Release of Telomeric DNA from Chromosomes in Immortal Human Cells Lacking Telomerase Activity
Some immortal human cell lines lack telomerase activity. These cell lines were found to contain small dispersed DNA hybridizing to TTAGGG repeats. Such DNA was located in their cytoplasm and nuclei. Normal human fibroblasts or telomerase-positive cell lines did not contain such DNA. Upon cloning and...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 1998-07, Vol.248 (2), p.223-227 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 227 |
|---|---|
| container_issue | 2 |
| container_start_page | 223 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 248 |
| creator | Ogino, Hideki Nakabayashi, Kazuhiko Suzuki, Mikio Takahashi, Ei-ichi Fujii, Michihiko Suzuki, Toshikazu Ayusawa, Dai |
| description | Some immortal human cell lines lack telomerase activity. These cell lines were found to contain small dispersed DNA hybridizing to TTAGGG repeats. Such DNA was located in their cytoplasm and nuclei. Normal human fibroblasts or telomerase-positive cell lines did not contain such DNA. Upon cloning and sequencing, it was shown to consist of TTAGGG repeats. When electrophoresed on neutral and alkaline agarose gels, it behaved as double-stranded and linear DNA. These results suggest that telomeric DNA is released from chromosomes in association with maintenance of telomeres in telomerase-negative cell lines. |
| doi_str_mv | 10.1006/bbrc.1998.8875 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80031928</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X98988751</els_id><sourcerecordid>16560352</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-b6f8aef3034a4f28f012f8791d3406db4859f1caf039fc692fbacf97709e76af3</originalsourceid><addsrcrecordid>eNqFkMtLxDAQh4Mouj6u3oScvHWd9JEmx2V9wqIgCl4kpOlEo22jSXfB_96WXb2JlxmY-ebH8BFyzGDKAPhZVQUzZVKKqRBlsUUmDCQkKYN8m0xgIJJUsqc9sh_jGwBjOZe7ZFfysmCsnJDne2xQR6Te0gdsfIvBGXp-O6M2-JbOX4fq4zCO1HX0pm196HVDr5et7ugcmybShTbvrnv5OR_DZqZ3K9d_HZIdq5uIR5t-QB4vLx7m18ni7upmPlskJs94n1TcCo02gyzXuU2FBZZaUUpWZznwuspFIS0z2kImreEytZU2VpYlSCy5ttkBOV3nfgT_ucTYq9ZFM3ynO_TLqARAxmQq_gUZLzhkRTqA0zVogo8xoFUfwbU6fCkGahSvRvFqFK9G8cPBySZ5WbVY_-Ib08NerPc4eFg5DCoah53B2gU0vaq9-yv6G76Hkjk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16560352</pqid></control><display><type>article</type><title>Release of Telomeric DNA from Chromosomes in Immortal Human Cells Lacking Telomerase Activity</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Ogino, Hideki ; Nakabayashi, Kazuhiko ; Suzuki, Mikio ; Takahashi, Ei-ichi ; Fujii, Michihiko ; Suzuki, Toshikazu ; Ayusawa, Dai</creator><creatorcontrib>Ogino, Hideki ; Nakabayashi, Kazuhiko ; Suzuki, Mikio ; Takahashi, Ei-ichi ; Fujii, Michihiko ; Suzuki, Toshikazu ; Ayusawa, Dai</creatorcontrib><description>Some immortal human cell lines lack telomerase activity. These cell lines were found to contain small dispersed DNA hybridizing to TTAGGG repeats. Such DNA was located in their cytoplasm and nuclei. Normal human fibroblasts or telomerase-positive cell lines did not contain such DNA. Upon cloning and sequencing, it was shown to consist of TTAGGG repeats. When electrophoresed on neutral and alkaline agarose gels, it behaved as double-stranded and linear DNA. These results suggest that telomeric DNA is released from chromosomes in association with maintenance of telomeres in telomerase-negative cell lines.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1998.8875</identifier><identifier>PMID: 9675117</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Cell Line ; Chromosomes - genetics ; Cloning, Molecular ; DNA - chemistry ; DNA, Circular - genetics ; Electrophoresis, Agar Gel ; Humans ; Nucleic Acid Conformation ; Nucleic Acid Hybridization ; Repetitive Sequences, Nucleic Acid - genetics ; Sequence Analysis, DNA ; Telomere - genetics</subject><ispartof>Biochemical and biophysical research communications, 1998-07, Vol.248 (2), p.223-227</ispartof><rights>1998 Academic Press</rights><rights>Copyright 1998 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-b6f8aef3034a4f28f012f8791d3406db4859f1caf039fc692fbacf97709e76af3</citedby><cites>FETCH-LOGICAL-c436t-b6f8aef3034a4f28f012f8791d3406db4859f1caf039fc692fbacf97709e76af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X98988751$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9675117$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ogino, Hideki</creatorcontrib><creatorcontrib>Nakabayashi, Kazuhiko</creatorcontrib><creatorcontrib>Suzuki, Mikio</creatorcontrib><creatorcontrib>Takahashi, Ei-ichi</creatorcontrib><creatorcontrib>Fujii, Michihiko</creatorcontrib><creatorcontrib>Suzuki, Toshikazu</creatorcontrib><creatorcontrib>Ayusawa, Dai</creatorcontrib><title>Release of Telomeric DNA from Chromosomes in Immortal Human Cells Lacking Telomerase Activity</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Some immortal human cell lines lack telomerase activity. These cell lines were found to contain small dispersed DNA hybridizing to TTAGGG repeats. Such DNA was located in their cytoplasm and nuclei. Normal human fibroblasts or telomerase-positive cell lines did not contain such DNA. Upon cloning and sequencing, it was shown to consist of TTAGGG repeats. When electrophoresed on neutral and alkaline agarose gels, it behaved as double-stranded and linear DNA. These results suggest that telomeric DNA is released from chromosomes in association with maintenance of telomeres in telomerase-negative cell lines.</description><subject>Cell Line</subject><subject>Chromosomes - genetics</subject><subject>Cloning, Molecular</subject><subject>DNA - chemistry</subject><subject>DNA, Circular - genetics</subject><subject>Electrophoresis, Agar Gel</subject><subject>Humans</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic Acid Hybridization</subject><subject>Repetitive Sequences, Nucleic Acid - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Telomere - genetics</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtLxDAQh4Mouj6u3oScvHWd9JEmx2V9wqIgCl4kpOlEo22jSXfB_96WXb2JlxmY-ebH8BFyzGDKAPhZVQUzZVKKqRBlsUUmDCQkKYN8m0xgIJJUsqc9sh_jGwBjOZe7ZFfysmCsnJDne2xQR6Te0gdsfIvBGXp-O6M2-JbOX4fq4zCO1HX0pm196HVDr5et7ugcmybShTbvrnv5OR_DZqZ3K9d_HZIdq5uIR5t-QB4vLx7m18ni7upmPlskJs94n1TcCo02gyzXuU2FBZZaUUpWZznwuspFIS0z2kImreEytZU2VpYlSCy5ttkBOV3nfgT_ucTYq9ZFM3ynO_TLqARAxmQq_gUZLzhkRTqA0zVogo8xoFUfwbU6fCkGahSvRvFqFK9G8cPBySZ5WbVY_-Ib08NerPc4eFg5DCoah53B2gU0vaq9-yv6G76Hkjk</recordid><startdate>19980720</startdate><enddate>19980720</enddate><creator>Ogino, Hideki</creator><creator>Nakabayashi, Kazuhiko</creator><creator>Suzuki, Mikio</creator><creator>Takahashi, Ei-ichi</creator><creator>Fujii, Michihiko</creator><creator>Suzuki, Toshikazu</creator><creator>Ayusawa, Dai</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19980720</creationdate><title>Release of Telomeric DNA from Chromosomes in Immortal Human Cells Lacking Telomerase Activity</title><author>Ogino, Hideki ; Nakabayashi, Kazuhiko ; Suzuki, Mikio ; Takahashi, Ei-ichi ; Fujii, Michihiko ; Suzuki, Toshikazu ; Ayusawa, Dai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-b6f8aef3034a4f28f012f8791d3406db4859f1caf039fc692fbacf97709e76af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Cell Line</topic><topic>Chromosomes - genetics</topic><topic>Cloning, Molecular</topic><topic>DNA - chemistry</topic><topic>DNA, Circular - genetics</topic><topic>Electrophoresis, Agar Gel</topic><topic>Humans</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleic Acid Hybridization</topic><topic>Repetitive Sequences, Nucleic Acid - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Telomere - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ogino, Hideki</creatorcontrib><creatorcontrib>Nakabayashi, Kazuhiko</creatorcontrib><creatorcontrib>Suzuki, Mikio</creatorcontrib><creatorcontrib>Takahashi, Ei-ichi</creatorcontrib><creatorcontrib>Fujii, Michihiko</creatorcontrib><creatorcontrib>Suzuki, Toshikazu</creatorcontrib><creatorcontrib>Ayusawa, Dai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ogino, Hideki</au><au>Nakabayashi, Kazuhiko</au><au>Suzuki, Mikio</au><au>Takahashi, Ei-ichi</au><au>Fujii, Michihiko</au><au>Suzuki, Toshikazu</au><au>Ayusawa, Dai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Release of Telomeric DNA from Chromosomes in Immortal Human Cells Lacking Telomerase Activity</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1998-07-20</date><risdate>1998</risdate><volume>248</volume><issue>2</issue><spage>223</spage><epage>227</epage><pages>223-227</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Some immortal human cell lines lack telomerase activity. These cell lines were found to contain small dispersed DNA hybridizing to TTAGGG repeats. Such DNA was located in their cytoplasm and nuclei. Normal human fibroblasts or telomerase-positive cell lines did not contain such DNA. Upon cloning and sequencing, it was shown to consist of TTAGGG repeats. When electrophoresed on neutral and alkaline agarose gels, it behaved as double-stranded and linear DNA. These results suggest that telomeric DNA is released from chromosomes in association with maintenance of telomeres in telomerase-negative cell lines.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9675117</pmid><doi>10.1006/bbrc.1998.8875</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1998-07, Vol.248 (2), p.223-227 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80031928 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Cell Line Chromosomes - genetics Cloning, Molecular DNA - chemistry DNA, Circular - genetics Electrophoresis, Agar Gel Humans Nucleic Acid Conformation Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid - genetics Sequence Analysis, DNA Telomere - genetics |
| title | Release of Telomeric DNA from Chromosomes in Immortal Human Cells Lacking Telomerase Activity |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T06%3A14%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Release%20of%20Telomeric%20DNA%20from%20Chromosomes%20in%20Immortal%20Human%20Cells%20Lacking%20Telomerase%20Activity&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Ogino,%20Hideki&rft.date=1998-07-20&rft.volume=248&rft.issue=2&rft.spage=223&rft.epage=227&rft.pages=223-227&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1006/bbrc.1998.8875&rft_dat=%3Cproquest_cross%3E16560352%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16560352&rft_id=info:pmid/9675117&rft_els_id=S0006291X98988751&rfr_iscdi=true |