Photoregulation of cot-1, a kinase-encoding gene involved in hyphal growth in Neurospora crassa
Blue light plays a key role as an environmental signal in the regulation of growth and development of fungi and plants. Here we demonstrate that in Neurospora crassa hyphae branch more frequently in cultures grown in light. Previous studies had identified cot-1 as a gene that controls apical hyphal...
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Veröffentlicht in: | Fungal genetics and biology 1998-04, Vol.23 (3), p.300-310 |
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creator | Lauter, F.R Marchfelder, U Russo, V.E.A Yamashiro, C.T Yatzkan, E Yarden, O |
description | Blue light plays a key role as an environmental signal in the regulation of growth and development of fungi and plants. Here we demonstrate that in Neurospora crassa hyphae branch more frequently in cultures grown in light. Previous studies had identified cot-1 as a gene that controls apical hyphal cell elongation. In the cot-1 mutant, cessation of elongation is accompanied by hyperbranching. Here we demonstrate that the cot-1 gene encodes two transcript species of about 2100 nt (cot-1 (s)) and about 2400 nt (cot-1 (l)) in length and that the ratio of both transcript species abundance is photoregulated. The origin of the difference between cot-1 (l) and cot-1 (s) was localized to the 5' end of the cot-1 transcripts, suggesting that two COT1 isoforms with different activities may be formed. Both light effects, on branching and on cot-1 expression, were dependent on functional wc-1 and wc-2 gene products. In addition to light, L-sorbose comprises another environmental cue that controls hyphal branching in N. crassa. In the presence of L-sorbose, photoregulation of cot-1 was blocked, suggesting the involvement of alternative and potentially interdependent signaling pathways for the regulation of hyphal elongation/branching. |
doi_str_mv | 10.1006/fgbi.1998.1038 |
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Here we demonstrate that in Neurospora crassa hyphae branch more frequently in cultures grown in light. Previous studies had identified cot-1 as a gene that controls apical hyphal cell elongation. In the cot-1 mutant, cessation of elongation is accompanied by hyperbranching. Here we demonstrate that the cot-1 gene encodes two transcript species of about 2100 nt (cot-1 (s)) and about 2400 nt (cot-1 (l)) in length and that the ratio of both transcript species abundance is photoregulated. The origin of the difference between cot-1 (l) and cot-1 (s) was localized to the 5' end of the cot-1 transcripts, suggesting that two COT1 isoforms with different activities may be formed. Both light effects, on branching and on cot-1 expression, were dependent on functional wc-1 and wc-2 gene products. In addition to light, L-sorbose comprises another environmental cue that controls hyphal branching in N. crassa. In the presence of L-sorbose, photoregulation of cot-1 was blocked, suggesting the involvement of alternative and potentially interdependent signaling pathways for the regulation of hyphal elongation/branching.</description><identifier>ISSN: 1087-1845</identifier><identifier>EISSN: 1096-0937</identifier><identifier>DOI: 10.1006/fgbi.1998.1038</identifier><identifier>PMID: 9680960</identifier><language>eng</language><publisher>United States</publisher><subject>amino acid sequences ; Base Sequence ; biochemical pathways ; Blotting, Northern ; blue light ; branches ; cell growth ; chromosome mapping ; fungal anatomy ; genbank/x97657 ; Gene Expression Regulation, Fungal ; genes ; Genes, Fungal ; genetic regulation ; growth ; hyphae ; isozymes ; Light ; messenger RNA ; Molecular Sequence Data ; morphogenesis ; Mutation ; Neurospora crassa ; Neurospora crassa - genetics ; Neurospora crassa - growth & development ; nucleotide sequences ; Plasmids ; Polymerase Chain Reaction ; Protein-Serine-Threonine Kinases - chemistry ; Protein-Serine-Threonine Kinases - genetics ; Protein-Serine-Threonine Kinases - metabolism ; ratios ; Sequence Analysis, DNA ; sorbose ; transcription (genetics) ; Transcription, Genetic</subject><ispartof>Fungal genetics and biology, 1998-04, Vol.23 (3), p.300-310</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9680960$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lauter, F.R</creatorcontrib><creatorcontrib>Marchfelder, U</creatorcontrib><creatorcontrib>Russo, V.E.A</creatorcontrib><creatorcontrib>Yamashiro, C.T</creatorcontrib><creatorcontrib>Yatzkan, E</creatorcontrib><creatorcontrib>Yarden, O</creatorcontrib><title>Photoregulation of cot-1, a kinase-encoding gene involved in hyphal growth in Neurospora crassa</title><title>Fungal genetics and biology</title><addtitle>Fungal Genet Biol</addtitle><description>Blue light plays a key role as an environmental signal in the regulation of growth and development of fungi and plants. Here we demonstrate that in Neurospora crassa hyphae branch more frequently in cultures grown in light. Previous studies had identified cot-1 as a gene that controls apical hyphal cell elongation. In the cot-1 mutant, cessation of elongation is accompanied by hyperbranching. Here we demonstrate that the cot-1 gene encodes two transcript species of about 2100 nt (cot-1 (s)) and about 2400 nt (cot-1 (l)) in length and that the ratio of both transcript species abundance is photoregulated. The origin of the difference between cot-1 (l) and cot-1 (s) was localized to the 5' end of the cot-1 transcripts, suggesting that two COT1 isoforms with different activities may be formed. Both light effects, on branching and on cot-1 expression, were dependent on functional wc-1 and wc-2 gene products. In addition to light, L-sorbose comprises another environmental cue that controls hyphal branching in N. crassa. In the presence of L-sorbose, photoregulation of cot-1 was blocked, suggesting the involvement of alternative and potentially interdependent signaling pathways for the regulation of hyphal elongation/branching.</description><subject>amino acid sequences</subject><subject>Base Sequence</subject><subject>biochemical pathways</subject><subject>Blotting, Northern</subject><subject>blue light</subject><subject>branches</subject><subject>cell growth</subject><subject>chromosome mapping</subject><subject>fungal anatomy</subject><subject>genbank/x97657</subject><subject>Gene Expression Regulation, Fungal</subject><subject>genes</subject><subject>Genes, Fungal</subject><subject>genetic regulation</subject><subject>growth</subject><subject>hyphae</subject><subject>isozymes</subject><subject>Light</subject><subject>messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>morphogenesis</subject><subject>Mutation</subject><subject>Neurospora crassa</subject><subject>Neurospora crassa - genetics</subject><subject>Neurospora crassa - growth & development</subject><subject>nucleotide sequences</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Protein-Serine-Threonine Kinases - chemistry</subject><subject>Protein-Serine-Threonine Kinases - genetics</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>ratios</subject><subject>Sequence Analysis, DNA</subject><subject>sorbose</subject><subject>transcription (genetics)</subject><subject>Transcription, Genetic</subject><issn>1087-1845</issn><issn>1096-0937</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kb1PwzAQxS0EKqWwsiE8IQZS_BXHHlHFl1QBEnSOnOSSGNI42ElR_3uC2pnp3t376Q3vEDqnZE4Jkbdlldk51VqNK1cHaEqJlhHRPDn80yqJqBLxMToJ4ZMQSmNBJ2iipRoxMkXpW-1656EaGtNb12JX4tz1Eb3BBn_Z1gSIoM1dYdsKV9ACtu3GNRsoRoHrbVebBlfe_fT13-EFBu9C57zBuTchmFN0VJomwNl-ztDq4f5j8RQtXx-fF3fLqGSS9xHTnBVCZTnneamoJkwmYCRLCmlACi0kLxgFmXEmlIJSZloUpmSZybUWGfAZutrldt59DxD6dG1DDk1jWnBDSBUhLOaxGMHrf0GqYk0l03E8ohd7dMjWUKSdt2vjt-m-vNG_3PmlcampvA3p6p0RyglTStPxH7_fwnpV</recordid><startdate>19980401</startdate><enddate>19980401</enddate><creator>Lauter, F.R</creator><creator>Marchfelder, U</creator><creator>Russo, V.E.A</creator><creator>Yamashiro, C.T</creator><creator>Yatzkan, E</creator><creator>Yarden, O</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19980401</creationdate><title>Photoregulation of cot-1, a kinase-encoding gene involved in hyphal growth in Neurospora crassa</title><author>Lauter, F.R ; Marchfelder, U ; Russo, V.E.A ; Yamashiro, C.T ; Yatzkan, E ; Yarden, O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f263t-2932d48bc33cf8190267ea627d6ae649463d21e6b32488ef6b94daf2bac994be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>amino acid sequences</topic><topic>Base Sequence</topic><topic>biochemical pathways</topic><topic>Blotting, Northern</topic><topic>blue light</topic><topic>branches</topic><topic>cell growth</topic><topic>chromosome mapping</topic><topic>fungal anatomy</topic><topic>genbank/x97657</topic><topic>Gene Expression Regulation, Fungal</topic><topic>genes</topic><topic>Genes, Fungal</topic><topic>genetic regulation</topic><topic>growth</topic><topic>hyphae</topic><topic>isozymes</topic><topic>Light</topic><topic>messenger RNA</topic><topic>Molecular Sequence Data</topic><topic>morphogenesis</topic><topic>Mutation</topic><topic>Neurospora crassa</topic><topic>Neurospora crassa - genetics</topic><topic>Neurospora crassa - growth & development</topic><topic>nucleotide sequences</topic><topic>Plasmids</topic><topic>Polymerase Chain Reaction</topic><topic>Protein-Serine-Threonine Kinases - chemistry</topic><topic>Protein-Serine-Threonine Kinases - genetics</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>ratios</topic><topic>Sequence Analysis, DNA</topic><topic>sorbose</topic><topic>transcription (genetics)</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lauter, F.R</creatorcontrib><creatorcontrib>Marchfelder, U</creatorcontrib><creatorcontrib>Russo, V.E.A</creatorcontrib><creatorcontrib>Yamashiro, C.T</creatorcontrib><creatorcontrib>Yatzkan, E</creatorcontrib><creatorcontrib>Yarden, O</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Fungal genetics and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lauter, F.R</au><au>Marchfelder, U</au><au>Russo, V.E.A</au><au>Yamashiro, C.T</au><au>Yatzkan, E</au><au>Yarden, O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photoregulation of cot-1, a kinase-encoding gene involved in hyphal growth in Neurospora crassa</atitle><jtitle>Fungal genetics and biology</jtitle><addtitle>Fungal Genet Biol</addtitle><date>1998-04-01</date><risdate>1998</risdate><volume>23</volume><issue>3</issue><spage>300</spage><epage>310</epage><pages>300-310</pages><issn>1087-1845</issn><eissn>1096-0937</eissn><abstract>Blue light plays a key role as an environmental signal in the regulation of growth and development of fungi and plants. Here we demonstrate that in Neurospora crassa hyphae branch more frequently in cultures grown in light. Previous studies had identified cot-1 as a gene that controls apical hyphal cell elongation. In the cot-1 mutant, cessation of elongation is accompanied by hyperbranching. Here we demonstrate that the cot-1 gene encodes two transcript species of about 2100 nt (cot-1 (s)) and about 2400 nt (cot-1 (l)) in length and that the ratio of both transcript species abundance is photoregulated. The origin of the difference between cot-1 (l) and cot-1 (s) was localized to the 5' end of the cot-1 transcripts, suggesting that two COT1 isoforms with different activities may be formed. Both light effects, on branching and on cot-1 expression, were dependent on functional wc-1 and wc-2 gene products. In addition to light, L-sorbose comprises another environmental cue that controls hyphal branching in N. crassa. In the presence of L-sorbose, photoregulation of cot-1 was blocked, suggesting the involvement of alternative and potentially interdependent signaling pathways for the regulation of hyphal elongation/branching.</abstract><cop>United States</cop><pmid>9680960</pmid><doi>10.1006/fgbi.1998.1038</doi><tpages>11</tpages></addata></record> |
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subjects | amino acid sequences Base Sequence biochemical pathways Blotting, Northern blue light branches cell growth chromosome mapping fungal anatomy genbank/x97657 Gene Expression Regulation, Fungal genes Genes, Fungal genetic regulation growth hyphae isozymes Light messenger RNA Molecular Sequence Data morphogenesis Mutation Neurospora crassa Neurospora crassa - genetics Neurospora crassa - growth & development nucleotide sequences Plasmids Polymerase Chain Reaction Protein-Serine-Threonine Kinases - chemistry Protein-Serine-Threonine Kinases - genetics Protein-Serine-Threonine Kinases - metabolism ratios Sequence Analysis, DNA sorbose transcription (genetics) Transcription, Genetic |
title | Photoregulation of cot-1, a kinase-encoding gene involved in hyphal growth in Neurospora crassa |
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