Protein separation using membrane-encapsulated soluble ligand conjugates
A new approach for isolating and recovering biological macromolecules using membrane-encapsulated soluble ligand conjugates was investigated. Membrane-encapsulated solid adsorbents have been successfully developed and employed in our laboratory to isolate and purify proteins and enzymes directly fro...
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| Veröffentlicht in: | Enzyme and microbial technology 1990-05, Vol.12 (5), p.349-354 |
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| container_title | Enzyme and microbial technology |
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| creator | Sakoda, Akiyoshi Nigam, Somesh C. Wang, Henry Y. |
| description | A new approach for isolating and recovering biological macromolecules using membrane-encapsulated soluble ligand conjugates was investigated. Membrane-encapsulated solid adsorbents have been successfully developed and employed in our laboratory to isolate and purify proteins and enzymes directly from culture broths. This new concept also makes it possible to use soluble ligand conjugates instead of solid adsorbents inside membrane capsules. In this work, model membrane-encapsulated soluble and insoluble ligands comprising Blue Dextran and Blue Sepharose entrapped within calcium alginate membranes were studied to compare adsorption characteristics such as capacities and rates. Experimental results suggest that membrane-encapsulated soluble ligands may be expected to result in higher overall adsorption capacity compared to membrane-encapsulated solid adsorbents with comparable adsorption rates. |
| doi_str_mv | 10.1016/0141-0229(90)90163-K |
| format | Article |
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Membrane-encapsulated solid adsorbents have been successfully developed and employed in our laboratory to isolate and purify proteins and enzymes directly from culture broths. This new concept also makes it possible to use soluble ligand conjugates instead of solid adsorbents inside membrane capsules. In this work, model membrane-encapsulated soluble and insoluble ligands comprising Blue Dextran and Blue Sepharose entrapped within calcium alginate membranes were studied to compare adsorption characteristics such as capacities and rates. Experimental results suggest that membrane-encapsulated soluble ligands may be expected to result in higher overall adsorption capacity compared to membrane-encapsulated solid adsorbents with comparable adsorption rates.</description><subject>Adsorption</subject><subject>affinity adsorption</subject><subject>affinity chromatography</subject><subject>Alginates</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Bioseparation</subject><subject>Biotechnology</subject><subject>Calcium</subject><subject>enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation methods</subject><subject>Glucuronic Acid</subject><subject>Hexuronic Acids</subject><subject>Humans</subject><subject>Ligands</subject><subject>membrane-encapsulated ligand</subject><subject>membranes</subject><subject>Membranes, Artificial</subject><subject>Proteins</subject><subject>Proteins - isolation & purification</subject><subject>separation techniques</subject><subject>Serum Albumin - isolation & purification</subject><subject>soluble ligand conjugate</subject><issn>0141-0229</issn><issn>1879-0909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMo67r6DxR6UfRQnTRt2lwEWfxCQQ96DvmYLpF-rEkr-O_NuovedC4D8z4zDA8hhxTOKVB-ATSnKWSZOBVwJuKEpQ9bZEqrUqQgQGyT6Q-yS_ZCeAOIgxwmZEIZ53lVTsnds-8HdF0ScKm8GlzfJWNw3SJpsdVedZhiZ9QyjI0a0Cahb0bdYNK4hepsYvrubVzEJOyTnVo1AQ82fUZeb65f5nfp49Pt_fzqMTUF50NaVYxnos7RGgCLwmChVW3KmgnLhcgUaFXqDLKi0pWxWBeaca2FUVCgpSWbkZP13aXv30cMg2xdMNg08dV-DLKCVTHxL0iLklHBigjma9D4PgSPtVx61yr_KSnIlWm50ihXGqUA-W1aPsS1o839Ubdof5fWamN-vMlVMKqpo0vjwi8mWFbxkkXucs1htPbh0MtgXHSO1nk0g7S9-_uRL4p6m1c</recordid><startdate>19900501</startdate><enddate>19900501</enddate><creator>Sakoda, Akiyoshi</creator><creator>Nigam, Somesh C.</creator><creator>Wang, Henry Y.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19900501</creationdate><title>Protein separation using membrane-encapsulated soluble ligand conjugates</title><author>Sakoda, Akiyoshi ; Nigam, Somesh C. ; Wang, Henry Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c566t-883629f4edc00de9ce5bafc7f39d6992a0ba7b20258b8cdef5b36bb9ca05ed173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Adsorption</topic><topic>affinity adsorption</topic><topic>affinity chromatography</topic><topic>Alginates</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Bioseparation</topic><topic>Biotechnology</topic><topic>Calcium</topic><topic>enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation methods</topic><topic>Glucuronic Acid</topic><topic>Hexuronic Acids</topic><topic>Humans</topic><topic>Ligands</topic><topic>membrane-encapsulated ligand</topic><topic>membranes</topic><topic>Membranes, Artificial</topic><topic>Proteins</topic><topic>Proteins - isolation & purification</topic><topic>separation techniques</topic><topic>Serum Albumin - isolation & purification</topic><topic>soluble ligand conjugate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakoda, Akiyoshi</creatorcontrib><creatorcontrib>Nigam, Somesh C.</creatorcontrib><creatorcontrib>Wang, Henry Y.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Enzyme and microbial technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakoda, Akiyoshi</au><au>Nigam, Somesh C.</au><au>Wang, Henry Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein separation using membrane-encapsulated soluble ligand conjugates</atitle><jtitle>Enzyme and microbial technology</jtitle><addtitle>Enzyme Microb Technol</addtitle><date>1990-05-01</date><risdate>1990</risdate><volume>12</volume><issue>5</issue><spage>349</spage><epage>354</epage><pages>349-354</pages><issn>0141-0229</issn><eissn>1879-0909</eissn><coden>EMTED2</coden><abstract>A new approach for isolating and recovering biological macromolecules using membrane-encapsulated soluble ligand conjugates was investigated. 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| identifier | ISSN: 0141-0229 |
| ispartof | Enzyme and microbial technology, 1990-05, Vol.12 (5), p.349-354 |
| issn | 0141-0229 1879-0909 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adsorption affinity adsorption affinity chromatography Alginates Analytical, structural and metabolic biochemistry Biological and medical sciences Bioseparation Biotechnology Calcium enzymes Fundamental and applied biological sciences. Psychology General aspects, investigation methods Glucuronic Acid Hexuronic Acids Humans Ligands membrane-encapsulated ligand membranes Membranes, Artificial Proteins Proteins - isolation & purification separation techniques Serum Albumin - isolation & purification soluble ligand conjugate |
| title | Protein separation using membrane-encapsulated soluble ligand conjugates |
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