Neuraminidase treatment modifies the function of electroplax sodium channels in planar lipid bilayers

Sodium channels from several sources are covalently modified by unusually large numbers of negatively charged sialic acid residues. In the present studies, purified electroplax sodium channels were treated with neuraminidase to remove sialic acid residues and then examined for functional changes in...

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Veröffentlicht in:	Neuron (Cambridge, Mass.) Mass.), 1990-11, Vol.5 (5), p.675-684
Hauptverfasser:	Recio-Pinto, Esperanza, Thornhill, William B., Duch, Daniel S., Levinson, Simon R., Urban, Bernd W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Eels Electric Conductivity electric organs Homeostasis Ion Channel Gating Lipid Bilayers Neuraminidase - pharmacology sodium Sodium Channels - drug effects Sodium Channels - physiology Time Factors
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container_title	Neuron (Cambridge, Mass.)
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creator	Recio-Pinto, Esperanza Thornhill, William B. Duch, Daniel S. Levinson, Simon R. Urban, Bernd W.
description	Sodium channels from several sources are covalently modified by unusually large numbers of negatively charged sialic acid residues. In the present studies, purified electroplax sodium channels were treated with neuraminidase to remove sialic acid residues and then examined for functional changes in planar lipid bilayers. Neuraminidase treatment resulted in a large depolarizing shift in the average potential required for channel activation. Additionally, desialidated channels showed a striking increase in the frequency of reversible transitions to subconductance states. Thus it appears that sialic acid residues play a significant role in the function of sodium channels, possibly through their influence on the local electric field and/or conformational stability of the channel molecule.
doi_str_mv	10.1016/0896-6273(90)90221-Z
format	Article
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subjects	Animals Eels Electric Conductivity electric organs Homeostasis Ion Channel Gating Lipid Bilayers Neuraminidase - pharmacology sodium Sodium Channels - drug effects Sodium Channels - physiology Time Factors
title	Neuraminidase treatment modifies the function of electroplax sodium channels in planar lipid bilayers
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