Activation of the 20S Proteasome of Xenopus Oocytes by Cardiolipin: Blockage of the Activation of Trypsin-like Activity by the Substrate
The effects of an activator, cardiolipin, on the three peptidase activities of the 20S proteasome of Xenopus oocytes were examined. The trypsin-like activity was activated when the enzyme was treated with cardiolipin before the addition of the substrate, but there was no appreciable activation when...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1998-06, Vol.62 (6), p.1264-1266 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | YAMADA, Shinpei SATO, Kentaro URITANI, Masahiro TOKUMOTO, Toshinobu ISHIKAWA, Katsutoshi |
| description | The effects of an activator, cardiolipin, on the three peptidase activities of the 20S proteasome of Xenopus oocytes were examined. The trypsin-like activity was activated when the enzyme was treated with cardiolipin before the addition of the substrate, but there was no appreciable activation when cardiolipin was added concomitantly with the substrate. On the other hand, the chymotrypsin-like peptidase and peptidylglutamylpeptide hydrolase (PGPH) were activated regardless of the sequence of addition. When very low concentrations of the substrate (e.g. 0.1-0.5 μM; about 1/100 of the K
m
) were used, cardiolipin strongly activated trypsin-like peptidase by the simultaneous addition but not after substrate addition. These results suggest that the trypsin-type substrate produces a conformational change in the enzyme in a concentration-dependent manner which makes the activator sites inaccessible to cardiolipin. |
| doi_str_mv | 10.1271/bbb.62.1264 |
| format | Article |
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m
) were used, cardiolipin strongly activated trypsin-like peptidase by the simultaneous addition but not after substrate addition. These results suggest that the trypsin-type substrate produces a conformational change in the enzyme in a concentration-dependent manner which makes the activator sites inaccessible to cardiolipin.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.62.1264</identifier><identifier>PMID: 9692214</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>20S proteasome ; activator cardiolipin ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Cardiolipins - pharmacology ; conformational change ; Endopeptidases - metabolism ; Enzyme Activation ; Enzymes and enzyme inhibitors ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Hydrolases ; Multienzyme Complexes - metabolism ; Oocytes - drug effects ; Oocytes - enzymology ; Substrate Specificity ; Trypsin - metabolism ; trypsin-like activity ; Xenopus ; Xenopus laevis ; Xenopus oocytes</subject><ispartof>Bioscience, biotechnology, and biochemistry, 1998-06, Vol.62 (6), p.1264-1266</ispartof><rights>1998 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 1998</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-310a4ac9d3cb7fdaaedd655fcce6a63cc81ef0a80b966ce9fe6a3e3b70a5b43b3</citedby><cites>FETCH-LOGICAL-c517t-310a4ac9d3cb7fdaaedd655fcce6a63cc81ef0a80b966ce9fe6a3e3b70a5b43b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2438902$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9692214$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YAMADA, Shinpei</creatorcontrib><creatorcontrib>SATO, Kentaro</creatorcontrib><creatorcontrib>URITANI, Masahiro</creatorcontrib><creatorcontrib>TOKUMOTO, Toshinobu</creatorcontrib><creatorcontrib>ISHIKAWA, Katsutoshi</creatorcontrib><title>Activation of the 20S Proteasome of Xenopus Oocytes by Cardiolipin: Blockage of the Activation of Trypsin-like Activity by the Substrate</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>The effects of an activator, cardiolipin, on the three peptidase activities of the 20S proteasome of Xenopus oocytes were examined. The trypsin-like activity was activated when the enzyme was treated with cardiolipin before the addition of the substrate, but there was no appreciable activation when cardiolipin was added concomitantly with the substrate. On the other hand, the chymotrypsin-like peptidase and peptidylglutamylpeptide hydrolase (PGPH) were activated regardless of the sequence of addition. When very low concentrations of the substrate (e.g. 0.1-0.5 μM; about 1/100 of the K
m
) were used, cardiolipin strongly activated trypsin-like peptidase by the simultaneous addition but not after substrate addition. These results suggest that the trypsin-type substrate produces a conformational change in the enzyme in a concentration-dependent manner which makes the activator sites inaccessible to cardiolipin.</description><subject>20S proteasome</subject><subject>activator cardiolipin</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cardiolipins - pharmacology</subject><subject>conformational change</subject><subject>Endopeptidases - metabolism</subject><subject>Enzyme Activation</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolases</subject><subject>Multienzyme Complexes - metabolism</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - enzymology</subject><subject>Substrate Specificity</subject><subject>Trypsin - metabolism</subject><subject>trypsin-like activity</subject><subject>Xenopus</subject><subject>Xenopus laevis</subject><subject>Xenopus oocytes</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9r1jAcxoM45uv05FnoQbxIZ9KkSeNtvvhjMJiwCd7CN2mqcWlTk1Tpf-CfvZa3GwgDTwnP88knhwehFwSfkkqQt1rrU14td84eoR2hTJRcMvEY7bAkvGxYTZ6gpyn9xHgJanKMjiWXVUXYDv09M9n9huzCUISuyD9sUeGr4ksM2UIKvV3Tb3YI45SKy2DmbFOh52IPsXXBu9EN74r3Ppgb-G7vDP86r-M8JjeU3t1slcvz6ljRq0mnHCHbZ-ioA5_s8-08QV8_frjefy4vLj-d788uSlMTkUtKMDAwsqVGi64FsG3L67ozxnLg1JiG2A5Dg7Xk3FjZLTG1VAsMtWZU0xP0-uAdY_g12ZRV75Kx3sNgw5RUgzETXPL_gkSQWlBBFvDNATQxpBRtp8boeoizIlitA6llIMUrtQ600C837aR7296z2yJL_2rrIRnwXYTBuHSPVYw2ElcLxg-YG7oQe_gTom9VhtmHePeGPvT_LfLnrYE</recordid><startdate>19980601</startdate><enddate>19980601</enddate><creator>YAMADA, Shinpei</creator><creator>SATO, Kentaro</creator><creator>URITANI, Masahiro</creator><creator>TOKUMOTO, Toshinobu</creator><creator>ISHIKAWA, Katsutoshi</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>19980601</creationdate><title>Activation of the 20S Proteasome of Xenopus Oocytes by Cardiolipin: Blockage of the Activation of Trypsin-like Activity by the Substrate</title><author>YAMADA, Shinpei ; SATO, Kentaro ; URITANI, Masahiro ; TOKUMOTO, Toshinobu ; ISHIKAWA, Katsutoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-310a4ac9d3cb7fdaaedd655fcce6a63cc81ef0a80b966ce9fe6a3e3b70a5b43b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>20S proteasome</topic><topic>activator cardiolipin</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cardiolipins - pharmacology</topic><topic>conformational change</topic><topic>Endopeptidases - metabolism</topic><topic>Enzyme Activation</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrolases</topic><topic>Multienzyme Complexes - metabolism</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - enzymology</topic><topic>Substrate Specificity</topic><topic>Trypsin - metabolism</topic><topic>trypsin-like activity</topic><topic>Xenopus</topic><topic>Xenopus laevis</topic><topic>Xenopus oocytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YAMADA, Shinpei</creatorcontrib><creatorcontrib>SATO, Kentaro</creatorcontrib><creatorcontrib>URITANI, Masahiro</creatorcontrib><creatorcontrib>TOKUMOTO, Toshinobu</creatorcontrib><creatorcontrib>ISHIKAWA, Katsutoshi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YAMADA, Shinpei</au><au>SATO, Kentaro</au><au>URITANI, Masahiro</au><au>TOKUMOTO, Toshinobu</au><au>ISHIKAWA, Katsutoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation of the 20S Proteasome of Xenopus Oocytes by Cardiolipin: Blockage of the Activation of Trypsin-like Activity by the Substrate</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>1998-06-01</date><risdate>1998</risdate><volume>62</volume><issue>6</issue><spage>1264</spage><epage>1266</epage><pages>1264-1266</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>The effects of an activator, cardiolipin, on the three peptidase activities of the 20S proteasome of Xenopus oocytes were examined. The trypsin-like activity was activated when the enzyme was treated with cardiolipin before the addition of the substrate, but there was no appreciable activation when cardiolipin was added concomitantly with the substrate. On the other hand, the chymotrypsin-like peptidase and peptidylglutamylpeptide hydrolase (PGPH) were activated regardless of the sequence of addition. When very low concentrations of the substrate (e.g. 0.1-0.5 μM; about 1/100 of the K
m
) were used, cardiolipin strongly activated trypsin-like peptidase by the simultaneous addition but not after substrate addition. These results suggest that the trypsin-type substrate produces a conformational change in the enzyme in a concentration-dependent manner which makes the activator sites inaccessible to cardiolipin.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>9692214</pmid><doi>10.1271/bbb.62.1264</doi><tpages>3</tpages></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 1998-06, Vol.62 (6), p.1264-1266 |
| issn | 0916-8451 1347-6947 |
| language | eng |
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| source | J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Open Access Titles of Japan; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | 20S proteasome activator cardiolipin Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cardiolipins - pharmacology conformational change Endopeptidases - metabolism Enzyme Activation Enzymes and enzyme inhibitors Freshwater Fundamental and applied biological sciences. Psychology Hydrolases Multienzyme Complexes - metabolism Oocytes - drug effects Oocytes - enzymology Substrate Specificity Trypsin - metabolism trypsin-like activity Xenopus Xenopus laevis Xenopus oocytes |
| title | Activation of the 20S Proteasome of Xenopus Oocytes by Cardiolipin: Blockage of the Activation of Trypsin-like Activity by the Substrate |
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