Validation of the Point-EXACCT method in non-small cell lung carcinomas
K-ras point mutations are often detected in part of the lung carcinomas. For the validation of a highly sensitive and rapid assay for known point mutations, Point-EXACCT (Biochim Biophys Acta 1998; 1379:42-52), we analyzed 89 non-small cell lung carcinomas and compared the results with two sequencin...
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| Veröffentlicht in: | Clinical chemistry (Baltimore, Md.) Md.), 1998-07, Vol.44 (7), p.1404-1409 |
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| container_title | Clinical chemistry (Baltimore, Md.) |
| container_volume | 44 |
| creator | Somers, Veerle A. M. C Leimbach, Darcy A Theunissen, Paul H. M. H Murtagh, James J., Jr Holloway, Brian Ambergen, Anton W Thunnissen, Frederik B. J. M |
| description | K-ras point mutations are often detected in part of the lung carcinomas. For the validation of a highly sensitive and rapid assay for known point mutations, Point-EXACCT (Biochim Biophys Acta 1998; 1379:42-52), we analyzed 89 non-small cell lung carcinomas and compared the results with two sequencing methods. No point mutations were found with double-stranded sequencing. Single-stranded sequencing detected six patients positive for K-ras codon 12. When Point-EXACCT was used, K-ras codon 12 mutations were detected in 8 of 52 patients with squamous cell carcinomas, 10 of 29 patients with adenocarcinomas, and 3 of 8 patients with large cell carcinomas. The finding of K-ras mutations in squamous cell carcinomas is explained by the high sensitivity of the method. Therefore, Point-EXACCT may be applicable to detection of those alterations occurring at a low frequency among an excess of cells with wild-type DNA. |
| doi_str_mv | 10.1093/clinchem/44.7.1404 |
| format | Article |
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M. C ; Leimbach, Darcy A ; Theunissen, Paul H. M. H ; Murtagh, James J., Jr ; Holloway, Brian ; Ambergen, Anton W ; Thunnissen, Frederik B. J. M</creator><creatorcontrib>Somers, Veerle A. M. C ; Leimbach, Darcy A ; Theunissen, Paul H. M. H ; Murtagh, James J., Jr ; Holloway, Brian ; Ambergen, Anton W ; Thunnissen, Frederik B. J. M</creatorcontrib><description>K-ras point mutations are often detected in part of the lung carcinomas. For the validation of a highly sensitive and rapid assay for known point mutations, Point-EXACCT (Biochim Biophys Acta 1998; 1379:42-52), we analyzed 89 non-small cell lung carcinomas and compared the results with two sequencing methods. No point mutations were found with double-stranded sequencing. Single-stranded sequencing detected six patients positive for K-ras codon 12. When Point-EXACCT was used, K-ras codon 12 mutations were detected in 8 of 52 patients with squamous cell carcinomas, 10 of 29 patients with adenocarcinomas, and 3 of 8 patients with large cell carcinomas. The finding of K-ras mutations in squamous cell carcinomas is explained by the high sensitivity of the method. Therefore, Point-EXACCT may be applicable to detection of those alterations occurring at a low frequency among an excess of cells with wild-type DNA.</description><identifier>ISSN: 0009-9147</identifier><identifier>EISSN: 1530-8561</identifier><identifier>DOI: 10.1093/clinchem/44.7.1404</identifier><identifier>PMID: 9665416</identifier><identifier>CODEN: CLCHAU</identifier><language>eng</language><publisher>Washington, DC: Am Assoc Clin Chem</publisher><subject>Biological and medical sciences ; Carcinoma, Non-Small-Cell Lung - genetics ; DNA Mutational Analysis - methods ; DNA, Neoplasm - genetics ; Female ; Genes, ras ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Lung Neoplasms - genetics ; Male ; Medical sciences ; Nucleic Acid Amplification Techniques ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Point Mutation ; Reproducibility of Results ; Respiratory system ; Sensitivity and Specificity</subject><ispartof>Clinical chemistry (Baltimore, Md.), 1998-07, Vol.44 (7), p.1404-1409</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-d5ba9fb92eefd2ec38cbdcbeecc743c150824ffe2236c0f9a911e1ef7c4f7da33</citedby><cites>FETCH-LOGICAL-c402t-d5ba9fb92eefd2ec38cbdcbeecc743c150824ffe2236c0f9a911e1ef7c4f7da33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2314277$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9665416$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Somers, Veerle A. M. C</creatorcontrib><creatorcontrib>Leimbach, Darcy A</creatorcontrib><creatorcontrib>Theunissen, Paul H. M. H</creatorcontrib><creatorcontrib>Murtagh, James J., Jr</creatorcontrib><creatorcontrib>Holloway, Brian</creatorcontrib><creatorcontrib>Ambergen, Anton W</creatorcontrib><creatorcontrib>Thunnissen, Frederik B. J. M</creatorcontrib><title>Validation of the Point-EXACCT method in non-small cell lung carcinomas</title><title>Clinical chemistry (Baltimore, Md.)</title><addtitle>Clin Chem</addtitle><description>K-ras point mutations are often detected in part of the lung carcinomas. For the validation of a highly sensitive and rapid assay for known point mutations, Point-EXACCT (Biochim Biophys Acta 1998; 1379:42-52), we analyzed 89 non-small cell lung carcinomas and compared the results with two sequencing methods. No point mutations were found with double-stranded sequencing. Single-stranded sequencing detected six patients positive for K-ras codon 12. When Point-EXACCT was used, K-ras codon 12 mutations were detected in 8 of 52 patients with squamous cell carcinomas, 10 of 29 patients with adenocarcinomas, and 3 of 8 patients with large cell carcinomas. The finding of K-ras mutations in squamous cell carcinomas is explained by the high sensitivity of the method. Therefore, Point-EXACCT may be applicable to detection of those alterations occurring at a low frequency among an excess of cells with wild-type DNA.</description><subject>Biological and medical sciences</subject><subject>Carcinoma, Non-Small-Cell Lung - genetics</subject><subject>DNA Mutational Analysis - methods</subject><subject>DNA, Neoplasm - genetics</subject><subject>Female</subject><subject>Genes, ras</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Lung Neoplasms - genetics</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Point Mutation</subject><subject>Reproducibility of Results</subject><subject>Respiratory system</subject><subject>Sensitivity and Specificity</subject><issn>0009-9147</issn><issn>1530-8561</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLxEAMxwdRdH18AUHoQbx1nVc77VEWX7CgBxVvwzTNbEfaGe10Wfz2dh_qJSHkn3-SHyHnjE4ZLcU1tM5Dg921lFM1ZZLKPTJhmaBpkeVsn0wopWVaMqmOyHGMH2MpVZEfksMyzzPJ8gm5fzOtq83ggk-CTYYGk-fg_JDevt_MZi9Jh0MT6sT5xAefxs60bQI4hnbpFwmYHpwPnYmn5MCaNuLZLp-Q17vbl9lDOn-6f5zdzFOQlA9pnVWmtFXJEW3NEUQBVQ0VIoCSAlhGCy6tRc5FDtSWpmQMGVoF0qraCHFCrra-n334WmIcdOfi-iDjMSyjLjY_imIU8q0Q-hBjj1Z_9q4z_bdmVK_p6V96Wkqt9JreOHSxc19WHdZ_IztcY_9y1zcRTGt748HFPxkXTHKl_o9s3KJZuR71BtxoyvRqtfrf9wO21YfC</recordid><startdate>19980701</startdate><enddate>19980701</enddate><creator>Somers, Veerle A. M. 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M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Validation of the Point-EXACCT method in non-small cell lung carcinomas</atitle><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle><addtitle>Clin Chem</addtitle><date>1998-07-01</date><risdate>1998</risdate><volume>44</volume><issue>7</issue><spage>1404</spage><epage>1409</epage><pages>1404-1409</pages><issn>0009-9147</issn><eissn>1530-8561</eissn><coden>CLCHAU</coden><abstract>K-ras point mutations are often detected in part of the lung carcinomas. For the validation of a highly sensitive and rapid assay for known point mutations, Point-EXACCT (Biochim Biophys Acta 1998; 1379:42-52), we analyzed 89 non-small cell lung carcinomas and compared the results with two sequencing methods. No point mutations were found with double-stranded sequencing. Single-stranded sequencing detected six patients positive for K-ras codon 12. 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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Biological and medical sciences Carcinoma, Non-Small-Cell Lung - genetics DNA Mutational Analysis - methods DNA, Neoplasm - genetics Female Genes, ras Humans Investigative techniques, diagnostic techniques (general aspects) Lung Neoplasms - genetics Male Medical sciences Nucleic Acid Amplification Techniques Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Point Mutation Reproducibility of Results Respiratory system Sensitivity and Specificity |
| title | Validation of the Point-EXACCT method in non-small cell lung carcinomas |
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