Detection and quantitation of cellularly derived amyloid β peptides by immunoprecipitation-HPLC-MS
A quantitative method for detection of amyloid β peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described. Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of Aβ 1–40 and Aβ 1–42 derived from fetal guinea pig cell media and cell lysates....
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| Veröffentlicht in: | FEBS letters 1998-07, Vol.430 (3), p.419-423 |
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| container_title | FEBS letters |
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| creator | Clarke, Nigel J Tomlinson, Andy J Ohyagi, Yasumasa Younkin, Steven Naylor, Stephen |
| description | A quantitative method for detection of amyloid β peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described. Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of Aβ 1–40 and Aβ 1–42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for Aβ 1–40 and Aβ 1–42 peptides present in Alzheimer's disease (AD), but allows detection of other Aβ peptide species that may also play a role in the onset of AD in humans. |
| doi_str_mv | 10.1016/S0014-5793(98)00706-6 |
| format | Article |
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Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of Aβ 1–40 and Aβ 1–42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for Aβ 1–40 and Aβ 1–42 peptides present in Alzheimer's disease (AD), but allows detection of other Aβ peptide species that may also play a role in the onset of AD in humans.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(98)00706-6</identifier><identifier>PMID: 9688584</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>AcOH, acetic acid ; AD, Alzheimer's disease ; Alzheimer's disease ; Amyloid beta-Peptides - analysis ; Amyloid β ; Animals ; APP, amyloid precursor protein ; Aβ 1–40, amyloid β peptide consisting of APP residues 672–711 ; Aβ 1–42, amyloid β peptide consisting of APP residues 672–713 ; Aβ, amyloid beta peptide ; Brain Chemistry ; Cell Extracts ; CH3CN, acetonitrile ; Chromatography, High Pressure Liquid - methods ; Culture Media, Conditioned ; ELISA, enzyme linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; HPLC ; HPLC, high performance liquid chromatography ; Humans ; Immunoprecipitation ; IP, immunoprecipitation ; LC, liquid chromatography ; Mass spectrometry ; Mass Spectrometry - methods ; MS, mass spectrometry ; n-PrOH, n-propanol ; PATRIC, position and time resolved ion counter ; PBS, phosphate buffered saline ; Peptides - analysis ; Precipitin Tests - methods ; RIPA buffer, 150 mM sodium chloride, 1% nonidet, 0.5% deoxycholate acid, 0.1% SDS, 50 mM Tris-base ; SDS, sodium dodecyl sulfate ; sELISA, sandwich ELISA ; Sensitivity and Specificity ; SIP, single ion profile ; Swine ; TFA, trifluoroacetic acid</subject><ispartof>FEBS letters, 1998-07, Vol.430 (3), p.419-423</ispartof><rights>1998 Federation of European Biochemical Societies</rights><rights>FEBS Letters 430 (1998) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4256-f0ff18cad0c738cdb941d58b2d12ed19312cb8598e0d56d46fc5e1b1d5c9c4c23</citedby><cites>FETCH-LOGICAL-c4256-f0ff18cad0c738cdb941d58b2d12ed19312cb8598e0d56d46fc5e1b1d5c9c4c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2898%2900706-6$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-5793(98)00706-6$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1416,1432,3548,27923,27924,45573,45574,45994,46408,46832</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9688584$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clarke, Nigel J</creatorcontrib><creatorcontrib>Tomlinson, Andy J</creatorcontrib><creatorcontrib>Ohyagi, Yasumasa</creatorcontrib><creatorcontrib>Younkin, Steven</creatorcontrib><creatorcontrib>Naylor, Stephen</creatorcontrib><title>Detection and quantitation of cellularly derived amyloid β peptides by immunoprecipitation-HPLC-MS</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>A quantitative method for detection of amyloid β peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described. Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of Aβ 1–40 and Aβ 1–42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for Aβ 1–40 and Aβ 1–42 peptides present in Alzheimer's disease (AD), but allows detection of other Aβ peptide species that may also play a role in the onset of AD in humans.</description><subject>AcOH, acetic acid</subject><subject>AD, Alzheimer's disease</subject><subject>Alzheimer's disease</subject><subject>Amyloid beta-Peptides - analysis</subject><subject>Amyloid β</subject><subject>Animals</subject><subject>APP, amyloid precursor protein</subject><subject>Aβ 1–40, amyloid β peptide consisting of APP residues 672–711</subject><subject>Aβ 1–42, amyloid β peptide consisting of APP residues 672–713</subject><subject>Aβ, amyloid beta peptide</subject><subject>Brain Chemistry</subject><subject>Cell Extracts</subject><subject>CH3CN, acetonitrile</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Culture Media, Conditioned</subject><subject>ELISA, enzyme linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>HPLC</subject><subject>HPLC, high performance liquid chromatography</subject><subject>Humans</subject><subject>Immunoprecipitation</subject><subject>IP, immunoprecipitation</subject><subject>LC, liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass Spectrometry - methods</subject><subject>MS, mass spectrometry</subject><subject>n-PrOH, n-propanol</subject><subject>PATRIC, position and time resolved ion counter</subject><subject>PBS, phosphate buffered saline</subject><subject>Peptides - analysis</subject><subject>Precipitin Tests - methods</subject><subject>RIPA buffer, 150 mM sodium chloride, 1% nonidet, 0.5% deoxycholate acid, 0.1% SDS, 50 mM Tris-base</subject><subject>SDS, sodium dodecyl sulfate</subject><subject>sELISA, sandwich ELISA</subject><subject>Sensitivity and Specificity</subject><subject>SIP, single ion profile</subject><subject>Swine</subject><subject>TFA, trifluoroacetic acid</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1O5DAQhS3ECJqfIyB5hZhFGDuJHXuFoAdopB4NEjNry7ErklH-sJNGuRYH4Uwk6RZbWJWq3qtX9ofQGSWXlFD-64kQmkYsk8mFFD8JyQiP-B5aUJElUZJysY8Wn5ZDdBTCMxl7QeUBOpBcCCbSBTK_oQPTuabGurb4pdd15zo9D5oCGyjLvtS-HLAF7zZgsa6GsnEWv7_hFtrOWQg4H7Crqr5uWg_GtbuAaPW4XkZ_nk7Qj0KXAU539Rj9v7v9t1xF67_3D8vrdWTSmPGoIEVBhdGWmCwRxuYypZaJPLY0BktlQmOTCyYFEMu4TXlhGNB89BhpUhMnx-h8m9v65qWH0KnKhekHuoamD0oQkkjOstHItkbjmxA8FKr1rtJ-UJSoCa6a4aqJnJJCzXAVH_fOdgf6vAL7ubWjOeqrrf7qShi-F6rubm_iWZkEKebxdOpqGwUjsI0Dr4JxUBuwbkTcKdu4Lx77ARPFn74</recordid><startdate>19980703</startdate><enddate>19980703</enddate><creator>Clarke, Nigel J</creator><creator>Tomlinson, Andy J</creator><creator>Ohyagi, Yasumasa</creator><creator>Younkin, Steven</creator><creator>Naylor, Stephen</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980703</creationdate><title>Detection and quantitation of cellularly derived amyloid β peptides by immunoprecipitation-HPLC-MS</title><author>Clarke, Nigel J ; Tomlinson, Andy J ; Ohyagi, Yasumasa ; Younkin, Steven ; Naylor, Stephen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4256-f0ff18cad0c738cdb941d58b2d12ed19312cb8598e0d56d46fc5e1b1d5c9c4c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>AcOH, acetic acid</topic><topic>AD, Alzheimer's disease</topic><topic>Alzheimer's disease</topic><topic>Amyloid beta-Peptides - analysis</topic><topic>Amyloid β</topic><topic>Animals</topic><topic>APP, amyloid precursor protein</topic><topic>Aβ 1–40, amyloid β peptide consisting of APP residues 672–711</topic><topic>Aβ 1–42, amyloid β peptide consisting of APP residues 672–713</topic><topic>Aβ, amyloid beta peptide</topic><topic>Brain Chemistry</topic><topic>Cell Extracts</topic><topic>CH3CN, acetonitrile</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Culture Media, Conditioned</topic><topic>ELISA, enzyme linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>HPLC</topic><topic>HPLC, high performance liquid chromatography</topic><topic>Humans</topic><topic>Immunoprecipitation</topic><topic>IP, immunoprecipitation</topic><topic>LC, liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Mass Spectrometry - methods</topic><topic>MS, mass spectrometry</topic><topic>n-PrOH, n-propanol</topic><topic>PATRIC, position and time resolved ion counter</topic><topic>PBS, phosphate buffered saline</topic><topic>Peptides - analysis</topic><topic>Precipitin Tests - methods</topic><topic>RIPA buffer, 150 mM sodium chloride, 1% nonidet, 0.5% deoxycholate acid, 0.1% SDS, 50 mM Tris-base</topic><topic>SDS, sodium dodecyl sulfate</topic><topic>sELISA, sandwich ELISA</topic><topic>Sensitivity and Specificity</topic><topic>SIP, single ion profile</topic><topic>Swine</topic><topic>TFA, trifluoroacetic acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clarke, Nigel J</creatorcontrib><creatorcontrib>Tomlinson, Andy J</creatorcontrib><creatorcontrib>Ohyagi, Yasumasa</creatorcontrib><creatorcontrib>Younkin, Steven</creatorcontrib><creatorcontrib>Naylor, Stephen</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clarke, Nigel J</au><au>Tomlinson, Andy J</au><au>Ohyagi, Yasumasa</au><au>Younkin, Steven</au><au>Naylor, Stephen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection and quantitation of cellularly derived amyloid β peptides by immunoprecipitation-HPLC-MS</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1998-07-03</date><risdate>1998</risdate><volume>430</volume><issue>3</issue><spage>419</spage><epage>423</epage><pages>419-423</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>A quantitative method for detection of amyloid β peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described. Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of Aβ 1–40 and Aβ 1–42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for Aβ 1–40 and Aβ 1–42 peptides present in Alzheimer's disease (AD), but allows detection of other Aβ peptide species that may also play a role in the onset of AD in humans.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>9688584</pmid><doi>10.1016/S0014-5793(98)00706-6</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1998-07, Vol.430 (3), p.419-423 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Wiley Free Content; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | AcOH, acetic acid AD, Alzheimer's disease Alzheimer's disease Amyloid beta-Peptides - analysis Amyloid β Animals APP, amyloid precursor protein Aβ 1–40, amyloid β peptide consisting of APP residues 672–711 Aβ 1–42, amyloid β peptide consisting of APP residues 672–713 Aβ, amyloid beta peptide Brain Chemistry Cell Extracts CH3CN, acetonitrile Chromatography, High Pressure Liquid - methods Culture Media, Conditioned ELISA, enzyme linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods HPLC HPLC, high performance liquid chromatography Humans Immunoprecipitation IP, immunoprecipitation LC, liquid chromatography Mass spectrometry Mass Spectrometry - methods MS, mass spectrometry n-PrOH, n-propanol PATRIC, position and time resolved ion counter PBS, phosphate buffered saline Peptides - analysis Precipitin Tests - methods RIPA buffer, 150 mM sodium chloride, 1% nonidet, 0.5% deoxycholate acid, 0.1% SDS, 50 mM Tris-base SDS, sodium dodecyl sulfate sELISA, sandwich ELISA Sensitivity and Specificity SIP, single ion profile Swine TFA, trifluoroacetic acid |
| title | Detection and quantitation of cellularly derived amyloid β peptides by immunoprecipitation-HPLC-MS |
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