High-level expression of bovine β-lactoglobulin gene in transgenic mice

To study the expression of the bovine β-lactoglobulin (BLG) gene we isolated the BLG gene from a genomic library and introduced it into murine germline. Bovine BLG gene including 2.8 kbp of 5′ and 1.9 kbp of 3′ flanking region was expressed efficiently and mammary gland-specifically in transgenic mi...

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Veröffentlicht in:	Journal of biotechnology 1998-05, Vol.61 (3), p.191-198
Hauptverfasser:	Hyttinen, Juha-Matti, Korhonen, Veli-Pekka, Hiltunen, Mikko O, Myöhänen, Sanna, Jänne, Juhani
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biotechnology Breast - physiology Cattle Cloning, Molecular DNA DNA Methylation Female Fundamental and applied biological sciences. Psychology Gene Dosage Gene Expression - genetics Genetic engineering Genetic technics Lactoglobulins - genetics Living systems studies Methods. Procedures. Technologies Mice Mice, Transgenic Milk Milk Proteins - secretion Promoter Regions, Genetic - genetics Recombinant Proteins - isolation & purification RNA, Messenger - metabolism Sequence Analysis Tissue Transgene Transgenic animals Transgenic animals and transgenic plants β-Lactoglobulin
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