Human occult loiasis: field evaluation of a nested polymerase chain reaction assay for the detection of occult infection

A nested polymerase chain reaction (nested PCR) assay, targeted on the repeat 3 region (15r3) of the gene coding for a Loaloa 15 kD polyprotein, was developed to detect L. loa infection. The assay has a sensitivity of 95% and is 100% specific with regard to sympatric filarial parasites: Mansonella p...

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Veröffentlicht in:	Tropical medicine & international health 1998-06, Vol.3 (6), p.505-511
Hauptverfasser:	Touré, Fousseyni S., Mavoungou, Elie, Kassambara, Lalla, Williams, Tim, Wahl, Goetz, Egwang, Thomas G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Aged Aged, 80 and over Biological and medical sciences Child diagnosis Diagnosis, Differential Diseases caused by nematodes DNA Primers Female Filariases Gabon - epidemiology Helminthic diseases Humans Infectious diseases Loaiasis Loiasis - diagnosis Loiasis - epidemiology Male Medical sciences Middle Aged nested polymerase chain reaction & Field evaluation Occult loiasis Parasitic diseases Polymerase Chain Reaction - methods Prevalence Sensitivity and Specificity Tropical medicine
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