Validation and testing of a new iontophoretic continuous flow through transport cell
A new continuous flow through transport cell is presented. The design is based on a minimisation of contributions of the cell conformation and experimental protocol to the overall transport kinetics. The system is validated by measuring the washout period of methylene blue and apomorphine. Prelimina...
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| Veröffentlicht in: | Journal of controlled release 1998-01, Vol.51 (1), p.85-91 |
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| creator | van der Geest, R Danhof, M Boddé, H.E |
| description | A new continuous flow through transport cell is presented. The design is based on a minimisation of contributions of the cell conformation and experimental protocol to the overall transport kinetics. The system is validated by measuring the washout period of methylene blue and apomorphine. Preliminary results on the iontophoretic transport of apomorphine across human stratum corneum are presented. These data are used to calculate the deviation of the measured apparent flux from the calculated intrinsic flux across the membrane. The steady state iontophoretic flux was 90±6 nmol
cm
−2
h
−1 when a 15 mM apomorphine solution was applied in the anodal chamber at a current density of 500 μA
cm
2. At any point in time the contribution of the cell to the transport rate was ≤3%. The supporting membrane does not contribute significantly to the overall transport rates. Sink conditions are guaranteed at a flow rate ≥6.5 ml
h
−1. For this new transport cell it can be concluded that the apparent flux that was measured, at validated experimental conditions, is a reliable estimate for the intrinsic flux. |
| doi_str_mv | 10.1016/S0168-3659(97)00156-9 |
| format | Article |
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cm
−2
h
−1 when a 15 mM apomorphine solution was applied in the anodal chamber at a current density of 500 μA
cm
2. At any point in time the contribution of the cell to the transport rate was ≤3%. The supporting membrane does not contribute significantly to the overall transport rates. Sink conditions are guaranteed at a flow rate ≥6.5 ml
h
−1. For this new transport cell it can be concluded that the apparent flux that was measured, at validated experimental conditions, is a reliable estimate for the intrinsic flux.</description><identifier>ISSN: 0168-3659</identifier><identifier>EISSN: 1873-4995</identifier><identifier>DOI: 10.1016/S0168-3659(97)00156-9</identifier><identifier>PMID: 9685906</identifier><identifier>CODEN: JCREEC</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Apomorphine ; Apomorphine - administration & dosage ; Apomorphine - pharmacokinetics ; Biological and medical sciences ; Catecholaminergic system ; Diffusion ; Diffusion cell ; Dopamine Agonists - administration & dosage ; Dopamine Agonists - pharmacokinetics ; General pharmacology ; Humans ; In vitro ; Iontophoresis ; Iontophoresis - instrumentation ; Iontophoresis - methods ; Medical sciences ; Neuropharmacology ; Neurotransmitters. Neurotransmission. Receptors ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Reproducibility of Results ; Skin - metabolism ; Skin Absorption - physiology ; Transport kinetics</subject><ispartof>Journal of controlled release, 1998-01, Vol.51 (1), p.85-91</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-ba1335f413f508cf0f4c835cf2712d47895914fecc8e324f0b0b947b1c73a7b93</citedby><cites>FETCH-LOGICAL-c389t-ba1335f413f508cf0f4c835cf2712d47895914fecc8e324f0b0b947b1c73a7b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0168-3659(97)00156-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2132536$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9685906$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van der Geest, R</creatorcontrib><creatorcontrib>Danhof, M</creatorcontrib><creatorcontrib>Boddé, H.E</creatorcontrib><title>Validation and testing of a new iontophoretic continuous flow through transport cell</title><title>Journal of controlled release</title><addtitle>J Control Release</addtitle><description>A new continuous flow through transport cell is presented. The design is based on a minimisation of contributions of the cell conformation and experimental protocol to the overall transport kinetics. The system is validated by measuring the washout period of methylene blue and apomorphine. Preliminary results on the iontophoretic transport of apomorphine across human stratum corneum are presented. These data are used to calculate the deviation of the measured apparent flux from the calculated intrinsic flux across the membrane. The steady state iontophoretic flux was 90±6 nmol
cm
−2
h
−1 when a 15 mM apomorphine solution was applied in the anodal chamber at a current density of 500 μA
cm
2. At any point in time the contribution of the cell to the transport rate was ≤3%. The supporting membrane does not contribute significantly to the overall transport rates. Sink conditions are guaranteed at a flow rate ≥6.5 ml
h
−1. For this new transport cell it can be concluded that the apparent flux that was measured, at validated experimental conditions, is a reliable estimate for the intrinsic flux.</description><subject>Apomorphine</subject><subject>Apomorphine - administration & dosage</subject><subject>Apomorphine - pharmacokinetics</subject><subject>Biological and medical sciences</subject><subject>Catecholaminergic system</subject><subject>Diffusion</subject><subject>Diffusion cell</subject><subject>Dopamine Agonists - administration & dosage</subject><subject>Dopamine Agonists - pharmacokinetics</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>In vitro</subject><subject>Iontophoresis</subject><subject>Iontophoresis - instrumentation</subject><subject>Iontophoresis - methods</subject><subject>Medical sciences</subject><subject>Neuropharmacology</subject><subject>Neurotransmitters. Neurotransmission. Receptors</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Reproducibility of Results</subject><subject>Skin - metabolism</subject><subject>Skin Absorption - physiology</subject><subject>Transport kinetics</subject><issn>0168-3659</issn><issn>1873-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1LBCEchiWK2j7-hMBDRB2mdBxHPUUsfUHQoa2rOI62xuy4qVP03-fsLnvt4g95H_29PACcYnSFEa6vX_PBC1JTcSHYJUKY1oXYARPMGSkqIegumGyRA3AY4ydCiJKK7YN9UXMqUD0Bs3fVuVYl53uo-hYmE5PrP6C3UMHe_MAcJL-c-2CS01Dnm-sHP0RoO_8D0zz44WMOU1B9XPqQoDZddwz2rOqiOdnMI_B2fzebPhbPLw9P09vnQhMuUtEoTAi1FSaWIq4tspXmhGpbMly2FeOCClxZozU3pKwsalAjKtZgzYhijSBH4Hz97zL4ryE3lwsXxwKqN7mi5AgRIQjJIF2DOvgYg7FyGdxChV-JkRxtypVNOaqSgsmVTTkuON0sGJqFabevNvpyfrbJVdSqs9mCdnGLlZiUlIzYzRozWca3M0FG7UyvTeuC0Um23v1T5A_WIJH3</recordid><startdate>19980123</startdate><enddate>19980123</enddate><creator>van der Geest, R</creator><creator>Danhof, M</creator><creator>Boddé, H.E</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980123</creationdate><title>Validation and testing of a new iontophoretic continuous flow through transport cell</title><author>van der Geest, R ; Danhof, M ; Boddé, H.E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-ba1335f413f508cf0f4c835cf2712d47895914fecc8e324f0b0b947b1c73a7b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Apomorphine</topic><topic>Apomorphine - administration & dosage</topic><topic>Apomorphine - pharmacokinetics</topic><topic>Biological and medical sciences</topic><topic>Catecholaminergic system</topic><topic>Diffusion</topic><topic>Diffusion cell</topic><topic>Dopamine Agonists - administration & dosage</topic><topic>Dopamine Agonists - pharmacokinetics</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>In vitro</topic><topic>Iontophoresis</topic><topic>Iontophoresis - instrumentation</topic><topic>Iontophoresis - methods</topic><topic>Medical sciences</topic><topic>Neuropharmacology</topic><topic>Neurotransmitters. Neurotransmission. Receptors</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Reproducibility of Results</topic><topic>Skin - metabolism</topic><topic>Skin Absorption - physiology</topic><topic>Transport kinetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van der Geest, R</creatorcontrib><creatorcontrib>Danhof, M</creatorcontrib><creatorcontrib>Boddé, H.E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of controlled release</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van der Geest, R</au><au>Danhof, M</au><au>Boddé, H.E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Validation and testing of a new iontophoretic continuous flow through transport cell</atitle><jtitle>Journal of controlled release</jtitle><addtitle>J Control Release</addtitle><date>1998-01-23</date><risdate>1998</risdate><volume>51</volume><issue>1</issue><spage>85</spage><epage>91</epage><pages>85-91</pages><issn>0168-3659</issn><eissn>1873-4995</eissn><coden>JCREEC</coden><abstract>A new continuous flow through transport cell is presented. The design is based on a minimisation of contributions of the cell conformation and experimental protocol to the overall transport kinetics. The system is validated by measuring the washout period of methylene blue and apomorphine. Preliminary results on the iontophoretic transport of apomorphine across human stratum corneum are presented. These data are used to calculate the deviation of the measured apparent flux from the calculated intrinsic flux across the membrane. The steady state iontophoretic flux was 90±6 nmol
cm
−2
h
−1 when a 15 mM apomorphine solution was applied in the anodal chamber at a current density of 500 μA
cm
2. At any point in time the contribution of the cell to the transport rate was ≤3%. The supporting membrane does not contribute significantly to the overall transport rates. Sink conditions are guaranteed at a flow rate ≥6.5 ml
h
−1. For this new transport cell it can be concluded that the apparent flux that was measured, at validated experimental conditions, is a reliable estimate for the intrinsic flux.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>9685906</pmid><doi>10.1016/S0168-3659(97)00156-9</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of controlled release, 1998-01, Vol.51 (1), p.85-91 |
| issn | 0168-3659 1873-4995 |
| language | eng |
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| subjects | Apomorphine Apomorphine - administration & dosage Apomorphine - pharmacokinetics Biological and medical sciences Catecholaminergic system Diffusion Diffusion cell Dopamine Agonists - administration & dosage Dopamine Agonists - pharmacokinetics General pharmacology Humans In vitro Iontophoresis Iontophoresis - instrumentation Iontophoresis - methods Medical sciences Neuropharmacology Neurotransmitters. Neurotransmission. Receptors Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Reproducibility of Results Skin - metabolism Skin Absorption - physiology Transport kinetics |
| title | Validation and testing of a new iontophoretic continuous flow through transport cell |
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