Transplantation of stromal cells transduced with the human IL3 gene to stimulate hematopoiesis in human fetal bone grafts in non-obese, diabetic-severe combined immunodeficiency mice
The non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mouse is a convenient host for human hematopoietic tissues and cells. Human fetal bone fragments engrafted subcutaneously in NOD-SCID mice sustain human hematopoiesis for several months. MS5 murine bone marrow stromal cells were tran...
Gespeichert in:
| Veröffentlicht in: | Leukemia 1998-07, Vol.12 (7), p.1128-1135 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1135 |
|---|---|
| container_issue | 7 |
| container_start_page | 1128 |
| container_title | Leukemia |
| container_volume | 12 |
| creator | BROUARD, N CHAPEL, A NEILDEZ-NGUYEN, T. M. A GRANOTIER, C KHAZAAL, I PEAULT, B THIERRY, D |
| description | The non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mouse is a convenient host for human hematopoietic tissues and cells. Human fetal bone fragments engrafted subcutaneously in NOD-SCID mice sustain human hematopoiesis for several months. MS5 murine bone marrow stromal cells were transfected by electroporation with a plasmid containing the human interleukin-3 gene. As expected, stably transfected hu-IL3-MS5 cells supported human hematopoiesis in vitro more efficiently than MS5 cells. hu-IL3-MS5 cells were then injected intravenously into hu-NOD-SCID mice to test their ability to home to the mouse and/or human bone marrow, and to evaluate the role of hu-IL3 secretion on human hematopoiesis in vivo. hu-IL3 was detected in the mouse serum for up to an observation time of 8 weeks. hu-IL3-MS5 cells engrafted the bone marrow, spleen, liver and lungs of the mice but also the human bone graft. The presence of hu-IL3-MS5 cells in the human bone significantly stimulated local human hematopoiesis. This setting could be used to model the bone marrow homing of intravenously injected stromal cells or stromal cell precursors. The same experimental principle could also be applied in a therapeutic perspective to malignant human bone marrow hematopoiesis. |
| doi_str_mv | 10.1038/sj.leu.2401081 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79999198</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79999198</sourcerecordid><originalsourceid>FETCH-LOGICAL-c378t-1d287031942e32b1afb6807440070ca10a157485dbbd4a8ca316dc2f25ab03743</originalsourceid><addsrcrecordid>eNqFkT1vFDEQhi0ECkegpUOyRETFHv7e3RJFfEQ6KU1Sr2zvbM6ntX3Y3qD8sfw-fGSVgoZpXLzPvDOeF6H3lGwp4d2XfNjOsGyZIJR09AXaUNGqRkpJX6IN6bq2UT0Tr9GbnA-EnER1hs56pSQjZIMeb5IO-TjrUHRxMeA44VxS9HrGFuY543ICxsXCiH-7ssdlD3i_eB3w1Y7jOwiAS6w9zi-zLlUDr0s8RgfZZezCCk9QqqWJFb9Leip_pRBDEw1k-IxHpw0UZ5sM95AA2-iNC3Wo834JcYTJWQfBPmDvLLxFryY9Z3i3vufo9vu3m8ufze76x9Xl111jeduVho6sawmnvWDAmaF6MqojrRCEtMRqSjSVrejkaMwodGc1p2q0bGJSG8Jbwc_RpyffY4q_Fshl8C6f7qIDxCUPbV-L9t1_QVrvLesqFfz4D3iISwr1EwNTQqpecSkrtX2ibIo5J5iGY3Jep4eBkuGU-5APQ819WHOvDR9W28V4GJ_xNeiqX6y6zlbPUw3VuvyMMc64FIz_Af1guSo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2645696355</pqid></control><display><type>article</type><title>Transplantation of stromal cells transduced with the human IL3 gene to stimulate hematopoiesis in human fetal bone grafts in non-obese, diabetic-severe combined immunodeficiency mice</title><source>MEDLINE</source><source>Nature</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>SpringerLink Journals - AutoHoldings</source><creator>BROUARD, N ; CHAPEL, A ; NEILDEZ-NGUYEN, T. M. A ; GRANOTIER, C ; KHAZAAL, I ; PEAULT, B ; THIERRY, D</creator><creatorcontrib>BROUARD, N ; CHAPEL, A ; NEILDEZ-NGUYEN, T. M. A ; GRANOTIER, C ; KHAZAAL, I ; PEAULT, B ; THIERRY, D</creatorcontrib><description>The non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mouse is a convenient host for human hematopoietic tissues and cells. Human fetal bone fragments engrafted subcutaneously in NOD-SCID mice sustain human hematopoiesis for several months. MS5 murine bone marrow stromal cells were transfected by electroporation with a plasmid containing the human interleukin-3 gene. As expected, stably transfected hu-IL3-MS5 cells supported human hematopoiesis in vitro more efficiently than MS5 cells. hu-IL3-MS5 cells were then injected intravenously into hu-NOD-SCID mice to test their ability to home to the mouse and/or human bone marrow, and to evaluate the role of hu-IL3 secretion on human hematopoiesis in vivo. hu-IL3 was detected in the mouse serum for up to an observation time of 8 weeks. hu-IL3-MS5 cells engrafted the bone marrow, spleen, liver and lungs of the mice but also the human bone graft. The presence of hu-IL3-MS5 cells in the human bone significantly stimulated local human hematopoiesis. This setting could be used to model the bone marrow homing of intravenously injected stromal cells or stromal cell precursors. The same experimental principle could also be applied in a therapeutic perspective to malignant human bone marrow hematopoiesis.</description><identifier>ISSN: 0887-6924</identifier><identifier>EISSN: 1476-5551</identifier><identifier>DOI: 10.1038/sj.leu.2401081</identifier><identifier>PMID: 9665200</identifier><identifier>CODEN: LEUKED</identifier><language>eng</language><publisher>London: Nature Publishing</publisher><subject>Animals ; Biological and medical sciences ; Bone and Bones - embryology ; Bone and Bones - metabolism ; Bone marrow ; Bone Marrow Cells - metabolism ; Bone Marrow Cells - physiology ; Bone Marrow Transplantation ; Bone Transplantation ; Cell physiology ; Cells, Cultured ; Diabetes ; Diabetes mellitus ; Electroporation ; Fetal Tissue Transplantation ; Fetuses ; Fundamental and applied biological sciences. Psychology ; Grafting ; Hematopoiesis ; Hematopoiesis - physiology ; Hemopoiesis ; Homing behavior ; Humans ; Immune system ; In vivo methods and tests ; Injections, Intravenous ; Interleukin 3 ; Interleukin-3 - biosynthesis ; Interleukin-3 - genetics ; Interleukin-3 - physiology ; Male ; Membrane and intracellular transports ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Molecular and cellular biology ; Spleen ; Stromal cells ; Stromal Cells - metabolism ; Stromal Cells - physiology ; Stromal Cells - transplantation ; Substitute bone ; Transduction, Genetic ; Transplantation</subject><ispartof>Leukemia, 1998-07, Vol.12 (7), p.1128-1135</ispartof><rights>1998 INIST-CNRS</rights><rights>Macmillan Publishers Limited 1998.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-1d287031942e32b1afb6807440070ca10a157485dbbd4a8ca316dc2f25ab03743</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2323542$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9665200$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BROUARD, N</creatorcontrib><creatorcontrib>CHAPEL, A</creatorcontrib><creatorcontrib>NEILDEZ-NGUYEN, T. M. A</creatorcontrib><creatorcontrib>GRANOTIER, C</creatorcontrib><creatorcontrib>KHAZAAL, I</creatorcontrib><creatorcontrib>PEAULT, B</creatorcontrib><creatorcontrib>THIERRY, D</creatorcontrib><title>Transplantation of stromal cells transduced with the human IL3 gene to stimulate hematopoiesis in human fetal bone grafts in non-obese, diabetic-severe combined immunodeficiency mice</title><title>Leukemia</title><addtitle>Leukemia</addtitle><description>The non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mouse is a convenient host for human hematopoietic tissues and cells. Human fetal bone fragments engrafted subcutaneously in NOD-SCID mice sustain human hematopoiesis for several months. MS5 murine bone marrow stromal cells were transfected by electroporation with a plasmid containing the human interleukin-3 gene. As expected, stably transfected hu-IL3-MS5 cells supported human hematopoiesis in vitro more efficiently than MS5 cells. hu-IL3-MS5 cells were then injected intravenously into hu-NOD-SCID mice to test their ability to home to the mouse and/or human bone marrow, and to evaluate the role of hu-IL3 secretion on human hematopoiesis in vivo. hu-IL3 was detected in the mouse serum for up to an observation time of 8 weeks. hu-IL3-MS5 cells engrafted the bone marrow, spleen, liver and lungs of the mice but also the human bone graft. The presence of hu-IL3-MS5 cells in the human bone significantly stimulated local human hematopoiesis. This setting could be used to model the bone marrow homing of intravenously injected stromal cells or stromal cell precursors. The same experimental principle could also be applied in a therapeutic perspective to malignant human bone marrow hematopoiesis.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone and Bones - embryology</subject><subject>Bone and Bones - metabolism</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Bone Marrow Cells - physiology</subject><subject>Bone Marrow Transplantation</subject><subject>Bone Transplantation</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Electroporation</subject><subject>Fetal Tissue Transplantation</subject><subject>Fetuses</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Grafting</subject><subject>Hematopoiesis</subject><subject>Hematopoiesis - physiology</subject><subject>Hemopoiesis</subject><subject>Homing behavior</subject><subject>Humans</subject><subject>Immune system</subject><subject>In vivo methods and tests</subject><subject>Injections, Intravenous</subject><subject>Interleukin 3</subject><subject>Interleukin-3 - biosynthesis</subject><subject>Interleukin-3 - genetics</subject><subject>Interleukin-3 - physiology</subject><subject>Male</subject><subject>Membrane and intracellular transports</subject><subject>Mice</subject><subject>Mice, Inbred NOD</subject><subject>Mice, SCID</subject><subject>Molecular and cellular biology</subject><subject>Spleen</subject><subject>Stromal cells</subject><subject>Stromal Cells - metabolism</subject><subject>Stromal Cells - physiology</subject><subject>Stromal Cells - transplantation</subject><subject>Substitute bone</subject><subject>Transduction, Genetic</subject><subject>Transplantation</subject><issn>0887-6924</issn><issn>1476-5551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT1vFDEQhi0ECkegpUOyRETFHv7e3RJFfEQ6KU1Sr2zvbM6ntX3Y3qD8sfw-fGSVgoZpXLzPvDOeF6H3lGwp4d2XfNjOsGyZIJR09AXaUNGqRkpJX6IN6bq2UT0Tr9GbnA-EnER1hs56pSQjZIMeb5IO-TjrUHRxMeA44VxS9HrGFuY543ICxsXCiH-7ssdlD3i_eB3w1Y7jOwiAS6w9zi-zLlUDr0s8RgfZZezCCk9QqqWJFb9Leip_pRBDEw1k-IxHpw0UZ5sM95AA2-iNC3Wo834JcYTJWQfBPmDvLLxFryY9Z3i3vufo9vu3m8ufze76x9Xl111jeduVho6sawmnvWDAmaF6MqojrRCEtMRqSjSVrejkaMwodGc1p2q0bGJSG8Jbwc_RpyffY4q_Fshl8C6f7qIDxCUPbV-L9t1_QVrvLesqFfz4D3iISwr1EwNTQqpecSkrtX2ibIo5J5iGY3Jep4eBkuGU-5APQ819WHOvDR9W28V4GJ_xNeiqX6y6zlbPUw3VuvyMMc64FIz_Af1guSo</recordid><startdate>19980701</startdate><enddate>19980701</enddate><creator>BROUARD, N</creator><creator>CHAPEL, A</creator><creator>NEILDEZ-NGUYEN, T. M. A</creator><creator>GRANOTIER, C</creator><creator>KHAZAAL, I</creator><creator>PEAULT, B</creator><creator>THIERRY, D</creator><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>19980701</creationdate><title>Transplantation of stromal cells transduced with the human IL3 gene to stimulate hematopoiesis in human fetal bone grafts in non-obese, diabetic-severe combined immunodeficiency mice</title><author>BROUARD, N ; CHAPEL, A ; NEILDEZ-NGUYEN, T. M. A ; GRANOTIER, C ; KHAZAAL, I ; PEAULT, B ; THIERRY, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-1d287031942e32b1afb6807440070ca10a157485dbbd4a8ca316dc2f25ab03743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone and Bones - embryology</topic><topic>Bone and Bones - metabolism</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Bone Marrow Cells - physiology</topic><topic>Bone Marrow Transplantation</topic><topic>Bone Transplantation</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Electroporation</topic><topic>Fetal Tissue Transplantation</topic><topic>Fetuses</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Grafting</topic><topic>Hematopoiesis</topic><topic>Hematopoiesis - physiology</topic><topic>Hemopoiesis</topic><topic>Homing behavior</topic><topic>Humans</topic><topic>Immune system</topic><topic>In vivo methods and tests</topic><topic>Injections, Intravenous</topic><topic>Interleukin 3</topic><topic>Interleukin-3 - biosynthesis</topic><topic>Interleukin-3 - genetics</topic><topic>Interleukin-3 - physiology</topic><topic>Male</topic><topic>Membrane and intracellular transports</topic><topic>Mice</topic><topic>Mice, Inbred NOD</topic><topic>Mice, SCID</topic><topic>Molecular and cellular biology</topic><topic>Spleen</topic><topic>Stromal cells</topic><topic>Stromal Cells - metabolism</topic><topic>Stromal Cells - physiology</topic><topic>Stromal Cells - transplantation</topic><topic>Substitute bone</topic><topic>Transduction, Genetic</topic><topic>Transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BROUARD, N</creatorcontrib><creatorcontrib>CHAPEL, A</creatorcontrib><creatorcontrib>NEILDEZ-NGUYEN, T. M. A</creatorcontrib><creatorcontrib>GRANOTIER, C</creatorcontrib><creatorcontrib>KHAZAAL, I</creatorcontrib><creatorcontrib>PEAULT, B</creatorcontrib><creatorcontrib>THIERRY, D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Leukemia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BROUARD, N</au><au>CHAPEL, A</au><au>NEILDEZ-NGUYEN, T. M. A</au><au>GRANOTIER, C</au><au>KHAZAAL, I</au><au>PEAULT, B</au><au>THIERRY, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transplantation of stromal cells transduced with the human IL3 gene to stimulate hematopoiesis in human fetal bone grafts in non-obese, diabetic-severe combined immunodeficiency mice</atitle><jtitle>Leukemia</jtitle><addtitle>Leukemia</addtitle><date>1998-07-01</date><risdate>1998</risdate><volume>12</volume><issue>7</issue><spage>1128</spage><epage>1135</epage><pages>1128-1135</pages><issn>0887-6924</issn><eissn>1476-5551</eissn><coden>LEUKED</coden><abstract>The non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mouse is a convenient host for human hematopoietic tissues and cells. Human fetal bone fragments engrafted subcutaneously in NOD-SCID mice sustain human hematopoiesis for several months. MS5 murine bone marrow stromal cells were transfected by electroporation with a plasmid containing the human interleukin-3 gene. As expected, stably transfected hu-IL3-MS5 cells supported human hematopoiesis in vitro more efficiently than MS5 cells. hu-IL3-MS5 cells were then injected intravenously into hu-NOD-SCID mice to test their ability to home to the mouse and/or human bone marrow, and to evaluate the role of hu-IL3 secretion on human hematopoiesis in vivo. hu-IL3 was detected in the mouse serum for up to an observation time of 8 weeks. hu-IL3-MS5 cells engrafted the bone marrow, spleen, liver and lungs of the mice but also the human bone graft. The presence of hu-IL3-MS5 cells in the human bone significantly stimulated local human hematopoiesis. This setting could be used to model the bone marrow homing of intravenously injected stromal cells or stromal cell precursors. The same experimental principle could also be applied in a therapeutic perspective to malignant human bone marrow hematopoiesis.</abstract><cop>London</cop><pub>Nature Publishing</pub><pmid>9665200</pmid><doi>10.1038/sj.leu.2401081</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0887-6924 |
| ispartof | Leukemia, 1998-07, Vol.12 (7), p.1128-1135 |
| issn | 0887-6924 1476-5551 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79999198 |
| source | MEDLINE; Nature; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biological and medical sciences Bone and Bones - embryology Bone and Bones - metabolism Bone marrow Bone Marrow Cells - metabolism Bone Marrow Cells - physiology Bone Marrow Transplantation Bone Transplantation Cell physiology Cells, Cultured Diabetes Diabetes mellitus Electroporation Fetal Tissue Transplantation Fetuses Fundamental and applied biological sciences. Psychology Grafting Hematopoiesis Hematopoiesis - physiology Hemopoiesis Homing behavior Humans Immune system In vivo methods and tests Injections, Intravenous Interleukin 3 Interleukin-3 - biosynthesis Interleukin-3 - genetics Interleukin-3 - physiology Male Membrane and intracellular transports Mice Mice, Inbred NOD Mice, SCID Molecular and cellular biology Spleen Stromal cells Stromal Cells - metabolism Stromal Cells - physiology Stromal Cells - transplantation Substitute bone Transduction, Genetic Transplantation |
| title | Transplantation of stromal cells transduced with the human IL3 gene to stimulate hematopoiesis in human fetal bone grafts in non-obese, diabetic-severe combined immunodeficiency mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T21%3A28%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Transplantation%20of%20stromal%20cells%20transduced%20with%20the%20human%20IL3%20gene%20to%20stimulate%20hematopoiesis%20in%20human%20fetal%20bone%20grafts%20in%20non-obese,%20diabetic-severe%20combined%20immunodeficiency%20mice&rft.jtitle=Leukemia&rft.au=BROUARD,%20N&rft.date=1998-07-01&rft.volume=12&rft.issue=7&rft.spage=1128&rft.epage=1135&rft.pages=1128-1135&rft.issn=0887-6924&rft.eissn=1476-5551&rft.coden=LEUKED&rft_id=info:doi/10.1038/sj.leu.2401081&rft_dat=%3Cproquest_cross%3E79999198%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2645696355&rft_id=info:pmid/9665200&rfr_iscdi=true |