The use of enzyme immunoassay (EIA) and radiobinding assay to investigate the cross-reactivity of Klebsiella antigens and HLA B27 in ankylosing spondylitis patients and healthy controls
The binding of rabbit anti-Klebsiella antibodies to tissue-typed lymphocytes obtained from 30 ankylosing spondylitis (AS) patients and 54 healthy subjects has been measured by an enzyme immunoassay method. HLA B27 positive lymphocytes obtained from either AS patients (t = 3.60; p less than 0.001) or...
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| Veröffentlicht in: | Scandinavian journal of rheumatology 1990, Vol.19 (5), p.341-349 |
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| container_end_page | 349 |
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| container_issue | 5 |
| container_start_page | 341 |
| container_title | Scandinavian journal of rheumatology |
| container_volume | 19 |
| creator | BAINES, M EBRINGER, A AVAKIAN, H SAMUEL, D JAMES, D. C. O |
| description | The binding of rabbit anti-Klebsiella antibodies to tissue-typed lymphocytes obtained from 30 ankylosing spondylitis (AS) patients and 54 healthy subjects has been measured by an enzyme immunoassay method. HLA B27 positive lymphocytes obtained from either AS patients (t = 3.60; p less than 0.001) or healthy subjects (t = 3.77; p less than 0.001) were found to bind Klebsiella antibodies to a significantly greater extent than non-B27 lymphocytes obtained from healthy controls. Absorption studies demonstrated that HLA B27 positive lymphocytes absorbed out significantly more anti-Klebsiella antibodies than HLA B27-negative lymphocytes (t = 6.76; p less than 0.005). These results are compatible with cross-reactivity or molecular mimicry between HLA B27 and epitopes on some Gram-negative bacteria such as Klebsiella. |
| doi_str_mv | 10.3109/03009749009096789 |
| format | Article |
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These results are compatible with cross-reactivity or molecular mimicry between HLA B27 and epitopes on some Gram-negative bacteria such as Klebsiella.</description><identifier>ISSN: 0300-9742</identifier><identifier>EISSN: 1502-7732</identifier><identifier>DOI: 10.3109/03009749009096789</identifier><identifier>PMID: 1699267</identifier><identifier>CODEN: SJRHAT</identifier><language>eng</language><publisher>Colchester: Taylor & Francis</publisher><subject>Antigens, Bacterial - immunology ; Biological and medical sciences ; Cross Reactions ; Diseases of the osteoarticular system ; Epitopes ; HLA-B27 Antigen - immunology ; Humans ; Immune Sera - immunology ; Immunoenzyme Techniques ; Klebsiella - immunology ; Medical sciences ; Models, Genetic ; Radioligand Assay ; Reference Values ; Spondylitis, Ankylosing - immunology</subject><ispartof>Scandinavian journal of rheumatology, 1990, Vol.19 (5), p.341-349</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4022,27921,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4616934$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1699267$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BAINES, M</creatorcontrib><creatorcontrib>EBRINGER, A</creatorcontrib><creatorcontrib>AVAKIAN, H</creatorcontrib><creatorcontrib>SAMUEL, D</creatorcontrib><creatorcontrib>JAMES, D. C. O</creatorcontrib><title>The use of enzyme immunoassay (EIA) and radiobinding assay to investigate the cross-reactivity of Klebsiella antigens and HLA B27 in ankylosing spondylitis patients and healthy controls</title><title>Scandinavian journal of rheumatology</title><addtitle>Scand J Rheumatol</addtitle><description>The binding of rabbit anti-Klebsiella antibodies to tissue-typed lymphocytes obtained from 30 ankylosing spondylitis (AS) patients and 54 healthy subjects has been measured by an enzyme immunoassay method. HLA B27 positive lymphocytes obtained from either AS patients (t = 3.60; p less than 0.001) or healthy subjects (t = 3.77; p less than 0.001) were found to bind Klebsiella antibodies to a significantly greater extent than non-B27 lymphocytes obtained from healthy controls. Absorption studies demonstrated that HLA B27 positive lymphocytes absorbed out significantly more anti-Klebsiella antibodies than HLA B27-negative lymphocytes (t = 6.76; p less than 0.005). These results are compatible with cross-reactivity or molecular mimicry between HLA B27 and epitopes on some Gram-negative bacteria such as Klebsiella.</description><subject>Antigens, Bacterial - immunology</subject><subject>Biological and medical sciences</subject><subject>Cross Reactions</subject><subject>Diseases of the osteoarticular system</subject><subject>Epitopes</subject><subject>HLA-B27 Antigen - immunology</subject><subject>Humans</subject><subject>Immune Sera - immunology</subject><subject>Immunoenzyme Techniques</subject><subject>Klebsiella - immunology</subject><subject>Medical sciences</subject><subject>Models, Genetic</subject><subject>Radioligand Assay</subject><subject>Reference Values</subject><subject>Spondylitis, Ankylosing - immunology</subject><issn>0300-9742</issn><issn>1502-7732</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMFu1DAQhi1EVZbSB-CA5ANCcAjYsROvj9uq0IqVuLTnlWNPugbHDhmnUngz3g4vu-plRr_-T580Q8hbzj4LzvQXJhjTSuoymW7VWr8gK96wulJK1C_J6tBXBahfkdeIPxljUit9Ts55q3XdqhX5e78HOiPQ1FOIf5YBqB-GOSaDaBb68eZu84ma6OhknE-dj87HR3osc6I-PgFm_2gy0FxMdkqI1QTGZv_k83LQfg_QoYcQTBEVFiL-N95uN_SqVsVR4q8lJDyocUzRLcFnj3Q02UPMR3wPJuT9Qm2KeUoB35Cz3gSEy9O-IA9fb-6vb6vtj29315ttNfJW5Upy1jQ9dzXvatM6XfNG9FBrK6Fp18r2Ta9ZB70wJQppHW862UrtOgeNZUxckA9H7zil33O5djd4tIdzIqQZd2vGGq24LOC7Ezh3A7jdOPnBTMvu9OvSvz_1Bq0J_WSi9fiMybaAQop_Pr2RPg</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>BAINES, M</creator><creator>EBRINGER, A</creator><creator>AVAKIAN, H</creator><creator>SAMUEL, D</creator><creator>JAMES, D. 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O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p167t-41055f1d21b2a6d92153fe29c4e5687cf5f90bef3a56834cd15b4649dbde5c003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Antigens, Bacterial - immunology</topic><topic>Biological and medical sciences</topic><topic>Cross Reactions</topic><topic>Diseases of the osteoarticular system</topic><topic>Epitopes</topic><topic>HLA-B27 Antigen - immunology</topic><topic>Humans</topic><topic>Immune Sera - immunology</topic><topic>Immunoenzyme Techniques</topic><topic>Klebsiella - immunology</topic><topic>Medical sciences</topic><topic>Models, Genetic</topic><topic>Radioligand Assay</topic><topic>Reference Values</topic><topic>Spondylitis, Ankylosing - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BAINES, M</creatorcontrib><creatorcontrib>EBRINGER, A</creatorcontrib><creatorcontrib>AVAKIAN, H</creatorcontrib><creatorcontrib>SAMUEL, D</creatorcontrib><creatorcontrib>JAMES, D. 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O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The use of enzyme immunoassay (EIA) and radiobinding assay to investigate the cross-reactivity of Klebsiella antigens and HLA B27 in ankylosing spondylitis patients and healthy controls</atitle><jtitle>Scandinavian journal of rheumatology</jtitle><addtitle>Scand J Rheumatol</addtitle><date>1990</date><risdate>1990</risdate><volume>19</volume><issue>5</issue><spage>341</spage><epage>349</epage><pages>341-349</pages><issn>0300-9742</issn><eissn>1502-7732</eissn><coden>SJRHAT</coden><abstract>The binding of rabbit anti-Klebsiella antibodies to tissue-typed lymphocytes obtained from 30 ankylosing spondylitis (AS) patients and 54 healthy subjects has been measured by an enzyme immunoassay method. HLA B27 positive lymphocytes obtained from either AS patients (t = 3.60; p less than 0.001) or healthy subjects (t = 3.77; p less than 0.001) were found to bind Klebsiella antibodies to a significantly greater extent than non-B27 lymphocytes obtained from healthy controls. Absorption studies demonstrated that HLA B27 positive lymphocytes absorbed out significantly more anti-Klebsiella antibodies than HLA B27-negative lymphocytes (t = 6.76; p less than 0.005). These results are compatible with cross-reactivity or molecular mimicry between HLA B27 and epitopes on some Gram-negative bacteria such as Klebsiella.</abstract><cop>Colchester</cop><pub>Taylor & Francis</pub><pmid>1699267</pmid><doi>10.3109/03009749009096789</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-9742 |
| ispartof | Scandinavian journal of rheumatology, 1990, Vol.19 (5), p.341-349 |
| issn | 0300-9742 1502-7732 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80059714 |
| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Antigens, Bacterial - immunology Biological and medical sciences Cross Reactions Diseases of the osteoarticular system Epitopes HLA-B27 Antigen - immunology Humans Immune Sera - immunology Immunoenzyme Techniques Klebsiella - immunology Medical sciences Models, Genetic Radioligand Assay Reference Values Spondylitis, Ankylosing - immunology |
| title | The use of enzyme immunoassay (EIA) and radiobinding assay to investigate the cross-reactivity of Klebsiella antigens and HLA B27 in ankylosing spondylitis patients and healthy controls |
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