Somatic antigens of pseudomonads: Structure of the O-specific polysaccharide of Pseudomonas fluorescens biovar B, strain IMV 247
The O-specific polysaccharide of Pseudomonas fluorescens biovar B, strain IMV 247, was studied by acid hydrolysis, GLC–MS and 1 H and 13 C NMR spectroscopy, including 1D and 2D NOE, 2D hybrid TOCSY and ROESY (TORO), and 2D H-detected heteronuclear multiple-bond correlation (HMBC) experiments. The po...
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| Veröffentlicht in: | Carbohydrate research 1998, Vol.306 (1), p.297-303 |
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| container_title | Carbohydrate research |
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| creator | Shashkov, Alexander S Paramonov, Nikolay A Veremeychenko, Stanislav P Grosskurth, Horst Zdorovenko, Galina M Knirel, Yuriy A Kochetkov, Nikolay K |
| description | The O-specific polysaccharide of
Pseudomonas fluorescens biovar B, strain IMV 247, was studied by acid hydrolysis, GLC–MS and
1
H
and
13
C
NMR spectroscopy, including 1D and 2D NOE, 2D hybrid TOCSY and ROESY (TORO), and 2D H-detected heteronuclear multiple-bond correlation (HMBC) experiments. The polysaccharide was found to contain
l-rhamnose, 3,6-dideoxy-3-[(
S)-3-hydroxybutyramido]-
d-glucose (
d-Qui3NHb), 2-acetamido-2,4,6-trideoxy-4-[(
S)-3-hydroxybutyramido-
d-glucose (
d-QuiNAc4NHb) and 2-acetamido-2-deoxy-
d-galacturonic acid (
d-GalNAcA). Partial acid hydrolysis of the polysaccharide resulted in a non-reducing GalNAcA→QuiNAc4NHb disaccharide with the 3-hydroxybutyryl group glycosylated intramolecularly by the QuiN4N residue. The following structure of the tetrasaccharide repeating unit of the polysaccharide was established: →4)-
α-
d-Gal
pNAcA-(1→3)-
α-
d-Qui
pNAc4NHb-(1→2)-
β-
d-Qui
p3NHb-(1→2)-
α-
l-Rha
p-(1→. |
| doi_str_mv | 10.1016/S0008-6215(97)10048-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80045037</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0008621597100489</els_id><sourcerecordid>80045037</sourcerecordid><originalsourceid>FETCH-LOGICAL-c360t-a7b9acceaf1005af4a055bb0014d1d5508d13b035ae401cbfe2543ab020217a33</originalsourceid><addsrcrecordid>eNqFkEtr3TAQhUVoSG8ePyGgVWghbka25Ec3oQ19BBJSuE3oTsjSOFGxLVeSA9nlp1f3wd1mJcQ5Z87MR8gpg08MWHmxBIA6K3MmPjTVRwbA66zZIwtWV0XG8_LPO7LYWd6TwxD-pi-UVXlADpqyYVzwBXldukFFq6kao33EMVDX0SngbNzgRmXCZ7qMftZx9riS4hPSuyxMqG2XUpPrX4LS-kl5a9aGX7tsoF0_O49Br8a21j0rT7-e0xC9siO9vn2gOa-OyX6n-oAn2_eI3H__9vvqZ3Zz9-P66stNposSYqaqtkk9qLp0qVAdVyBE2wIwbpgRAmrDihYKoZAD022HueCFaiGHnFWqKI7I2Wbu5N2_GUOUg02b9b0a0c1B1gmggKJKRrExau9C8NjJydtB-RfJQK7IyzV5ucIqm0quycsm5U63BXM7oNmltqiTfrnRMV35bNHLoC2OGo31qKM0zr7R8B8rWJQ6</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80045037</pqid></control><display><type>article</type><title>Somatic antigens of pseudomonads: Structure of the O-specific polysaccharide of Pseudomonas fluorescens biovar B, strain IMV 247</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Shashkov, Alexander S ; Paramonov, Nikolay A ; Veremeychenko, Stanislav P ; Grosskurth, Horst ; Zdorovenko, Galina M ; Knirel, Yuriy A ; Kochetkov, Nikolay K</creator><creatorcontrib>Shashkov, Alexander S ; Paramonov, Nikolay A ; Veremeychenko, Stanislav P ; Grosskurth, Horst ; Zdorovenko, Galina M ; Knirel, Yuriy A ; Kochetkov, Nikolay K</creatorcontrib><description>The O-specific polysaccharide of
Pseudomonas fluorescens biovar B, strain IMV 247, was studied by acid hydrolysis, GLC–MS and
1
H
and
13
C
NMR spectroscopy, including 1D and 2D NOE, 2D hybrid TOCSY and ROESY (TORO), and 2D H-detected heteronuclear multiple-bond correlation (HMBC) experiments. The polysaccharide was found to contain
l-rhamnose, 3,6-dideoxy-3-[(
S)-3-hydroxybutyramido]-
d-glucose (
d-Qui3NHb), 2-acetamido-2,4,6-trideoxy-4-[(
S)-3-hydroxybutyramido-
d-glucose (
d-QuiNAc4NHb) and 2-acetamido-2-deoxy-
d-galacturonic acid (
d-GalNAcA). Partial acid hydrolysis of the polysaccharide resulted in a non-reducing GalNAcA→QuiNAc4NHb disaccharide with the 3-hydroxybutyryl group glycosylated intramolecularly by the QuiN4N residue. The following structure of the tetrasaccharide repeating unit of the polysaccharide was established: →4)-
α-
d-Gal
pNAcA-(1→3)-
α-
d-Qui
pNAc4NHb-(1→2)-
β-
d-Qui
p3NHb-(1→2)-
α-
l-Rha
p-(1→.</description><identifier>ISSN: 0008-6215</identifier><identifier>EISSN: 1873-426X</identifier><identifier>DOI: 10.1016/S0008-6215(97)10048-9</identifier><identifier>PMID: 9691454</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>( S)-3-Hydroxybutyrate ; 2,4-Diamino-2,4,6-trideoxy- d-glucose ; 2-Amino-2-deoxy- d-galacturonic acid ; 3-Amino-3,6-dideoxy- d-glucose ; Bacterial polysaccharide ; Carbohydrate Conformation ; Carbohydrate Sequence ; Gas Chromatography-Mass Spectrometry ; Lipopolysaccharide ; Magnetic Resonance Spectroscopy ; Molecular Sequence Data ; O Antigens - analysis ; Oligosaccharides - chemistry ; Pseudomonas fluorescens ; Pseudomonas fluorescens - immunology</subject><ispartof>Carbohydrate research, 1998, Vol.306 (1), p.297-303</ispartof><rights>1998 Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-a7b9acceaf1005af4a055bb0014d1d5508d13b035ae401cbfe2543ab020217a33</citedby><cites>FETCH-LOGICAL-c360t-a7b9acceaf1005af4a055bb0014d1d5508d13b035ae401cbfe2543ab020217a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0008621597100489$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,4009,27902,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9691454$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shashkov, Alexander S</creatorcontrib><creatorcontrib>Paramonov, Nikolay A</creatorcontrib><creatorcontrib>Veremeychenko, Stanislav P</creatorcontrib><creatorcontrib>Grosskurth, Horst</creatorcontrib><creatorcontrib>Zdorovenko, Galina M</creatorcontrib><creatorcontrib>Knirel, Yuriy A</creatorcontrib><creatorcontrib>Kochetkov, Nikolay K</creatorcontrib><title>Somatic antigens of pseudomonads: Structure of the O-specific polysaccharide of Pseudomonas fluorescens biovar B, strain IMV 247</title><title>Carbohydrate research</title><addtitle>Carbohydr Res</addtitle><description>The O-specific polysaccharide of
Pseudomonas fluorescens biovar B, strain IMV 247, was studied by acid hydrolysis, GLC–MS and
1
H
and
13
C
NMR spectroscopy, including 1D and 2D NOE, 2D hybrid TOCSY and ROESY (TORO), and 2D H-detected heteronuclear multiple-bond correlation (HMBC) experiments. The polysaccharide was found to contain
l-rhamnose, 3,6-dideoxy-3-[(
S)-3-hydroxybutyramido]-
d-glucose (
d-Qui3NHb), 2-acetamido-2,4,6-trideoxy-4-[(
S)-3-hydroxybutyramido-
d-glucose (
d-QuiNAc4NHb) and 2-acetamido-2-deoxy-
d-galacturonic acid (
d-GalNAcA). Partial acid hydrolysis of the polysaccharide resulted in a non-reducing GalNAcA→QuiNAc4NHb disaccharide with the 3-hydroxybutyryl group glycosylated intramolecularly by the QuiN4N residue. The following structure of the tetrasaccharide repeating unit of the polysaccharide was established: →4)-
α-
d-Gal
pNAcA-(1→3)-
α-
d-Qui
pNAc4NHb-(1→2)-
β-
d-Qui
p3NHb-(1→2)-
α-
l-Rha
p-(1→.</description><subject>( S)-3-Hydroxybutyrate</subject><subject>2,4-Diamino-2,4,6-trideoxy- d-glucose</subject><subject>2-Amino-2-deoxy- d-galacturonic acid</subject><subject>3-Amino-3,6-dideoxy- d-glucose</subject><subject>Bacterial polysaccharide</subject><subject>Carbohydrate Conformation</subject><subject>Carbohydrate Sequence</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Lipopolysaccharide</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Molecular Sequence Data</subject><subject>O Antigens - analysis</subject><subject>Oligosaccharides - chemistry</subject><subject>Pseudomonas fluorescens</subject><subject>Pseudomonas fluorescens - immunology</subject><issn>0008-6215</issn><issn>1873-426X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtr3TAQhUVoSG8ePyGgVWghbka25Ec3oQ19BBJSuE3oTsjSOFGxLVeSA9nlp1f3wd1mJcQ5Z87MR8gpg08MWHmxBIA6K3MmPjTVRwbA66zZIwtWV0XG8_LPO7LYWd6TwxD-pi-UVXlADpqyYVzwBXldukFFq6kao33EMVDX0SngbNzgRmXCZ7qMftZx9riS4hPSuyxMqG2XUpPrX4LS-kl5a9aGX7tsoF0_O49Br8a21j0rT7-e0xC9siO9vn2gOa-OyX6n-oAn2_eI3H__9vvqZ3Zz9-P66stNposSYqaqtkk9qLp0qVAdVyBE2wIwbpgRAmrDihYKoZAD022HueCFaiGHnFWqKI7I2Wbu5N2_GUOUg02b9b0a0c1B1gmggKJKRrExau9C8NjJydtB-RfJQK7IyzV5ucIqm0quycsm5U63BXM7oNmltqiTfrnRMV35bNHLoC2OGo31qKM0zr7R8B8rWJQ6</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>Shashkov, Alexander S</creator><creator>Paramonov, Nikolay A</creator><creator>Veremeychenko, Stanislav P</creator><creator>Grosskurth, Horst</creator><creator>Zdorovenko, Galina M</creator><creator>Knirel, Yuriy A</creator><creator>Kochetkov, Nikolay K</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1998</creationdate><title>Somatic antigens of pseudomonads: Structure of the O-specific polysaccharide of Pseudomonas fluorescens biovar B, strain IMV 247</title><author>Shashkov, Alexander S ; Paramonov, Nikolay A ; Veremeychenko, Stanislav P ; Grosskurth, Horst ; Zdorovenko, Galina M ; Knirel, Yuriy A ; Kochetkov, Nikolay K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-a7b9acceaf1005af4a055bb0014d1d5508d13b035ae401cbfe2543ab020217a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>( S)-3-Hydroxybutyrate</topic><topic>2,4-Diamino-2,4,6-trideoxy- d-glucose</topic><topic>2-Amino-2-deoxy- d-galacturonic acid</topic><topic>3-Amino-3,6-dideoxy- d-glucose</topic><topic>Bacterial polysaccharide</topic><topic>Carbohydrate Conformation</topic><topic>Carbohydrate Sequence</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Lipopolysaccharide</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Molecular Sequence Data</topic><topic>O Antigens - analysis</topic><topic>Oligosaccharides - chemistry</topic><topic>Pseudomonas fluorescens</topic><topic>Pseudomonas fluorescens - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shashkov, Alexander S</creatorcontrib><creatorcontrib>Paramonov, Nikolay A</creatorcontrib><creatorcontrib>Veremeychenko, Stanislav P</creatorcontrib><creatorcontrib>Grosskurth, Horst</creatorcontrib><creatorcontrib>Zdorovenko, Galina M</creatorcontrib><creatorcontrib>Knirel, Yuriy A</creatorcontrib><creatorcontrib>Kochetkov, Nikolay K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shashkov, Alexander S</au><au>Paramonov, Nikolay A</au><au>Veremeychenko, Stanislav P</au><au>Grosskurth, Horst</au><au>Zdorovenko, Galina M</au><au>Knirel, Yuriy A</au><au>Kochetkov, Nikolay K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Somatic antigens of pseudomonads: Structure of the O-specific polysaccharide of Pseudomonas fluorescens biovar B, strain IMV 247</atitle><jtitle>Carbohydrate research</jtitle><addtitle>Carbohydr Res</addtitle><date>1998</date><risdate>1998</risdate><volume>306</volume><issue>1</issue><spage>297</spage><epage>303</epage><pages>297-303</pages><issn>0008-6215</issn><eissn>1873-426X</eissn><abstract>The O-specific polysaccharide of
Pseudomonas fluorescens biovar B, strain IMV 247, was studied by acid hydrolysis, GLC–MS and
1
H
and
13
C
NMR spectroscopy, including 1D and 2D NOE, 2D hybrid TOCSY and ROESY (TORO), and 2D H-detected heteronuclear multiple-bond correlation (HMBC) experiments. The polysaccharide was found to contain
l-rhamnose, 3,6-dideoxy-3-[(
S)-3-hydroxybutyramido]-
d-glucose (
d-Qui3NHb), 2-acetamido-2,4,6-trideoxy-4-[(
S)-3-hydroxybutyramido-
d-glucose (
d-QuiNAc4NHb) and 2-acetamido-2-deoxy-
d-galacturonic acid (
d-GalNAcA). Partial acid hydrolysis of the polysaccharide resulted in a non-reducing GalNAcA→QuiNAc4NHb disaccharide with the 3-hydroxybutyryl group glycosylated intramolecularly by the QuiN4N residue. The following structure of the tetrasaccharide repeating unit of the polysaccharide was established: →4)-
α-
d-Gal
pNAcA-(1→3)-
α-
d-Qui
pNAc4NHb-(1→2)-
β-
d-Qui
p3NHb-(1→2)-
α-
l-Rha
p-(1→.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>9691454</pmid><doi>10.1016/S0008-6215(97)10048-9</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0008-6215 |
| ispartof | Carbohydrate research, 1998, Vol.306 (1), p.297-303 |
| issn | 0008-6215 1873-426X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80045037 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | ( S)-3-Hydroxybutyrate 2,4-Diamino-2,4,6-trideoxy- d-glucose 2-Amino-2-deoxy- d-galacturonic acid 3-Amino-3,6-dideoxy- d-glucose Bacterial polysaccharide Carbohydrate Conformation Carbohydrate Sequence Gas Chromatography-Mass Spectrometry Lipopolysaccharide Magnetic Resonance Spectroscopy Molecular Sequence Data O Antigens - analysis Oligosaccharides - chemistry Pseudomonas fluorescens Pseudomonas fluorescens - immunology |
| title | Somatic antigens of pseudomonads: Structure of the O-specific polysaccharide of Pseudomonas fluorescens biovar B, strain IMV 247 |
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