Characterization, cloning and host-protective activity of a 30-kilodalton glycoprotein secreted by the parasitic stages of Trichostrongylus colubriformis
The helminth Trichostrongylus colubriformis is a parasitic nematode infecting the small intestine of sheep. We report the isolation and characterization of a 30-kDa glycoprotein capable of partially protecting guinea-pigs against the parasite. This glycoprotein is secreted by the L4 and adult parasi...
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| Veröffentlicht in: | Molecular and biochemical parasitology 1990, Vol.41 (2), p.167-176 |
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| container_title | Molecular and biochemical parasitology |
| container_volume | 41 |
| creator | Savin, Keith W. Dopheide, Theo A.A. Frenkel, Maurice J. Wagland, Barry M. Grant, Warwick N. Ward, Colin W. |
| description | The helminth
Trichostrongylus colubriformis is a parasitic nematode infecting the small intestine of sheep. We report the isolation and characterization of a 30-kDa glycoprotein capable of partially protecting guinea-pigs against the parasite. This glycoprotein is secreted by the L4 and adult parasitic stages of the worm. The sequence of three separate cDNA clones predicts the polypeptide to be about 15 kDa, with four
N-linked carbohydrate chains and an internal disulphide bond. The clones also indicate the existence of sequence variability in this antigen. Limited sequence homology to a porcine intestinal peptide suggests an influence on host gut physiology. |
| doi_str_mv | 10.1016/0166-6851(90)90179-P |
| format | Article |
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Trichostrongylus colubriformis is a parasitic nematode infecting the small intestine of sheep. We report the isolation and characterization of a 30-kDa glycoprotein capable of partially protecting guinea-pigs against the parasite. This glycoprotein is secreted by the L4 and adult parasitic stages of the worm. The sequence of three separate cDNA clones predicts the polypeptide to be about 15 kDa, with four
N-linked carbohydrate chains and an internal disulphide bond. The clones also indicate the existence of sequence variability in this antigen. Limited sequence homology to a porcine intestinal peptide suggests an influence on host gut physiology.</description><identifier>ISSN: 0166-6851</identifier><identifier>EISSN: 1872-9428</identifier><identifier>DOI: 10.1016/0166-6851(90)90179-P</identifier><identifier>PMID: 2204828</identifier><identifier>CODEN: MBIPDP</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Animals ; Antigens, Helminth - genetics ; Antigens, Helminth - immunology ; Antigens, Helminth - isolation & purification ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; clones ; Cloning, Molecular ; Disulfides ; DNA ; DNA - genetics ; Escherichia coli - genetics ; Expression ; Fundamental and applied biological sciences. Psychology ; Gene ; Genes. Genome ; Glycoprotein ; glycoproteins ; Glycoproteins - genetics ; Glycoproteins - immunology ; Glycoproteins - isolation & purification ; Guinea Pigs ; Helminth Proteins - genetics ; Helminth Proteins - immunology ; Helminth Proteins - isolation & purification ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Nematode ; nucleotide sequences ; RNA, Messenger - biosynthesis ; Secretion ; sheep ; Trichostrongyloidiasis - immunology ; Trichostrongylosis - immunology ; Trichostrongylus ; Trichostrongylus - genetics ; Trichostrongylus - immunology ; Trichostrongylus - metabolism ; Trichostrongylus colubriformis ; Vaccines - immunology</subject><ispartof>Molecular and biochemical parasitology, 1990, Vol.41 (2), p.167-176</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-34e5956036c7452b76554265f967ca8ea9f498e9c35b675448eac1343305c1243</citedby><cites>FETCH-LOGICAL-c442t-34e5956036c7452b76554265f967ca8ea9f498e9c35b675448eac1343305c1243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0166-6851(90)90179-P$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19521670$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2204828$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Savin, Keith W.</creatorcontrib><creatorcontrib>Dopheide, Theo A.A.</creatorcontrib><creatorcontrib>Frenkel, Maurice J.</creatorcontrib><creatorcontrib>Wagland, Barry M.</creatorcontrib><creatorcontrib>Grant, Warwick N.</creatorcontrib><creatorcontrib>Ward, Colin W.</creatorcontrib><title>Characterization, cloning and host-protective activity of a 30-kilodalton glycoprotein secreted by the parasitic stages of Trichostrongylus colubriformis</title><title>Molecular and biochemical parasitology</title><addtitle>Mol Biochem Parasitol</addtitle><description>The helminth
Trichostrongylus colubriformis is a parasitic nematode infecting the small intestine of sheep. We report the isolation and characterization of a 30-kDa glycoprotein capable of partially protecting guinea-pigs against the parasite. This glycoprotein is secreted by the L4 and adult parasitic stages of the worm. The sequence of three separate cDNA clones predicts the polypeptide to be about 15 kDa, with four
N-linked carbohydrate chains and an internal disulphide bond. The clones also indicate the existence of sequence variability in this antigen. Limited sequence homology to a porcine intestinal peptide suggests an influence on host gut physiology.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Animals</subject><subject>Antigens, Helminth - genetics</subject><subject>Antigens, Helminth - immunology</subject><subject>Antigens, Helminth - isolation & purification</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>clones</subject><subject>Cloning, Molecular</subject><subject>Disulfides</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>Escherichia coli - genetics</subject><subject>Expression</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene</subject><subject>Genes. Genome</subject><subject>Glycoprotein</subject><subject>glycoproteins</subject><subject>Glycoproteins - genetics</subject><subject>Glycoproteins - immunology</subject><subject>Glycoproteins - isolation & purification</subject><subject>Guinea Pigs</subject><subject>Helminth Proteins - genetics</subject><subject>Helminth Proteins - immunology</subject><subject>Helminth Proteins - isolation & purification</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nematode</subject><subject>nucleotide sequences</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Secretion</subject><subject>sheep</subject><subject>Trichostrongyloidiasis - immunology</subject><subject>Trichostrongylosis - immunology</subject><subject>Trichostrongylus</subject><subject>Trichostrongylus - genetics</subject><subject>Trichostrongylus - immunology</subject><subject>Trichostrongylus - metabolism</subject><subject>Trichostrongylus colubriformis</subject><subject>Vaccines - immunology</subject><issn>0166-6851</issn><issn>1872-9428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd2KFDEQhRtR1tnVN1DMjaJga_7TuRFk8A8WXHD3OqTT1TPRns5skl5o32Tf1vTOsN7pRShIfXVOUaeqnhH8jmAi35cna9kI8lrjNxoTpeuLB9WKNIrWmtPmYbW6Rx5Xpyn9xBgLJeVJdUIp5g1tVtXtemujdRmi_22zD-Nb5IYw-nGD7NihbUi53seQwWV_A8guxecZhR5ZxHD9yw-hs0MOI9oMswt3rB9RAhchQ4faGeUtoH1xST57h1K2G0iLwGX0bjGIYdzMw5SQC8PURt-HuPPpSfWot0OCp8d6Vl19_nS5_lqff__ybf3xvHac01wzDkILiZl0igvaKikEp1L0WipnG7C657oB7ZhopRKcly9HGGcMC0coZ2fVq4NuWf16gpRNMXcwDHaEMCWjtNakIfK_IBEaayZVAfkBdDGkFKE3--h3Ns6GYLNEZ5ZczJKL0djcRWcuytjzo_7U7qC7HzpmVfovj32bnB36aEfn019tLSiRChfuxYHrbTB2Ewtz9YNiwjBVWBWtQnw4EFDueuMhmuQ8jA46H0vQpgv-36v-AfpbwPA</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Savin, Keith W.</creator><creator>Dopheide, Theo A.A.</creator><creator>Frenkel, Maurice J.</creator><creator>Wagland, Barry M.</creator><creator>Grant, Warwick N.</creator><creator>Ward, Colin W.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>Characterization, cloning and host-protective activity of a 30-kilodalton glycoprotein secreted by the parasitic stages of Trichostrongylus colubriformis</title><author>Savin, Keith W. ; Dopheide, Theo A.A. ; Frenkel, Maurice J. ; Wagland, Barry M. ; Grant, Warwick N. ; Ward, Colin W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-34e5956036c7452b76554265f967ca8ea9f498e9c35b675448eac1343305c1243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Animals</topic><topic>Antigens, Helminth - genetics</topic><topic>Antigens, Helminth - immunology</topic><topic>Antigens, Helminth - isolation & purification</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>clones</topic><topic>Cloning, Molecular</topic><topic>Disulfides</topic><topic>DNA</topic><topic>DNA - genetics</topic><topic>Escherichia coli - genetics</topic><topic>Expression</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene</topic><topic>Genes. Genome</topic><topic>Glycoprotein</topic><topic>glycoproteins</topic><topic>Glycoproteins - genetics</topic><topic>Glycoproteins - immunology</topic><topic>Glycoproteins - isolation & purification</topic><topic>Guinea Pigs</topic><topic>Helminth Proteins - genetics</topic><topic>Helminth Proteins - immunology</topic><topic>Helminth Proteins - isolation & purification</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nematode</topic><topic>nucleotide sequences</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Secretion</topic><topic>sheep</topic><topic>Trichostrongyloidiasis - immunology</topic><topic>Trichostrongylosis - immunology</topic><topic>Trichostrongylus</topic><topic>Trichostrongylus - genetics</topic><topic>Trichostrongylus - immunology</topic><topic>Trichostrongylus - metabolism</topic><topic>Trichostrongylus colubriformis</topic><topic>Vaccines - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Savin, Keith W.</creatorcontrib><creatorcontrib>Dopheide, Theo A.A.</creatorcontrib><creatorcontrib>Frenkel, Maurice J.</creatorcontrib><creatorcontrib>Wagland, Barry M.</creatorcontrib><creatorcontrib>Grant, Warwick N.</creatorcontrib><creatorcontrib>Ward, Colin W.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and biochemical parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Savin, Keith W.</au><au>Dopheide, Theo A.A.</au><au>Frenkel, Maurice J.</au><au>Wagland, Barry M.</au><au>Grant, Warwick N.</au><au>Ward, Colin W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization, cloning and host-protective activity of a 30-kilodalton glycoprotein secreted by the parasitic stages of Trichostrongylus colubriformis</atitle><jtitle>Molecular and biochemical parasitology</jtitle><addtitle>Mol Biochem Parasitol</addtitle><date>1990</date><risdate>1990</risdate><volume>41</volume><issue>2</issue><spage>167</spage><epage>176</epage><pages>167-176</pages><issn>0166-6851</issn><eissn>1872-9428</eissn><coden>MBIPDP</coden><abstract>The helminth
Trichostrongylus colubriformis is a parasitic nematode infecting the small intestine of sheep. We report the isolation and characterization of a 30-kDa glycoprotein capable of partially protecting guinea-pigs against the parasite. This glycoprotein is secreted by the L4 and adult parasitic stages of the worm. The sequence of three separate cDNA clones predicts the polypeptide to be about 15 kDa, with four
N-linked carbohydrate chains and an internal disulphide bond. The clones also indicate the existence of sequence variability in this antigen. Limited sequence homology to a porcine intestinal peptide suggests an influence on host gut physiology.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>2204828</pmid><doi>10.1016/0166-6851(90)90179-P</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-6851 |
| ispartof | Molecular and biochemical parasitology, 1990, Vol.41 (2), p.167-176 |
| issn | 0166-6851 1872-9428 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Amino Acid Sequence amino acid sequences Animals Antigens, Helminth - genetics Antigens, Helminth - immunology Antigens, Helminth - isolation & purification Base Sequence Biological and medical sciences Blotting, Northern clones Cloning, Molecular Disulfides DNA DNA - genetics Escherichia coli - genetics Expression Fundamental and applied biological sciences. Psychology Gene Genes. Genome Glycoprotein glycoproteins Glycoproteins - genetics Glycoproteins - immunology Glycoproteins - isolation & purification Guinea Pigs Helminth Proteins - genetics Helminth Proteins - immunology Helminth Proteins - isolation & purification Molecular and cellular biology Molecular genetics Molecular Sequence Data Nematode nucleotide sequences RNA, Messenger - biosynthesis Secretion sheep Trichostrongyloidiasis - immunology Trichostrongylosis - immunology Trichostrongylus Trichostrongylus - genetics Trichostrongylus - immunology Trichostrongylus - metabolism Trichostrongylus colubriformis Vaccines - immunology |
| title | Characterization, cloning and host-protective activity of a 30-kilodalton glycoprotein secreted by the parasitic stages of Trichostrongylus colubriformis |
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