Expression and purification of recombinant tick anticoagulant peptide (Y1W/D10R) double mutant secreted by Saccharomyces cerevisiae

A double mutant of tick anticoagulant peptide (TAP) was cloned as a chimeric fusion with the yeast alpha-mating factor pre-proleader peptide. Expression in yeast (Saccharomyces cerevisiae) resulted in the secretion of the TAP mutein into the culture medium. An HPLC-based assay was used to screen yea...

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Veröffentlicht in:	Protein expression and purification 1998-08, Vol.13 (3), p.291-300
Hauptverfasser:	Cook, J C, Schultz, L D, Huang, J, George, H A, Herber, W K, Ip, C, Joyce, J G, Mao, S S, Markus, H Z, Miller, W J, Sardana, M K, Lehman, E D
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Arthropod Proteins Base Sequence Chromatography, High Pressure Liquid Chromatography, Ion Exchange Cloning, Molecular DNA, Recombinant Genetic Vectors Mating Factor Molecular Sequence Data Peptides - genetics Peptides - isolation & purification Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - isolation & purification Saccharomyces cerevisiae - genetics Ticks - genetics
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