Transforming growth-factor-β (TGF-β) inhibits albumin synthesis in normal human hepatocytes and in hepatoma HepG2 cells
We explored the effect of transforming growth factor β (TGF-β), a cytokine that appears to play a central role in inflammatory events, on albumin expression by normal adult human hepatocytes and hepatoma cells. Addition of TGF-β to primary human hepatocyte cultures resulted in a dramatic decrease in...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1990-09, Vol.171 (2), p.647-654 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Busso, Nathalie Chesne, Christophe Delers, François Morel, Fabrice Guillouzo, André |
| description | We explored the effect of transforming growth factor β (TGF-β), a cytokine that appears to play a central role in inflammatory events, on albumin expression by normal adult human hepatocytes and hepatoma cells. Addition of TGF-β to primary human hepatocyte cultures resulted in a dramatic decrease in albumin accumulation and synthesis. This effect was dose-dependent, took place after a 48h incubation period and was maintained over 96h. TGF-β-decreased albumin protein levels were associated with reduced albumin mRNA content. Actin mRNA levels were concomittantly increased. Comparable qualitative effects of TGF-β were observed on human hepatoma HepG2 cells. |
| doi_str_mv | 10.1016/0006-291X(90)91195-X |
| format | Article |
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Addition of TGF-β to primary human hepatocyte cultures resulted in a dramatic decrease in albumin accumulation and synthesis. This effect was dose-dependent, took place after a 48h incubation period and was maintained over 96h. TGF-β-decreased albumin protein levels were associated with reduced albumin mRNA content. Actin mRNA levels were concomittantly increased. Comparable qualitative effects of TGF-β were observed on human hepatoma HepG2 cells.</description><subject>Adult</subject><subject>Carcinoma, Hepatocellular</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>DNA Probes</subject><subject>Humans</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Liver Neoplasms</subject><subject>Male</subject><subject>Molecular Weight</subject><subject>Recombinant Proteins - pharmacology</subject><subject>RNA, Messenger - drug effects</subject><subject>RNA, Messenger - genetics</subject><subject>Serum Albumin - biosynthesis</subject><subject>Serum Albumin - genetics</subject><subject>Serum Albumin - isolation & purification</subject><subject>Transcription, Genetic</subject><subject>Transforming Growth Factors - pharmacology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdFKwzAYhYMoOqdvoJArmRfVpE3T5kaQ4aYw8GbC7kKapjbSpjNJlb2WD-IzmbqxS736Iec7f37OAeACoxuMML1FCNEoZng1YeiaYczSaHUARhgxFMUYkUMw2iMn4NS5N4QwJpQdg-MYU5YlaAQ2SyuMqzrbavMKX2336euoEtJ3Nvr-gpPlfBbmNdSm1oX2Doqm6AML3cb4WjntggRN8IsG1n0rDKzVWvhObrwKtCkHffvUCvio1vMYStU07gwcVaJx6nw3x-Bl9rCcPkaL5_nT9H4RSZIwH6VFLAliLGNEEpLlZVHJvJI4XJ-yLMOpzLMqlqmQOWEJpZSoMktEWkpBSUVRMgZX271r2733ynneajdcIIzqesfzkArJMfkXxCmLaRoPINmC0nbOWVXxtdWtsBuOER-q4UPufMidM8R_q-GrYLvc7e-LVpV7066LoN9tdRXS-NDKcie1MlKV2irpednpvz_4AeTGn88</recordid><startdate>19900914</startdate><enddate>19900914</enddate><creator>Busso, Nathalie</creator><creator>Chesne, Christophe</creator><creator>Delers, François</creator><creator>Morel, Fabrice</creator><creator>Guillouzo, André</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19900914</creationdate><title>Transforming growth-factor-β (TGF-β) inhibits albumin synthesis in normal human hepatocytes and in hepatoma HepG2 cells</title><author>Busso, Nathalie ; Chesne, Christophe ; Delers, François ; Morel, Fabrice ; Guillouzo, André</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-5b2c4099794c4478dbfc8fc1730597715c87f2c5ac84936664ed73a5dca64f603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Adult</topic><topic>Carcinoma, Hepatocellular</topic><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>DNA Probes</topic><topic>Humans</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Liver Neoplasms</topic><topic>Male</topic><topic>Molecular Weight</topic><topic>Recombinant Proteins - pharmacology</topic><topic>RNA, Messenger - drug effects</topic><topic>RNA, Messenger - genetics</topic><topic>Serum Albumin - biosynthesis</topic><topic>Serum Albumin - genetics</topic><topic>Serum Albumin - isolation & purification</topic><topic>Transcription, Genetic</topic><topic>Transforming Growth Factors - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Busso, Nathalie</creatorcontrib><creatorcontrib>Chesne, Christophe</creatorcontrib><creatorcontrib>Delers, François</creatorcontrib><creatorcontrib>Morel, Fabrice</creatorcontrib><creatorcontrib>Guillouzo, André</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Busso, Nathalie</au><au>Chesne, Christophe</au><au>Delers, François</au><au>Morel, Fabrice</au><au>Guillouzo, André</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transforming growth-factor-β (TGF-β) inhibits albumin synthesis in normal human hepatocytes and in hepatoma HepG2 cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1990-09-14</date><risdate>1990</risdate><volume>171</volume><issue>2</issue><spage>647</spage><epage>654</epage><pages>647-654</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>We explored the effect of transforming growth factor β (TGF-β), a cytokine that appears to play a central role in inflammatory events, on albumin expression by normal adult human hepatocytes and hepatoma cells. Addition of TGF-β to primary human hepatocyte cultures resulted in a dramatic decrease in albumin accumulation and synthesis. This effect was dose-dependent, took place after a 48h incubation period and was maintained over 96h. TGF-β-decreased albumin protein levels were associated with reduced albumin mRNA content. Actin mRNA levels were concomittantly increased. Comparable qualitative effects of TGF-β were observed on human hepatoma HepG2 cells.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>2169730</pmid><doi>10.1016/0006-291X(90)91195-X</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1990-09, Vol.171 (2), p.647-654 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Carcinoma, Hepatocellular Cell Line Cells, Cultured DNA Probes Humans Liver - drug effects Liver - metabolism Liver Neoplasms Male Molecular Weight Recombinant Proteins - pharmacology RNA, Messenger - drug effects RNA, Messenger - genetics Serum Albumin - biosynthesis Serum Albumin - genetics Serum Albumin - isolation & purification Transcription, Genetic Transforming Growth Factors - pharmacology |
| title | Transforming growth-factor-β (TGF-β) inhibits albumin synthesis in normal human hepatocytes and in hepatoma HepG2 cells |
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