Rapid effects of corticosteroids on cytosolic protein kinase C and intracellular calcium concentration in human distal colon
Recent studies from our laboratory have reported rapid (
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| Veröffentlicht in: | Molecular and cellular endocrinology 1998-03, Vol.138 (1), p.71-79 |
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| container_title | Molecular and cellular endocrinology |
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| creator | Doolan, Christina M O' Sullivan, Gerald C Harvey, Brian J |
| description | Recent studies from our laboratory have reported rapid ( |
| doi_str_mv | 10.1016/S0303-7207(98)00020-3 |
| format | Article |
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+–H
+ exchange, protein kinase C (PKC) activity and PKC-sensitive Ca
2+ entry by mineralocorticoids in mammalian distal colonic epithelium. Previous studies from other laboratories have described stimulation of the Na
+–H
+ exchanger by PKC activation. Here a rapid non-genomic effect of aldosterone on PKC activity and intracellular free calcium [Ca
2+]
i is demonstrated in human distal colonic epithelium. Rapid activation (after 15 min incubation) of basal PKC activity was observed in cytosolic fractions of human colonic epithelium by aldosterone, fludrocortisone and deoxycorticosterone acetate (DOCA). PKC activation was inhibited by the specific PKC inhibitor bisindolylmaleimide (GF109203X). The glucocorticoid hydrocortisone failed to activate PKC activity. Aldosterone induced a rapid increase in [Ca
2+]
i in isolated human colonic crypts. This stimulatory effect on [Ca
2+]
i was inhibited by the PKC inhibitor chelerythrine chloride. Hydrocortisone and dexamethasone similarly failed to increase [Ca
2+]
i. These results indicate that intracellular signalling for aldosterone involves changes in [Ca
2+]
i via activation of PKC. Since stimulation of PKC activity and increase in [Ca
2+]
i are apparent at normal circulating levels of aldosterone, our findings may have important physiological implications and prompt a reassessment of mineralocorticoid effects on electrolyte homeostasis.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/S0303-7207(98)00020-3</identifier><identifier>PMID: 9685216</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Adrenal Cortex Hormones - pharmacology ; Aldosterone - pharmacology ; Calcium - metabolism ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Colon - metabolism ; Cytosol - enzymology ; Desoxycorticosterone - pharmacology ; Enzyme Activation ; Enzyme Inhibitors - pharmacology ; Fludrocortisone - pharmacology ; Human colonic crypts ; Humans ; Hydrocortisone - pharmacology ; In Vitro Techniques ; Indoles - pharmacology ; Intestinal Mucosa - cytology ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - metabolism ; Intracellular calcium ; Kinetics ; Maleimides - pharmacology ; Mineralocorticoids ; Mineralocorticoids - pharmacology ; Mineralocorticoids - physiology ; Non-genomic steroid action ; Protein Kinase C - metabolism ; Time Factors</subject><ispartof>Molecular and cellular endocrinology, 1998-03, Vol.138 (1), p.71-79</ispartof><rights>1998 Elsevier Science Ireland Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-9e2ad1f42910e9c003b13e1606b1d4897f92366040cc8c35a90d9fe97bb7104c3</citedby><cites>FETCH-LOGICAL-c426t-9e2ad1f42910e9c003b13e1606b1d4897f92366040cc8c35a90d9fe97bb7104c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0303-7207(98)00020-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9685216$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Doolan, Christina M</creatorcontrib><creatorcontrib>O' Sullivan, Gerald C</creatorcontrib><creatorcontrib>Harvey, Brian J</creatorcontrib><title>Rapid effects of corticosteroids on cytosolic protein kinase C and intracellular calcium concentration in human distal colon</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>Recent studies from our laboratory have reported rapid (<1 min) non-genomic activation of potassium recycling, Na
+–H
+ exchange, protein kinase C (PKC) activity and PKC-sensitive Ca
2+ entry by mineralocorticoids in mammalian distal colonic epithelium. Previous studies from other laboratories have described stimulation of the Na
+–H
+ exchanger by PKC activation. Here a rapid non-genomic effect of aldosterone on PKC activity and intracellular free calcium [Ca
2+]
i is demonstrated in human distal colonic epithelium. Rapid activation (after 15 min incubation) of basal PKC activity was observed in cytosolic fractions of human colonic epithelium by aldosterone, fludrocortisone and deoxycorticosterone acetate (DOCA). PKC activation was inhibited by the specific PKC inhibitor bisindolylmaleimide (GF109203X). The glucocorticoid hydrocortisone failed to activate PKC activity. Aldosterone induced a rapid increase in [Ca
2+]
i in isolated human colonic crypts. This stimulatory effect on [Ca
2+]
i was inhibited by the PKC inhibitor chelerythrine chloride. Hydrocortisone and dexamethasone similarly failed to increase [Ca
2+]
i. These results indicate that intracellular signalling for aldosterone involves changes in [Ca
2+]
i via activation of PKC. Since stimulation of PKC activity and increase in [Ca
2+]
i are apparent at normal circulating levels of aldosterone, our findings may have important physiological implications and prompt a reassessment of mineralocorticoid effects on electrolyte homeostasis.</description><subject>Adrenal Cortex Hormones - pharmacology</subject><subject>Aldosterone - pharmacology</subject><subject>Calcium - metabolism</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Colon - metabolism</subject><subject>Cytosol - enzymology</subject><subject>Desoxycorticosterone - pharmacology</subject><subject>Enzyme Activation</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Fludrocortisone - pharmacology</subject><subject>Human colonic crypts</subject><subject>Humans</subject><subject>Hydrocortisone - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Indoles - pharmacology</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intracellular calcium</subject><subject>Kinetics</subject><subject>Maleimides - pharmacology</subject><subject>Mineralocorticoids</subject><subject>Mineralocorticoids - pharmacology</subject><subject>Mineralocorticoids - physiology</subject><subject>Non-genomic steroid action</subject><subject>Protein Kinase C - metabolism</subject><subject>Time Factors</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1rFTEUhoNU2mv1JxSyKnUxepLMR7KSclFbKAha1yGTnMHoTHJNMkKhP97c3ku3XQXyfpxzHkIuGHxgwPqPP0CAaAYOw5WS7wGAQyNekQ2TA28kdMMJ2TxbzsibnH9X09BxeUpOVS87zvoNefxudt5RnCa0JdM4URtT8Tbmgil6V78CtQ8l5jh7S3cpFvSB_vHBZKRbaoKjPpRkLM7zOptErZmtX5faEyzuleJrRc38WhcTqPO5mLmqcwxvyevJzBnfHd9z8vPL5_vtTXP37evt9vqusS3vS6OQG8emlisGqCyAGJlA1kM_MtdKNUyKi76HFqyVVnRGgVMTqmEcBwatFefk8tBb1_-7Yi568Xm_sAkY16wlQNsK3lVjdzDaFHNOOOld8otJD5qB3lPXT9T1HqlWUj9R16LmLo4D1nFB95w6Yq76p4OO9cp_HpPO1mPl43yq3LWL_oUJ_wFniZNs</recordid><startdate>19980316</startdate><enddate>19980316</enddate><creator>Doolan, Christina M</creator><creator>O' Sullivan, Gerald C</creator><creator>Harvey, Brian J</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980316</creationdate><title>Rapid effects of corticosteroids on cytosolic protein kinase C and intracellular calcium concentration in human distal colon</title><author>Doolan, Christina M ; O' Sullivan, Gerald C ; Harvey, Brian J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-9e2ad1f42910e9c003b13e1606b1d4897f92366040cc8c35a90d9fe97bb7104c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adrenal Cortex Hormones - pharmacology</topic><topic>Aldosterone - pharmacology</topic><topic>Calcium - metabolism</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Colon - metabolism</topic><topic>Cytosol - enzymology</topic><topic>Desoxycorticosterone - pharmacology</topic><topic>Enzyme Activation</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Fludrocortisone - pharmacology</topic><topic>Human colonic crypts</topic><topic>Humans</topic><topic>Hydrocortisone - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Indoles - pharmacology</topic><topic>Intestinal Mucosa - cytology</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intracellular calcium</topic><topic>Kinetics</topic><topic>Maleimides - pharmacology</topic><topic>Mineralocorticoids</topic><topic>Mineralocorticoids - pharmacology</topic><topic>Mineralocorticoids - physiology</topic><topic>Non-genomic steroid action</topic><topic>Protein Kinase C - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Doolan, Christina M</creatorcontrib><creatorcontrib>O' Sullivan, Gerald C</creatorcontrib><creatorcontrib>Harvey, Brian J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Doolan, Christina M</au><au>O' Sullivan, Gerald C</au><au>Harvey, Brian J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid effects of corticosteroids on cytosolic protein kinase C and intracellular calcium concentration in human distal colon</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>1998-03-16</date><risdate>1998</risdate><volume>138</volume><issue>1</issue><spage>71</spage><epage>79</epage><pages>71-79</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><abstract>Recent studies from our laboratory have reported rapid (<1 min) non-genomic activation of potassium recycling, Na
+–H
+ exchange, protein kinase C (PKC) activity and PKC-sensitive Ca
2+ entry by mineralocorticoids in mammalian distal colonic epithelium. Previous studies from other laboratories have described stimulation of the Na
+–H
+ exchanger by PKC activation. Here a rapid non-genomic effect of aldosterone on PKC activity and intracellular free calcium [Ca
2+]
i is demonstrated in human distal colonic epithelium. Rapid activation (after 15 min incubation) of basal PKC activity was observed in cytosolic fractions of human colonic epithelium by aldosterone, fludrocortisone and deoxycorticosterone acetate (DOCA). PKC activation was inhibited by the specific PKC inhibitor bisindolylmaleimide (GF109203X). The glucocorticoid hydrocortisone failed to activate PKC activity. Aldosterone induced a rapid increase in [Ca
2+]
i in isolated human colonic crypts. This stimulatory effect on [Ca
2+]
i was inhibited by the PKC inhibitor chelerythrine chloride. Hydrocortisone and dexamethasone similarly failed to increase [Ca
2+]
i. These results indicate that intracellular signalling for aldosterone involves changes in [Ca
2+]
i via activation of PKC. Since stimulation of PKC activity and increase in [Ca
2+]
i are apparent at normal circulating levels of aldosterone, our findings may have important physiological implications and prompt a reassessment of mineralocorticoid effects on electrolyte homeostasis.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>9685216</pmid><doi>10.1016/S0303-7207(98)00020-3</doi><tpages>9</tpages></addata></record> |
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| issn | 0303-7207 1872-8057 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adrenal Cortex Hormones - pharmacology Aldosterone - pharmacology Calcium - metabolism Cell Membrane - drug effects Cell Membrane - metabolism Colon - metabolism Cytosol - enzymology Desoxycorticosterone - pharmacology Enzyme Activation Enzyme Inhibitors - pharmacology Fludrocortisone - pharmacology Human colonic crypts Humans Hydrocortisone - pharmacology In Vitro Techniques Indoles - pharmacology Intestinal Mucosa - cytology Intestinal Mucosa - drug effects Intestinal Mucosa - metabolism Intracellular calcium Kinetics Maleimides - pharmacology Mineralocorticoids Mineralocorticoids - pharmacology Mineralocorticoids - physiology Non-genomic steroid action Protein Kinase C - metabolism Time Factors |
| title | Rapid effects of corticosteroids on cytosolic protein kinase C and intracellular calcium concentration in human distal colon |
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