Primary Culture of Normal Rat Mammary Epithelial Cells within a Basement Membrane Matrix. I. Regulation of Proliferation by Hormones and Growth Factors

A serum-free primary culture system has been developed which allows for three-dimensional growth and differentiation of normal rat mammary epithelial cells (RMECs) within an extracellular matrix preparation. RMECs were isolated from mammary glands of immature 50- to 60-d-old rats and the organoids e...

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Veröffentlicht in:	In Vitro Cellular &Developmental Biology 1990-08, Vol.26 (8), p.791-802
Hauptverfasser:	Hahm, H A, Ip, M M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Ascorbic Acid - pharmacology Basement membrane Basement Membrane - physiology Cell Differentiation Cell Division - drug effects Cell growth Cells, Cultured Collagen Collagens Cultured cells Epithelial Cells epithelium Extracellular Matrix - physiology Gels growth factors Growth Substances - pharmacology Hormones Hormones - pharmacology In Vitro Techniques Insulin Lactation Mammary glands Mammary Glands, Animal - cytology Rats Transferrin - pharmacology Transferrins
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