Biochemical and immunochemical characterisation of strains of Treponema hyodysenteriae
The protein composition of 18 clinical isolates of Treponema hyodysenteriae from pigs with swine dysebtery in Australia were compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunublot analysis. Coomassie Blue stained SDS-PAGE-profiles of whole cell and outer memb...
Gespeichert in:
| Veröffentlicht in: | Veterinary microbiology 1990-07, Vol.24 (1), p.29-41 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 41 |
|---|---|
| container_issue | 1 |
| container_start_page | 29 |
| container_title | Veterinary microbiology |
| container_volume | 24 |
| creator | Smith, Stuart C. Roddick, Felicity Ling, Soong Gerraty, Norman L. Coloe, Peter J. |
| description | The protein composition of 18 clinical isolates of
Treponema hyodysenteriae from pigs with swine dysebtery in Australia were compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunublot analysis. Coomassie Blue stained SDS-PAGE-profiles of whole cell and outer membrane (OM) proteins demonstrated the same gel pattern among the
T. hyodysenteriae isolates, particularly the OM proteins in the molecular mass (M
r) range of 30 kDa to 40 kDa. The
T. hyodysenteriae isolates were categorised into two distinct groups (A and B) based on the strain-variability in the 37 kDa OM protein. Immunoblotting of whole cell proteins after SDS-PAGE using serum from rabbits and pigs immunised with known
T. hyodysenteriae serotypes revealed a number of common immunoreactive bands in all isolates. LPS typing of the
T. hyodysenteriae isolates by immunoblotting with the rabbit antiserum revealed one additional serotype emphasising the LPS heterogeneity among the strains isolated from geographic locations in Australia, Great Britain and the U.S.A. Immunoblotting of the OM preparations revealed several common immunoreactive polypeptides corresponding to M
r values of 34 kDa to 30 kDa among the
T. hyodysenteriae and
T. innocens isolates but a distinct 39 kDa found only in the
T. hyodysenteriae isolates. Trypsin proteolysis of intact
T. hyodysenteriae cells caused selective loss of these and other major abundant proteins identifying the location of the 39 kDa, 36 kDa,and 30 kDa proteins on the cell surface and suggesting a possible role of these proteins in the pathogenesis of swine dysentery. |
| doi_str_mv | 10.1016/0378-1135(90)90048-Z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80060831</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>037811359090048Z</els_id><sourcerecordid>15790789</sourcerecordid><originalsourceid>FETCH-LOGICAL-c437t-db6ba68252230584927b32ea62646d9cc17619a69f93a3b2a46524ef87d2a4433</originalsourceid><addsrcrecordid>eNqFkM1u1DAUha0KVKalL1CBlA0IFgH_xbE3laDiTxrBpnTRjXXj3HRcTeypnUGat6_DjNodrHzl890j-yPkFaMfGGXqIxWtrhkTzTtD3xtKpa5vjsiC6VbUvJH8GVk8Ii_ISc53tEBG0WNyzDkzSokFuf7so1vh6B2sKwh95cdxG56u3AoSuAmTzzD5GKo4VHlK4EOex6uEmxhwhGq1i_0uY5hRwJfk-QDrjGeH85T8_vrl6vJ7vfz17cflp2XtpGinuu9UB0rzhnNBGy0NbzvBERRXUvXGOdYqZkCZwQgQHQepGi5x0G1fZinEKXm7792keL_FPNnRZ4frNQSM22w1pYpqwf4LsqY1tNWmgHIPuhRzTjjYTfIjpJ1l1M7e7SzVzlKtofavd3tT1l4f-rfdiP3j0kF0yd8ccsjF65AgOJ-fuo0QrKG8cOd7boBo4bZotz-XhlGu9fyJi32IRekfj8lm5zE47H1CN9k--n-_8gG9zqbS</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15790789</pqid></control><display><type>article</type><title>Biochemical and immunochemical characterisation of strains of Treponema hyodysenteriae</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Smith, Stuart C. ; Roddick, Felicity ; Ling, Soong ; Gerraty, Norman L. ; Coloe, Peter J.</creator><creatorcontrib>Smith, Stuart C. ; Roddick, Felicity ; Ling, Soong ; Gerraty, Norman L. ; Coloe, Peter J.</creatorcontrib><description>The protein composition of 18 clinical isolates of
Treponema hyodysenteriae from pigs with swine dysebtery in Australia were compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunublot analysis. Coomassie Blue stained SDS-PAGE-profiles of whole cell and outer membrane (OM) proteins demonstrated the same gel pattern among the
T. hyodysenteriae isolates, particularly the OM proteins in the molecular mass (M
r) range of 30 kDa to 40 kDa. The
T. hyodysenteriae isolates were categorised into two distinct groups (A and B) based on the strain-variability in the 37 kDa OM protein. Immunoblotting of whole cell proteins after SDS-PAGE using serum from rabbits and pigs immunised with known
T. hyodysenteriae serotypes revealed a number of common immunoreactive bands in all isolates. LPS typing of the
T. hyodysenteriae isolates by immunoblotting with the rabbit antiserum revealed one additional serotype emphasising the LPS heterogeneity among the strains isolated from geographic locations in Australia, Great Britain and the U.S.A. Immunoblotting of the OM preparations revealed several common immunoreactive polypeptides corresponding to M
r values of 34 kDa to 30 kDa among the
T. hyodysenteriae and
T. innocens isolates but a distinct 39 kDa found only in the
T. hyodysenteriae isolates. Trypsin proteolysis of intact
T. hyodysenteriae cells caused selective loss of these and other major abundant proteins identifying the location of the 39 kDa, 36 kDa,and 30 kDa proteins on the cell surface and suggesting a possible role of these proteins in the pathogenesis of swine dysentery.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/0378-1135(90)90048-Z</identifier><identifier>PMID: 2219663</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Australia ; Bacterial Outer Membrane Proteins - analysis ; Bacterial Proteins - analysis ; Bacteriology ; Biological and medical sciences ; Blotting, Western ; CERDO ; DISENTERIA PORCINA ; DYSENTERIE DU PORC ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; IDENTIFICACION ; IDENTIFICATION ; Microbiology ; PORCIN ; SWINE ; SWINE DYSENTERY ; TREPONEMA ; Treponema - analysis ; United Kingdom ; United States ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><ispartof>Veterinary microbiology, 1990-07, Vol.24 (1), p.29-41</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-db6ba68252230584927b32ea62646d9cc17619a69f93a3b2a46524ef87d2a4433</citedby><cites>FETCH-LOGICAL-c437t-db6ba68252230584927b32ea62646d9cc17619a69f93a3b2a46524ef87d2a4433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/037811359090048Z$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19331502$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2219663$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smith, Stuart C.</creatorcontrib><creatorcontrib>Roddick, Felicity</creatorcontrib><creatorcontrib>Ling, Soong</creatorcontrib><creatorcontrib>Gerraty, Norman L.</creatorcontrib><creatorcontrib>Coloe, Peter J.</creatorcontrib><title>Biochemical and immunochemical characterisation of strains of Treponema hyodysenteriae</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>The protein composition of 18 clinical isolates of
Treponema hyodysenteriae from pigs with swine dysebtery in Australia were compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunublot analysis. Coomassie Blue stained SDS-PAGE-profiles of whole cell and outer membrane (OM) proteins demonstrated the same gel pattern among the
T. hyodysenteriae isolates, particularly the OM proteins in the molecular mass (M
r) range of 30 kDa to 40 kDa. The
T. hyodysenteriae isolates were categorised into two distinct groups (A and B) based on the strain-variability in the 37 kDa OM protein. Immunoblotting of whole cell proteins after SDS-PAGE using serum from rabbits and pigs immunised with known
T. hyodysenteriae serotypes revealed a number of common immunoreactive bands in all isolates. LPS typing of the
T. hyodysenteriae isolates by immunoblotting with the rabbit antiserum revealed one additional serotype emphasising the LPS heterogeneity among the strains isolated from geographic locations in Australia, Great Britain and the U.S.A. Immunoblotting of the OM preparations revealed several common immunoreactive polypeptides corresponding to M
r values of 34 kDa to 30 kDa among the
T. hyodysenteriae and
T. innocens isolates but a distinct 39 kDa found only in the
T. hyodysenteriae isolates. Trypsin proteolysis of intact
T. hyodysenteriae cells caused selective loss of these and other major abundant proteins identifying the location of the 39 kDa, 36 kDa,and 30 kDa proteins on the cell surface and suggesting a possible role of these proteins in the pathogenesis of swine dysentery.</description><subject>Animals</subject><subject>Australia</subject><subject>Bacterial Outer Membrane Proteins - analysis</subject><subject>Bacterial Proteins - analysis</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>CERDO</subject><subject>DISENTERIA PORCINA</subject><subject>DYSENTERIE DU PORC</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>IDENTIFICACION</subject><subject>IDENTIFICATION</subject><subject>Microbiology</subject><subject>PORCIN</subject><subject>SWINE</subject><subject>SWINE DYSENTERY</subject><subject>TREPONEMA</subject><subject>Treponema - analysis</subject><subject>United Kingdom</subject><subject>United States</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1u1DAUha0KVKalL1CBlA0IFgH_xbE3laDiTxrBpnTRjXXj3HRcTeypnUGat6_DjNodrHzl890j-yPkFaMfGGXqIxWtrhkTzTtD3xtKpa5vjsiC6VbUvJH8GVk8Ii_ISc53tEBG0WNyzDkzSokFuf7so1vh6B2sKwh95cdxG56u3AoSuAmTzzD5GKo4VHlK4EOex6uEmxhwhGq1i_0uY5hRwJfk-QDrjGeH85T8_vrl6vJ7vfz17cflp2XtpGinuu9UB0rzhnNBGy0NbzvBERRXUvXGOdYqZkCZwQgQHQepGi5x0G1fZinEKXm7792keL_FPNnRZ4frNQSM22w1pYpqwf4LsqY1tNWmgHIPuhRzTjjYTfIjpJ1l1M7e7SzVzlKtofavd3tT1l4f-rfdiP3j0kF0yd8ccsjF65AgOJ-fuo0QrKG8cOd7boBo4bZotz-XhlGu9fyJi32IRekfj8lm5zE47H1CN9k--n-_8gG9zqbS</recordid><startdate>19900701</startdate><enddate>19900701</enddate><creator>Smith, Stuart C.</creator><creator>Roddick, Felicity</creator><creator>Ling, Soong</creator><creator>Gerraty, Norman L.</creator><creator>Coloe, Peter J.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19900701</creationdate><title>Biochemical and immunochemical characterisation of strains of Treponema hyodysenteriae</title><author>Smith, Stuart C. ; Roddick, Felicity ; Ling, Soong ; Gerraty, Norman L. ; Coloe, Peter J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-db6ba68252230584927b32ea62646d9cc17619a69f93a3b2a46524ef87d2a4433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Australia</topic><topic>Bacterial Outer Membrane Proteins - analysis</topic><topic>Bacterial Proteins - analysis</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>CERDO</topic><topic>DISENTERIA PORCINA</topic><topic>DYSENTERIE DU PORC</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>IDENTIFICACION</topic><topic>IDENTIFICATION</topic><topic>Microbiology</topic><topic>PORCIN</topic><topic>SWINE</topic><topic>SWINE DYSENTERY</topic><topic>TREPONEMA</topic><topic>Treponema - analysis</topic><topic>United Kingdom</topic><topic>United States</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith, Stuart C.</creatorcontrib><creatorcontrib>Roddick, Felicity</creatorcontrib><creatorcontrib>Ling, Soong</creatorcontrib><creatorcontrib>Gerraty, Norman L.</creatorcontrib><creatorcontrib>Coloe, Peter J.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith, Stuart C.</au><au>Roddick, Felicity</au><au>Ling, Soong</au><au>Gerraty, Norman L.</au><au>Coloe, Peter J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biochemical and immunochemical characterisation of strains of Treponema hyodysenteriae</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>1990-07-01</date><risdate>1990</risdate><volume>24</volume><issue>1</issue><spage>29</spage><epage>41</epage><pages>29-41</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>The protein composition of 18 clinical isolates of
Treponema hyodysenteriae from pigs with swine dysebtery in Australia were compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunublot analysis. Coomassie Blue stained SDS-PAGE-profiles of whole cell and outer membrane (OM) proteins demonstrated the same gel pattern among the
T. hyodysenteriae isolates, particularly the OM proteins in the molecular mass (M
r) range of 30 kDa to 40 kDa. The
T. hyodysenteriae isolates were categorised into two distinct groups (A and B) based on the strain-variability in the 37 kDa OM protein. Immunoblotting of whole cell proteins after SDS-PAGE using serum from rabbits and pigs immunised with known
T. hyodysenteriae serotypes revealed a number of common immunoreactive bands in all isolates. LPS typing of the
T. hyodysenteriae isolates by immunoblotting with the rabbit antiserum revealed one additional serotype emphasising the LPS heterogeneity among the strains isolated from geographic locations in Australia, Great Britain and the U.S.A. Immunoblotting of the OM preparations revealed several common immunoreactive polypeptides corresponding to M
r values of 34 kDa to 30 kDa among the
T. hyodysenteriae and
T. innocens isolates but a distinct 39 kDa found only in the
T. hyodysenteriae isolates. Trypsin proteolysis of intact
T. hyodysenteriae cells caused selective loss of these and other major abundant proteins identifying the location of the 39 kDa, 36 kDa,and 30 kDa proteins on the cell surface and suggesting a possible role of these proteins in the pathogenesis of swine dysentery.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2219663</pmid><doi>10.1016/0378-1135(90)90048-Z</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1135 |
| ispartof | Veterinary microbiology, 1990-07, Vol.24 (1), p.29-41 |
| issn | 0378-1135 1873-2542 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80060831 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Australia Bacterial Outer Membrane Proteins - analysis Bacterial Proteins - analysis Bacteriology Biological and medical sciences Blotting, Western CERDO DISENTERIA PORCINA DYSENTERIE DU PORC Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology IDENTIFICACION IDENTIFICATION Microbiology PORCIN SWINE SWINE DYSENTERY TREPONEMA Treponema - analysis United Kingdom United States Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies |
| title | Biochemical and immunochemical characterisation of strains of Treponema hyodysenteriae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T19%3A54%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biochemical%20and%20immunochemical%20characterisation%20of%20strains%20of%20Treponema%20hyodysenteriae&rft.jtitle=Veterinary%20microbiology&rft.au=Smith,%20Stuart%20C.&rft.date=1990-07-01&rft.volume=24&rft.issue=1&rft.spage=29&rft.epage=41&rft.pages=29-41&rft.issn=0378-1135&rft.eissn=1873-2542&rft.coden=VMICDQ&rft_id=info:doi/10.1016/0378-1135(90)90048-Z&rft_dat=%3Cproquest_cross%3E15790789%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15790789&rft_id=info:pmid/2219663&rft_els_id=037811359090048Z&rfr_iscdi=true |