Typing of Salmonella enteritidis of different phage types of PCR fingerprinting
Fifty-nine isolates of Salmonella spp. were typed by PCR fingerprinting using three single primers: ERIC2, M13 and OPS-19. First, their discrimination power in a group of nine different serotypes were studied and considerable differences in the band patterns were obtained. Further, a panel of 51 iso...
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Veröffentlicht in: | Journal of applied microbiology 1998-05, Vol.84 (5), p.877-882 |
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creator | Lopez-Molina, N Laconcha, I Rementeria, A Audicana, A Perales, I Garaizar, J |
description | Fifty-nine isolates of Salmonella spp. were typed by PCR fingerprinting using three single primers: ERIC2, M13 and OPS-19. First, their discrimination power in a group of nine different serotypes were studied and considerable differences in the band patterns were obtained. Further, a panel of 51 isolates of Salmonella enteritidis with eight different phage types were analysed with the three primers. The discriminating power increased by combining the patterns of the three primers, and in this case it was possible to distinguish between some phage types of Salm. enteritidis, but not all of them were differentiated. |
doi_str_mv | 10.1046/j.1365-2672.1998.00425.x |
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First, their discrimination power in a group of nine different serotypes were studied and considerable differences in the band patterns were obtained. Further, a panel of 51 isolates of Salmonella enteritidis with eight different phage types were analysed with the three primers. The discriminating power increased by combining the patterns of the three primers, and in this case it was possible to distinguish between some phage types of Salm. enteritidis, but not all of them were differentiated.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1046/j.1365-2672.1998.00425.x</identifier><identifier>PMID: 9674142</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Bacterial Typing Techniques ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Bacteriophage Typing ; bacteriophages ; Biological and medical sciences ; Disease Outbreaks ; DNA Fingerprinting - methods ; DNA, Bacterial - analysis ; Evaluation Studies as Topic ; Fundamental and applied biological sciences. 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The discriminating power increased by combining the patterns of the three primers, and in this case it was possible to distinguish between some phage types of Salm. enteritidis, but not all of them were differentiated.</description><subject>Bacterial Typing Techniques</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Bacteriophage Typing</subject><subject>bacteriophages</subject><subject>Biological and medical sciences</subject><subject>Disease Outbreaks</subject><subject>DNA Fingerprinting - methods</subject><subject>DNA, Bacterial - analysis</subject><subject>Evaluation Studies as Topic</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Polymerase Chain Reaction</topic><topic>Salmonella enteritidis - classification</topic><topic>Salmonella enteritidis - genetics</topic><topic>Salmonella Infections - epidemiology</topic><topic>Serotyping</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lopez-Molina, N</creatorcontrib><creatorcontrib>Laconcha, I</creatorcontrib><creatorcontrib>Rementeria, A</creatorcontrib><creatorcontrib>Audicana, A</creatorcontrib><creatorcontrib>Perales, I</creatorcontrib><creatorcontrib>Garaizar, J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lopez-Molina, N</au><au>Laconcha, I</au><au>Rementeria, A</au><au>Audicana, A</au><au>Perales, I</au><au>Garaizar, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Typing of Salmonella enteritidis of different phage types of PCR fingerprinting</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>1998-05-01</date><risdate>1998</risdate><volume>84</volume><issue>5</issue><spage>877</spage><epage>882</epage><pages>877-882</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Fifty-nine isolates of Salmonella spp. were typed by PCR fingerprinting using three single primers: ERIC2, M13 and OPS-19. 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source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
subjects | Bacterial Typing Techniques Bacteriological methods and techniques used in bacteriology Bacteriology Bacteriophage Typing bacteriophages Biological and medical sciences Disease Outbreaks DNA Fingerprinting - methods DNA, Bacterial - analysis Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology Humans Microbiology Polymerase Chain Reaction Salmonella enteritidis - classification Salmonella enteritidis - genetics Salmonella Infections - epidemiology Serotyping |
title | Typing of Salmonella enteritidis of different phage types of PCR fingerprinting |
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