Measurement of Abundance Ratio of 15N in Amino Acids by Capillary Gas Chromatography-Mass Spectrometry

Measurement of Abundance Ratio of 15N in Amino Acids by Capillary Gas Chromatography-Mass Spectrometry

A capillary gas chromatography-mass spectrometry for analysis of 15N-labeled amino acids and amides is described. The method is based on direct silylation of amino acidsand amides with MTBSTFA and the formation of the TBDMS derivatives. The method was possible simultaneously to measure the 15N abund...

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Veröffentlicht in:RADIOISOTOPES 1990/05/15, Vol.39(5), pp.204-211
Hauptverfasser: SAMUKAWA, Kisaburo, ITANO, Morihide, TAKINO, Masahiko, KOMAI, Yutaka
Format: Artikel
Sprache:jpn
Schlagworte:
amide
amino acids
Amino Acids - analysis
capillary column gas chromatography-mass spectrometry
Gas Chromatography-Mass Spectrometry
Glutamine - analysis
Nitrogen Isotopes
nitrogen-15 abundance ratio
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Zusammenfassung:A capillary gas chromatography-mass spectrometry for analysis of 15N-labeled amino acids and amides is described. The method is based on direct silylation of amino acidsand amides with MTBSTFA and the formation of the TBDMS derivatives. The method was possible simultaneously to measure the 15N abundance ratio of amino-N and amide-N of amides, as to analysis of amino acids.
ISSN:0033-8303
1884-4111
DOI:10.3769/radioisotopes.39.5_204