The mannitol utilization genes of Pseudomonas fluorescens are regulated by an activator: Cloning, nucleotide sequence and expression of the mtlR gene

A plasmid with the galK gene under control of the promoter of the mannitol utilization genes ( mtl) from Pseudomonas fluorescens DSM 50106 was constructed to isolate the mtl regulatory gene. An Escherichia coli galK − mtl − strain with this plasmid was used to screen a genomic library of P. fluoresc...

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Veröffentlicht in:	Gene 1998-07, Vol.215 (1), p.19-27
Hauptverfasser:	Brünker, Peter, Hils, Martin, Altenbuchner, Josef, Mattes, Ralf
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Proteins - physiology Cloning, Molecular DNA, Bacterial - genetics DNA, Bacterial - isolation & purification DNA, Bacterial - metabolism DNA-Binding Proteins - metabolism Escherichia coli Proteins Gene Expression - genetics Gene Expression Regulation, Bacterial Genes, Bacterial - genetics Mannitol - metabolism Molecular Sequence Data Polyols Promoter probe vector Protein Binding Pseudomonas fluorescens - chemistry Pseudomonas fluorescens - genetics Regulation Repressor Proteins - genetics Repressor Proteins - physiology Restriction Mapping Sequence Analysis, DNA Sequence Homology, Amino Acid Trans-Activators - genetics Transcription activator XylS/AraC family
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