><issue>1</issue><spage>57</spage><epage>63</epage><pages>57-63</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>Background: Resistin is a recently identified adipocyte-secreted hormone in rodents, and has been proposed to serve as a link between obesity and insulin resistance. The aim of this study was to develop a sensitive enzyme-linked immunosorbent assay (ELISA) for human resistin and evaluate serum resistin concentrations in normal subjects and patients with type 2 diabetes.
Methods: Using ELISA developed by two polyclonal antibodies, resistin concentrations were measured in 90 patients with type 2 diabetes and compared to 74 healthy control subjects.
Results: This ELISA has high specificity and sensitivity over the concentration of range 0.5–100 ng/ml with good percentage recovery (97.1±4.7%) and reproducibility (within-day assay, CV=4.8–8.6%; between-day assay, CV=5.6–9.7%). The mean concentration of resistin in sera from type 2 diabetic patients was significantly higher than that in normal subjects (mean±S.E.: 20.8±0.7 vs. 14.9±0.5 ng/ml,
p<0.001). A moderate positive correlation was observed between serum resistin levels and body mass indices in both normal subjects (
r=0.412,
p<0.0003) and patients with type 2 diabetes (
r=0.395,
p<0.0001).
Conclusions: Our ELISA will be useful to confirm the physiological and pathophysiological role of resistin in humans.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>14687894</pmid><doi>10.1016/j.cccn.2003.09.009</doi><tpages>7</tpages></addata></record> |
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| ispartof | Clinica chimica acta, 2004, Vol.339 (1), p.57-63 |
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| subjects | Adipose Tissue - metabolism Anti-human resistin antiserum Antibodies - immunology Body mass index (BMI) Body Weight Diabetes Mellitus, Type 2 - blood Enzyme-linked immunosorbent assay (ELISA) Enzyme-Linked Immunosorbent Assay - methods Hormones, Ectopic - blood Hormones, Ectopic - immunology Humans Obesity Resistin Type 2 diabetes mellitus |
| title | Enzyme-linked immunosorbent assay for circulating human resistin: resistin concentrations in normal subjects and patients with type 2 diabetes |
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