A comparison of flow cytometry and immunohistochemistry in human colorectal cancers
In human colorectal cancer it has been reported that some tumours lack the HLA‐ABC antigens. This has been interpreted as reflecting tumour escape from the immune system. Earlier data have been obtained by immunohistochemistry. In this study, we compared the expression of HLA‐ABC, HLA‐DR, CD80 (B7–1...
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| Veröffentlicht in: | APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 1998-01, Vol.106 (1-6), p.562-570 |
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| creator | DIEDERICHSEN, AXEL COSMUS PYNDT HANSEN, TINE PLATO NIELSEN, OLE FENGER, CLAUS JENSENIUS, JENS CHRISTIAN CHRISTENSEN, PER BORUP KRISTENSEN, TOM ZEUTHEN, JESPER |
| description | In human colorectal cancer it has been reported that some tumours lack the HLA‐ABC antigens. This has been interpreted as reflecting tumour escape from the immune system. Earlier data have been obtained by immunohistochemistry. In this study, we compared the expression of HLA‐ABC, HLA‐DR, CD80 (B7–1) and CD54 (ICAM‐1) in 20 tumours using both a conventional immunohistochemistry two‐layer technique and multiparameter flow cytometry, gating on an epithelial cell marker. Colorectal cancer tissue used in flow cytometry was dissociated with collagenase, deoxyribonuclease and hyaluronidase. The intensity of expression of HLA‐ABC, HLA‐DR and CD80 was unaffected by the enzymes, but CD54 was decreased by 30%. The reproducibility of flow cytometry was good. Microscopy of sections revealed that about 5% of each tumour sample consisted of normal epithelium, but even after correction for this, flow cytometry was superior to immunohistochemistry in 33 out of 80 cases, and showed that tumours described as HLA‐ABC negative by immunohistochemistry were in fact weakly positive for HLA‐ABC. We conclude that flow cytometry and immunohistochemistry are complementary, and that flow cytometry is superior to immunohistochemistry for detecting anti‐gens/epitopes present in low amounts. |
| doi_str_mv | 10.1111/j.1699-0463.1998.tb01385.x |
| format | Article |
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This has been interpreted as reflecting tumour escape from the immune system. Earlier data have been obtained by immunohistochemistry. In this study, we compared the expression of HLA‐ABC, HLA‐DR, CD80 (B7–1) and CD54 (ICAM‐1) in 20 tumours using both a conventional immunohistochemistry two‐layer technique and multiparameter flow cytometry, gating on an epithelial cell marker. Colorectal cancer tissue used in flow cytometry was dissociated with collagenase, deoxyribonuclease and hyaluronidase. The intensity of expression of HLA‐ABC, HLA‐DR and CD80 was unaffected by the enzymes, but CD54 was decreased by 30%. The reproducibility of flow cytometry was good. Microscopy of sections revealed that about 5% of each tumour sample consisted of normal epithelium, but even after correction for this, flow cytometry was superior to immunohistochemistry in 33 out of 80 cases, and showed that tumours described as HLA‐ABC negative by immunohistochemistry were in fact weakly positive for HLA‐ABC. We conclude that flow cytometry and immunohistochemistry are complementary, and that flow cytometry is superior to immunohistochemistry for detecting anti‐gens/epitopes present in low amounts.</description><identifier>ISSN: 0903-4641</identifier><identifier>EISSN: 1600-0463</identifier><identifier>DOI: 10.1111/j.1699-0463.1998.tb01385.x</identifier><identifier>PMID: 9674894</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject><![CDATA[Aged ; Aged, 80 and over ; Antigens, Neoplasm - isolation & purification ; B7-1 Antigen - isolation & purification ; Biological and medical sciences ; colorectal cancer ; Colorectal Neoplasms - immunology ; Digestive system ; Female ; Flow cytometry ; Flow Cytometry - methods ; HLA Antigens - isolation & purification ; HLA-A Antigens - isolation & purification ; HLA-ABC ; HLA-B Antigens - isolation & purification ; HLA-C Antigens - isolation & purification ; HLA-DR ; HLA-DR Antigens - isolation & purification ; Humans ; immunohistochemistry ; Immunohistochemistry - methods ; Intercellular Adhesion Molecule-1 - isolation & purification ; Investigative techniques, diagnostic techniques (general aspects) ; Male ; Medical sciences ; Middle Aged ; Pathology. 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Miscellaneous investigative techniques ; Reproducibility of Results ; sensitivity]]></subject><ispartof>APMIS : acta pathologica, microbiologica et immunologica Scandinavica, 1998-01, Vol.106 (1-6), p.562-570</ispartof><rights>1998 APMIS</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4362-b4b55605ba6954d4a4742a434ec9a9ff31de8dfb9c13cff316d98ef0fa2bc2b03</citedby><cites>FETCH-LOGICAL-c4362-b4b55605ba6954d4a4742a434ec9a9ff31de8dfb9c13cff316d98ef0fa2bc2b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1699-0463.1998.tb01385.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1699-0463.1998.tb01385.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2344019$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9674894$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DIEDERICHSEN, AXEL COSMUS PYNDT</creatorcontrib><creatorcontrib>HANSEN, TINE PLATO</creatorcontrib><creatorcontrib>NIELSEN, OLE</creatorcontrib><creatorcontrib>FENGER, CLAUS</creatorcontrib><creatorcontrib>JENSENIUS, JENS CHRISTIAN</creatorcontrib><creatorcontrib>CHRISTENSEN, PER BORUP</creatorcontrib><creatorcontrib>KRISTENSEN, TOM</creatorcontrib><creatorcontrib>ZEUTHEN, JESPER</creatorcontrib><title>A comparison of flow cytometry and immunohistochemistry in human colorectal cancers</title><title>APMIS : acta pathologica, microbiologica et immunologica Scandinavica</title><addtitle>APMIS</addtitle><description>In human colorectal cancer it has been reported that some tumours lack the HLA‐ABC antigens. This has been interpreted as reflecting tumour escape from the immune system. Earlier data have been obtained by immunohistochemistry. In this study, we compared the expression of HLA‐ABC, HLA‐DR, CD80 (B7–1) and CD54 (ICAM‐1) in 20 tumours using both a conventional immunohistochemistry two‐layer technique and multiparameter flow cytometry, gating on an epithelial cell marker. Colorectal cancer tissue used in flow cytometry was dissociated with collagenase, deoxyribonuclease and hyaluronidase. The intensity of expression of HLA‐ABC, HLA‐DR and CD80 was unaffected by the enzymes, but CD54 was decreased by 30%. The reproducibility of flow cytometry was good. Microscopy of sections revealed that about 5% of each tumour sample consisted of normal epithelium, but even after correction for this, flow cytometry was superior to immunohistochemistry in 33 out of 80 cases, and showed that tumours described as HLA‐ABC negative by immunohistochemistry were in fact weakly positive for HLA‐ABC. We conclude that flow cytometry and immunohistochemistry are complementary, and that flow cytometry is superior to immunohistochemistry for detecting anti‐gens/epitopes present in low amounts.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antigens, Neoplasm - isolation & purification</subject><subject>B7-1 Antigen - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>colorectal cancer</subject><subject>Colorectal Neoplasms - immunology</subject><subject>Digestive system</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Flow Cytometry - methods</subject><subject>HLA Antigens - isolation & purification</subject><subject>HLA-A Antigens - isolation & purification</subject><subject>HLA-ABC</subject><subject>HLA-B Antigens - isolation & purification</subject><subject>HLA-C Antigens - isolation & purification</subject><subject>HLA-DR</subject><subject>HLA-DR Antigens - isolation & purification</subject><subject>Humans</subject><subject>immunohistochemistry</subject><subject>Immunohistochemistry - methods</subject><subject>Intercellular Adhesion Molecule-1 - isolation & purification</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Reproducibility of Results</subject><subject>sensitivity</subject><issn>0903-4641</issn><issn>1600-0463</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkF1r2zAUhsXoaLNuP6FgRtmdXX1ZlnpRCKHtVtKs0JZdClmWiDPbyiSbJP9-8mJyP90cxPtxDg8AXxHMUHw3mwwxIVJIGcmQEDzrS4gIz7P9BzBDDMJ_0hmYQQFJShlFF-BTCBsIEeasOAfnghWUCzoDr_NEu3arfB1clzib2MbtEn3oXWt6f0hUVyV12w6dW9ehd3pt2jijUHfJemhVF-ON80b3qkm06rTx4TP4aFUTzJdpXoL3h_u3xfd0-fPxx2K-TDUlDKclLfOcwbxUTOS0oooWFCtKqNFCCWsJqgyvbCk0Inr8skpwY6FVuNS4hOQSfDv2br37M5jQy3ibNk2jOuOGIDmEGCPOovH2aNTeheCNlVtft8ofJIJyJCo3ciQqR2xyJCononIfw1fTlqFsTXWKTgijfj3pKmjVWB8h1OFkw4RSiES03R1tu7oxh_84QM5fnnOGY0F6LIj8zf5UoPxvyQpS5PLX6lGunhYr-syXkpC_JFSj5g</recordid><startdate>199801</startdate><enddate>199801</enddate><creator>DIEDERICHSEN, AXEL COSMUS PYNDT</creator><creator>HANSEN, TINE PLATO</creator><creator>NIELSEN, OLE</creator><creator>FENGER, CLAUS</creator><creator>JENSENIUS, JENS CHRISTIAN</creator><creator>CHRISTENSEN, PER BORUP</creator><creator>KRISTENSEN, TOM</creator><creator>ZEUTHEN, JESPER</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199801</creationdate><title>A comparison of flow cytometry and immunohistochemistry in human colorectal cancers</title><author>DIEDERICHSEN, AXEL COSMUS PYNDT ; HANSEN, TINE PLATO ; NIELSEN, OLE ; FENGER, CLAUS ; JENSENIUS, JENS CHRISTIAN ; CHRISTENSEN, PER BORUP ; KRISTENSEN, TOM ; ZEUTHEN, JESPER</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4362-b4b55605ba6954d4a4742a434ec9a9ff31de8dfb9c13cff316d98ef0fa2bc2b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antigens, Neoplasm - isolation & purification</topic><topic>B7-1 Antigen - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>colorectal cancer</topic><topic>Colorectal Neoplasms - immunology</topic><topic>Digestive system</topic><topic>Female</topic><topic>Flow cytometry</topic><topic>Flow Cytometry - methods</topic><topic>HLA Antigens - isolation & purification</topic><topic>HLA-A Antigens - isolation & purification</topic><topic>HLA-ABC</topic><topic>HLA-B Antigens - isolation & purification</topic><topic>HLA-C Antigens - isolation & purification</topic><topic>HLA-DR</topic><topic>HLA-DR Antigens - isolation & purification</topic><topic>Humans</topic><topic>immunohistochemistry</topic><topic>Immunohistochemistry - methods</topic><topic>Intercellular Adhesion Molecule-1 - isolation & purification</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Pathology. 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This has been interpreted as reflecting tumour escape from the immune system. Earlier data have been obtained by immunohistochemistry. In this study, we compared the expression of HLA‐ABC, HLA‐DR, CD80 (B7–1) and CD54 (ICAM‐1) in 20 tumours using both a conventional immunohistochemistry two‐layer technique and multiparameter flow cytometry, gating on an epithelial cell marker. Colorectal cancer tissue used in flow cytometry was dissociated with collagenase, deoxyribonuclease and hyaluronidase. The intensity of expression of HLA‐ABC, HLA‐DR and CD80 was unaffected by the enzymes, but CD54 was decreased by 30%. The reproducibility of flow cytometry was good. Microscopy of sections revealed that about 5% of each tumour sample consisted of normal epithelium, but even after correction for this, flow cytometry was superior to immunohistochemistry in 33 out of 80 cases, and showed that tumours described as HLA‐ABC negative by immunohistochemistry were in fact weakly positive for HLA‐ABC. We conclude that flow cytometry and immunohistochemistry are complementary, and that flow cytometry is superior to immunohistochemistry for detecting anti‐gens/epitopes present in low amounts.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9674894</pmid><doi>10.1111/j.1699-0463.1998.tb01385.x</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0903-4641 |
| ispartof | APMIS : acta pathologica, microbiologica et immunologica Scandinavica, 1998-01, Vol.106 (1-6), p.562-570 |
| issn | 0903-4641 1600-0463 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Aged Aged, 80 and over Antigens, Neoplasm - isolation & purification B7-1 Antigen - isolation & purification Biological and medical sciences colorectal cancer Colorectal Neoplasms - immunology Digestive system Female Flow cytometry Flow Cytometry - methods HLA Antigens - isolation & purification HLA-A Antigens - isolation & purification HLA-ABC HLA-B Antigens - isolation & purification HLA-C Antigens - isolation & purification HLA-DR HLA-DR Antigens - isolation & purification Humans immunohistochemistry Immunohistochemistry - methods Intercellular Adhesion Molecule-1 - isolation & purification Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Middle Aged Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Reproducibility of Results sensitivity |
| title | A comparison of flow cytometry and immunohistochemistry in human colorectal cancers |
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