Dormancy-associated gene expression in pea axillary buds.: Cloning and expression of PsDRM1 and PsDRM2
Pea (Pisum sativum L. cv. Alaska) axillary buds can be stimulated to cycle between dormant and growing states. Dormant buds synthesize unique proteins and are as metabolically active as growing buds. Two cDNAs, PsDRM1 and PsDRM2, were isolated from a dormant bud library. The deduced amino acid seque...
Gespeichert in:
| Veröffentlicht in: | Planta 1998-08, Vol.205 (4), p.547-552 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 552 |
|---|---|
| container_issue | 4 |
| container_start_page | 547 |
| container_title | Planta |
| container_volume | 205 |
| creator | Stafstrom, Joel P. Ripley, Bret D. Devitt, Michelle L. Drake, Beth |
| description | Pea (Pisum sativum L. cv. Alaska) axillary buds can be stimulated to cycle between dormant and growing states. Dormant buds synthesize unique proteins and are as metabolically active as growing buds. Two cDNAs, PsDRM1 and PsDRM2, were isolated from a dormant bud library. The deduced amino acid sequence of PsDRM1 (111 residues) is 75% identical to that of an auxin-repressed strawberry clone. PsDRM2 encodes a putative protein containing 129 residues, which includes 11 repeats of the sequence [G]-GGGY[H][N] (the bracketed residues may be absent). PsDRM2 is related to cold- and ABA-stimulated clones from alfalfa. Decapitating the terminal bud rapidly stimulates dormant axillary buds to begin growing. The abundance of PsDRM1 mRNA in axillary buds declines 20-fold within 6 h of decapitation; it quickly reaccumulates when buds become dormant again. The level of PsDRM2 mRNA is about three fold lower in growing buds than in dormant buds. Expression of PsDRM1 is enhanced in other non-growing organs (roots ≫root apices; fully-elongated stems >elongating stems), and thus is an excellent "dormancy" marker. In contrast, PsDRM2 expression is not dormancy-associated in other organs. |
| doi_str_mv | 10.1007/s004250050354 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_80043121</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>23385286</jstor_id><sourcerecordid>23385286</sourcerecordid><originalsourceid>FETCH-LOGICAL-j172t-1964fbe749512fe79f15115d15c63d496de29da177e949310f490593b6c2066c3</originalsourceid><addsrcrecordid>eNpNkM1Lw0AUxBdRaq0ePQp7EG-pb7-SrDdp_YKKInoOm-ymbEk2MZtA-9-71KA9vYHfMMwbhC4JzAlAcusBOBUAApjgR2hKOKMRBZ4eoylA0CCZOEVn3m8AAkySCZrIOOVMyCkql01XK1fsIuV9U1jVG43Xxhlstm1nvLeNw9bh1iistraqVLfD-aD9_A4vqsZZt8bK6UN3U-J3v_x4JXuwl_QcnZSq8uZivDP09fjwuXiOVm9PL4v7VbQhCe0jImNe5ibhUhBamkSWRBAiNBFFzDSXsTZUakWSxEguGYGSSxCS5XFBIY4LNkM3v7lt13wPxvdZbX1hQm1nmsFnaRiLEUqC8Wo0DnltdNZ2tg6vZeMwgV-PXPlCVWUXNrL-z0YZZ6Hkf8zG9013gFkqaBqzH9QdeR8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80043121</pqid></control><display><type>article</type><title>Dormancy-associated gene expression in pea axillary buds.: Cloning and expression of PsDRM1 and PsDRM2</title><source>Jstor Complete Legacy</source><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Stafstrom, Joel P. ; Ripley, Bret D. ; Devitt, Michelle L. ; Drake, Beth</creator><creatorcontrib>Stafstrom, Joel P. ; Ripley, Bret D. ; Devitt, Michelle L. ; Drake, Beth</creatorcontrib><description>Pea (Pisum sativum L. cv. Alaska) axillary buds can be stimulated to cycle between dormant and growing states. Dormant buds synthesize unique proteins and are as metabolically active as growing buds. Two cDNAs, PsDRM1 and PsDRM2, were isolated from a dormant bud library. The deduced amino acid sequence of PsDRM1 (111 residues) is 75% identical to that of an auxin-repressed strawberry clone. PsDRM2 encodes a putative protein containing 129 residues, which includes 11 repeats of the sequence [G]-GGGY[H][N] (the bracketed residues may be absent). PsDRM2 is related to cold- and ABA-stimulated clones from alfalfa. Decapitating the terminal bud rapidly stimulates dormant axillary buds to begin growing. The abundance of PsDRM1 mRNA in axillary buds declines 20-fold within 6 h of decapitation; it quickly reaccumulates when buds become dormant again. The level of PsDRM2 mRNA is about three fold lower in growing buds than in dormant buds. Expression of PsDRM1 is enhanced in other non-growing organs (roots ≫root apices; fully-elongated stems >elongating stems), and thus is an excellent "dormancy" marker. In contrast, PsDRM2 expression is not dormancy-associated in other organs.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s004250050354</identifier><identifier>PMID: 9684359</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>Agronomy. Soil science and plant productions ; Amino Acid Sequence ; Auxins ; Biological and medical sciences ; Complementary DNA ; Decapitation ; Dormancy ; Economic plant physiology ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation, Plant ; Genes, Plant ; Growth and development ; Messenger RNA ; Molecular Sequence Data ; Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence ; Peas ; Pisum sativum - genetics ; Plant growth ; Plant growth. Development of the storage organs ; Plant physiology and development ; Plant Proteins - genetics ; Plants ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Stems ; Vegetative apparatus, growth and morphogenesis. Senescence</subject><ispartof>Planta, 1998-08, Vol.205 (4), p.547-552</ispartof><rights>Springer-Verlag Berlin Heidelberg 1998</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23385286$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23385286$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2343495$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9684359$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stafstrom, Joel P.</creatorcontrib><creatorcontrib>Ripley, Bret D.</creatorcontrib><creatorcontrib>Devitt, Michelle L.</creatorcontrib><creatorcontrib>Drake, Beth</creatorcontrib><title>Dormancy-associated gene expression in pea axillary buds.: Cloning and expression of PsDRM1 and PsDRM2</title><title>Planta</title><addtitle>Planta</addtitle><description>Pea (Pisum sativum L. cv. Alaska) axillary buds can be stimulated to cycle between dormant and growing states. Dormant buds synthesize unique proteins and are as metabolically active as growing buds. Two cDNAs, PsDRM1 and PsDRM2, were isolated from a dormant bud library. The deduced amino acid sequence of PsDRM1 (111 residues) is 75% identical to that of an auxin-repressed strawberry clone. PsDRM2 encodes a putative protein containing 129 residues, which includes 11 repeats of the sequence [G]-GGGY[H][N] (the bracketed residues may be absent). PsDRM2 is related to cold- and ABA-stimulated clones from alfalfa. Decapitating the terminal bud rapidly stimulates dormant axillary buds to begin growing. The abundance of PsDRM1 mRNA in axillary buds declines 20-fold within 6 h of decapitation; it quickly reaccumulates when buds become dormant again. The level of PsDRM2 mRNA is about three fold lower in growing buds than in dormant buds. Expression of PsDRM1 is enhanced in other non-growing organs (roots ≫root apices; fully-elongated stems >elongating stems), and thus is an excellent "dormancy" marker. In contrast, PsDRM2 expression is not dormancy-associated in other organs.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Amino Acid Sequence</subject><subject>Auxins</subject><subject>Biological and medical sciences</subject><subject>Complementary DNA</subject><subject>Decapitation</subject><subject>Dormancy</subject><subject>Economic plant physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes, Plant</subject><subject>Growth and development</subject><subject>Messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence</subject><subject>Peas</subject><subject>Pisum sativum - genetics</subject><subject>Plant growth</subject><subject>Plant growth. Development of the storage organs</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - genetics</subject><subject>Plants</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Stems</subject><subject>Vegetative apparatus, growth and morphogenesis. Senescence</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkM1Lw0AUxBdRaq0ePQp7EG-pb7-SrDdp_YKKInoOm-ymbEk2MZtA-9-71KA9vYHfMMwbhC4JzAlAcusBOBUAApjgR2hKOKMRBZ4eoylA0CCZOEVn3m8AAkySCZrIOOVMyCkql01XK1fsIuV9U1jVG43Xxhlstm1nvLeNw9bh1iistraqVLfD-aD9_A4vqsZZt8bK6UN3U-J3v_x4JXuwl_QcnZSq8uZivDP09fjwuXiOVm9PL4v7VbQhCe0jImNe5ibhUhBamkSWRBAiNBFFzDSXsTZUakWSxEguGYGSSxCS5XFBIY4LNkM3v7lt13wPxvdZbX1hQm1nmsFnaRiLEUqC8Wo0DnltdNZ2tg6vZeMwgV-PXPlCVWUXNrL-z0YZZ6Hkf8zG9013gFkqaBqzH9QdeR8</recordid><startdate>199808</startdate><enddate>199808</enddate><creator>Stafstrom, Joel P.</creator><creator>Ripley, Bret D.</creator><creator>Devitt, Michelle L.</creator><creator>Drake, Beth</creator><general>Springer-Verlag</general><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199808</creationdate><title>Dormancy-associated gene expression in pea axillary buds.: Cloning and expression of PsDRM1 and PsDRM2</title><author>Stafstrom, Joel P. ; Ripley, Bret D. ; Devitt, Michelle L. ; Drake, Beth</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j172t-1964fbe749512fe79f15115d15c63d496de29da177e949310f490593b6c2066c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Amino Acid Sequence</topic><topic>Auxins</topic><topic>Biological and medical sciences</topic><topic>Complementary DNA</topic><topic>Decapitation</topic><topic>Dormancy</topic><topic>Economic plant physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes, Plant</topic><topic>Growth and development</topic><topic>Messenger RNA</topic><topic>Molecular Sequence Data</topic><topic>Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence</topic><topic>Peas</topic><topic>Pisum sativum - genetics</topic><topic>Plant growth</topic><topic>Plant growth. Development of the storage organs</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - genetics</topic><topic>Plants</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Stems</topic><topic>Vegetative apparatus, growth and morphogenesis. Senescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stafstrom, Joel P.</creatorcontrib><creatorcontrib>Ripley, Bret D.</creatorcontrib><creatorcontrib>Devitt, Michelle L.</creatorcontrib><creatorcontrib>Drake, Beth</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stafstrom, Joel P.</au><au>Ripley, Bret D.</au><au>Devitt, Michelle L.</au><au>Drake, Beth</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dormancy-associated gene expression in pea axillary buds.: Cloning and expression of PsDRM1 and PsDRM2</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>1998-08</date><risdate>1998</risdate><volume>205</volume><issue>4</issue><spage>547</spage><epage>552</epage><pages>547-552</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>Pea (Pisum sativum L. cv. Alaska) axillary buds can be stimulated to cycle between dormant and growing states. Dormant buds synthesize unique proteins and are as metabolically active as growing buds. Two cDNAs, PsDRM1 and PsDRM2, were isolated from a dormant bud library. The deduced amino acid sequence of PsDRM1 (111 residues) is 75% identical to that of an auxin-repressed strawberry clone. PsDRM2 encodes a putative protein containing 129 residues, which includes 11 repeats of the sequence [G]-GGGY[H][N] (the bracketed residues may be absent). PsDRM2 is related to cold- and ABA-stimulated clones from alfalfa. Decapitating the terminal bud rapidly stimulates dormant axillary buds to begin growing. The abundance of PsDRM1 mRNA in axillary buds declines 20-fold within 6 h of decapitation; it quickly reaccumulates when buds become dormant again. The level of PsDRM2 mRNA is about three fold lower in growing buds than in dormant buds. Expression of PsDRM1 is enhanced in other non-growing organs (roots ≫root apices; fully-elongated stems >elongating stems), and thus is an excellent "dormancy" marker. In contrast, PsDRM2 expression is not dormancy-associated in other organs.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><pmid>9684359</pmid><doi>10.1007/s004250050354</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0935 |
| ispartof | Planta, 1998-08, Vol.205 (4), p.547-552 |
| issn | 0032-0935 1432-2048 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80043121 |
| source | Jstor Complete Legacy; MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Agronomy. Soil science and plant productions Amino Acid Sequence Auxins Biological and medical sciences Complementary DNA Decapitation Dormancy Economic plant physiology Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Plant Genes, Plant Growth and development Messenger RNA Molecular Sequence Data Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence Peas Pisum sativum - genetics Plant growth Plant growth. Development of the storage organs Plant physiology and development Plant Proteins - genetics Plants Sequence Analysis, DNA Sequence Homology, Amino Acid Stems Vegetative apparatus, growth and morphogenesis. Senescence |
| title | Dormancy-associated gene expression in pea axillary buds.: Cloning and expression of PsDRM1 and PsDRM2 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T19%3A36%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dormancy-associated%20gene%20expression%20in%20pea%20axillary%20buds.:%20Cloning%20and%20expression%20of%20PsDRM1%20and%20PsDRM2&rft.jtitle=Planta&rft.au=Stafstrom,%20Joel%20P.&rft.date=1998-08&rft.volume=205&rft.issue=4&rft.spage=547&rft.epage=552&rft.pages=547-552&rft.issn=0032-0935&rft.eissn=1432-2048&rft.coden=PLANAB&rft_id=info:doi/10.1007/s004250050354&rft_dat=%3Cjstor_proqu%3E23385286%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=80043121&rft_id=info:pmid/9684359&rft_jstor_id=23385286&rfr_iscdi=true |