Binding of feline immunodeficiency viral gag-p24 polypeptide to nonimmune Igs

Available data on the existence of lentivirus proteins with properties of unconventional Ag for B cells, have been so far restricted to human immunodeficiency virus (i.e. gp-120 of HIV-I). By using biotinylated-MAbs-anti-biotin IgG as readout system, we now report that gag-p24 antigen, either assemb...

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Veröffentlicht in:	Immunology letters 1998-06, Vol.62 (2), p.93-97
Hauptverfasser:	Amici, A, Smorlesi, A, Concetti, A, Ripani, E, Battistini, L, Venanzi, F M
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Animals Cats Gene Products, gag - immunology Immunodeficiency Virus, Feline - immunology Immunoglobulins - immunology Mice Mice, Inbred BALB C Peptides - immunology Recombinant Fusion Proteins - immunology
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creator	Amici, A Smorlesi, A Concetti, A Ripani, E Battistini, L Venanzi, F M
description	Available data on the existence of lentivirus proteins with properties of unconventional Ag for B cells, have been so far restricted to human immunodeficiency virus (i.e. gp-120 of HIV-I). By using biotinylated-MAbs-anti-biotin IgG as readout system, we now report that gag-p24 antigen, either assembled in feline immunodeficiency virus (FIV) particles or expressed as recombinant polypeptide (rec.p24) may bind to nonimmune IgGs purified from mouse or cat sera. Moreover, FACS scanning experiments are consistent with the possibility that rec.p24 interacts with surface-Ig in a sub-population (5-6%) of rodent B cells. We hypothesize that gag-p24 peptide encoded regions may bind to unconventional Ig sites or, alternatively, that they may represent 'public' epitopes for natural polyreactive antibody.
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source	MEDLINE; Elsevier ScienceDirect Journals Complete
subjects	AIDS/HIV Animals Cats Gene Products, gag - immunology Immunodeficiency Virus, Feline - immunology Immunoglobulins - immunology Mice Mice, Inbred BALB C Peptides - immunology Recombinant Fusion Proteins - immunology
title	Binding of feline immunodeficiency viral gag-p24 polypeptide to nonimmune Igs
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