Cloning, purification and characterisation of the lipase from Staphylococcus epidermidis--comparison of the substrate selectivity with those of other microbial lipases

On the chromosome of Staphylococcus epidermidis RP62A the lipase gene (gehSE1) is immediately flanked by the icaAA'BC operon, which is involved in biofilm formation. Since lipase production might play a role in staphylococcal skin colonisation as well, we studied the biochemical properties of t...

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Veröffentlicht in:	European journal of biochemistry 1998-05, Vol.253 (3), p.675-683
Hauptverfasser:	Simons, J W, van Kampen, M D, Riel, S, Götz, F, Egmond, M R, Verheij, H M
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Cloning, Molecular Conserved Sequence DNA Primers Fungi - enzymology gehSE1 gene Genomic Library Gram-Negative Bacteria - enzymology Gram-Positive Bacteria - enzymology icaAA'BC operon Kinetics Lipase - chemistry Lipase - isolation & purification Lipase - metabolism Molecular Sequence Data Plasmids Polymerase Chain Reaction Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Sequence Alignment Sequence Homology, Amino Acid Species Specificity Staphylococcus aureus Staphylococcus epidermidis - enzymology Staphylococcus epidermidis - genetics Substrate Specificity
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