Effect of Manganese Deficiency on Insulin Binding, Glucose Transport and Metabolism in Rat Adipocytes
The effect of manganese deficiency on insulin binding, glucose transport and metabolism in isolated adipose cells from Sprague-Dawley rats was investigated. Offspring from Mn-sufficient female rats fed 45 µg Mn/g diet (control) and from Mn-deficient (Mn-) female rats fed 1 µg Mn/g diet were used in...
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| Veröffentlicht in: | The Journal of nutrition 1990-09, Vol.120 (9), p.1075-1079 |
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| creator | Baly, Deborah L. Schneiderman, Joanna S. Garcia-Welsh, Adrienne L. |
| description | The effect of manganese deficiency on insulin binding, glucose transport and metabolism in isolated adipose cells from Sprague-Dawley rats was investigated. Offspring from Mn-sufficient female rats fed 45 µg Mn/g diet (control) and from Mn-deficient (Mn-) female rats fed 1 µg Mn/g diet were used in these studies. Both basal and insulin-stimulated 3-O-methylglucose transport in isolated adipose cells was significantly lower in Mn- rats, averaging 40% and 50% of control values, respectively. Kinetic analysis of glucose transport demonstrated a lower maximal transport velocity (Vmax) for glucose in adipose cells from Mn- rats compared to controls. No differences in the Km for glucose uptake were observed between the two groups. Insulin-stimulated glucose oxidation to CO2 and conversion to triglycerides was lower in isolated adipose cells from Mn- rats compared to controls. Mn- animals had fewer insulin receptors per cell compared to controls, although no differences in insulin receptor affinity were observed between the two groups. These data suggest that Mn deficiency affects glucose transport and metabolism in the adipose cell. The apparent defect lies distal to the insulin receptor and probably reflects a decreased number of glucose transporters in adipose tissue of Mn- rats. |
| doi_str_mv | 10.1093/jn/120.9.1075 |
| format | Article |
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Offspring from Mn-sufficient female rats fed 45 µg Mn/g diet (control) and from Mn-deficient (Mn-) female rats fed 1 µg Mn/g diet were used in these studies. Both basal and insulin-stimulated 3-O-methylglucose transport in isolated adipose cells was significantly lower in Mn- rats, averaging 40% and 50% of control values, respectively. Kinetic analysis of glucose transport demonstrated a lower maximal transport velocity (Vmax) for glucose in adipose cells from Mn- rats compared to controls. No differences in the Km for glucose uptake were observed between the two groups. Insulin-stimulated glucose oxidation to CO2 and conversion to triglycerides was lower in isolated adipose cells from Mn- rats compared to controls. Mn- animals had fewer insulin receptors per cell compared to controls, although no differences in insulin receptor affinity were observed between the two groups. These data suggest that Mn deficiency affects glucose transport and metabolism in the adipose cell. The apparent defect lies distal to the insulin receptor and probably reflects a decreased number of glucose transporters in adipose tissue of Mn- rats.</description><identifier>ISSN: 0022-3166</identifier><identifier>EISSN: 1541-6100</identifier><identifier>DOI: 10.1093/jn/120.9.1075</identifier><identifier>PMID: 2204694</identifier><identifier>CODEN: JONUAI</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>adipocytes ; Adipose Tissue - cytology ; Adipose Tissue - metabolism ; Animals ; Biological and medical sciences ; Biological Transport, Active ; Carbohydrates ; carence minerale ; cells ; Cells, Cultured ; cellule ; celulas ; deficiencia de minerales ; diet ; dieta ; Female ; Fundamental and applied biological sciences. Psychology ; glucosa ; glucose ; Glucose - metabolism ; glucose transport ; Insulin - metabolism ; insulin receptor ; Kinetics ; manganese ; Manganese - deficiency ; manganeso ; metabolism ; metabolisme ; metabolismo ; Metabolisms and neurohumoral controls ; mineral deficiencies ; nutrient transport ; Pregnancy ; rat ; rata ; Rats ; Rats, Inbred Strains ; regime alimentaire ; transport des substances nutritives ; transporte de nutrientes ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>The Journal of nutrition, 1990-09, Vol.120 (9), p.1075-1079</ispartof><rights>1990 American Society for Nutrition.</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-e71b88a11b720e7cfd3f75d2121e351d8d7d543bd6522d8692d80afc8e60779f3</citedby><cites>FETCH-LOGICAL-c494t-e71b88a11b720e7cfd3f75d2121e351d8d7d543bd6522d8692d80afc8e60779f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19398013$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2204694$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baly, Deborah L.</creatorcontrib><creatorcontrib>Schneiderman, Joanna S.</creatorcontrib><creatorcontrib>Garcia-Welsh, Adrienne L.</creatorcontrib><creatorcontrib>The State University of New Jersey, New Brunswick, NJ</creatorcontrib><title>Effect of Manganese Deficiency on Insulin Binding, Glucose Transport and Metabolism in Rat Adipocytes</title><title>The Journal of nutrition</title><addtitle>J Nutr</addtitle><description>The effect of manganese deficiency on insulin binding, glucose transport and metabolism in isolated adipose cells from Sprague-Dawley rats was investigated. Offspring from Mn-sufficient female rats fed 45 µg Mn/g diet (control) and from Mn-deficient (Mn-) female rats fed 1 µg Mn/g diet were used in these studies. Both basal and insulin-stimulated 3-O-methylglucose transport in isolated adipose cells was significantly lower in Mn- rats, averaging 40% and 50% of control values, respectively. Kinetic analysis of glucose transport demonstrated a lower maximal transport velocity (Vmax) for glucose in adipose cells from Mn- rats compared to controls. No differences in the Km for glucose uptake were observed between the two groups. Insulin-stimulated glucose oxidation to CO2 and conversion to triglycerides was lower in isolated adipose cells from Mn- rats compared to controls. Mn- animals had fewer insulin receptors per cell compared to controls, although no differences in insulin receptor affinity were observed between the two groups. These data suggest that Mn deficiency affects glucose transport and metabolism in the adipose cell. The apparent defect lies distal to the insulin receptor and probably reflects a decreased number of glucose transporters in adipose tissue of Mn- rats.</description><subject>adipocytes</subject><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Transport, Active</subject><subject>Carbohydrates</subject><subject>carence minerale</subject><subject>cells</subject><subject>Cells, Cultured</subject><subject>cellule</subject><subject>celulas</subject><subject>deficiencia de minerales</subject><subject>diet</subject><subject>dieta</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glucosa</subject><subject>glucose</subject><subject>Glucose - metabolism</subject><subject>glucose transport</subject><subject>Insulin - metabolism</subject><subject>insulin receptor</subject><subject>Kinetics</subject><subject>manganese</subject><subject>Manganese - deficiency</subject><subject>manganeso</subject><subject>metabolism</subject><subject>metabolisme</subject><subject>metabolismo</subject><subject>Metabolisms and neurohumoral controls</subject><subject>mineral deficiencies</subject><subject>nutrient transport</subject><subject>Pregnancy</subject><subject>rat</subject><subject>rata</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>regime alimentaire</subject><subject>transport des substances nutritives</subject><subject>transporte de nutrientes</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0022-3166</issn><issn>1541-6100</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1vFCEYh4nR1G316FHDxZ46LV8zDMd-26SNibZnwsDLhs0srDBjsv-9bHejJy8Q8j75hff5IfSJknNKFL9YxQvKyLmqL9m-QQvaCtp0lJC3aEEIYw2nXfceHZeyIoRQofojdMQYEZ0SCwS33oOdcPL4ycSliVAA34APNkC0W5wifohlHkPEVyG6EJdn-H6cbarYczaxbFKesIkOP8FkhjSGssYV_mEmfOnCJtntBOUDeufNWODj4T5BL3e3z9ffmsfv9w_Xl4-NFUpMDUg69L2hdJCMgLTecS9bxyijwFvqeiddK_jgupYx13eqHsR420NHpFSen6DTfe4mp18zlEmvQ7EwjnWvNBctlVKd7EQFmz1ocyolg9ebHNYmbzUleqdVr6KuWrXSO62V_3wInoc1uL_0wWOdfz3MTbFm9NWMDeVfqOKqJ5RX7sue8yZps8yVeflJldoVw7lgFZB7AKqm3wGyLq9NgAu59qRdCv_54h_pmZsg</recordid><startdate>19900901</startdate><enddate>19900901</enddate><creator>Baly, Deborah L.</creator><creator>Schneiderman, Joanna S.</creator><creator>Garcia-Welsh, Adrienne L.</creator><general>Elsevier Inc</general><general>American Society for Nutritional Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900901</creationdate><title>Effect of Manganese Deficiency on Insulin Binding, Glucose Transport and Metabolism in Rat Adipocytes</title><author>Baly, Deborah L. ; Schneiderman, Joanna S. ; Garcia-Welsh, Adrienne L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-e71b88a11b720e7cfd3f75d2121e351d8d7d543bd6522d8692d80afc8e60779f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>adipocytes</topic><topic>Adipose Tissue - cytology</topic><topic>Adipose Tissue - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Transport, Active</topic><topic>Carbohydrates</topic><topic>carence minerale</topic><topic>cells</topic><topic>Cells, Cultured</topic><topic>cellule</topic><topic>celulas</topic><topic>deficiencia de minerales</topic><topic>diet</topic><topic>dieta</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glucosa</topic><topic>glucose</topic><topic>Glucose - metabolism</topic><topic>glucose transport</topic><topic>Insulin - metabolism</topic><topic>insulin receptor</topic><topic>Kinetics</topic><topic>manganese</topic><topic>Manganese - deficiency</topic><topic>manganeso</topic><topic>metabolism</topic><topic>metabolisme</topic><topic>metabolismo</topic><topic>Metabolisms and neurohumoral controls</topic><topic>mineral deficiencies</topic><topic>nutrient transport</topic><topic>Pregnancy</topic><topic>rat</topic><topic>rata</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>regime alimentaire</topic><topic>transport des substances nutritives</topic><topic>transporte de nutrientes</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baly, Deborah L.</creatorcontrib><creatorcontrib>Schneiderman, Joanna S.</creatorcontrib><creatorcontrib>Garcia-Welsh, Adrienne L.</creatorcontrib><creatorcontrib>The State University of New Jersey, New Brunswick, NJ</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baly, Deborah L.</au><au>Schneiderman, Joanna S.</au><au>Garcia-Welsh, Adrienne L.</au><aucorp>The State University of New Jersey, New Brunswick, NJ</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Manganese Deficiency on Insulin Binding, Glucose Transport and Metabolism in Rat Adipocytes</atitle><jtitle>The Journal of nutrition</jtitle><addtitle>J Nutr</addtitle><date>1990-09-01</date><risdate>1990</risdate><volume>120</volume><issue>9</issue><spage>1075</spage><epage>1079</epage><pages>1075-1079</pages><issn>0022-3166</issn><eissn>1541-6100</eissn><coden>JONUAI</coden><abstract>The effect of manganese deficiency on insulin binding, glucose transport and metabolism in isolated adipose cells from Sprague-Dawley rats was investigated. Offspring from Mn-sufficient female rats fed 45 µg Mn/g diet (control) and from Mn-deficient (Mn-) female rats fed 1 µg Mn/g diet were used in these studies. Both basal and insulin-stimulated 3-O-methylglucose transport in isolated adipose cells was significantly lower in Mn- rats, averaging 40% and 50% of control values, respectively. Kinetic analysis of glucose transport demonstrated a lower maximal transport velocity (Vmax) for glucose in adipose cells from Mn- rats compared to controls. No differences in the Km for glucose uptake were observed between the two groups. Insulin-stimulated glucose oxidation to CO2 and conversion to triglycerides was lower in isolated adipose cells from Mn- rats compared to controls. Mn- animals had fewer insulin receptors per cell compared to controls, although no differences in insulin receptor affinity were observed between the two groups. These data suggest that Mn deficiency affects glucose transport and metabolism in the adipose cell. The apparent defect lies distal to the insulin receptor and probably reflects a decreased number of glucose transporters in adipose tissue of Mn- rats.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>2204694</pmid><doi>10.1093/jn/120.9.1075</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | adipocytes Adipose Tissue - cytology Adipose Tissue - metabolism Animals Biological and medical sciences Biological Transport, Active Carbohydrates carence minerale cells Cells, Cultured cellule celulas deficiencia de minerales diet dieta Female Fundamental and applied biological sciences. Psychology glucosa glucose Glucose - metabolism glucose transport Insulin - metabolism insulin receptor Kinetics manganese Manganese - deficiency manganeso metabolism metabolisme metabolismo Metabolisms and neurohumoral controls mineral deficiencies nutrient transport Pregnancy rat rata Rats Rats, Inbred Strains regime alimentaire transport des substances nutritives transporte de nutrientes Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Effect of Manganese Deficiency on Insulin Binding, Glucose Transport and Metabolism in Rat Adipocytes |
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