Surface modification of superparamagnetic nanoparticles (Ferrofluid) studied with particle electrophoresis: Application to the specific targeting of cells

Colloidal aqueous suspension of superparamagnetic nanoparticles (9 nm in diameter) composed of maghemite (γ Fe2O3) and forming an ionic ferrofluid in aqueous solution are covalently coupled with lectins, enzymes or antibodies, using specific thiol chemistry. The surface charge modifications of nanop...

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Veröffentlicht in:	Electrophoresis 1998-06, Vol.19 (7), p.1220-1226
Hauptverfasser:	Sestier, Claude, Da-Silva, Maria Fatima, Sabolovic, Domagoj, Roger, Jackie, Pons, Jean Noël
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Line Cell separation Colloid Electrophoresis - methods Ferric Compounds - chemical synthesis Ferric Compounds - chemistry Ferrofluid Ferrosoferric Oxide Humans Hybridomas Iron - chemistry Laser-Doppler Flowmetry - methods Magnetics Mice Mice, Inbred C57BL Mice, Nude Oxides - chemistry Particle Size Superparamagnetic nanoparticles Surface Properties
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creator	Sestier, Claude Da-Silva, Maria Fatima Sabolovic, Domagoj Roger, Jackie Pons, Jean Noël
description	Colloidal aqueous suspension of superparamagnetic nanoparticles (9 nm in diameter) composed of maghemite (γ Fe2O3) and forming an ionic ferrofluid in aqueous solution are covalently coupled with lectins, enzymes or antibodies, using specific thiol chemistry. The surface charge modifications of nanoparticles, caused by ligand coupling, were monitored by measuring their electrophoretic mobilities using laser‐Doppler velocimetry. Particle electrophoretic mobility (PEM) changes are shown to correlate well with the amount of ligand fixed on the particles, as probed by its biological activity. The PEM method provides a useful tool to optimize ligand immobilization at the surface of nanoparticles, and may be advantageous when biological activity measurements are not convenient.
doi_str_mv	10.1002/elps.1150190725
format	Article
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source	MEDLINE; Wiley Online Library Journals Frontfile Complete
subjects	Animals Cell Line Cell separation Colloid Electrophoresis - methods Ferric Compounds - chemical synthesis Ferric Compounds - chemistry Ferrofluid Ferrosoferric Oxide Humans Hybridomas Iron - chemistry Laser-Doppler Flowmetry - methods Magnetics Mice Mice, Inbred C57BL Mice, Nude Oxides - chemistry Particle Size Superparamagnetic nanoparticles Surface Properties
title	Surface modification of superparamagnetic nanoparticles (Ferrofluid) studied with particle electrophoresis: Application to the specific targeting of cells
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