Auxin-binding-protein antibodies and peptides influence stomatal opening and alter cytoplasmic pH
Previous work has shown that stomatal opening induced by indole-3-acetic acid (IAA) in epidermal strips of the orchid Paphiopedilum tonsum L. is preceded by a reduction in cytoplasmic pH (pHi) of the guard cells. We now report that Fab fragments of an auxin-agonist antibody (D16), directed against a...
Gespeichert in:
| Veröffentlicht in: | Planta 1998-08, Vol.205 (4), p.581-586 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 586 |
|---|---|
| container_issue | 4 |
| container_start_page | 581 |
| container_title | Planta |
| container_volume | 205 |
| creator | Gehring, C.A McConchie, R.M Venis, M.A Parish, R.W |
| description | Previous work has shown that stomatal opening induced by indole-3-acetic acid (IAA) in epidermal strips of the orchid Paphiopedilum tonsum L. is preceded by a reduction in cytoplasmic pH (pHi) of the guard cells. We now report that Fab fragments of an auxin-agonist antibody (D16), directed against a putative auxin-binding domain of the auxin-binding protein ABP1, induce stomatal opening and decrease guard-cell pHi, as monitored with the acetomethoxy ester of the ratiometric pH indicator Snarf-1. Similar activity was shown by a monoclonal antibody against the same domain. The C-terminal dodecapeptide, Pz152-163 of maize ABP1 (ABPzm1) induced guard-cell alkalinization and closed stomata, as did Fab fragments of a monoclonal antibody (MAC 256) recognising the C-terminal region of ABPzm1. By implicating, for the first time, an auxin-binding protein in mediation of an auxin-dependent physiological response, these findings strongly support an auxin-receptor role for ABP1. |
| doi_str_mv | 10.1007/s004250050359 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_80043418</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>23385291</jstor_id><sourcerecordid>23385291</sourcerecordid><originalsourceid>FETCH-LOGICAL-c363t-d7901b56caac77a8e8789f187ff6d6e7c55569ccb71a9b76983f99cde60c0f0b3</originalsourceid><addsrcrecordid>eNpVkE1PFTEUhhujwQu4dGmYhWE3ctpOv5aEqJiQuFDWkzOdlpTMdIa2k8i_p3pvEFY9zfP0zelLyEcKXyiAusgAHRMAArgwb8iOdpy1DDr9luwA6gyGi_fkOOd7gAqVOiJHRuqOS7YjeLn9CbEdQhxDvGvXtBQXYoOxhGEZg8t1HJvVrSWM9RKinzYXrWtyWWYsODXL6mJ9-s_DqbjU2MeyrBPmOdhmvT4l7zxO2X04nCfk9tvX31fX7c3P7z-uLm9ayyUv7agM0EFIi2iVQu200sZTrbyXo3TKCiGksXZQFM2gpNHcG2NHJ8GCh4GfkPN9bv3Dw-Zy6eeQrZsmjG7Zcq9rT7yjuortXrRpyTk5368pzJgeewr930r7V5VW_9MheBtmNz7bhw4r_3zgmC1OPmG0IT9rjHe87v4_5r5Wl15grgUztPKzPfe49HiXasTtLwaUA9OGMVD8CTVmkdk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80043418</pqid></control><display><type>article</type><title>Auxin-binding-protein antibodies and peptides influence stomatal opening and alter cytoplasmic pH</title><source>MEDLINE</source><source>SpringerLink</source><source>JSTOR</source><creator>Gehring, C.A ; McConchie, R.M ; Venis, M.A ; Parish, R.W</creator><creatorcontrib>Gehring, C.A ; McConchie, R.M ; Venis, M.A ; Parish, R.W</creatorcontrib><description>Previous work has shown that stomatal opening induced by indole-3-acetic acid (IAA) in epidermal strips of the orchid Paphiopedilum tonsum L. is preceded by a reduction in cytoplasmic pH (pHi) of the guard cells. We now report that Fab fragments of an auxin-agonist antibody (D16), directed against a putative auxin-binding domain of the auxin-binding protein ABP1, induce stomatal opening and decrease guard-cell pHi, as monitored with the acetomethoxy ester of the ratiometric pH indicator Snarf-1. Similar activity was shown by a monoclonal antibody against the same domain. The C-terminal dodecapeptide, Pz152-163 of maize ABP1 (ABPzm1) induced guard-cell alkalinization and closed stomata, as did Fab fragments of a monoclonal antibody (MAC 256) recognising the C-terminal region of ABPzm1. By implicating, for the first time, an auxin-binding protein in mediation of an auxin-dependent physiological response, these findings strongly support an auxin-receptor role for ABP1.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s004250050359</identifier><identifier>PMID: 9684362</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>Agronomy. Soil science and plant productions ; Animals ; Antibodies ; Antibodies - immunology ; Antibodies, Monoclonal - immunology ; Auxins ; Binding Sites ; Biological and medical sciences ; Cell membranes ; Corn ; Cytoplasm ; Economic plant physiology ; epidermis ; Fundamental and applied biological sciences. Psychology ; Guard cells ; Hydrogen-Ion Concentration ; immunochemistry ; indole acetic acid ; Indoleacetic Acids - metabolism ; Mice ; Monoclonal antibodies ; Paphiopedilum ; Peptides - metabolism ; Plant Growth Regulators ; Plant physiology and development ; plant proteins ; Plant Proteins - immunology ; Plant Proteins - metabolism ; Proteins ; Protoplasts ; Receptors, Cell Surface - immunology ; Receptors, Cell Surface - metabolism ; Space life sciences ; Stomata ; stomatal movement ; Water and solutes. Absorption, translocation and permeability ; Water relations, transpiration, stomata</subject><ispartof>Planta, 1998-08, Vol.205 (4), p.581-586</ispartof><rights>Springer-Verlag Berlin Heidelberg 1998</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-d7901b56caac77a8e8789f187ff6d6e7c55569ccb71a9b76983f99cde60c0f0b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23385291$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23385291$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2343555$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9684362$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gehring, C.A</creatorcontrib><creatorcontrib>McConchie, R.M</creatorcontrib><creatorcontrib>Venis, M.A</creatorcontrib><creatorcontrib>Parish, R.W</creatorcontrib><title>Auxin-binding-protein antibodies and peptides influence stomatal opening and alter cytoplasmic pH</title><title>Planta</title><addtitle>Planta</addtitle><description>Previous work has shown that stomatal opening induced by indole-3-acetic acid (IAA) in epidermal strips of the orchid Paphiopedilum tonsum L. is preceded by a reduction in cytoplasmic pH (pHi) of the guard cells. We now report that Fab fragments of an auxin-agonist antibody (D16), directed against a putative auxin-binding domain of the auxin-binding protein ABP1, induce stomatal opening and decrease guard-cell pHi, as monitored with the acetomethoxy ester of the ratiometric pH indicator Snarf-1. Similar activity was shown by a monoclonal antibody against the same domain. The C-terminal dodecapeptide, Pz152-163 of maize ABP1 (ABPzm1) induced guard-cell alkalinization and closed stomata, as did Fab fragments of a monoclonal antibody (MAC 256) recognising the C-terminal region of ABPzm1. By implicating, for the first time, an auxin-binding protein in mediation of an auxin-dependent physiological response, these findings strongly support an auxin-receptor role for ABP1.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies - immunology</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Auxins</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Cell membranes</subject><subject>Corn</subject><subject>Cytoplasm</subject><subject>Economic plant physiology</subject><subject>epidermis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guard cells</subject><subject>Hydrogen-Ion Concentration</subject><subject>immunochemistry</subject><subject>indole acetic acid</subject><subject>Indoleacetic Acids - metabolism</subject><subject>Mice</subject><subject>Monoclonal antibodies</subject><subject>Paphiopedilum</subject><subject>Peptides - metabolism</subject><subject>Plant Growth Regulators</subject><subject>Plant physiology and development</subject><subject>plant proteins</subject><subject>Plant Proteins - immunology</subject><subject>Plant Proteins - metabolism</subject><subject>Proteins</subject><subject>Protoplasts</subject><subject>Receptors, Cell Surface - immunology</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Space life sciences</subject><subject>Stomata</subject><subject>stomatal movement</subject><subject>Water and solutes. Absorption, translocation and permeability</subject><subject>Water relations, transpiration, stomata</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE1PFTEUhhujwQu4dGmYhWE3ctpOv5aEqJiQuFDWkzOdlpTMdIa2k8i_p3pvEFY9zfP0zelLyEcKXyiAusgAHRMAArgwb8iOdpy1DDr9luwA6gyGi_fkOOd7gAqVOiJHRuqOS7YjeLn9CbEdQhxDvGvXtBQXYoOxhGEZg8t1HJvVrSWM9RKinzYXrWtyWWYsODXL6mJ9-s_DqbjU2MeyrBPmOdhmvT4l7zxO2X04nCfk9tvX31fX7c3P7z-uLm9ayyUv7agM0EFIi2iVQu200sZTrbyXo3TKCiGksXZQFM2gpNHcG2NHJ8GCh4GfkPN9bv3Dw-Zy6eeQrZsmjG7Zcq9rT7yjuortXrRpyTk5368pzJgeewr930r7V5VW_9MheBtmNz7bhw4r_3zgmC1OPmG0IT9rjHe87v4_5r5Wl15grgUztPKzPfe49HiXasTtLwaUA9OGMVD8CTVmkdk</recordid><startdate>19980801</startdate><enddate>19980801</enddate><creator>Gehring, C.A</creator><creator>McConchie, R.M</creator><creator>Venis, M.A</creator><creator>Parish, R.W</creator><general>Springer-Verlag</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980801</creationdate><title>Auxin-binding-protein antibodies and peptides influence stomatal opening and alter cytoplasmic pH</title><author>Gehring, C.A ; McConchie, R.M ; Venis, M.A ; Parish, R.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-d7901b56caac77a8e8789f187ff6d6e7c55569ccb71a9b76983f99cde60c0f0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies - immunology</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Auxins</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Cell membranes</topic><topic>Corn</topic><topic>Cytoplasm</topic><topic>Economic plant physiology</topic><topic>epidermis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guard cells</topic><topic>Hydrogen-Ion Concentration</topic><topic>immunochemistry</topic><topic>indole acetic acid</topic><topic>Indoleacetic Acids - metabolism</topic><topic>Mice</topic><topic>Monoclonal antibodies</topic><topic>Paphiopedilum</topic><topic>Peptides - metabolism</topic><topic>Plant Growth Regulators</topic><topic>Plant physiology and development</topic><topic>plant proteins</topic><topic>Plant Proteins - immunology</topic><topic>Plant Proteins - metabolism</topic><topic>Proteins</topic><topic>Protoplasts</topic><topic>Receptors, Cell Surface - immunology</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Space life sciences</topic><topic>Stomata</topic><topic>stomatal movement</topic><topic>Water and solutes. Absorption, translocation and permeability</topic><topic>Water relations, transpiration, stomata</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gehring, C.A</creatorcontrib><creatorcontrib>McConchie, R.M</creatorcontrib><creatorcontrib>Venis, M.A</creatorcontrib><creatorcontrib>Parish, R.W</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gehring, C.A</au><au>McConchie, R.M</au><au>Venis, M.A</au><au>Parish, R.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Auxin-binding-protein antibodies and peptides influence stomatal opening and alter cytoplasmic pH</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>1998-08-01</date><risdate>1998</risdate><volume>205</volume><issue>4</issue><spage>581</spage><epage>586</epage><pages>581-586</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>Previous work has shown that stomatal opening induced by indole-3-acetic acid (IAA) in epidermal strips of the orchid Paphiopedilum tonsum L. is preceded by a reduction in cytoplasmic pH (pHi) of the guard cells. We now report that Fab fragments of an auxin-agonist antibody (D16), directed against a putative auxin-binding domain of the auxin-binding protein ABP1, induce stomatal opening and decrease guard-cell pHi, as monitored with the acetomethoxy ester of the ratiometric pH indicator Snarf-1. Similar activity was shown by a monoclonal antibody against the same domain. The C-terminal dodecapeptide, Pz152-163 of maize ABP1 (ABPzm1) induced guard-cell alkalinization and closed stomata, as did Fab fragments of a monoclonal antibody (MAC 256) recognising the C-terminal region of ABPzm1. By implicating, for the first time, an auxin-binding protein in mediation of an auxin-dependent physiological response, these findings strongly support an auxin-receptor role for ABP1.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><pmid>9684362</pmid><doi>10.1007/s004250050359</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0935 |
| ispartof | Planta, 1998-08, Vol.205 (4), p.581-586 |
| issn | 0032-0935 1432-2048 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80043418 |
| source | MEDLINE; SpringerLink; JSTOR |
| subjects | Agronomy. Soil science and plant productions Animals Antibodies Antibodies - immunology Antibodies, Monoclonal - immunology Auxins Binding Sites Biological and medical sciences Cell membranes Corn Cytoplasm Economic plant physiology epidermis Fundamental and applied biological sciences. Psychology Guard cells Hydrogen-Ion Concentration immunochemistry indole acetic acid Indoleacetic Acids - metabolism Mice Monoclonal antibodies Paphiopedilum Peptides - metabolism Plant Growth Regulators Plant physiology and development plant proteins Plant Proteins - immunology Plant Proteins - metabolism Proteins Protoplasts Receptors, Cell Surface - immunology Receptors, Cell Surface - metabolism Space life sciences Stomata stomatal movement Water and solutes. Absorption, translocation and permeability Water relations, transpiration, stomata |
| title | Auxin-binding-protein antibodies and peptides influence stomatal opening and alter cytoplasmic pH |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T08%3A23%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Auxin-binding-protein%20antibodies%20and%20peptides%20influence%20stomatal%20opening%20and%20alter%20cytoplasmic%20pH&rft.jtitle=Planta&rft.au=Gehring,%20C.A&rft.date=1998-08-01&rft.volume=205&rft.issue=4&rft.spage=581&rft.epage=586&rft.pages=581-586&rft.issn=0032-0935&rft.eissn=1432-2048&rft.coden=PLANAB&rft_id=info:doi/10.1007/s004250050359&rft_dat=%3Cjstor_proqu%3E23385291%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=80043418&rft_id=info:pmid/9684362&rft_jstor_id=23385291&rfr_iscdi=true |