Application of free-flow electrophoresis for isolation and purification of proteins and peptides

Free‐flow electrophoresis (FFE) has been applied to the separation and purification of a variety of proteins and polypeptides: bee venom, tumor necrosis factor, interleukin‐1β, interferon‐α and superoxide dismutase. FFE at constant pH and conductivity of the carrying buffer is shown to be efficient...

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Veröffentlicht in:	Electrophoresis 1998-06, Vol.19 (7), p.1127-1134
Hauptverfasser:	Loseva, Olga I., Gavryushkin, Alexander V., Osipov, Vladimir V., Vanyakin, Evgeny N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apamin - isolation & purification Bees - chemistry Cattle Electrophoresis - instrumentation Electrophoresis - methods Free-flow electrophoresis Hyaluronoglucosaminidase - isolation & purification Interferon-gamma - isolation & purification Interleukin-1 - isolation & purification Melitten - isolation & purification Peptides - isolation & purification Phospholipases A - isolation & purification Preparative electrophoresis Protein purification Proteins - isolation & purification Superoxide Dismutase - isolation & purification Tumor Necrosis Factor-alpha - isolation & purification
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