An initiation zone of chromosomal DNA replication at the chicken lysozyme gene locus
The chicken lysozyme gene domain is distinguished by a broad knowledge of how its expression is regulated. Here, we examined the in vivo replication of the lysozyme gene locus using polymerase chain reaction amplification and competitive polymerase chain reaction of size-fractionated, nascent DNA st...
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| Veröffentlicht in: | The Journal of biological chemistry 1998-07, Vol.273 (29), p.18300-18307 |
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| container_issue | 29 |
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| container_title | The Journal of biological chemistry |
| container_volume | 273 |
| creator | Phi-van, L. (Institut fur Tierzucht und Tieverhalten, Celle, Germany) Selke, C Bodenhausen, A. von Stratling, W.H |
| description | The chicken lysozyme gene domain is distinguished by a broad knowledge of how its expression is regulated. Here, we examined
the in vivo replication of the lysozyme gene locus using polymerase chain reaction amplification and competitive polymerase chain reaction
of size-fractionated, nascent DNA strands. We found that DNA replication initiates at multiple sites within a broad initiation
zone spanning at least 20 kilobases, which includes most of the lysozyme gene domain. The 5â² border of this zone is probably
located downstream of the lysozyme 5â² nuclear matrix attachment region. Preferred initiation occurs in a 3â²-located subzone.
The initiation zone at the lysozyme gene locus is also active in nonexpressing liver DU249 cells. Furthermore, examining the
timing of DNA replication at the lysozyme gene locus revealed that the gene locus replicates early during S phase in both
HD11 and DU249 cells, irrespective of its transcriptional activity. |
| doi_str_mv | 10.1074/jbc.273.29.18300 |
| format | Article |
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the in vivo replication of the lysozyme gene locus using polymerase chain reaction amplification and competitive polymerase chain reaction
of size-fractionated, nascent DNA strands. We found that DNA replication initiates at multiple sites within a broad initiation
zone spanning at least 20 kilobases, which includes most of the lysozyme gene domain. The 5â² border of this zone is probably
located downstream of the lysozyme 5â² nuclear matrix attachment region. Preferred initiation occurs in a 3â²-located subzone.
The initiation zone at the lysozyme gene locus is also active in nonexpressing liver DU249 cells. Furthermore, examining the
timing of DNA replication at the lysozyme gene locus revealed that the gene locus replicates early during S phase in both
HD11 and DU249 cells, irrespective of its transcriptional activity.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.273.29.18300</identifier><identifier>PMID: 9660795</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Animals ; ARN MENSAJERO ; ARN MESSAGER ; CELL CULTURE ; CELL DIVISION ; CELL LINES ; Cells, Cultured ; CHICKENS ; Chromosomes - metabolism ; Codon, Initiator ; CULTIVO DE CELULAS ; CULTURE DE CELLULE ; DIVISION CELLULAIRE ; DIVISION CELULAR ; DNA Replication ; EXPRESION GENICA ; EXPRESSION DES GENES ; FOIE ; GENE ; GENE EXPRESSION ; GENES ; Genes, myc ; HIGADO ; INTERPHASE ; LISOZIMA ; LIVER ; Liver - enzymology ; LYSOZYME ; MESSENGER RNA ; Muramidase - genetics ; POLLO ; Polymerase Chain Reaction ; POULET ; REPLICACION ; REPLICATION ; S Phase ; Transcription, Genetic</subject><ispartof>The Journal of biological chemistry, 1998-07, Vol.273 (29), p.18300-18307</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-fa56daa863d063293f8aa00934838da5f1bf00e9aa39fb7e91d3da98202eb793</citedby><cites>FETCH-LOGICAL-c418t-fa56daa863d063293f8aa00934838da5f1bf00e9aa39fb7e91d3da98202eb793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9660795$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Phi-van, L. (Institut fur Tierzucht und Tieverhalten, Celle, Germany)</creatorcontrib><creatorcontrib>Selke, C</creatorcontrib><creatorcontrib>Bodenhausen, A. von</creatorcontrib><creatorcontrib>Stratling, W.H</creatorcontrib><title>An initiation zone of chromosomal DNA replication at the chicken lysozyme gene locus</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The chicken lysozyme gene domain is distinguished by a broad knowledge of how its expression is regulated. Here, we examined
the in vivo replication of the lysozyme gene locus using polymerase chain reaction amplification and competitive polymerase chain reaction
of size-fractionated, nascent DNA strands. We found that DNA replication initiates at multiple sites within a broad initiation
zone spanning at least 20 kilobases, which includes most of the lysozyme gene domain. The 5â² border of this zone is probably
located downstream of the lysozyme 5â² nuclear matrix attachment region. Preferred initiation occurs in a 3â²-located subzone.
The initiation zone at the lysozyme gene locus is also active in nonexpressing liver DU249 cells. Furthermore, examining the
timing of DNA replication at the lysozyme gene locus revealed that the gene locus replicates early during S phase in both
HD11 and DU249 cells, irrespective of its transcriptional activity.</description><subject>Animals</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>CELL CULTURE</subject><subject>CELL DIVISION</subject><subject>CELL LINES</subject><subject>Cells, Cultured</subject><subject>CHICKENS</subject><subject>Chromosomes - metabolism</subject><subject>Codon, Initiator</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>DIVISION CELLULAIRE</subject><subject>DIVISION CELULAR</subject><subject>DNA Replication</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>FOIE</subject><subject>GENE</subject><subject>GENE EXPRESSION</subject><subject>GENES</subject><subject>Genes, myc</subject><subject>HIGADO</subject><subject>INTERPHASE</subject><subject>LISOZIMA</subject><subject>LIVER</subject><subject>Liver - enzymology</subject><subject>LYSOZYME</subject><subject>MESSENGER RNA</subject><subject>Muramidase - genetics</subject><subject>POLLO</subject><subject>Polymerase Chain Reaction</subject><subject>POULET</subject><subject>REPLICACION</subject><subject>REPLICATION</subject><subject>S Phase</subject><subject>Transcription, Genetic</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtvFDEUhS0ECkugp0Fygehmc23Pw7dchacUhSIbic7yeK53HGbGiz0rtPn1DOyKDnGbW5zvnOJj7LWAtYCmvHpo3Vo2ai1xLbQCeMJWArQqVCW-PWUrACkKlJV-zl7k_ADLlSgu2AXWNTRYrdh2M_EwhTnYOcSJP8aJePTc9SmOMcfRDvz97YYn2g_BnRg787mnBQnuO018OOb4eByJ72jpDtEd8kv2zNsh06vzv2Tbjx-215-Lm6-fvlxvbgpXCj0X3lZ1Z62uVQe1kqi8thYAVamV7mzlResBCK1V6NuGUHSqs6glSGobVJfs3Wl2n-KPA-XZjCE7GgY7UTxk0yBqrCX8FxR1VSLUzQLCCXQp5pzIm30Ko01HI8D8Fm4W4WYRbiSaP8KXypvz9qEdqftbOBte8renvA-7_mdIZNoQXU_jP2a8jcbuUsjm_k4gNoBQgVK_APc7kOA</recordid><startdate>19980717</startdate><enddate>19980717</enddate><creator>Phi-van, L. (Institut fur Tierzucht und Tieverhalten, Celle, Germany)</creator><creator>Selke, C</creator><creator>Bodenhausen, A. von</creator><creator>Stratling, W.H</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19980717</creationdate><title>An initiation zone of chromosomal DNA replication at the chicken lysozyme gene locus</title><author>Phi-van, L. (Institut fur Tierzucht und Tieverhalten, Celle, Germany) ; Selke, C ; Bodenhausen, A. von ; Stratling, W.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-fa56daa863d063293f8aa00934838da5f1bf00e9aa39fb7e91d3da98202eb793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>CELL CULTURE</topic><topic>CELL DIVISION</topic><topic>CELL LINES</topic><topic>Cells, Cultured</topic><topic>CHICKENS</topic><topic>Chromosomes - metabolism</topic><topic>Codon, Initiator</topic><topic>CULTIVO DE CELULAS</topic><topic>CULTURE DE CELLULE</topic><topic>DIVISION CELLULAIRE</topic><topic>DIVISION CELULAR</topic><topic>DNA Replication</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>FOIE</topic><topic>GENE</topic><topic>GENE EXPRESSION</topic><topic>GENES</topic><topic>Genes, myc</topic><topic>HIGADO</topic><topic>INTERPHASE</topic><topic>LISOZIMA</topic><topic>LIVER</topic><topic>Liver - enzymology</topic><topic>LYSOZYME</topic><topic>MESSENGER RNA</topic><topic>Muramidase - genetics</topic><topic>POLLO</topic><topic>Polymerase Chain Reaction</topic><topic>POULET</topic><topic>REPLICACION</topic><topic>REPLICATION</topic><topic>S Phase</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Phi-van, L. (Institut fur Tierzucht und Tieverhalten, Celle, Germany)</creatorcontrib><creatorcontrib>Selke, C</creatorcontrib><creatorcontrib>Bodenhausen, A. von</creatorcontrib><creatorcontrib>Stratling, W.H</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Phi-van, L. (Institut fur Tierzucht und Tieverhalten, Celle, Germany)</au><au>Selke, C</au><au>Bodenhausen, A. von</au><au>Stratling, W.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An initiation zone of chromosomal DNA replication at the chicken lysozyme gene locus</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1998-07-17</date><risdate>1998</risdate><volume>273</volume><issue>29</issue><spage>18300</spage><epage>18307</epage><pages>18300-18307</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>The chicken lysozyme gene domain is distinguished by a broad knowledge of how its expression is regulated. Here, we examined
the in vivo replication of the lysozyme gene locus using polymerase chain reaction amplification and competitive polymerase chain reaction
of size-fractionated, nascent DNA strands. We found that DNA replication initiates at multiple sites within a broad initiation
zone spanning at least 20 kilobases, which includes most of the lysozyme gene domain. The 5â² border of this zone is probably
located downstream of the lysozyme 5â² nuclear matrix attachment region. Preferred initiation occurs in a 3â²-located subzone.
The initiation zone at the lysozyme gene locus is also active in nonexpressing liver DU249 cells. Furthermore, examining the
timing of DNA replication at the lysozyme gene locus revealed that the gene locus replicates early during S phase in both
HD11 and DU249 cells, irrespective of its transcriptional activity.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>9660795</pmid><doi>10.1074/jbc.273.29.18300</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1998-07, Vol.273 (29), p.18300-18307 |
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| language | eng |
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| source | Electronic Journals Library; MEDLINE; Alma/SFX Local Collection |
| subjects | Animals ARN MENSAJERO ARN MESSAGER CELL CULTURE CELL DIVISION CELL LINES Cells, Cultured CHICKENS Chromosomes - metabolism Codon, Initiator CULTIVO DE CELULAS CULTURE DE CELLULE DIVISION CELLULAIRE DIVISION CELULAR DNA Replication EXPRESION GENICA EXPRESSION DES GENES FOIE GENE GENE EXPRESSION GENES Genes, myc HIGADO INTERPHASE LISOZIMA LIVER Liver - enzymology LYSOZYME MESSENGER RNA Muramidase - genetics POLLO Polymerase Chain Reaction POULET REPLICACION REPLICATION S Phase Transcription, Genetic |
| title | An initiation zone of chromosomal DNA replication at the chicken lysozyme gene locus |
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