High performance liquid chromatographic measurement of bisoprolol in plasma

High performance liquid chromatographic measurement of bisoprolol in plasma

A simple high performance liquid chromatographic method has been devised for the measurement of bisoprolol in plasma or serum. The sample (200 μL) is vortex mixed for 30 s with 2 M Tris solution (50 μL), aqueous internal standard (benzimidazole, 2.0 mg/L, 50 μL) and methyl t‐butyl ether (200 μL). Af...

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Veröffentlicht in:Biomedical chromatography 1990-07, Vol.4 (4), p.178-180
Hauptverfasser: Eastwood, R. J., Jerman, J. C., Bhamra, R. K., Holt, D. W.
Format: Artikel
Sprache:eng
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Bisoprolol
Chromatography, High Pressure Liquid
Humans
Indicators and Reagents
Propanolamines - blood
Spectrometry, Fluorescence
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container_end_page 180
container_issue 4
container_start_page 178
container_title Biomedical chromatography
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creator Eastwood, R. J.
Jerman, J. C.
Bhamra, R. K.
Holt, D. W.
description A simple high performance liquid chromatographic method has been devised for the measurement of bisoprolol in plasma or serum. The sample (200 μL) is vortex mixed for 30 s with 2 M Tris solution (50 μL), aqueous internal standard (benzimidazole, 2.0 mg/L, 50 μL) and methyl t‐butyl ether (200 μL). After centrifugation (9950 × g, 2 min), a portion of the resulting extract is analysed on a microparticulate (5 μm) silica column using 1 mM camphorsulphonic acid in methanol as the mobile phase. Detection is by fluorescence at an excitatioh wavelength of 215 nM. The lower limit of accurate measurement for the assay is 10 μ/L (CV% = 8.9, n = 9) with a lower limit of detection of 5 μg/L. There is minimal interference from either commonly prescribed drugs or endogenous compounds.
doi_str_mv 10.1002/bmc.1130040415
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subjects Bisoprolol
Chromatography, High Pressure Liquid
Humans
Indicators and Reagents
Propanolamines - blood
Spectrometry, Fluorescence
title High performance liquid chromatographic measurement of bisoprolol in plasma
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