An alternative strategy for the radioimmunoassay of angiotensin peptides using amino-terminal-directed antisera: measurement of eight angiotensin peptides in human plasma
We describe here a method of measuring angiotensin peptides and their carboxy-truncated metabolites in human plasma using N-terminal-directed antisera. Antisera raised against N-acetylated angiotensin (Ang) II and N-acetylated Ang III analogues were used to develop two radioimmunoassays. Extracted p...
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| Veröffentlicht in: | Journal of hypertension 1990-08, Vol.8 (8), p.715-724 |
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| container_title | Journal of hypertension |
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| creator | Lawrence, Anne C Evin, Genevieve Kladis, Athena Campbell, Duncan J |
| description | We describe here a method of measuring angiotensin peptides and their carboxy-truncated metabolites in human plasma using N-terminal-directed antisera. Antisera raised against N-acetylated angiotensin (Ang) II and N-acetylated Ang III analogues were used to develop two radioimmunoassays. Extracted plasma samples were acetylated prior to separation of cross-reacting angiotensin peptides by high-performance liquid chromatography (HPLC). Fractions were assayed with both antisera to obtain measurements for eight angiotensin peptides. Angiotensin levels measured in normal males were (fmol/ml plasma, means ± s.e.m., n=14)Ang-(1—7) 1.0 ± 0.2, Ang II 13.9 ±2.0, Ang-(1-9) 7lt;0.4, Ang I 19.5 ± 2.4, Ang-(2-7) |
| doi_str_mv | 10.1097/00004872-199008000-00005 |
| format | Article |
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Antisera raised against N-acetylated angiotensin (Ang) II and N-acetylated Ang III analogues were used to develop two radioimmunoassays. Extracted plasma samples were acetylated prior to separation of cross-reacting angiotensin peptides by high-performance liquid chromatography (HPLC). Fractions were assayed with both antisera to obtain measurements for eight angiotensin peptides. Angiotensin levels measured in normal males were (fmol/ml plasma, means ± s.e.m., n=14)Ang-(1—7) 1.0 ± 0.2, Ang II 13.9 ±2.0, Ang-(1-9) 7lt;0.4, Ang I 19.5 ± 2.4, Ang-(2-7) <1.1, Ang III 2.9 ± 1.0, Ang-(2-9) <2.1, Ang-(2-10) 2.4 ± 0.8. Hypertensive patients receiving angiotensin converting enzyme (ACE) inhibitor therapy (n=8) had an increase in Angt to 187.3 ± 107.2fmol/ml (P=0.002), and a reduction in Angll to 4.8 ± 1.2fmol/ml (P<0.001). Furthermore, these patients showed a ninefold increase in Ang-(1—7) to 9.7 ± 4.3 fmol/ml (P<0.001), indicating a role for prolylendopeptidase in the metabolism of Ang I in vivo. These N-terminal assays have demonstrated that carboxy-truncated metabolites of Ang I and Angll make little contribution to plasma angiotensin peptides, except during ACE inhibitor therapy. Furthermore, these antisera allow the measurement of Ang I and Angll in the same radioimmunoassay of fractions from HPLC, providing a highly reliable estimate of the AngllAng I ratio.</description><identifier>ISSN: 0263-6352</identifier><identifier>EISSN: 1473-5598</identifier><identifier>DOI: 10.1097/00004872-199008000-00005</identifier><identifier>PMID: 2170511</identifier><language>eng</language><publisher>England: Lippincott-Raven Publishers</publisher><subject>Angiotensin-Converting Enzyme Inhibitors - therapeutic use ; Angiotensins - blood ; Angiotensins - immunology ; Chromatography, High Pressure Liquid ; Humans ; Hypertension - blood ; Hypertension - drug therapy ; Immune Sera ; Male ; Peptide Fragments - blood ; Peptide Fragments - immunology ; Radioimmunoassay - methods</subject><ispartof>Journal of hypertension, 1990-08, Vol.8 (8), p.715-724</ispartof><rights>Lippincott-Raven Publishers.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3365-306a6aa4a3a1b42fb1844bf39c3405eeaa436f8db19b1c73ceb85ed9d6482b433</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2170511$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lawrence, Anne C</creatorcontrib><creatorcontrib>Evin, Genevieve</creatorcontrib><creatorcontrib>Kladis, Athena</creatorcontrib><creatorcontrib>Campbell, Duncan J</creatorcontrib><title>An alternative strategy for the radioimmunoassay of angiotensin peptides using amino-terminal-directed antisera: measurement of eight angiotensin peptides in human plasma</title><title>Journal of hypertension</title><addtitle>J Hypertens</addtitle><description>We describe here a method of measuring angiotensin peptides and their carboxy-truncated metabolites in human plasma using N-terminal-directed antisera. Antisera raised against N-acetylated angiotensin (Ang) II and N-acetylated Ang III analogues were used to develop two radioimmunoassays. Extracted plasma samples were acetylated prior to separation of cross-reacting angiotensin peptides by high-performance liquid chromatography (HPLC). Fractions were assayed with both antisera to obtain measurements for eight angiotensin peptides. Angiotensin levels measured in normal males were (fmol/ml plasma, means ± s.e.m., n=14)Ang-(1—7) 1.0 ± 0.2, Ang II 13.9 ±2.0, Ang-(1-9) 7lt;0.4, Ang I 19.5 ± 2.4, Ang-(2-7) <1.1, Ang III 2.9 ± 1.0, Ang-(2-9) <2.1, Ang-(2-10) 2.4 ± 0.8. Hypertensive patients receiving angiotensin converting enzyme (ACE) inhibitor therapy (n=8) had an increase in Angt to 187.3 ± 107.2fmol/ml (P=0.002), and a reduction in Angll to 4.8 ± 1.2fmol/ml (P<0.001). Furthermore, these patients showed a ninefold increase in Ang-(1—7) to 9.7 ± 4.3 fmol/ml (P<0.001), indicating a role for prolylendopeptidase in the metabolism of Ang I in vivo. These N-terminal assays have demonstrated that carboxy-truncated metabolites of Ang I and Angll make little contribution to plasma angiotensin peptides, except during ACE inhibitor therapy. Furthermore, these antisera allow the measurement of Ang I and Angll in the same radioimmunoassay of fractions from HPLC, providing a highly reliable estimate of the AngllAng I ratio.</description><subject>Angiotensin-Converting Enzyme Inhibitors - therapeutic use</subject><subject>Angiotensins - blood</subject><subject>Angiotensins - immunology</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Humans</subject><subject>Hypertension - blood</subject><subject>Hypertension - drug therapy</subject><subject>Immune Sera</subject><subject>Male</subject><subject>Peptide Fragments - blood</subject><subject>Peptide Fragments - immunology</subject><subject>Radioimmunoassay - methods</subject><issn>0263-6352</issn><issn>1473-5598</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1v1DAQhi1EVZa2PwHJJ24GO7YTh1tV8SVV4gLnaJJMNobYXmyHav8SvxKnu_QEvozn431HmocQKvgbwdvmLS9PmaZiom05NyVjW0k_IzuhGsm0bs1zsuNVLVktdfWCvEzpe5kwbSMvyWUlGq6F2JHft57CkjF6yPYX0pQjZNwf6RQizTPSCKMN1rnVB0gJjjRMFPzehow-WU8PeMh2xETXku0pOOsDK34lwsJGG3HIOBZJtgkjvKMOIa0RHfq8eaHdz_nfjuU_rw5KZYHk4JpcTLAkvDnHK_Ltw_uvd5_Y_ZePn-9u79kgZa2Z5DXUAAokiF5VUy-MUv0k20EqrhFLS9aTGXvR9mJo5IC90Ti2Y61M1Sspr8jrk-8hhp8rptw5mwZcFvAY1tSVa8tye10GzWlwiCGliFN3iNZBPHaCdxum7i-m7gnTY2mTvjrvWHuH45PwzKX01an_EDY46ceyPmDsZiys5u5_9OUf6UKiog</recordid><startdate>199008</startdate><enddate>199008</enddate><creator>Lawrence, Anne C</creator><creator>Evin, Genevieve</creator><creator>Kladis, Athena</creator><creator>Campbell, Duncan J</creator><general>Lippincott-Raven Publishers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199008</creationdate><title>An alternative strategy for the radioimmunoassay of angiotensin peptides using amino-terminal-directed antisera: measurement of eight angiotensin peptides in human plasma</title><author>Lawrence, Anne C ; Evin, Genevieve ; Kladis, Athena ; Campbell, Duncan J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3365-306a6aa4a3a1b42fb1844bf39c3405eeaa436f8db19b1c73ceb85ed9d6482b433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Angiotensin-Converting Enzyme Inhibitors - therapeutic use</topic><topic>Angiotensins - blood</topic><topic>Angiotensins - immunology</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Humans</topic><topic>Hypertension - blood</topic><topic>Hypertension - drug therapy</topic><topic>Immune Sera</topic><topic>Male</topic><topic>Peptide Fragments - blood</topic><topic>Peptide Fragments - immunology</topic><topic>Radioimmunoassay - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lawrence, Anne C</creatorcontrib><creatorcontrib>Evin, Genevieve</creatorcontrib><creatorcontrib>Kladis, Athena</creatorcontrib><creatorcontrib>Campbell, Duncan J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of hypertension</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lawrence, Anne C</au><au>Evin, Genevieve</au><au>Kladis, Athena</au><au>Campbell, Duncan J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An alternative strategy for the radioimmunoassay of angiotensin peptides using amino-terminal-directed antisera: measurement of eight angiotensin peptides in human plasma</atitle><jtitle>Journal of hypertension</jtitle><addtitle>J Hypertens</addtitle><date>1990-08</date><risdate>1990</risdate><volume>8</volume><issue>8</issue><spage>715</spage><epage>724</epage><pages>715-724</pages><issn>0263-6352</issn><eissn>1473-5598</eissn><abstract>We describe here a method of measuring angiotensin peptides and their carboxy-truncated metabolites in human plasma using N-terminal-directed antisera. Antisera raised against N-acetylated angiotensin (Ang) II and N-acetylated Ang III analogues were used to develop two radioimmunoassays. Extracted plasma samples were acetylated prior to separation of cross-reacting angiotensin peptides by high-performance liquid chromatography (HPLC). Fractions were assayed with both antisera to obtain measurements for eight angiotensin peptides. Angiotensin levels measured in normal males were (fmol/ml plasma, means ± s.e.m., n=14)Ang-(1—7) 1.0 ± 0.2, Ang II 13.9 ±2.0, Ang-(1-9) 7lt;0.4, Ang I 19.5 ± 2.4, Ang-(2-7) <1.1, Ang III 2.9 ± 1.0, Ang-(2-9) <2.1, Ang-(2-10) 2.4 ± 0.8. Hypertensive patients receiving angiotensin converting enzyme (ACE) inhibitor therapy (n=8) had an increase in Angt to 187.3 ± 107.2fmol/ml (P=0.002), and a reduction in Angll to 4.8 ± 1.2fmol/ml (P<0.001). Furthermore, these patients showed a ninefold increase in Ang-(1—7) to 9.7 ± 4.3 fmol/ml (P<0.001), indicating a role for prolylendopeptidase in the metabolism of Ang I in vivo. These N-terminal assays have demonstrated that carboxy-truncated metabolites of Ang I and Angll make little contribution to plasma angiotensin peptides, except during ACE inhibitor therapy. Furthermore, these antisera allow the measurement of Ang I and Angll in the same radioimmunoassay of fractions from HPLC, providing a highly reliable estimate of the AngllAng I ratio.</abstract><cop>England</cop><pub>Lippincott-Raven Publishers</pub><pmid>2170511</pmid><doi>10.1097/00004872-199008000-00005</doi><tpages>10</tpages></addata></record> |
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| subjects | Angiotensin-Converting Enzyme Inhibitors - therapeutic use Angiotensins - blood Angiotensins - immunology Chromatography, High Pressure Liquid Humans Hypertension - blood Hypertension - drug therapy Immune Sera Male Peptide Fragments - blood Peptide Fragments - immunology Radioimmunoassay - methods |
| title | An alternative strategy for the radioimmunoassay of angiotensin peptides using amino-terminal-directed antisera: measurement of eight angiotensin peptides in human plasma |
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