Detection of infectious pancreatic necrosis virus (IPNV) RNA by hybridization with an oligonucleotide DNA probe
Marine farming of Atlantic salmon ( Salmo salar) is growing rapidly in Norway, and fish diseases have now become one of the biggest obstacles for this new industry. Infectious pancreatic necrosis virus (IPNV) is commonly found in fish from Norwegian sea farms. Diagnosis of IPNV infection is, at pres...
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| Veröffentlicht in: | Veterinary microbiology 1990-06, Vol.23 (1), p.211-219 |
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| creator | Rimstad, E. Krona, R. Hornes, E. Olsvik, Ø. Hyllseth, B. |
| description | Marine farming of Atlantic salmon (
Salmo salar) is growing rapidly in Norway, and fish diseases have now become one of the biggest obstacles for this new industry. Infectious pancreatic necrosis virus (IPNV) is commonly found in fish from Norwegian sea farms. Diagnosis of IPNV infection is, at present, mainly based on virus isolation in cell cultures and identification by neutralization tests (NT). A DNA-RNA hybridization assay was developed using a 24 base DNA oligonucleotide probe. This is homologous to a part of the nucleotide sequence of the IPNV genome coding for a protease. RNA extracted from IPNV and harvest from infected cell cultures were fixed to nylon filters and hybridized with the
32P end-labelled probe. The results showed that the probe specifically identified IPNV from these two materials, both for the three different virus strains (Ab, Sp and VR-299) used, and for several different field isolates. It did not hybridize with reoviruses or non-infected cell cultures used as controls. These results indicate that the probe is not serotype specific, and furthermore that RNA extraction is not required before hybridization. This method may be a useful alternative to NT for routine identification of IPNV, particularly when non-radioactive labelling of the probe is introduced. |
| doi_str_mv | 10.1016/0378-1135(90)90151-K |
| format | Article |
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Salmo salar) is growing rapidly in Norway, and fish diseases have now become one of the biggest obstacles for this new industry. Infectious pancreatic necrosis virus (IPNV) is commonly found in fish from Norwegian sea farms. Diagnosis of IPNV infection is, at present, mainly based on virus isolation in cell cultures and identification by neutralization tests (NT). A DNA-RNA hybridization assay was developed using a 24 base DNA oligonucleotide probe. This is homologous to a part of the nucleotide sequence of the IPNV genome coding for a protease. RNA extracted from IPNV and harvest from infected cell cultures were fixed to nylon filters and hybridized with the
32P end-labelled probe. The results showed that the probe specifically identified IPNV from these two materials, both for the three different virus strains (Ab, Sp and VR-299) used, and for several different field isolates. It did not hybridize with reoviruses or non-infected cell cultures used as controls. These results indicate that the probe is not serotype specific, and furthermore that RNA extraction is not required before hybridization. This method may be a useful alternative to NT for routine identification of IPNV, particularly when non-radioactive labelling of the probe is introduced.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/0378-1135(90)90151-K</identifier><identifier>PMID: 2169675</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Autoradiography ; Brackish ; disease detection ; DNA Probes ; FISH CULTURE ; fish diseases ; Fish Diseases - diagnosis ; Fish Diseases - microbiology ; Freshwater ; hybridization ; hybridization analysis ; IDENTIFICACION ; IDENTIFICATION ; infections diseases ; Marine ; Nucleic Acid Hybridization ; Oligonucleotide Probes ; oligonucleotides ; PANCREATIC NECROSIS VIRUS ; PISCICULTURA ; PISCICULTURE ; probes ; Reoviridae - genetics ; Reoviridae - isolation & purification ; Reoviridae Infections - diagnosis ; Reoviridae Infections - microbiology ; Reoviridae Infections - veterinary ; RNA, Viral - analysis ; SALMO SALAR ; Salmon ; viral diseases ; VIRUS NECROSE PANCREATIQUE ; VIRUS NECROSIS PANCREATICA ; viruses</subject><ispartof>Veterinary microbiology, 1990-06, Vol.23 (1), p.211-219</ispartof><rights>1990</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-53f2e72e15740c048fab54def3d32c70af4ac1dd7668ee4b151b723ca4a4d583</citedby><cites>FETCH-LOGICAL-c407t-53f2e72e15740c048fab54def3d32c70af4ac1dd7668ee4b151b723ca4a4d583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1135(90)90151-K$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2169675$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Pastoret, P-P (eds)</contributor><contributor>Edwards, S</contributor><creatorcontrib>Rimstad, E.</creatorcontrib><creatorcontrib>Krona, R.</creatorcontrib><creatorcontrib>Hornes, E.</creatorcontrib><creatorcontrib>Olsvik, Ø.</creatorcontrib><creatorcontrib>Hyllseth, B.</creatorcontrib><title>Detection of infectious pancreatic necrosis virus (IPNV) RNA by hybridization with an oligonucleotide DNA probe</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Marine farming of Atlantic salmon (
Salmo salar) is growing rapidly in Norway, and fish diseases have now become one of the biggest obstacles for this new industry. Infectious pancreatic necrosis virus (IPNV) is commonly found in fish from Norwegian sea farms. Diagnosis of IPNV infection is, at present, mainly based on virus isolation in cell cultures and identification by neutralization tests (NT). A DNA-RNA hybridization assay was developed using a 24 base DNA oligonucleotide probe. This is homologous to a part of the nucleotide sequence of the IPNV genome coding for a protease. RNA extracted from IPNV and harvest from infected cell cultures were fixed to nylon filters and hybridized with the
32P end-labelled probe. The results showed that the probe specifically identified IPNV from these two materials, both for the three different virus strains (Ab, Sp and VR-299) used, and for several different field isolates. It did not hybridize with reoviruses or non-infected cell cultures used as controls. These results indicate that the probe is not serotype specific, and furthermore that RNA extraction is not required before hybridization. This method may be a useful alternative to NT for routine identification of IPNV, particularly when non-radioactive labelling of the probe is introduced.</description><subject>Animals</subject><subject>Autoradiography</subject><subject>Brackish</subject><subject>disease detection</subject><subject>DNA Probes</subject><subject>FISH CULTURE</subject><subject>fish diseases</subject><subject>Fish Diseases - diagnosis</subject><subject>Fish Diseases - microbiology</subject><subject>Freshwater</subject><subject>hybridization</subject><subject>hybridization analysis</subject><subject>IDENTIFICACION</subject><subject>IDENTIFICATION</subject><subject>infections diseases</subject><subject>Marine</subject><subject>Nucleic Acid Hybridization</subject><subject>Oligonucleotide Probes</subject><subject>oligonucleotides</subject><subject>PANCREATIC NECROSIS VIRUS</subject><subject>PISCICULTURA</subject><subject>PISCICULTURE</subject><subject>probes</subject><subject>Reoviridae - genetics</subject><subject>Reoviridae - isolation & purification</subject><subject>Reoviridae Infections - diagnosis</subject><subject>Reoviridae Infections - microbiology</subject><subject>Reoviridae Infections - veterinary</subject><subject>RNA, Viral - analysis</subject><subject>SALMO SALAR</subject><subject>Salmon</subject><subject>viral diseases</subject><subject>VIRUS NECROSE PANCREATIQUE</subject><subject>VIRUS NECROSIS PANCREATICA</subject><subject>viruses</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUctOHDEQtKIgspD8QJRIPkVwmMT2-DFziYR4JIjVgiKUq-Wx2-BodrzYs6Dl6_E-xJGcWq2qru6uQugLJd8pofIHqVVTUVqLo5Yct4QKWl29QxPaqLpigrP3aPJK-YAOcv5HCOGtJPton1HZSiUmKJ7BCHYMccDR4zD4TbPMeGEGm8CMweIBbIo5ZPwYUkGOLm9mf4_xn9kJ7lb4ftWl4MKz2Wg8hfEem6LVh7s4LG0PcQwO8FkhL1Ls4CPa86bP8GlXD9Htxfnt6e9qev3r8vRkWllO1FiJ2jNQDKhQnFjCG286wR342tXMKmI8N5Y6p6RsAHhXnu8Uq63hhjvR1Ifo21a2LH1YQh71PGQLfW8GKN_pplghmZD_JVJJGJdcFSLfEtde5AReL1KYm7TSlOh1Hnpttl6brVuiN3noqzL2dae_7ObgXod2ART88xb3Jmpzl0LWs2lLy3EtK-DPLQjFqccASWcbYLDgQio5aRfD29tfAKgJonY</recordid><startdate>19900601</startdate><enddate>19900601</enddate><creator>Rimstad, E.</creator><creator>Krona, R.</creator><creator>Hornes, E.</creator><creator>Olsvik, Ø.</creator><creator>Hyllseth, B.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>H98</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19900601</creationdate><title>Detection of infectious pancreatic necrosis virus (IPNV) RNA by hybridization with an oligonucleotide DNA probe</title><author>Rimstad, E. ; Krona, R. ; Hornes, E. ; Olsvik, Ø. ; Hyllseth, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-53f2e72e15740c048fab54def3d32c70af4ac1dd7668ee4b151b723ca4a4d583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Autoradiography</topic><topic>Brackish</topic><topic>disease detection</topic><topic>DNA Probes</topic><topic>FISH CULTURE</topic><topic>fish diseases</topic><topic>Fish Diseases - diagnosis</topic><topic>Fish Diseases - microbiology</topic><topic>Freshwater</topic><topic>hybridization</topic><topic>hybridization analysis</topic><topic>IDENTIFICACION</topic><topic>IDENTIFICATION</topic><topic>infections diseases</topic><topic>Marine</topic><topic>Nucleic Acid Hybridization</topic><topic>Oligonucleotide Probes</topic><topic>oligonucleotides</topic><topic>PANCREATIC NECROSIS VIRUS</topic><topic>PISCICULTURA</topic><topic>PISCICULTURE</topic><topic>probes</topic><topic>Reoviridae - genetics</topic><topic>Reoviridae - isolation & purification</topic><topic>Reoviridae Infections - diagnosis</topic><topic>Reoviridae Infections - microbiology</topic><topic>Reoviridae Infections - veterinary</topic><topic>RNA, Viral - analysis</topic><topic>SALMO SALAR</topic><topic>Salmon</topic><topic>viral diseases</topic><topic>VIRUS NECROSE PANCREATIQUE</topic><topic>VIRUS NECROSIS PANCREATICA</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rimstad, E.</creatorcontrib><creatorcontrib>Krona, R.</creatorcontrib><creatorcontrib>Hornes, E.</creatorcontrib><creatorcontrib>Olsvik, Ø.</creatorcontrib><creatorcontrib>Hyllseth, B.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rimstad, E.</au><au>Krona, R.</au><au>Hornes, E.</au><au>Olsvik, Ø.</au><au>Hyllseth, B.</au><au>Pastoret, P-P (eds)</au><au>Edwards, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of infectious pancreatic necrosis virus (IPNV) RNA by hybridization with an oligonucleotide DNA probe</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>1990-06-01</date><risdate>1990</risdate><volume>23</volume><issue>1</issue><spage>211</spage><epage>219</epage><pages>211-219</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><abstract>Marine farming of Atlantic salmon (
Salmo salar) is growing rapidly in Norway, and fish diseases have now become one of the biggest obstacles for this new industry. Infectious pancreatic necrosis virus (IPNV) is commonly found in fish from Norwegian sea farms. Diagnosis of IPNV infection is, at present, mainly based on virus isolation in cell cultures and identification by neutralization tests (NT). A DNA-RNA hybridization assay was developed using a 24 base DNA oligonucleotide probe. This is homologous to a part of the nucleotide sequence of the IPNV genome coding for a protease. RNA extracted from IPNV and harvest from infected cell cultures were fixed to nylon filters and hybridized with the
32P end-labelled probe. The results showed that the probe specifically identified IPNV from these two materials, both for the three different virus strains (Ab, Sp and VR-299) used, and for several different field isolates. It did not hybridize with reoviruses or non-infected cell cultures used as controls. These results indicate that the probe is not serotype specific, and furthermore that RNA extraction is not required before hybridization. This method may be a useful alternative to NT for routine identification of IPNV, particularly when non-radioactive labelling of the probe is introduced.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>2169675</pmid><doi>10.1016/0378-1135(90)90151-K</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1135 |
| ispartof | Veterinary microbiology, 1990-06, Vol.23 (1), p.211-219 |
| issn | 0378-1135 1873-2542 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Autoradiography Brackish disease detection DNA Probes FISH CULTURE fish diseases Fish Diseases - diagnosis Fish Diseases - microbiology Freshwater hybridization hybridization analysis IDENTIFICACION IDENTIFICATION infections diseases Marine Nucleic Acid Hybridization Oligonucleotide Probes oligonucleotides PANCREATIC NECROSIS VIRUS PISCICULTURA PISCICULTURE probes Reoviridae - genetics Reoviridae - isolation & purification Reoviridae Infections - diagnosis Reoviridae Infections - microbiology Reoviridae Infections - veterinary RNA, Viral - analysis SALMO SALAR Salmon viral diseases VIRUS NECROSE PANCREATIQUE VIRUS NECROSIS PANCREATICA viruses |
| title | Detection of infectious pancreatic necrosis virus (IPNV) RNA by hybridization with an oligonucleotide DNA probe |
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