Interaction of mitochondrial RNA editing and nucleolytic processing in the restoration of male fertility in sorghum
Nucleolytic processing of transcripts within mitochondrial orf107, associated with male sterility in sorghum, is regulated by the fertility restoration gene Rf3, conferring 75% cleavage of whole-length transcripts. Two transcript editing sites are 81% and 61% edited in rf3rf3 lines, while these site...
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| Veröffentlicht in: | Current genetics 1998-06, Vol.33 (6), p.429-436 |
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| creator | Pring, D.R Chen, W Tang, H.V Howad, W Kempken, F |
| description | Nucleolytic processing of transcripts within mitochondrial orf107, associated with male sterility in sorghum, is regulated by the fertility restoration gene Rf3, conferring 75% cleavage of whole-length transcripts. Two transcript editing sites are 81% and 61% edited in rf3rf3 lines, while these sites are 41% and 10% edited in the remaining whole-length transcripts in an Rf3Rf3 line. RNA editing and processing efficiency in F1 progeny were similar to the Rf3Rf3 parent, and analyses of backcross progeny indicated that all rf3rf3 lines were characterized by high editing efficiency. We postulate that highly edited transcripts within the population are quickly processed in lines carrying Rf3, generating a residual population of poorly edited transcripts. Thus, action of Rf3 may have no direct affect on RNA editing, and may be dependent on a substrate of highly edited transcripts. These data indicate a potentially novel role of RNA editing in gene expression through an influence on the efficiency of transcript processing. |
| doi_str_mv | 10.1007/s002940050356 |
| format | Article |
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Two transcript editing sites are 81% and 61% edited in rf3rf3 lines, while these sites are 41% and 10% edited in the remaining whole-length transcripts in an Rf3Rf3 line. RNA editing and processing efficiency in F1 progeny were similar to the Rf3Rf3 parent, and analyses of backcross progeny indicated that all rf3rf3 lines were characterized by high editing efficiency. We postulate that highly edited transcripts within the population are quickly processed in lines carrying Rf3, generating a residual population of poorly edited transcripts. Thus, action of Rf3 may have no direct affect on RNA editing, and may be dependent on a substrate of highly edited transcripts. 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Two transcript editing sites are 81% and 61% edited in rf3rf3 lines, while these sites are 41% and 10% edited in the remaining whole-length transcripts in an Rf3Rf3 line. RNA editing and processing efficiency in F1 progeny were similar to the Rf3Rf3 parent, and analyses of backcross progeny indicated that all rf3rf3 lines were characterized by high editing efficiency. We postulate that highly edited transcripts within the population are quickly processed in lines carrying Rf3, generating a residual population of poorly edited transcripts. Thus, action of Rf3 may have no direct affect on RNA editing, and may be dependent on a substrate of highly edited transcripts. These data indicate a potentially novel role of RNA editing in gene expression through an influence on the efficiency of transcript processing.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Binding Sites - genetics</subject><subject>c-to-u rna editing</subject><subject>Cell Nucleus - chemistry</subject><subject>Cell Nucleus - genetics</subject><subject>complementary DNA</subject><subject>cytoplasmic male sterility</subject><subject>DNA, Mitochondrial - genetics</subject><subject>Edible Grain - chemistry</subject><subject>Edible Grain - genetics</subject><subject>Edible Grain - physiology</subject><subject>Fertility - genetics</subject><subject>Fertility - physiology</subject><subject>Genes, Plant - genetics</subject><subject>genomics</subject><subject>Heterozygote</subject><subject>line differences</subject><subject>male fertility</subject><subject>messenger RNA</subject><subject>Mitochondria - chemistry</subject><subject>Mitochondria - genetics</subject><subject>mitochondrial DNA</subject><subject>molecular conformation</subject><subject>Molecular Sequence Data</subject><subject>Molecular Structure</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - physiology</subject><subject>nucleases</subject><subject>Nucleic Acid Conformation</subject><subject>Open Reading Frames - genetics</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - physiology</subject><subject>protein secondary structure</subject><subject>restorer genes</subject><subject>rf3 gene</subject><subject>RNA - genetics</subject><subject>RNA - metabolism</subject><subject>RNA editing</subject><subject>RNA Editing - genetics</subject><subject>RNA Editing - physiology</subject><subject>RNA, Mitochondrial</subject><subject>Sorghum bicolor</subject><subject>Transcription, Genetic - genetics</subject><issn>0172-8083</issn><issn>1432-0983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EKtvCkSPCJ26BGdtx7GNVFahUgQT0HHmdya5REhfbOey_x6tdVeLU0UhzeB-988XYO4RPCNB9zgDCKoAWZKtfsA0qKRqwRr5kG8BONAaMfM0uc_4DgMLY7oJdWK2UAL1h-W4plJwvIS48jnwOJfp9XIYU3MR_fr_mNIQSlh13y8CX1U8Up0MJnj-m6CnnoxQWXvbEE-USk3uychPxkVIJUyiHI5Rj2u3X-Q17Nbop09tzvWIPX25_33xr7n98vbu5vm-8VLI06IxWWg_eovHCbbUwLWpSwtetCGr4rTLYIVLXum6rrZej8-hIIZkR5BX7ePKto_5d63D9HLKnaXILxTX3nbU1lX0WrD2UVEZWsDmBPsWcE439YwqzS4ceoT9-o__vG5V_fzZetzMNT_T5_FX_cNJHF3u3SyH3D78EoKwmiLJV8h-YX47l</recordid><startdate>19980601</startdate><enddate>19980601</enddate><creator>Pring, D.R</creator><creator>Chen, W</creator><creator>Tang, H.V</creator><creator>Howad, W</creator><creator>Kempken, F</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19980601</creationdate><title>Interaction of mitochondrial RNA editing and nucleolytic processing in the restoration of male fertility in sorghum</title><author>Pring, D.R ; Chen, W ; Tang, H.V ; Howad, W ; Kempken, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-1a86466dc918c2ab628516e42c172e0000cb481711e75a7b69c3fac1ae41e8f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Binding Sites - genetics</topic><topic>c-to-u rna editing</topic><topic>Cell Nucleus - chemistry</topic><topic>Cell Nucleus - genetics</topic><topic>complementary DNA</topic><topic>cytoplasmic male sterility</topic><topic>DNA, Mitochondrial - genetics</topic><topic>Edible Grain - chemistry</topic><topic>Edible Grain - genetics</topic><topic>Edible Grain - physiology</topic><topic>Fertility - genetics</topic><topic>Fertility - physiology</topic><topic>Genes, Plant - genetics</topic><topic>genomics</topic><topic>Heterozygote</topic><topic>line differences</topic><topic>male fertility</topic><topic>messenger RNA</topic><topic>Mitochondria - chemistry</topic><topic>Mitochondria - genetics</topic><topic>mitochondrial DNA</topic><topic>molecular conformation</topic><topic>Molecular Sequence Data</topic><topic>Molecular Structure</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - physiology</topic><topic>nucleases</topic><topic>Nucleic Acid Conformation</topic><topic>Open Reading Frames - genetics</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - physiology</topic><topic>protein secondary structure</topic><topic>restorer genes</topic><topic>rf3 gene</topic><topic>RNA - genetics</topic><topic>RNA - metabolism</topic><topic>RNA editing</topic><topic>RNA Editing - genetics</topic><topic>RNA Editing - physiology</topic><topic>RNA, Mitochondrial</topic><topic>Sorghum bicolor</topic><topic>Transcription, Genetic - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pring, D.R</creatorcontrib><creatorcontrib>Chen, W</creatorcontrib><creatorcontrib>Tang, H.V</creatorcontrib><creatorcontrib>Howad, W</creatorcontrib><creatorcontrib>Kempken, F</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pring, D.R</au><au>Chen, W</au><au>Tang, H.V</au><au>Howad, W</au><au>Kempken, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interaction of mitochondrial RNA editing and nucleolytic processing in the restoration of male fertility in sorghum</atitle><jtitle>Current genetics</jtitle><addtitle>Curr Genet</addtitle><date>1998-06-01</date><risdate>1998</risdate><volume>33</volume><issue>6</issue><spage>429</spage><epage>436</epage><pages>429-436</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><abstract>Nucleolytic processing of transcripts within mitochondrial orf107, associated with male sterility in sorghum, is regulated by the fertility restoration gene Rf3, conferring 75% cleavage of whole-length transcripts. Two transcript editing sites are 81% and 61% edited in rf3rf3 lines, while these sites are 41% and 10% edited in the remaining whole-length transcripts in an Rf3Rf3 line. RNA editing and processing efficiency in F1 progeny were similar to the Rf3Rf3 parent, and analyses of backcross progeny indicated that all rf3rf3 lines were characterized by high editing efficiency. We postulate that highly edited transcripts within the population are quickly processed in lines carrying Rf3, generating a residual population of poorly edited transcripts. Thus, action of Rf3 may have no direct affect on RNA editing, and may be dependent on a substrate of highly edited transcripts. These data indicate a potentially novel role of RNA editing in gene expression through an influence on the efficiency of transcript processing.</abstract><cop>United States</cop><pmid>9644206</pmid><doi>10.1007/s002940050356</doi><tpages>8</tpages></addata></record> |
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| ispartof | Current genetics, 1998-06, Vol.33 (6), p.429-436 |
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| subjects | Amino Acid Sequence Base Sequence Binding Sites - genetics c-to-u rna editing Cell Nucleus - chemistry Cell Nucleus - genetics complementary DNA cytoplasmic male sterility DNA, Mitochondrial - genetics Edible Grain - chemistry Edible Grain - genetics Edible Grain - physiology Fertility - genetics Fertility - physiology Genes, Plant - genetics genomics Heterozygote line differences male fertility messenger RNA Mitochondria - chemistry Mitochondria - genetics mitochondrial DNA molecular conformation Molecular Sequence Data Molecular Structure Nuclear Proteins - genetics Nuclear Proteins - physiology nucleases Nucleic Acid Conformation Open Reading Frames - genetics Plant Proteins - genetics Plant Proteins - physiology protein secondary structure restorer genes rf3 gene RNA - genetics RNA - metabolism RNA editing RNA Editing - genetics RNA Editing - physiology RNA, Mitochondrial Sorghum bicolor Transcription, Genetic - genetics |
| title | Interaction of mitochondrial RNA editing and nucleolytic processing in the restoration of male fertility in sorghum |
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