Calcium involvement in the muscarinic response of the gastric parietal cell
The influence of extracellular Ca 2+ on the mediation of carbachol stimulation in isolated rabbit gastric parietal cells was studied. Removing Ca 2+ from extracellular medium caused a 42% decrease of the aminopyrine accumulation due to carbachol with the same EC 50 value (∼ 5 μM). A short time deple...
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| Veröffentlicht in: | Cellular signalling 1990, Vol.2 (2), p.177-186 |
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| creator | Leonard, A. Guillon, G. Choquet, A. Bali, J.P. |
| description | The influence of extracellular Ca
2+ on the mediation of carbachol stimulation in isolated rabbit gastric parietal cells was studied. Removing Ca
2+ from extracellular medium caused a 42% decrease of the aminopyrine accumulation due to carbachol with the same EC
50 value (∼ 5 μM). A short time depletion in extracellular calcium suppressed the carbachol-dependent Ca
2+ influx without affecting Ca
2+ release from internal stores (fura-2 measurements). Similarly, the production of inositol phosphates under cholinergic stimulation was reduced by 29%. A rapid increase in Ins(1, 4, 5)P
3 was obtained 5 s after carbachol stimulation, and this increase was not changed in Ca
2+-dependent medium. In contrast, a 20 min incubation with carbachol caused a 50% reduction in both basal and carbachol-stimulated inositol phosphate accumulations. In conclusion, phospholipase C activation, intracellular Ca
2+ release and aminopyrine accumulation were sequentially observed following carbachol stimulation of the isolated gastric parietal cell and extracellular calcium contributed to sustain this acid secretory response. |
| doi_str_mv | 10.1016/0898-6568(90)90021-2 |
| format | Article |
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2+ on the mediation of carbachol stimulation in isolated rabbit gastric parietal cells was studied. Removing Ca
2+ from extracellular medium caused a 42% decrease of the aminopyrine accumulation due to carbachol with the same EC
50 value (∼ 5 μM). A short time depletion in extracellular calcium suppressed the carbachol-dependent Ca
2+ influx without affecting Ca
2+ release from internal stores (fura-2 measurements). Similarly, the production of inositol phosphates under cholinergic stimulation was reduced by 29%. A rapid increase in Ins(1, 4, 5)P
3 was obtained 5 s after carbachol stimulation, and this increase was not changed in Ca
2+-dependent medium. In contrast, a 20 min incubation with carbachol caused a 50% reduction in both basal and carbachol-stimulated inositol phosphate accumulations. In conclusion, phospholipase C activation, intracellular Ca
2+ release and aminopyrine accumulation were sequentially observed following carbachol stimulation of the isolated gastric parietal cell and extracellular calcium contributed to sustain this acid secretory response.</description><identifier>ISSN: 0898-6568</identifier><identifier>EISSN: 1873-3913</identifier><identifier>DOI: 10.1016/0898-6568(90)90021-2</identifier><identifier>PMID: 2400633</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>acid secretion ; Aminopyrine - metabolism ; Animals ; Calcium ; Calcium - metabolism ; Carbachol - pharmacology ; Cells, Cultured ; Chromatography, High Pressure Liquid ; Dose-Response Relationship, Drug ; fura-2 ; inositol phosphates ; Inositol Phosphates - metabolism ; Kinetics ; muscarinic receptor ; parietal cell ; Parietal Cells, Gastric - metabolism ; rabbit ; Rabbits ; Receptors, Muscarinic - metabolism</subject><ispartof>Cellular signalling, 1990, Vol.2 (2), p.177-186</ispartof><rights>1990</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-fdc03aa48f077878b04b8938b8671596b1b51cf93d9f576f17c5d68379c08ec13</citedby><cites>FETCH-LOGICAL-c357t-fdc03aa48f077878b04b8938b8671596b1b51cf93d9f576f17c5d68379c08ec13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0898-6568(90)90021-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,4010,27904,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2400633$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leonard, A.</creatorcontrib><creatorcontrib>Guillon, G.</creatorcontrib><creatorcontrib>Choquet, A.</creatorcontrib><creatorcontrib>Bali, J.P.</creatorcontrib><title>Calcium involvement in the muscarinic response of the gastric parietal cell</title><title>Cellular signalling</title><addtitle>Cell Signal</addtitle><description>The influence of extracellular Ca
2+ on the mediation of carbachol stimulation in isolated rabbit gastric parietal cells was studied. Removing Ca
2+ from extracellular medium caused a 42% decrease of the aminopyrine accumulation due to carbachol with the same EC
50 value (∼ 5 μM). A short time depletion in extracellular calcium suppressed the carbachol-dependent Ca
2+ influx without affecting Ca
2+ release from internal stores (fura-2 measurements). Similarly, the production of inositol phosphates under cholinergic stimulation was reduced by 29%. A rapid increase in Ins(1, 4, 5)P
3 was obtained 5 s after carbachol stimulation, and this increase was not changed in Ca
2+-dependent medium. In contrast, a 20 min incubation with carbachol caused a 50% reduction in both basal and carbachol-stimulated inositol phosphate accumulations. In conclusion, phospholipase C activation, intracellular Ca
2+ release and aminopyrine accumulation were sequentially observed following carbachol stimulation of the isolated gastric parietal cell and extracellular calcium contributed to sustain this acid secretory response.</description><subject>acid secretion</subject><subject>Aminopyrine - metabolism</subject><subject>Animals</subject><subject>Calcium</subject><subject>Calcium - metabolism</subject><subject>Carbachol - pharmacology</subject><subject>Cells, Cultured</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Dose-Response Relationship, Drug</subject><subject>fura-2</subject><subject>inositol phosphates</subject><subject>Inositol Phosphates - metabolism</subject><subject>Kinetics</subject><subject>muscarinic receptor</subject><subject>parietal cell</subject><subject>Parietal Cells, Gastric - metabolism</subject><subject>rabbit</subject><subject>Rabbits</subject><subject>Receptors, Muscarinic - metabolism</subject><issn>0898-6568</issn><issn>1873-3913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMlOwzAQhi0EKqXwBiDlhOAQGMeJlwsSqthEJS5wthxnAkbZsJNKvD1JW_XIaUbz_7N9hJxTuKFA-S1IJWOecXml4FoBJDRODsicSsFipig7JPO95ZichPANQDPgyYzMkhSAMzYnr0tTWTfUkWvWbbXGGpt-zKP-C6N6CNZ41zgbeQxd2wSM2nIjfZrQ-7HejTr2poosVtUpOSpNFfBsFxfk4_Hhffkcr96eXpb3q9iyTPRxWVhgxqSyBCGkkDmkuVRM5pILmime0zyjtlSsUGUmeEmFzQoumVAWJFrKFuRyO7fz7c-Aode1C9MBpsF2CFoopSQwPhrTrdH6NgSPpe68q43_1RT0xFBPgPQESCvQG4Y6GdsudvOHvMZi37SDNup3Wx3HJ9cOvQ7WYWOxcB5tr4vW_b_gD0jXgCw</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Leonard, A.</creator><creator>Guillon, G.</creator><creator>Choquet, A.</creator><creator>Bali, J.P.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>Calcium involvement in the muscarinic response of the gastric parietal cell</title><author>Leonard, A. ; Guillon, G. ; Choquet, A. ; Bali, J.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-fdc03aa48f077878b04b8938b8671596b1b51cf93d9f576f17c5d68379c08ec13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>acid secretion</topic><topic>Aminopyrine - metabolism</topic><topic>Animals</topic><topic>Calcium</topic><topic>Calcium - metabolism</topic><topic>Carbachol - pharmacology</topic><topic>Cells, Cultured</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Dose-Response Relationship, Drug</topic><topic>fura-2</topic><topic>inositol phosphates</topic><topic>Inositol Phosphates - metabolism</topic><topic>Kinetics</topic><topic>muscarinic receptor</topic><topic>parietal cell</topic><topic>Parietal Cells, Gastric - metabolism</topic><topic>rabbit</topic><topic>Rabbits</topic><topic>Receptors, Muscarinic - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leonard, A.</creatorcontrib><creatorcontrib>Guillon, G.</creatorcontrib><creatorcontrib>Choquet, A.</creatorcontrib><creatorcontrib>Bali, J.P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular signalling</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leonard, A.</au><au>Guillon, G.</au><au>Choquet, A.</au><au>Bali, J.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calcium involvement in the muscarinic response of the gastric parietal cell</atitle><jtitle>Cellular signalling</jtitle><addtitle>Cell Signal</addtitle><date>1990</date><risdate>1990</risdate><volume>2</volume><issue>2</issue><spage>177</spage><epage>186</epage><pages>177-186</pages><issn>0898-6568</issn><eissn>1873-3913</eissn><abstract>The influence of extracellular Ca
2+ on the mediation of carbachol stimulation in isolated rabbit gastric parietal cells was studied. Removing Ca
2+ from extracellular medium caused a 42% decrease of the aminopyrine accumulation due to carbachol with the same EC
50 value (∼ 5 μM). A short time depletion in extracellular calcium suppressed the carbachol-dependent Ca
2+ influx without affecting Ca
2+ release from internal stores (fura-2 measurements). Similarly, the production of inositol phosphates under cholinergic stimulation was reduced by 29%. A rapid increase in Ins(1, 4, 5)P
3 was obtained 5 s after carbachol stimulation, and this increase was not changed in Ca
2+-dependent medium. In contrast, a 20 min incubation with carbachol caused a 50% reduction in both basal and carbachol-stimulated inositol phosphate accumulations. In conclusion, phospholipase C activation, intracellular Ca
2+ release and aminopyrine accumulation were sequentially observed following carbachol stimulation of the isolated gastric parietal cell and extracellular calcium contributed to sustain this acid secretory response.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>2400633</pmid><doi>10.1016/0898-6568(90)90021-2</doi><tpages>10</tpages></addata></record> |
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| ispartof | Cellular signalling, 1990, Vol.2 (2), p.177-186 |
| issn | 0898-6568 1873-3913 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | acid secretion Aminopyrine - metabolism Animals Calcium Calcium - metabolism Carbachol - pharmacology Cells, Cultured Chromatography, High Pressure Liquid Dose-Response Relationship, Drug fura-2 inositol phosphates Inositol Phosphates - metabolism Kinetics muscarinic receptor parietal cell Parietal Cells, Gastric - metabolism rabbit Rabbits Receptors, Muscarinic - metabolism |
| title | Calcium involvement in the muscarinic response of the gastric parietal cell |
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